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Despite the widespread use of ecological niche models (ENMs) for predicting the responses of species to climate change, these models do not explicitly incorporate any population‐level mechanism. On the other hand, mechanistic models adding population processes (e.g. biotic interactions, dispersal and adaptive potential to abiotic conditions) are much more complex and difficult to parameterize, especially if the goal is to predict range shifts for many species simultaneously. In particular, the adaptive potential (based on genetic adaptations, phenotypic plasticity and behavioral adjustments for physiological responses) of local populations has been a less studied mechanism affecting species’ responses to climatic change so far. Here, we discuss and apply an alternative macroecological framework to evaluate the potential role of evolutionary rescue under climate change based on ENMs. We begin by reviewing eco‐evolutionary models that evaluate the maximum sustainable evolutionary rate under a scenario of environmental change, showing how they can be used to understand the impact of temperature change on a Neotropical anuran species, the Schneider's toad Rhinella diptycha. Then we show how to evaluate spatial patterns of species’ geographic range shift using such models, by estimating evolutionary rates at the trailing edge of species distribution estimated by ENMs and by recalculating the relative amount of total range loss under climate change. We show how different models can reduce the expected range loss predicted for the studied species by potential ecophysiological adaptations in some regions of the trailing edge predicted by ENMs. For general applications, we believe that parameters for large numbers of species and populations can be obtained from macroecological generalizations (e.g. allometric equations and ecogeographical rules), so our framework coupling ENMs with eco‐evolutionary models can be applied to achieve a more accurate picture of potential impacts from climate change and other threats to biodiversity.

Domestication is an evolutionary process during which we expect populations to progressively adapt to an environment controlled by humans. It is accompanied by genetic and presumably epigenetic changes potentially leading to modifications in the transcriptomic profile in various tissues. Reproduction is a key function often affected by this process in numerous species, regardless of the mechanism. The maternal mRNA in fish eggs is crucial for the proper embryogenesis. Our working hypothesis is that modifications of maternal mRNAs may reflect potential genetic and/or epigenetic modifications occurring during domestication and could have consequences during embryogenesis. Consequently, we investigated the trancriptomic profile of unfertilized eggs from two populations of Eurasian perch. These two populations differed by their domestication histories (F1 vs. F7+–at least seven generations of reproduction in captivity) and were genetically differentiated (FST = 0.1055, p<0.05). A broad follow up of the oogenesis progression failed to show significant differences during oogenesis between populations. However, the F1 population spawned earlier with embryos presenting an overall higher survivorship than those from the F7+ population. The transcriptomic profile of unfertilized eggs showed 358 differentially expressed genes between populations. In conclusion, our data suggests that the domestication process may influence the regulation of the maternal transcripts in fish eggs, which could in turn explain differences of developmental success.

 The leaf of the jaborandi (Pilocarpus microphyllus) is the part of the plant of economic interest, from which the pilocarpine is extracted, therefore, leaf production is the main variable that determines the productivity of this species. Pilocarpus leaf production can be managed during its development phase. Thus, the objective of the present study was to evaluate branch regeneration of jaborandi submitted to two types of cutting/pruning and different cropping systems. Prunings were carried out in jaborandi plants from different accessions from the Janorandi Active Germoplasm Bank (BGA), that were under different cropping systems, full sun and shade. With pruning shears, cuts were made 10 and 20 cm from the branch bifurcations. The results showed that regardless of the distances of the cuts in the jaborandi plants, the prunings benefited new shoots production and the harvest and influence on leaf production could be anticipated without harming the plant’s development. The drastic pruning performed on the species also stimulated efficient regeneration without damaging the plant, resulting in bigger leaf production, regardless of the pruning direction. It was also observed that jaborandi cultivation becomes more productive when it is grown under full sun regardless of the germplasm used, and the leaves can be harvested in all the months of the year.

