Explore the vast range of titles available.

Tissue injury to skin diminishes miR-200b in dermal fibroblasts. Fibroblasts are widely reported to directly reprogram into endothelial-like cells and we hypothesized that miR-200b inhibition may cause such changes. We transfected human dermal fibroblasts with anti-miR-200b oligonucleotide, then using single cell RNA sequencing, identified emergence of a vasculogenic subset with a distinct fibroblast transcriptome and demonstrated blood vessel forming function in vivo. Anti-miR-200b delivery to murine injury sites likewise enhanced tissue perfusion, wound closure, and vasculogenic fibroblast contribution to perfused vessels in a FLI1 dependent manner. Vasculogenic fibroblast subset emergence was blunted in delayed healing wounds of diabetic animals but, topical tissue nanotransfection of a single anti-miR-200b oligonucleotide was sufficient to restore FLI1 expression, vasculogenic fibroblast emergence, tissue perfusion, and wound healing. Augmenting a physiologic tissue injury adaptive response mechanism that produces a vasculogenic fibroblast state change opens new avenues for therapeutic tissue vascularization of ischemic wounds. Here, the authors report on the discovery of physiological vasculogenic fibroblasts capable of forming functional blood vessels. In vivo tissue reprogramming triggered by topical tissue nanotransfection (TNT) of a single anti-miR-200b oligonucleotide achieved therapeutic tissue vascularization.

Understanding the factors that promote civic engagement among emerging adult college students is crucial, especially considering its association with positive youth development. The current study examined ethnic-racial identity (ERI) exploration, resolution, and affirmation as mediators of the relation between cultural socialization and civic engagement. Additionally, the extent to which students were raised in predominantly minoritized neighborhoods (i.e., predominantly minoritized neighborhood racial composition; PMNRC) was included as a moderator of the associations between cultural socialization and ERI components. Last, we tested whether findings varied based on students’ ethnic-racial backgrounds (i.e., differences in the model for Asian, African American, Latinx, and Multiracial students of color; N = 1036). Results indicated that there was a significant mediation path, such that cultural socialization predicted greater ERI exploration and, in turn, greater civic engagement. Cultural socialization was also positively associated with greater ERI resolution and affirmation. The racial composition of the neighborhoods that individuals were raised in was not significantly associated with any ERI component; however, PMNRC moderated the relation between cultural socialization and ERI affirmation. Specifically, cultural socialization predicted more ERI affirmation at higher levels of PMNRC, but this relation was not significant at low levels of PMNRC. There were no significant ethnic-racial differences in relations we tested in the model. These findings highlight the importance of cultural processes in civic engagement among diverse emerging adults.

Red Snapper Lutjanus campechanus are the most economically important reef fish in the Gulf of Mexico and a heavily targeted fishery. When brought to the surface from deep water, this species often suffers pressure-related injuries collectively known as barotrauma. This trauma results in high discard mortality and has affected recovery of the fishery. In laboratory experiments using hyperbaric chambers, we assessed sublethal effects of barotrauma and subsequent survival rates of Red Snapper after capture events from pressures corresponding to 30 and 60 m deep. We then evaluated the use of rapid recompression and venting to increase survival and improve recovery after release in this controlled environment. Vented fish in simulated surface release and rapid-recompression treatments had 100% survival. Fish released at the surface that were not vented had 67% survival after decompression from 30 m but only 17% survival from 60 m, while nonvented rapidly recompressed fish had 100% survival from 30 m and 83% survival from 60 m. Fish that were vented upon release at the surface showed significantly better ability to achieve an upright orientation and evade a simulated predator. Results showed clear benefits of venting or recompression. Our data also show strong depth effects resulting in increased barotrauma injuries, more impaired reflexes, and greater mortality as depth increases. Overall, our data support venting or rapid recompression as effective tools for alleviating barotrauma symptoms, improving predator evasion, and increasing overall survival.

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.

Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.

The Heavy Photon Search experiment (HPS) at the Thomas Jefferson National Accelerator Facility searches for electro-produced dark photons. We report results from the 2016 Engineering Run consisting of 10608/nb of data for both the prompt and displaced vertex searches. A search for a prompt resonance in the \\(e^+e^-\\) invariant mass distribution between 39 and 179 MeV showed no evidence of dark photons above the large QED background, limiting the coupling of ^2 10^-5, in agreement with previous searches. The search for displaced vertices showed no evidence of excess signal over background in the masses between 60 and 150 MeV, but had insufficient luminosity to limit canonical heavy photon production. This is the first displaced vertex search result published by HPS. HPS has taken high-luminosity data runs in 2019 and 2021 that will explore new dark photon phase space.

