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Background While cervical cancer (CC) is increasingly detected at earlier stages due to improved screening, clinical dilemmas persist in stratifying precancerous lesions and managing advanced disease. Major histocompatibility complex-related genes (MHCRGs), which regulate antigen presentation and immune surveillance, may serve as promising biomarkers for prognosis and therapeutic guidance in CC. Methods We identified MHCRGs via literature review and constructed a prognostic signature using LASSO and multivariate Cox regression based on TCGA-CESC data. Bioinformatic analyses assessed associations between the MHCRG signature, immune cell infiltration, immunotherapy response, and drug sensitivity. Validation was conducted using external GEO datasets (GSE29570, GSE63514), and further confirmed through qRT-PCR and Western blot in clinical CC samples. Functional assays, including gene knockdown in CC cell lines, were performed to explore the biological roles of key MHCRGs in tumor progression. Results A five-gene signature (CANX, HLA-DMB, HLA-DPB1, PSMB6, PSMB7) was identified as predictive of prognosis, immune infiltration, and therapeutic responsiveness. CANX was significantly upregulated, while HLA-DMB and HLA-DPB1 were downregulated in high-risk groups across all datasets and clinical tissues. qRT-PCR and WB results aligned with bioinformatic predictions. Functional experiments demonstrated that silencing CANX suppressed CC cell proliferation and invasion. These findings support the oncogenic role of CANX and the immune-regulatory potential of the other MHCRGs. The MHCscore remained an independent prognostic factor in multivariate analysis. Conclusions We propose a robust MHCRG-based gene signature, validated through both bioinformatic analyses and in vitro functional experiments. These findings offer translational potential for improving risk stratification and guiding immunotherapy in CC patients.

Intravenous leiomyomatosis (IVL) is a histologically well differentiated smooth muscle tumor with aggressive behavior, capable of extending throughout the venous system. Understanding how IVL occurs and develops is really important for diagnosing and treating it. Unfortunately, because IVL is quite rare, there aren’t many comprehensive studies available. In our research, we carried out an extensive multi-omics study, gathering tissue samples from IVL cases, uterine fibroid, and normal myometrium. The single-cell RNA sequencing analysis revealed a notable difference in cell composition between IVL and uterine fibroid. Additionally, H&E staining demonstrated more frequent hydropic changes and hyalinization in IVL tissues, along with a reduced vascular density compared to both normal myometrium and uterine fibroid. In our proteomics analysis of eight paired samples of IVL and normal myometrium fresh frozen tissue, we identified proteins that were differentially expressed, mainly related to focal adhesions and regulation of the actin cytoskeleton. The most frequently involved chromosomes included deletions in 10q22.2, 10q24.32, 13q14, and 13q21-31. Correlation analyses highlighted chromosome 10q as the most frequent cytoband, with corresponding proteins involved in regulating focal adhesions and the cytoskeleton. Integrated analysis between pathological and clinical characteristics indicated that chromosome 10q deletion and vascular morphology in IVL could serve as important markers predicting aggressive behavior. Our study sheds light on the pathological and molecular changes linked to IVL, which could pave the way for new treatment approaches.

Due to the excellent characteristics of fluorescence-based imaging, such as non-invasive detection of biomarkers in vitro and in vivo with high sensitivity, good spatio-temporal resolution and fast response times, it has shown significant prospects in various applications. Compounds with both biological activities and fluorescent properties have the potential for integrated diagnosis and treatment application. Alectinib and Rilpivirine are two excellent drugs on sale that represent a clinically approved targeted therapy for ALK-rearranged NSCLC and have exhibited more favorable safety and tolerance profiles in Phase III clinical trials, ECHO and THRIVE, respectively. The optical properties of these two drugs, Alectinib and Rilpivirine, were deeply explored, firstly through the simulation of molecular structures, electrostatic potential, OPA/TPA and emission spectral properties and experiments on UV-vis spectra, fluorescence and cell imaging. It was found that Alectinib exhibited 7.8% of fluorescence quantum yield at the 450 nm excited wavelength, due to a larger electronic transition dipole moment (8.41 Debye), bigger charge transition quantity (0.682 e) and smaller reorganization energy (2821.6 cm−1). The stronger UV-vis spectra of Rilpivirine were due to a larger electron–hole overlap index (Sr: 0.733) and were also seen in CDD plots. Furthermore, Alectinib possessed obvious active two-photon absorption properties (δmaxTPA* ϕ = 201.75 GM), which have potential TPA imaging applications in bio-systems. Lastly, Alectinib and Rilpivirine displayed green fluorescence in HeLa cells, suggesting the potential ability for biological imaging. Investigation using theoretical and experimental methods is certainly encouraged, given the particular significance of developing integrated diagnosis and treatment.

