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The purpose of the present study was to examine the utility of a novel morpheme learning task for indexing typical language abilities in children characterized by diverse language backgrounds. Three groups of 5- to 6-year-old children were tested: monolingual speakers of English, native speakers of Spanish who also spoke English (Spanish-L1 bilinguals), and native speakers of English who also spoke Spanish (English-L1 bilinguals). All children were taught a new derivational morpheme /ku/ marking part-whole distinction in conjunction with English nouns. Retention was measured via a receptive task, and sensitivity and reaction time (RT) data were collected. All three groups of children learned the novel morpheme successfully and were able to generalize its use to untaught nouns. Furthermore, language characteristics (degree of exposure and levels of performance on standardized measures) did not contribute to bilingual children's learning outcomes. Together, the findings indicate that this particular version of the novel morpheme learning task may be resistant to influences associated with language background and suggest potential usefulness of the task to clinical practice.

[Rebecca Cook] has several productions in the works, including a hair and makeup gig for Civic's \"A Child's Christmas in Wales\" and costuming \"Honk!\" for the Spokane Children's Theater. Next spring will be her stage directing debut for the Children's Theater's production of \"Snow White.\" Cook longed to remain in Spokane. Afterward, she wrote a list of priorities. Topping that list was the desire to do theater in Spokane. Photo; Rebecca Cook holds several theater-related jobs, mostly at the Civic Theatre. She teaches various theater classes in town and has a hair and makeup gig for the Civic's \"A Child's Christmas in Wales\" and is doing costuming for \"Honk!\" for the Spokane Children's Theater. Photo by Steve Thompson/The Spokesman-Review

That first venture into sciencefiction painting became [Betsy Mott]'s signature style. She now attends six to eight science fiction conventions and art shows a year as well as renting panel space at about 40 shows in various cities, such as San Jose, Calif., and Atlanta. The quantity of shows pays off for Mott, who earns an average of $200 to $300 per show. The Corner Door isn't the only area where Mott has worked with her family. In 1997, she teamed up with her sister, Bobbie, and her brother, Greg, to publish a historical book on Millwood that featured Mott's sketches of area landmarks, such as the Hutton Settlement. The sketches have gained Mott some local recognition. This year, her drawing of the Van Marter house won a Best of Pencil or Ink Category award at the Spokane County Interstate Fair. \"We have some beautiful landmarks,\" Mott said. \"You want something to remember them by.\"

Currently, [Dan Cummins] performs in nationwide two-man shows with Seattle comedian David Crowe, who has won the Seattle and San Francisco Comedy Competitions. Crowe, who has been a comic for 10 years, saw Cummins perform and decided he had to work with him. \"Dan is the most phenomenal comedic talent to come out of the northwest since the Stone Age,\" Crowe said. \"Anybody can learn the science of comedy, but he has that natural something that you're born with that's so rare. Every now and then you see a performer that you have to see again, and Dan has that.\" 2 color photos; Traveling funny man Millwood comedian take his show on the road 1. (From page V1) Dan Cummins, who grew up in the small town of Riggins, Idaho, and now lives in Millwood, is on his way to the big time. The stand-up comic has performed recently in San Francisco. Photo by Steve Thompson/The Spokesman-Review 2. Dan Cummins, a stand-up comedian, lives upstairs from the Corner Store in Millwood. He's getting ready for the big-time comedy circuit. Photo by Steve Thompson/The Spokesman-Review

While often obvious for macroscopic organisms, determining whether a microbe is dead or alive is fraught with complications. Fields such as microbial ecology, environmental health, and medical microbiology each determine how best to assess which members of the microbial community are alive, according to their respective scientific and/or regulatory needs. Many of these fields have gone from studying communities on a bulk level to the fine-scale resolution of microbial populations within consortia. For example, advances in nucleic acid sequencing technologies and downstream bioinformatic analyses have allowed for high-resolution insight into microbial community composition and metabolic potential, yet we know very little about whether such community DNA sequences represent viable microorganisms. In this review, we describe a number of techniques, from microscopy- to molecular-based, that have been used to test for viability (live/dead determination) and/or activity in various contexts, including newer techniques that are compatible with or complementary to downstream nucleic acid sequencing. We describe the compatibility of these viability assessments with high-throughput quantification techniques, including flow cytometry and quantitative PCR (qPCR). Although bacterial viability-linked community characterizations are now feasible in many environments and thus are the focus of this critical review, further methods development is needed for complex environmental samples and to more fully capture the diversity of microbes (e.g., eukaryotic microbes and viruses) and metabolic states (e.g., spores) of microbes in natural environments.

Accurate detection and genotyping of structural variations (SVs) from short-read data is a long-standing area of development in genomics research and clinical sequencing pipelines. We introduce Paragraph, an accurate genotyper that models SVs using sequence graphs and SV annotations. We demonstrate the accuracy of Paragraph on whole-genome sequence data from three samples using long-read SV calls as the truth set, and then apply Paragraph at scale to a cohort of 100 short-read sequenced samples of diverse ancestry. Our analysis shows that Paragraph has better accuracy than other existing genotypers and can be applied to population-scale studies.

It is widely believed that people living in cities have fewer and poorer daily experiences of nature, and that this contributes to the biodiversity crisis on the basis that if people do not know nature, they will not care for its conservation. In 1978, Pyle coined the term ‘extinction of experience’ to reflect the loss of opportunities to experience nature as urbanization progresses. However, evidence of an emotional disconnect from nature arising from an extinction of experience remains poorly documented. Here we repeat a study conducted in 1996 comprising household surveys and bird surveys in a neighbourhood of Singapore, one of the world's most densely populated cities. We investigate empirically whether experiences of nature, opportunities to experience nature and the emotional connection between people and nature have changed across 22 years. We discover that emotional connection with nature has actually increased among greenspace users and non‐users, while the frequency and duration of greenspace visits remained unchanged. Among greenspace users, the number of different nature elements experienced also remained unchanged and more frequent users demonstrated a stronger emotional connection with nature. We further found an increase in opportunities to experience local nature, as measured by bird species richness and abundance. While we found no evidence for an extinction of experience or an emotional disconnect from nature in this particular setting and timeframe in urban Singapore, similar studies elsewhere are necessary to explore whether there may be geographical, cultural or temporal variations. A free Plain Language Summary can be found within the Supporting Information of this article. A free Plain Language Summary can be found within the Supporting Information of this article.