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"Robinson, Catherine"
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Enteric nervous system modulation of luminal pH modifies the microbial environment to promote intestinal health
The enteric nervous system (ENS) controls many aspects of intestinal homeostasis, including parameters that shape the habitat of microbial residents. Previously we showed that zebrafish lacking an ENS, due to deficiency of the sox10 gene, develop intestinal inflammation and bacterial dysbiosis, with an expansion of proinflammatory Vibrio strains. To understand the primary defects resulting in dysbiosis in sox10 mutants, we investigated how the ENS shapes the intestinal environment in the absence of microbiota and associated inflammatory responses. We found that intestinal transit, intestinal permeability, and luminal pH regulation are all aberrant in sox10 mutants, independent of microbially induced inflammation. Treatment with the proton pump inhibitor, omeprazole, corrected the more acidic luminal pH of sox10 mutants to wild type levels. Omeprazole treatment also prevented overabundance of Vibrio and ameliorated inflammation in sox10 mutant intestines. Treatment with the carbonic anhydrase inhibitor, acetazolamide, caused wild type luminal pH to become more acidic, and increased both Vibrio abundance and intestinal inflammation. We conclude that a primary function of the ENS is to regulate luminal pH, which plays a critical role in shaping the resident microbial community and regulating intestinal inflammation.
Journal Article
Experimental bacterial adaptation to the zebrafish gut reveals a primary role for immigration
All animals live in intimate association with microorganisms that profoundly influence their health and development, yet the traits that allow microorganisms to establish and maintain host associations are not well understood. To date, most investigations aimed at identifying traits required for host association have focused on intrahost niches. Consequently, little is known about the relative contribution of extrahost factors such as environmental growth and survival and immigration into hosts from the external environment, as promoters of host association. To address this, we developed a tractable experimental evolution system that investigates both intra- and extrahost factors contributing to bacterial adaptation to the vertebrate gut. We passaged replicate lines of a zebrafish bacterial isolate, Aeromonas veronii, through populations of germ-free larval zebrafish (Danio rerio), each time using gut-associated Aeromonas populations to inoculate the aquatic environment of the next zebrafish population. We observed rapid increased adaptation to the host in all replicate lines. The initial adaptations present in early-evolved isolates did not increase intrahost fitness but rather enhanced both immigration from the environment and interhost transmission. Only in later-evolved isolates did we find evidence for intrahost-specific adaptations, as demonstrated by comparing their competitive fitness in the host genotype to which they evolved to that in a different genotype. Our results show how selection for bacterial transmission between hosts and their environment can shape bacterial-host association. This work illuminates the nature of selective forces present in host-microbe systems and reveals specific mechanisms of increased host association. Furthermore, our findings demonstrate that the entire host-microbe-environment system must be considered when identifying microbial traits that contribute to host adaptation.
Journal Article
Rembrandt and the inspiration of India
\"Accompanying an exhibition at the J. Paul Getty Museum in Los Angeles, this volume brings together Rembrandt's drawings after Mughal paintings and demonstrates how Rembrandt's contact with Mughal art inspired him to draw in an entirely new, refined style.\"--Provided by publisher.
BOOK
Global Review of Social Indicators used in Protected Area Management Evaluation
Social considerations in conservation are increasingly recognized as important for successful environmental outcomes. However, social measures lack consistency and may underreport key issues. This article analyzes social indicators and well‐being dimensions used in protected area effectiveness tools, with specific attention to local communities and Indigenous peoples’ contexts. Using the Global Protected Area Management Effectiveness database, we reviewed 2,736 indicators from 38 methodologies applied in over 180 countries. We analyzed: (1) representation of human well‐being dimensions, (2) direction of impacts, and (3) level of neutrality in indicators. We found limited diversity and representation of important well‐being dimensions such as health and governance. While impacts on communities and nature are similarly measured, positive wording is used three times more often than negative, which may unintentionally bias evaluations. We recommend using and developing indicators with greater diversity, increased clarity, and reduced bias to enhance management and policy responses for biodiversity and human well‐being.
