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The Scotian Slope in the North Atlantic Ocean extends for ∼ 500 km along the coast of Nova Scotia, Canada. Its surface sediments host microbial communities, which respond to complex geochemical drivers that not only include communication with the overlying water column, but also potential advection from deeper basinal fluids. Archaea are fundamental components of these communities, and their lipids act as important historical indicators of environmental geochemical change and microbial interactions within marine sediments. This study evaluates the spatial abundance and diversity of archaeal lipids preserved in shallow Scotian Slope sediments to better understand processes. Seventy-four sediment samples from 32 gravity and piston cores, reaching a maximum of 9 m below seafloor (m b.s.f.) were collected during three survey cruises. In total, 14 archaeal lipid classes comprising 42 unique compounds were detected. The lipid distributions reflect a high contribution of anaerobic methanotrophic (ANME) archaeal groups, such as ANME-1 to -3. Hierarchical cluster analysis and principal components analysis were used to show varying contributions of four lipid classes that included distinct assemblages of intact polar lipids (IPLs), core lipids (CLs), and their degradation products (CL-DPs). IPL to CL and CL to CL-DP turnover rates were estimated for the various lipid classes. Four stratigraphically distinct archaeal lipidomes were observed. The first, reflects a unique community influenced by a nearby cold seep. Three additional ambient sediment lipidomes were detected with overlapping depth intervals. These lipidomes contained varying abundances of IPL, CL, and CL-DPs, which likely mark geochemically controlled, microbial community variations that are further accompanied by a systematic increase to the stockpile of diagenetically altered lipids. The ambient sediment lipidomes appear to be highly spatially conserved across the latitudinal extent of the study area marking a resolvable shallow sediment lipostratigraphy that occupies a sediment stratigraphy that spans ∼ 27 000±4000 years of basin evolution for the Scotian Slope.