The rapid urban growth followed by disordered occupation has been generating significant impacts on cities, bringing losses of an economic and social nature that directly interfere with the well-being of the population. In this work, a proposal for local urban infrastructure problems associated with watercourse management is presented, comparing Sustainable Drainage System (SuDS) techniques and Low-Impact Development (LID) concepts with alternative traditional interventions. The study addresses sustainable alternatives to cope with the urbanization of the Cehab’s open channel, which is an important urban watercourse tributary of the Muriaé River, at the municipality of Itaperuna, Rio de Janeiro—Brazil. The multi-criteria decision-making method called Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) was applied here. The results highlighted the better performance of sustainable techniques when compared to the traditional ones, with an overall advantage of the geogrids and geocells for this case study. The obtained TOPSIS coefficients-C for these techniques were higher (0.59488, for Reach 1; and 0.68656, for Reach 2) than those for the others. This research, therefore, presented an important urban watercourse management methodology that can be further applied to guide sustainable investments and help the decision-making associated with the development of territories.

Micropropagation is a plant tissue culture technique widely used for the production of medicinal plants. Lippia dulcis is an aromatic species that has potential therapeutic use. The objective of this study was to evaluate the effects of a natural ventilation system (NVS) on the vegetative growth, anatomical characteristics, production of photosynthetic pigments, and antioxidant defense of Lippia dulcis plantlets grown in vitro. Nodal segments pre-established in vitro were inoculated in flasks with 50 mL of MS culture medium and sealed using lids with one (NVS1), two (NVS2), or four (NVS4) membranes or no membrane (NM). After 45 days, vegetative growth parameters, anatomical characteristics, accumulation of photosynthetic pigments and phenolic compounds, and antioxidant defense responses by nonenzymatic mechanisms were evaluated. The NVS4 group had the highest growth rates, greatest accumulation of photosynthetic pigments, and the most anatomically organized tissues, in addition to the best antioxidant defense response. This natural ventilation system with four membranes offered the best conditions for the growth and development of Lippia dulcis plantlets.Key MessageAlternative membrane system increased dry weight, improve anatomical characteristics, accumulation of photosynthetic pigments and phenolic compounds, and antioxidant defense in micropropagated plantlets.

Dysphania ambrosioides L. is a medicinal plant with anti-helmintic potential. The aim of this study was to evaluate separately the effect of light spectra and elicitors on Dysphania ambrosioides growth and volatile constituents in vitro. Thus, plantlets were first cultured under blue (B), red (R), white, combinations of B:R (1:1, 2:1, 1:2) from LEDs and fluorescent lamps. Secondly, nodal segments were inoculated in the medium supplemented with chitosan (0, 50, 100, 150, and 200 mg L− 1) and salicylic acid (0, 3, 6, 9, and 12 mg L− 1). After 40 days of cultivation, the growth parameters and chemical composition of volatile constituents were evaluated. The light spectra significantly influenced in vitro growth of D. ambrosioides. The best growth occured using white LED or a blue:red combination of 2:1. It was also observed that the blue LEDs inhibited the synthesis of Z-ascaridole, while fluorescent light promoted a greater conversion of α-terpinene into ascaridole. The elicitors, chitosan and salicylic acid had a negative effect on the growth of nodal segments. However, the highest Z-ascaridole content was obtained at 50 to 100 mg L− 1 of chitosan and with 6 to 9 mg L− 1 of salicylic acid. The present study demonstrates that shoots regenerated from nodal segments exposed to different light spectra or on MS medium containing chitosan and salicylic acid can exhibit an altered growth and increased volatile constituents of interest.Key messageElicitors and different wavelengths provided by LEDs and fluorescent lamp were developed for Dysphania ambrosioides, altering growth and increase volatile constituents of interest.

Bagassa guianensis Aubl. is a tree of high timber value (Moraceae family) native to the Amazon. In this study, we characterized the morphoanatomical and biochemical properties of Bagassa guianensis seeds at different levels of fruit control based on biometric, morphoanatomical, color and biochemical analyses. Four stages of seed development have been identified in the fruit of Bagassa guianensis. The seeds presented endosperm, being classified as albuminous. Seeds from fruits with 5 GY 4/4 colors and green pigmentation (stage I) presented fully formed embryos. According to PCA (principal component analysis) data, it was found that all stages of seed dominance had a significant effect and highly correlated with the biochemical properties of the seed. Despite the comparatively low biometric values, high levels of Total Soluble Sugars (3.58%), Total Soluble Proteins (2.12%) and Reducing Sugars (1.43%) were observed in seeds at this stage. On the other hand, the content of amino acids (0.87%) and starch (3.30%) was higher at late maturity. The highest amounts of starch were detected from stage II onwards (31.29 mg/g DM), when the seeds reached physiological maturity. Biochemical and morphological information from seeds are relevant for planning conservation, observation and restoration strategies, and this study on the species B. guianensis is essential.