Journal Article
Coproduction mechanisms to weave Indigenous knowledge, artificial intelligence, and technical data to enable Indigenous-led adaptive decision making: lessons from Australia’s joint managed Kakadu National Park
Most of the planet’s vital ecosystems are managed on lands owned by Indigenous peoples. Indigenous people face many challenges in managing these lands, including rapidly growing threats causing species extinctions and ecosystem losses. In response, many Indigenous groups are looking for ethical ways to use digital technology and data analytical tools to support their existing knowledge practices to solve complex environmental management problems. We draw on an action co-research project to show how a range of knowledge coproduction mechanisms were developed and applied to weave Indigenous knowledge, artificial intelligence (AI), and technical sources to monitor the health of Nardab, a culturally significant and Ramsar-listed wetland in Australia's World Heritage-listed Kakadu National Park. The coproduction mechanisms included: holistic assessments of the health of indicators; a dynamic and creative decision-support tool to adaptively manage a complex system; ongoing monitoring and testing of knowledge used for collaborative action; and Indigenous-led governance of research activities and impact at local and regional scales. It was important for local Bininj traditional owners to determine where and how multiple sources of evidence could or should be used and applied to direct and assess on-the-ground actions as part of this collaborative and cross-cultural knowledge sharing and coproduction process. At Nardab, this required negotiating the evidence from qualitative Indigenous-led assessments of significant sites and quantitative ecological information collected and analyzed from cameras and drone surveys. The coproduction mechanisms developed provided a practical and ethical means of empowering different sources of knowledge for adaptive decision making while respecting and protecting differences in how knowledge is generated, interpreted, and applied.
Journal Article
Mapping Indigenous land management for threatened species conservation: An Australian case-study
Much biodiversity lives on lands to which Indigenous people retain strong legal and management rights. However this is rarely quantified. Here we provide the first quantitative overview of the importance of Indigenous land for a critical and vulnerable part of biodiversity, threatened species, using the continent of Australia as a case study. We find that three quarters of Australia's 272 terrestrial or freshwater vertebrate species listed as threatened under national legislation have projected ranges that overlap Indigenous lands. On average this overlap represents 45% of the range of each threatened species while Indigenous land is 52% of the country. Hotspots where multiple threatened species ranges overlap occur predominantly in coastal Northern Australia. Our analysis quantifies the vast potential of Indigenous land in Australia for contributing to national level conservation goals, and identifies the main land management arrangements available to Indigenous people which may enable them to deliver those goals should they choose to do so.
Journal Article
“Part of a cog, of a system; a system that’s broken”: social workers’ experiences of moral injury in England
Moral injury is the long-term effect of the experience of events that violate deeply held moral beliefs. Social workers in adult care settings are tasked with managing morally complex situations and make decisions that impact the lives of service users. Simultaneously, they adhere to their professional ethical code and organisational standards, potentially giving rise to moral injury. Little is known about these experiences. This qualitative descriptive study aimed to explore social workers' experiences of moral injury.
Remote interviews were conducted with ten social work professionals, in adult services in England. Data were inductively analysed using reflexive thematic analysis.
Three themes were identified: a) System-related impacts; b) Service-related impacts; c) Individual psychological impacts. Social worker moral injury can arise from efforts to uphold professional values whilst navigating limited resources, interprofessional conflicts, and working within a constrained system. Witnessing dehumanising, low-quality, and impersonal care is a major source of moral injury. This is marked by professional disempowerment, sadness, guilt, betrayal, and helplessness.
Recognising these experiences is important. Building psychosocial resilience through formal support is essential for moral repair. Application of the concept for social work, alongside further research, is paramount to adequately support staff.
Journal Article
Cultivation of stable, reproducible microbial communities from different fecal donors using minibioreactor arrays (MBRAs)
Background
Continuous-flow culture models are one tool for studying complex interactions between members of human fecal microbiotas because they allow studies to be completed during an extended period of time under conditions where pH, nutrient availability, and washout of waste products and dead cells can be controlled. Because many of the existing well-validated continuous-flow models are large and complex, we were interested in developing a simpler continuous-flow system that would allow microbial community dynamics to be examined in higher throughput while still maintaining complex microbial communities. To this end, we developed minibioreactor arrays (MBRAs), small volume bioreactors (15 ml) that allow simultaneous cultivation of up to 48 microbial communities in a single anaerobic chamber.
Results
We used MBRA to characterize the microbial community dynamics of replicate reactors inoculated from three different human fecal donors and reactors seeded with feces pooled from these three donors. We found that MBRA could be used to efficiently cultivate complex microbial communities that were a subset of the initial fecal inoculum (15–25 % of fecal OTUs initially observed). After an initial acclimation period of approximately 1 week, communities in each reactor stabilized and exhibited day-to-day variation similar to that observed in stable mouse fecal communities. Replicate reactors were predominately populated by shared core microbial communities; variation between replicate reactors was primarily driven by shifts in abundance of shared operational taxonomic units (OTUs). Consistent with differences between fecal donors, MBRA communities present in reactors seeded with different fecal samples had distinct composition and structure.
Conclusions
From these analyses, we conclude that MBRAs can be used to cultivate communities that recapitulate key features of human fecal communities and are a useful tool to facilitate higher-throughput studies of the dynamics of these communities.
Journal Article