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Air quality is used worldwide to confirm the current status of air pollution level and associated health risks to the public. Several air pollutants reach very high concentrations in many regions across India. In this study, air pollutants were measured in an urban city of Coimbatore, Tamil Nadu, Southern India, during 2013 to 2014 based on season and location, and the influence of meteorological factors. Air pollutants (PM10, PM2.5, SO2, NO2, CO, and O3) across eight locations including industrial, residential, traffic, and commercial areas were assessed. The results showed that PM10, PM2.5, and CO were the most serious pollutants and their average concentrations ranged from 65.5 to 98.6 μg/m3, 27.6 to 56.9 μg/m3, and 1.58 to 8.21 mg/m3, respectively, among various locations. Significantly higher concentration of air pollutants was recorded in industrial areas followed by traffic and commercial areas. Comparatively higher mean concentration of O3 (2.22 ± 0.75 μg/m3) and CO (7.73 ± 1.86 mg/m3) was recorded during the summer season, whereas the concentration of PM10 (80.3 ± 24.4 μg/m3), PM2.5 (45.1 ± 17.7 μg/m3), SO2 (7.86 ± 1.55 μg/m3), and NO2 (13 ± 1.81 μg/m3) was higher in southwest monsoon. Ozone (O3) and CO positively correlated with temperature and negatively correlated with relative humidity. The level of PM10, PM2.5, and CO concentrations exceeded the National Ambient Air Quality Standards (NAAQS) guidelines. The present study’s results emphasize the need of effective air pollution control in Coimbatore. Precautionary measures to be taken to avoid exposure of air pollutants to the public and minimize pollutants. This study further suggests an investigation on the adverse impact on human health and environment using appropriate risk analysis techniques.

Fluorides are emitted in both gaseous and particle forms in the industrial sector. However, studies usually only report total fluoride content. Therefore, the study aimed to assess the particulate, gaseous fluoride and correlate it with the respirable dust particles in Single Super Phosphate (SSP), Granular Single Super Phosphate (GSSP), and administration divisions of the industry. Respirable dust particles, particulate fluoride, and hydrogen fluoride in the work environment were collected on a filter cassette containing an MCE filter paper (0.8 micron 37-mm) and Na2CO3 impregnated backup pad, respectively, using a personal sampler. The fluoride samples were analyzed using Ion Selective Electrode (ISE) and expressed as milligrams per meter cube (mg.m-3). The respirable dust, particulate, and gaseous fluoride content were found to have statistically significant differences (p<0.001) between the divisions (SSP, GSSP, and administration) in the static monitoring, whereas, in the case of personal monitoring, no significant differences were observed. Average airborne respirable, particulate, and gaseous fluoride levels in static monitoring were 1.37, 1.03, 0.20 mg.m-3, 0.018, 0.008, 0.001 mg.m-3, and 0.808, 0.403, 0.026 ppm in SSP, GSSP and administration respectively, whereas in personal monitoring the average respirable, particulate and gaseous fluoride concentrations were 1.18, 0.85, 0.30 mg.m-3, 0.0013, 0.007, 0.002 mg.m-3 and 0.356, 0.258, 0.011 ppm in SSP, GSSP and administration respectively. The present study observed that the levels of fluoride decreased with an increase in distance from SSP, followed by GSSP and administration. It indicates that the fluoride exposure was inversely proportional to the distance of the source. This study outcome will help to design a policy and intervention to mitigate fluoride exposure among workers.

Assassin bugs are terrestrial predators belonging to the family Reduviidae. Among the 24 subfamilies, harparctorinae is predominant and extensively investigated for biocontrol applications compared to the others subfamilies in Reduviidae. Being natural enemies of phytophagous insects, understanding the phylogeny of these predatory bugs can precise the selection of candidates to employ in insect pest management. In addition to morphological systematics, complete mitochondrial genome sequences provide great insights into the phylogeny for resolving evolutionary complexity. Complete mitochondrial genomes of four potential predatory harpactorinae and one outgroup triatominae were retrieved from NCBI GenBank database. Comparative analysis of the five mitogenomes and the nucleotide sequence between nad1 and nad2 genes were selected as the best option to distinguish. The nucleotide sequence between nad1- nad2 are found to be biased towards A and T similar to their respective complete mitogenomes. Tajima’s test of neutrality suggest that the evolutionary selection at nad1-nad2 was parallel to the complete mitogenome and showed positive and significant (p > 0.1) with high nucleotide diversity. Unequal evolutionary rate at nad1-nad2 between lineages observed in Tajima’s relative rate test and proved the nucleotide sequences of nad1-nad2 between species are highly variable. Comparing the phylogenetic trees generated using the complete mitogenomes and nad1-nad2 genes uncovered the correlation between the trees and having identical branches with varying bootstrap values. Conventionally the highly conserved protein-coding cox1 gene is used for molecular taxonomy whereas this study provides an additional and/or a possible alternative molecular marker for genetic comparative test (the nucleotide sequence between nad1-nad2) to understand the systematics and phylogeny of Reduviidae. The significant nucleotide diversity, high genetic distance and less genetic similarity of the sequence between nad1-nad2 genes among the species studied, Agriosphodrus dohrni, Rhynocoris fuscipes, Scipinia horrida, and Velinus nodipes undoubtedly propose the possible utilization of nad1-nad2 region as distinguishable molecular marker.

