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In 2004, first absolute gravity (AG) measurements were performed on the top of Mt. Zugspitze (2 sites) and at the foot (1 site) and top (1 site) of Mt. Wank. Mt. Wank (summit height 1780 m) and Mt. Zugspitze (2960 m) are about 15 km apart from each other and belong geologically to different parts of the Northern Limestone Alps. Bridging a time span of 15 years, the deduced gravity variations for Zugspitze are in the order of −0.30 μm/s2 with a standard uncertainty of 0.04 μm/s2. The Wank stations (foot and top) show no significant gravity variation. The vertical stability of Wank summit is also confirmed by results of continuous GNSS recordings. Because an Alpine mountain uplift of 1 or 2 mm/yr cannot explain the obtained gravity decline at Zugspitze, the dominating geophysical contributions are assumed to be due to the diminishing glaciers in the vicinity. The modelled gravity trend caused by glacier retreat between epochs 1999 and 2018 amounts to −0.012 μm/s2/yr at both Zugspitze AG sites. This explains more than half of the observed gravity decrease. Long-term variations on inter-annual and climate-relevant decadal scale will be investigated in the future using as supplement superconducting gravimetry (installed in 2019) and GNSS equipment (since 2018).

Atmospheric aerosol particles like mineral dust, volcanic ash and combustion particles can reduce Earth's snow and ice albedo considerably even by very small amounts of deposited particle mass. In this study, a new laboratory method is applied to measure the spectral light absorption coefficient of airborne particles that are released from fresh snow samples by an efficient nebulizing system. Three-wavelength photoacoustic absorption spectroscopy is combined with refractory black carbon (BC) mass analysis to determine the snow mass-specific and BC mass-specific absorption cross sections. Fullerene soot in water suspensions are used for the characterization of the method and for the determination of the mass-specific absorption cross section of this BC reference material. The analysis of 31 snow samples collected after fresh snowfall events at a high-altitude Alpine research station reveals a significant discrepancy between the measured snow mass-specific absorption cross section and the cross section that is expected from the BC mass data, indicating that non-BC light-absorbing particles are present in the snow. Mineral dust and brown carbon (BrC) are identified as possible candidates for the non-BC particle mass based on the wavelength dependence of the measured absorption. For one sample this result is confirmed by environmental scanning electron microscopy and by single-particle fluorescence measurements, which both indicate a high fraction of biogenic and organic particle mass in the sample.

In peri- and paraglacial regions, water plays a critical role in the hydrological cycle and slope stability. However, hydrological models often overlook water infiltration into bedrock due to limited knowledge of groundwater dynamics at high elevations. Although the link between water presence and rock slope failures is evident in many cases, proof of hydrostatic pressure buildup at depth is scarce, highlighting another significant research gap. This study aims to decipher the hydrological dynamics and empirically derive hydrostatic pressures in deep bedrock. We present unique decennial meteorological data, snowmelt modeling, and discharge measurements from two rock fractures in a tunnel located at ≈ 55 m depth under the permafrost-affected Zugspitze ridge (2815–2962 m a.s.l.). We developed an empirical hydraulic model and detected flow anomalies by comparing inputs (i.e., snowmelt and rainfall) and outputs (i.e., discharge from fractures, baseflow, and no-flow events). Results show continuous flow during snowmelt and discontinuous events during summer months. Hydraulic conductivities are in the order of 10−4 m s−1, with variations according to the saturation. Extreme events are likely to fully saturate the fractures and increase their interconnectivity, producing discharges up to 800 L d−1 and 58 L h−1 from one single fracture. Hydrostatic pressures calculated implementing Darcy's falling-head law are 27 ± 6 m during average snowmelt and 40 ± 10 m for extreme events. These pressure levels can weaken or even destabilize rock slopes in rapidly warming alpine environments. With ongoing climate changes, water relevance is expected to increase, with impacts that have yet to be fully assessed. This study advances the understanding of alpine hydrology and geomorphology by providing new insights into deep groundwater processes and their implications for slope stability.

