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Background Triple-negative breast cancer (TNBC) remains clinically challenging due to its aggressiveness, limited targeted therapies, and absence of reliable prognostic biomarkers. Identifying TNBC-specific molecular drivers is critical for improving patient outcomes. Methods We employed an integrated multi-omics approach to analyze tumor and adjacent normal tissues from 4 TNBC and 4 non-TNBC patients via RNA sequencing (RNA-seq) and whole-exome sequencing (WES), complemented by an extensive analysis of 115 TNBC and 976 non-TNBC samples from The Cancer Genome Atlas (TCGA). Prognostic screening was performed to identify potential biomarkers correlated with patient survival. In vitro experimental validation was carried out in MDA-MB-231 TNBC cells, and immunohistochemistry was used to confirm protein expression levels. Results Weighted Gene Co-expression Network Analysis (WGCNA) and comparative WES analysis revealed that TNBC exhibited unique transcriptional modules enriched in DNA replication and heterochromatin organization, distinct somatic mutation landscapes, and alternative splicing patterns. Prognostic screening identified chloride intracellular channel 3 ( CLIC3 ) as the top risk factor for poor survival. In vitro experimental validation showed that CLIC3 knockdown significantly inhibited proliferation (CCK-8, colony formation , invasion and migration in MDA-MB-231 TNBC cells. Immunohistochemistry assay confirmed significantly higher CLIC3 protein expression in TNBC versus non-TNBC tissues. Conclusions CLIC3 is a TNBC-specific prognostic biomarker and therapeutic target. Its inhibition impedes TNBC aggressiveness, providing a foundation for targeted therapy development.

Background Sodium benzoate, a common food preservative, may harm fetuses, which could increase the safety concern for pregnant women, but the knowledge, attitude, and practice (KAP) of food preservatives’ impact on health among pregnant women is limited. This study explored the KAP of pregnant women regarding food preservatives. Methods This cross-sectional study was conducted in Henan Province between March and April 2023 among pregnant women. An investigated-designed questionnaire was administered to collect the demographic characteristics and KAP scores. Scores of < 60%, 60-79%, and ≥  80% were considered poor, moderate, and good, respectively. Results The study collected 515 valid questionnaires [303 (58.83%) < 30-years-old participants] for analysis. The knowledge, attitude, and practice scores were 10.21 ± 2.90 (/17; 60.06%), 29.59 ± 2.73 (/40; 73.98%), and 22.86 ± 3.56 (/30; 76.20%), respectively. The structural equation model (SEM) showed that the knowledge of food preservatives directly affected attitudes (β = 0.168, P  = 0.005) and practice (β = 0.250, P  = 0.021) and indirectly affected practice (β = 0.047, P  = 0.004). The attitudes toward food preservatives directly affected practice (β = 0.280, P  = 0.021). Conclusion Pregnant women in Henan Province have moderate knowledge, attitudes, and practices toward food preservatives. Education interventions are needed to improve the knowledge gaps identified in this study, which could also be encountered in other regions. Trial registration Not applicable.

The main objective of this study was to explore the associations of family relationships and negative life events with depressive symptoms among Chinese adolescents. A cross-sectional study of 3081 middle school students was conducted in Ganzhou City, Jiangxi Province, China. Students were asked to complete questionnaires regarding family relationships, negative life events, and depressive symptoms. A mediation analysis was carried out using a multiple regression analysis and the PROCESS macro method. Of all participants, 19.9% reported experiencing depressive symptoms. The prevalence of depressive symptoms was 13.0% and 29.2% in participants with good and poor parental relationships, and the prevalence of depressive symptoms was 11.4% and 30.9% in participants with closed and alienated parental-child relationships, respectively. Parental relationships, parental-child relationships, and negative life events were positively correlated with depressive symptoms. The effect of parental relationships on depressive symptoms was fully mediated by negative life events (Effect = 0.052, 95% CI = [0.023, 0.082]), while the effect of parent-child relationships on adolescent depressive symptoms was partially mediated by negative life events (Effect = 0.075, 95% CI = [0.048, 0.104]). Our results showed a high prevalence of depressive symptoms in Chinese adolescents. Poor family relationships may have the potential to increase the risk of depressive symptoms, and they could affect depressive symptoms through negative life events.

Cardiac hypertrophy is a common pathological change accompanied by various cardiovascular diseases; however, its underlying mechanisms remain elusive. Mounting evidence indicates that long non-coding RNAs (lncRNAs) are novel transcripts involved in regulating multiple biological processes. However, little is known about their role in regulating cardiac hypertrophy. This study revealed a novel lncRNA4930473A02Rik (abbreviated as lncRNAA02Rik), which showed considerably increased expression in hypertrophic mouse hearts in vivo and angiotensin-II (Ang-II)-induced hypertrophic cardiomyocytes in vitro. Notably, lncRNAA02Rik knockdown partly ameliorated Ang-II induced hypertrophic cardiomyocytes in vitro and hypertrophic mouse heart function in vivo, whereas lncRNAA02Rik overexpression promoted cardiac hypertrophy in vitro. Furthermore, lncRNAA02Rik acted as a competing endogenous RNA by sponging miR-135a, while forced expression of lncRNAA02Rik could repress its activity and expression. Furthermore, forcing miR-135a overexpression exerted a significant protective effect against cardiac hypertrophy by inhibiting the activity of its downstream target TCF7, a critical member of Wnt signaling, and the protective effect could be reversed by AMO-135a. Luciferase assay showed direct interactions among lncRNAA02Rik, miR-135a, and TCF7. Altogether, our study demonstrated that lncRNAA02Rik upregulation could promote cardiac hypertrophy development via modulating miR-135a expression levels and TCF7 activity. Therefore, lncRNAA02Rik inhibition might be considered as a novel potential therapeutic strategy for cardiac hypertrophy.