Large Li2O2 aggregations can produce high‐capacity of lithium oxygen (Li‐O2) batteries, but the larger ones usually lead to less‐efficient contact between Li2O2 and electrode materials. Herein, a hierarchical cathode architecture based on different discharge characteristics of α‐MnO2 and Co3O4 is constructed, which can enable the embedded growth of large Li2O2 aggregations to solve this problem. Through experimental observations and first‐principle calculations, it is found that α‐MnO2 nanorod tends to form uniform Li2O2 particles due to its preferential Li+ adsorption and similar LiO2 adsorption energies of different crystal faces, whereas Co3O4 nanosheet tends to simultaneously generate Li2O2 film and Li2O2 nanosheets due to its preferential O2 adsorption and different LiO2 adsorption energies of varied crystal faces. Thus, the composite cathode architecture in which Co3O4 nanosheets are grown on α‐MnO2 nanorods can exhibit extraordinary synergetic effects, i.e., α‐MnO2 nanorods provide the initial nucleation sites for Li2O2 deposition while Co3O4 nanosheets provide dissolved LiO2 to promote the subsequent growth of Li2O2. Consequently, the composite cathode achieves the embedded growth of large Li2O2 aggregations and thus exhibits significantly improved specific capacity, rate capability, and cyclic stability compared with the single metal oxide electrode. Embedded growth of Li2O2 is realized through a scientific fabrication of α‐MnO2/Co3O4 cathode architecture, of which α‐MnO2 plays the role of initially nucleation sites for Li2O2 while Co3O4 produces plenty of dissolved LiO2, inducing the formation of large Li2O2 aggregations with embedded structure, and thus showing remarkably improved electrochemical performance of Li‐O2 battery.

We have theoretically investigated two series of cyclometalated Pt(II) complexes, a series [Pt (C, N, N) Cl] and b series [Pt (C, N, N pyrazolyl ) Cl]. The geometrical and electronic structures are calculated at the ECP60MWB//6-31G*(H, C, Cl, N, S) basis set level using DFT method; one-photon absorption (OPA) properties are calculated by using both TDDFT and ZINDO methods and two-photon absorption (TPA) properties are obtained with the ZINDO/SOS method. The resonance integrals parameters ( β sp and β d ) for Pt are adjusted to −1 and −28.5 eV, respectively, to make max OPA wavelength calculated by ZINDO closest to the experimental data and TDDFT results. The calculated results indicate the molecule 2b ([Pt (C naphthyl , N, N pyrazolyl ) Cl]) has the biggest potential as outstanding TPA materials because (i) the TPA properties of b series are more outstanding in IR wavelength range, the molecules in b series have good transparencies and possess 1-pyrazolyl-NH that is also available for another metal coordination (e.g., dimerization) and chemical interactions; (ii) when C is C naphthyl in the C, N, N ligand of cyclometalated Pt(II) complexes, the molecules have the best conjugation effect and the best TPA properties.

Large Li 2 O 2 aggregations can produce high‐capacity of lithium oxygen (Li‐O 2 ) batteries, but the larger ones usually lead to less‐efficient contact between Li 2 O 2 and electrode materials. Herein, a hierarchical cathode architecture based on different discharge characteristics of α‐MnO 2 and Co 3 O 4 is constructed, which can enable the embedded growth of large Li 2 O 2 aggregations to solve this problem. Through experimental observations and first‐principle calculations, it is found that α‐MnO 2 nanorod tends to form uniform Li 2 O 2 particles due to its preferential Li + adsorption and similar LiO 2 adsorption energies of different crystal faces, whereas Co 3 O 4 nanosheet tends to simultaneously generate Li 2 O 2 film and Li 2 O 2 nanosheets due to its preferential O 2 adsorption and different LiO 2 adsorption energies of varied crystal faces. Thus, the composite cathode architecture in which Co 3 O 4 nanosheets are grown on α‐MnO 2 nanorods can exhibit extraordinary synergetic effects, i.e., α‐MnO 2 nanorods provide the initial nucleation sites for Li 2 O 2 deposition while Co 3 O 4 nanosheets provide dissolved LiO 2 to promote the subsequent growth of Li 2 O 2 . Consequently, the composite cathode achieves the embedded growth of large Li 2 O 2 aggregations and thus exhibits significantly improved specific capacity, rate capability, and cyclic stability compared with the single metal oxide electrode.