In conventional in vitro culture, plantlets are kept in closed containers to avoid contamination and drying of the explant. Ventilation inside the containers improves plant growth and affects secondary metabolism, as it modifies the microenvironmental conditions. Therefore, the objective of this study was to evaluate the effect of the use of ventilation systems on the growth characteristics and production of photosynthetic pigments and volatile organic compounds (VOCs) in Aeollanthus suaveolens Mart. ex Spreng. (Lamiaceae) cultured in vitro. Nodal segments containing one pair of leaves were cultured in a conventional system (NMS) and in natural ventilation systems with one (AMS1), two (AMS2) and four (AMS4) porous membranes. At 40 days, the plantlets were evaluated for growth, VOC concentration, and photosynthetic pigment production. The number of porous membranes used in the vial cap affected growth, photosynthetic pigments and VOCs. A higher number of porous membranes (AMS4) led to greater dry weight accumulation, increased production of photosynthetic pigments, and enhanced synthesis of (Z)-β-farnesene. Lower growth and fewer photosynthetic pigments, and increase linalool acetate synthesis were observed in the culture without the use of porous membranes (NMS). The leaf area of plantlets cultivated with the use of four membranes was 3.8 times greater than that of plantlets cultivated without the use of membranes. For the photoautotrophic cultivation of A. suaveolens in vitro, the use of natural ventilation with four membranes is recommended because it promotes better growth, increases the production of photosynthetic pigments and is superior to the conventional sealed system. Key MessageAlternative membrane systems led to differentiation in the contents of the main volatile organic compound and improve anatomical characteristics, accumulation of photosynthetic pigments and dry weight.

We investigated the species richness, endemism, and similarity of the bryoflora (mosses and liverworts) on five Brazilian Amazon mountains (four Tepuis and one rocky outcrop), to determine the floristic relationships between them using presence/absence matrix. Cluster analysis based on the Jaccard index was used to determine similarities between the plant assemblages in five areas. We also performed a principal component analysis to determine which abiotic variables best explained the variations between those mountains. We surveyed the bryophyte species on each of the five mountains to identify the diversity of mosses and liverworts and to answer to the following questions: (1) How many bryophyte species are there in total area (gama diversity) and on each mountain (alpha diversity)? (2) Do mosses and liverworts share patterns of diversity? (3) Can patterns of alpha diversity be used to predict patterns of beta diversity? (4) What are the species distribution patterns? (5) How many endemic species are there in the mountains? and, (6) Is there high similarity among those bryophyte floras? We encountered 425 species, 144 genera, and 51 families of bryophytes, with Lejeuneaceae and Lepidoziaceae being the richest families. A considerable number of floristic novelties were encountered: 18 species new to Brazil; 39 species new to northern Brazil; 21 species new to Amazonas State; and 2 species new to science and only recently described. Over 43% of the bryophytes have a tropical America distribution, approximately 7% are Pantropical, 6.5% are Afro-American, and 3% are endemic to Brazil (6 liverworts and 6 mosses), and 6% are widespread globally. We present a checklist of 425 bryophyte species (144 genera and 51 families) of which 12 are endemic. Patterns of endemism of the bryophyte flora on the Tepuis differ from those exhibited by angiosperms (in the latter three times higher). Thirty-nine of these species were new for the Amazonia domain in Brazil, increasing by 7% the previous total of Amazonian recorded species. The similarity between sites was low, ranging from 3 to 25%. PCA analysis showed temperature variables explaining the greater part of the variance (76.6%) between the mountains. Our results demonstrate that patterns of alpha diversity cannot be used to predict patterns of beta diversity. This paper represents the first evaluation of bryophytes on five Brazilian Amazon mountains, giving insight into their species richness, endemism, and similarity, and providing baseline information for analyzing species’ turnover rates, migration, invasion events, etc. These results also represent an increase in our general knowledge of the Brazilian bryoflora and are relevant to the conservation of bryophyte diversity in the Amazon forest, and represent a contribution toward the targets of the Global Strategy for Plant Conservation.