GFZ (German Research Centre for Geosciences) set up the Zugspitze Geodynamic Observatory Germany with a worldwide unique installation of a superconducting gravimeter at the summit of Mount Zugspitze on top of the Partnach spring catchment. This high alpine catchment is well instrumented, acts as natural lysimeter and has significant importance for water supply to its forelands, with a large mean annual precipitation of 2080 mm and a long seasonal snow cover period of 9 months, while showing a high sensitivity to climate change. However, regarding the majority of alpine regions worldwide, there is only limited knowledge on temporal water storage variations due to sparsely distributed hydrological and meteorological sensors and the large variability and complexity of signals in alpine terrain. This underlines the importance of well-equipped areas such as Mount Zugspitze serving as natural test laboratories for improved monitoring, understanding and prediction of alpine hydrological processes. The observatory superconducting gravimeter, OSG 052, supplements the existing sensor network as a novel hydrological sensor system for the direct observation of the integral gravity effect of total water storage variations in the alpine research catchment at Zugspitze. Besides the experimental set-up and the available data sets, the gravimetric methods and gravity residuals are presented based on the first 27 months of observations from 29 December 2018 to 31 March 2021. The snowpack is identified as being a primary contributor to seasonal water storage variations and, thus, to the gravity residuals with a signal range of up to 750 nm s−2 corresponding to 1957 mm snow water equivalent measured with a snow scale at an altitude of 2420 m at the end of May 2019. Hydro-gravimetric sensitivity analysis reveal a snow–gravimetric footprint of up to 4 km distance around the gravimeter, with a dominant gravity contribution from the snowpack in the Partnach spring catchment. This shows that the hydro-gravimetric approach delivers representative integral insights into the water balance of this high alpine site.

To assist atmospheric monitoring at high-alpine sites, a statistical approach for distinguishing between the dominant air masses was developed. This approach was based on a principal component analysis using five gas-phase and two meteorological variables. The analysis focused on the Schneefernerhaus site at Zugspitze Mountain, Germany. The investigated year was divided into 2-month periods, for which the analysis was repeated. Using the 33.3 % and 66.6 % percentiles of the first two principal components, nine air mass regimes were defined. These regimes were interpreted with respect to vertical transport and assigned to the BL (recent contact with the boundary layer), UFT/SIN (undisturbed free troposphere or stratospheric intrusion), and HYBRID (influences of both the boundary layer and the free troposphere or ambiguous) air mass classes. The input data were available for 78 % of the investigated year. BL accounted for 31 % of the cases with similar frequencies in all seasons. UFT/SIN comprised 14 % of the cases but was not found from April to July. HYBRID (55 %) mostly exhibited intermediate characteristics, whereby 17 % of the HYBRID class suggested an influence from the marine boundary layer or the lower free troposphere. The statistical approach was compared to a mechanistic approach using the ceilometer-based mixing layer height from a nearby valley site and a detection scheme for thermally induced mountain winds. Due to data gaps, only 25 % of the cases could be classified using the mechanistic approach. Both approaches agreed well, except in the rare cases of thermally induced uplift. The statistical approach is a promising step towards a real-time classification of air masses. Future work is necessary to assess the uncertainty arising from the standardization of real-time data.

Whereas bacterial expression systems are widely used for production of uniformly or selectively (15)N-labeled proteins the usage of the baculovirus expression system for labeling is limited to very few examples in the literature. Here we present the complete formulations of the two insect media, IML406 and 455, for the high-yield production of selectively (15)N-labeled proteins in insect cells. The quantities of (15)N-amino acids utilized in the production of labeled GST were similar in the case of bacterial and viral expression. For the most studied amino acids essential for insect cells the (15)N-HSQC spectra, recorded with GST labeled in insect cells, showed no cross labeling and provided therefore spectra of better quality compared to NMR spectra of GST expressed in E. coli. Also in the case of amino acids not essential for Sf9 cells we were able to label a defined number of amino acid species. Therefore the selective labeling using the baculovirus expression vector system represents a complement or even an alternative to the bacterial expression system. Based on these findings we can provide a first simple overview of the network of the amino acid metabolism in E. coli and insect cells focused on nitrogen. For some amino acids the expression of labeled proteins in insect cells can replace the cell-free protein expression.

Cyclase-associated proteins (CAPs) are highly conserved, ubiquitous actin binding proteins that are involved in microfilament reorganization. The N-termini of CAPs play a role in Ras signaling and bind adenylyl cyclase; the C-termini bind to G-actin. We report here the NMR characterization of the amino-terminal domain of CAP from Dictyostelium discoideum (CAP(1-226)). NMR data, including the steady state (1)H-(15)N heteronuclear NOE experiments, indicate that the first 50 N-terminal residues are unstructured and that this highly flexible serine-rich fragment is followed by a stable, folded core starting at Ser 51. The NMR structure of the folded core is an alpha-helix bundle composed of six antiparallel helices, in a stark contrast to the recently determined CAP C-terminal domain structure, which is solely built by beta-strands.