This paper presents a model of RCS characteristic of non-uniform plasma sheltering disk target, by considering the absorption and refraction of electromagnetic wave, and combining graphic electromagnetic and ray tracking methods. The results show that: (1) RCS is reduced more than 10dB at wide-band frequency of electromagnetic wave, when the incident direction of electromagnetic wave is near the vertical surface. (2)For optimization of RCS reduction scheme, it is effective to optimize the relation between the plasma parameters (including electronic density, collision frequency, over-dense target scale) and incident wavelength. When the area of over-dense surface was larger than the target scale, the larger the collision frequency could lead to greater RCS reduction. When the area of over-dense scale was smaller than target, the RCS reduction has to be achieved by increasing electronic density and collision frequency.

We have theoretically investigated two series of cyclometalated Pt（II） complexes, a series [Pt （C, N, N） Cl] and b series [Pt （C, N, Npyrazolyl） Cl]. The geometrical and electronic structures are calculated at the ECP60MWB//6-31G＊（H, C, Cl, N, S） basis set level using DFT method; one-photon absorption （OPA） properties are calculated by using both TDDFT and ZINDO methods and two-photon absorption （TPA） properties are obtained with the ZINDO/SOS method. The resonance integrals parameters （βsp and βd） for Pt are adjusted to -1 and -28.5 eV, respectively, to make max OPA wavelength calculated by ZINDO closest to the experimental data and TDDFT results. The calculated results indicate the molecule 2b （[Pt （Cnaphthyl, N, Npyrazolyl） Cl]） has the biggest potential as outstanding TPA materials because （i） the TPA properties of b series are more outstanding in IR wavelength range, the molecules in b series have good transparencies and possess 1-pyrazolyl-NH that is also available for another metal coordination （e.g., dimerization） and chemical interactions; （ii） when C is CnaphthyI in the C, N, N ligand of cyclometalated Pt（II） complexes, the molecules have the best conjugation effect and the best TPA properties.

Multi-resonance thermally activated delayed fluorescence (MR-TADF) materials have garnered significant research interest owing to their remarkably narrow emission spectra with full width at half maximum (FWHM) below \\(40~\\text{nm}\\), demonstrating substantial advantages over conventional donor-acceptor (D--A) type TADF materials in spectral purity. However, conventional N--B--N resonant framework materials are fundamentally constrained by their intrinsically low reverse intersystem crossing rates (\\(k_{\\text{RISC}} < 10^{3}~\\text{s}^{-1}\\)), presenting a persistent challenge for achieving high-efficiency TADF. This study proposes a triple collaborative design strategy based on CzBN to break through this limitation: (1) Enhance the separation of HOMO and LUMO by \\(\\pi\\)-conjugation expansion and reduce \\(\\Delta E_{\\text{ST}}\\); (2) Introduce O/S heteroatoms to control the excited state charge transfer (CT) characteristics and further reduce \\(\\Delta E_{\\text{ST}}\\); (3) Enhance the spin-orbit coupling (SOC) effect through the synergy of extended \\(\\pi\\)-system and heteroatoms. Based on this, five new MR-TADF molecules were designed and studied. Among them, the \\(k_{\\text{RISC}}\\) of CzBN\\_S reached \\(3.48 \\times 10^{6}~\\text{s}^{-1}\\), two orders of magnitude higher than CzBN, while maintaining \\(\\Delta E_{\\text{ST}} < 0.1~\\text{eV}\\) and FWHM at \\(40~\\text{nm}\\).

The piwi-interacting RNA (piRNA) pathway plays a pivotal role in controlling transposable element (TE) activity, which is crucial for the developmental competence of gametogenesis. Although piRNAs have been studied in golden hamsters and other representative mammals, little is known about the relationship between distinct piRNA populations and their regulatory effects on TEs in human oocytes. In this study, we simultaneously profiled small and long RNA transcriptomes in individual human oocytes across four developmental stages. piRNAs, especially PIWIL3-associated short piRNAs (short-piRNAs), are the predominant small non-coding RNAs during human oogenesis. A marked increase in short-piRNAs after the primordial follicle stage coincided with a global downregulation of TE expression, particularly LINE-1 (L1) and endogenous retroviruses (ERVs). On the other hand, PIWIL1- and PIWIL2-associated long piRNAs (long-piRNAs) were correlated with the silencing of certain specific ERV subfamilies. Genomic-context analyses revealed that highly productive piRNA clusters have evolved asymmetric antisense insertion bias toward L1 and ERVs, contributing to TE families-specific regulation. Our findings highlight the global effect of piRNA-mediated TEs repression, with short-piRNAs acting as the primary and broad-spectrum suppressors, and long-piRNAs providing coordinated ERV-specific silencing. In summary, this study provides a valuable dataset of small and long RNA co-expression landscapes in developing human oocytes and offers insights into the coordinated yet distinct roles of different PIWI/piRNA classes in repressing TEs during human oogenesis.