Entomopathogenic fungi (EPF) are the microorganisms thatspecifically infect and often kill insects and other arthropods. EPF is themost effective biocontrol agent against insects in the natural ecosystem whichcould be an effective alternative to chemical insecticides in bio-intensivepest management. Beauveria bassiana, one of the most prevalentsoil-borne entomopathogens, has virulence on insect pests. The present study isaimed to evaluate the pathogenicity of a native isolate of the entomopathogenicfungus B. bassiana isolated from the soil samples of a cotton field(Kuthukkal) in the Tirunelveli district of Tamil Nadu against Dysdercuscingulatus. Bio-efficacy trials were carried out with six differentconcentrations viz., 4.6 × 103,1.5× 104, 5.0 × 105, 2.7 × 106, 3.2 × 107,and 2.8 x 108 (spores/mL) in all the five nymphal instars and theadults of D. cingulatus. A 100% mortality was observed in higherconcentrations 2.8 x 108 (spores/mL) at 120hrs after treatment. The resultsof the present study show that the isolate seems to be highly promising in thepest management of D. cingulatus.

Extend the longevity of diesel in the world there is a need for identify ing alternatives sources. Biodiesel is the one of the best alternatives to overcome the fuel crises, day to day fuel price arise and environment emission concerns. In this study, the performance of the engine and emission has been studied. The raw non-edible waste cooking oil and Jatropha oils are equally blended 1:1 ratio is used to convert the biodiesel with methanol and alkaline catalyst KOH (Potassium Hydroxide) by transesterification process. Evaluated the physical properties of WJB value as per ASTM Standards. Nano additives magnesium oxide and graphene oxide were added 2:1 ratio mixed with biodiesel and diesel blend. The 4-stroke diesel engine used to find emission and performance of the bio-diesel blend WJB10, WJB20, WJB30,WJB40 and compared with mean diesel. The performance test ,combustion test and emission result were found lesser fuel consumption with brake thermal efficiency and reduced HC,CO,CO2 and NOx in WJB30 fuel blend compared to diesel.

To describe exposure level of organochlorine pesticides (OCP) among workers occupationally engaged in agriculture and sheep wool associated jobs, the present study was carried out in rural neighborhood of Bangalore city, India. Thirty participants were interviewed and obtained informed consent before blood sample collection. The maximum concentrations of OCP were detected in blood samples of agriculture workers than sheep wool workers. Among the metabolites of HCH and DDT, lindane (γ-HCH) and p,p’ -DDE were the most contributed to the total OCP. There were no differences in pesticide residues found between sex and work groups. It was observed that about 30% of samples exceeded the tolerance limits of 10 μg/L prescribed for HCH under the prevention of food adulteration act. Therefore, the present study recommends continuous monitoring with larger sample size.

Background: Cholinesterase determination indicates whether the person has been under pesticide exposure is not. It is recommended that the worker′s cholinesterase level should be assessed for workers at a pesticide applied region. Hence, cholinesterase activities in blood samples of agricultural workers exposed to vegetables and grape cultivation with age matched, unexposed workers, who never had any exposure to pesticides, were estimated. Methods: The detailed occupational history and lifestyle characters were obtained by questionnaire. Cholinesterase activity was determined by the method of Ellman as modified by Chambers and Chambers. Results: AChE was ranging from 1.65 to 3.54μmoles/min/ml in exposed subjects where as it was ranged from 2.22 to 3.51μmoles/min/ml in control subjects. BChE activity was ranging from 0.16 to 5.2μmoles/min/ml among exposed subjects, where as it was ranged from 2.19 to 5.06μmoles/min/ml in control subjects. The results showed statistically significant reduction in enzyme activities (AChE 14%; BChE 56%) among exposed subjects. Conclusion: It was concluded that the reduction in cholinesterase activity may lead to varieties of effects. Hence it is compulsory to use protective gadgets during pesticide spray. Further a continuous biomonitoring study is recommended to assess pesticide exposure.

IntroductionMost of the burden estimates on needle stick injury (NSI) from India were either record based or through passive surveillance studies. Thus, an active surveillance study is set-up to estimate incidence of NSI across primary, secondary and tertiary healthcare facilities.Material and MethodsThis prospective study is being carried out in primary, secondary and tertiary health facilities in Karnataka, India, where healthcare workers (HCWs) are recruited and followed-up once in a month telephonically, for a period of 1 year, to estimate the number of NSI occurring at workplace. At baseline, information on hospital safety climate and self-reported compliance with universal precautions are being collected using pre-tested questionnaire. Blood samples are collected at recruitment and planned to be collected at six-months following the NSI, to assess for seroconversion to HIV, HBV and HCV following NSI. Costs towards laboratory testing for blood borne infections and post exposure prophylaxis are estimated. A sample size of 400 HCWs is estimated for the study.ResultsBetween August and November 2022, 168 HCWs have been recruited from 3 Primary Health Centres and 4 Government General Hospitals in Karnataka, and are under monthly follow-up. Recruitment of remaining 232 HCWs from 1 Government Hospital and 2 Medical College hospitals is expected to complete by January 2023. Expected outcomes of this study include – incidence of NSI across HCWs and level of health facility, proportion of HCWs seroconverted to HBV or HCV or HIV following NSI and median cost of managing NSI in healthcare provider and HCW perspective. Interim analysis will be performed by February 2023 for aforementioned outcomes.ConclusionResults of this study is expected to strengthen policy on safe handling of needles/sharps for HCWs at respective hospitals and also at state level.

Since 2011 under Indian law broadcasts and films that show tobacco use must include health messages as flashing subtitles for the duration of the scene, with announcements before, during, and after. But, asks Balaji Ravichandran, does this deter would-be smokers?