The Heavy Photon Search (HPS) experiment is designed to search for a new vector boson \\(A^\\) in the mass range of 20 MeV/\\(c^2\\) to 220 MeV/\\(c^2\\) that kinetically mixes with the Standard Model photon with couplings \\(^2 >10^-10\\). In addition to the general importance of exploring light, weakly coupled physics that is difficult to probe with high-energy colliders, a prime motivation for this search is the possibility that sub-GeV thermal relics constitute dark matter, a scenario that requires a new comparably light mediator, where models with a hidden \\(U(1)\\) gauge symmetry, a \"dark\", \"hidden sector\", or \"heavy\" photon, are particularly attractive. HPS searches for visible signatures of these heavy photons, taking advantage of their small coupling to electric charge to produce them via a process analogous to bremsstrahlung in a fixed target and detect their subsequent decay to \\(e^+ e^-\\) pairs in a compact spectrometer. In addition to searching for \\(e^+ e^-\\) resonances atop large QED backgrounds, HPS has the ability to precisely measure decay lengths, resulting in unique sensitivity to dark photons, as well as other long-lived new physics. After completion of the experiment and operation of engineering runs in 2015 and 2016 at the JLab CEBAF, physics runs in 2019 and 2021 have provided datasets that are now being analyzed to search for dark photons and other new phenomena.

We present an overview of a new integral field spectroscopic survey called MaNGA (Mapping Nearby Galaxies at Apache Point Observatory), one of three core programs in the fourth-generation Sloan Digital Sky Survey (SDSS-IV) that began on 2014 July 1. MaNGA will investigate the internal kinematic structure and composition of gas and stars in an unprecedented sample of 10,000 nearby galaxies. We summarize essential characteristics of the instrument and survey design in the context of MaNGA's key science goals and present prototype observations to demonstrate MaNGA's scientific potential. MaNGA employs dithered observations with 17 fiber-bundle integral field units that vary in diameter from 12\" (19 fibers) to 32\" (127 fibers). Two dual-channel spectrographs provide simultaneous wavelength coverage over 3600-10300 A at R~2000. With a typical integration time of 3 hr, MaNGA reaches a target r-band signal-to-noise ratio of 4-8 (per A, per 2\" fiber) at 23 AB mag per sq. arcsec, which is typical for the outskirts of MaNGA galaxies. Targets are selected with stellar mass greater than 1e9 Msun using SDSS-I redshifts and i-band luminosity to achieve uniform radial coverage in terms of the effective radius, an approximately flat distribution in stellar mass, and a sample spanning a wide range of environments. Analysis of our prototype observations demonstrates MaNGA's ability to probe gas ionization, shed light on recent star formation and quenching, enable dynamical modeling, decompose constituent components, and map the composition of stellar populations. MaNGA's spatially resolved spectra will enable an unprecedented study of the astrophysics of nearby galaxies in the coming 6 yr.

For a variety of reasons, little research has been conducted among adolescents with substance abuse problems. We operate as though data obtained from studies in adults applies to adolescents, but we know that there are critical differences. Thus assumptions about adolescents must be empirically tested. The following are a series of questions compiled by the Commission on Abuse of Alcohol and Other Drugs that we believe should be given high priority.

The effective prevention and treatment of adolescent substance abuse, like that for any public health problem, require a clear understanding of causes and the context in which these causes operate. Research on the origins of adolescent substance abuse is asking many important questions: How do different drugs affect the brains of different adolescents at different stages of maturation? How do environmental conditions increase or decrease the probability of substance abuse? Are there individual sensitivities to drugs of abuse that increase the risk of a substance use disorder? How do social factors produce contagion of drug abuse among adolescents? Are there specific treatments or prevention interventions for specific types of adolescent substance abuse? How do biological, psychological, and social factors account for failed prevention efforts or drug treatment relapse, and how common is it? This promising body of research carries with it the potential to create more effective approaches to the prevention of adolescent substance abuse as well as guiding treatment efforts.