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One of the most studied aspects of animal communication is the acoustic repertoire difference between populations of the same species. While numerous studies have investigated the variability of bottlenose dolphin whistles between populations, very few studies have focused on the signature whistles alone and the factors underlying differentiation of signature whistles are still poorly understood. Here we describe the signature whistles produced by six distinct geographical units of the common bottlenose dolphin ( Tursiops truncatus ) in the Mediterranean Sea and identify the main determinants of their variability. Particularly, the influence of the region (proxy of genetic distance), the geographic site, and the environmental (sea bottom-related) and demographical (population-related) conditions on the acoustic structure of signature whistles was evaluated. The study provides the first evidence that the genetic structure, which distinguishes the eastern and western Mediterranean bottlenose dolphin populations has no strong influence on the acoustic structure of their signature whistles, and that the geographical isolation between populations only partially affected whistle variability. The environmental conditions of the areas where the whistles developed and the demographic characteristics of the belonging populations strongly influenced signature whistles, in accordance with the “acoustic adaptation hypothesis” and the theory of signature whistle determination mediated by learning.

Growing concerns about the impact of leisure boating on marine ecosystems are particularly relevant for marine mammal species, given their dependence on sound and significant spatial overlap with human activities. Monitoring the effects of leisure boating disturbances on marine ecosystems presents considerable challenges, as it is resource-intensive and may require many years of data collection. However, since species can alter their behavior and daily routines in response to human disturbances, behaviors essential for individual fitness, reproduction, and parental care success—such as acoustic behavior—could serve as bioindicators for assessing the impact of leisure boating. Common bottlenose dolphins ( Tursiops truncatus ) emit acoustic signals, known as signature whistles (SWs), for individual identification, communication, and social bonding. This study aimed to examine how leisure boating influences SW characteristics ( e.g. , fundamental frequencies, frequency modulation, and duration) and whether these effects remain consistent across different social contexts ( e.g. , presence of calves), environmental conditions ( e.g. , seabed habitat type), and behavioral states in a population of bottlenose dolphins inhabiting the northwestern coast of Sardinia (Mediterranean Sea, Italy). The results demonstrate that certain SW characteristics were consistently affected by the presence of boats, regardless of ecological, behavioral, and social differences. These findings suggest that SWs have the potential to serve as reliable bioindicators for boating disturbances. Further research in diverse marine environments and with other dolphin populations is needed to validate and expand upon these findings.

Background Sustainable management requires spatial mapping of both species distribution and human activities to identify potential risk of conflict. The common bottlenose dolphin (Tursiops truncatus) is a priority species of the European Union Habitat Directive, thus, to promote its conservation, the understanding of habitat use and distribution, as well as the identification and spatial trend of the human activities which may directly affect populations traits, is pivotal. Methods A MaxEnt modeling approach was applied to predict the seasonal (from April to September) habitat use of a small population of bottlenose dolphins in the north-western Sardinia (Mediterranean Sea) in relation to environmental variables and the likelihoods of boat and fishing net presence. Then, the overlapping areas between dolphin, fishing net and boat presence were identified to provide insights for the marine spatial management of this area. Results Three of the main factors influencing the seasonal distribution of bottlenose dolphins in the area are directly (boating and fishing) or indirectly (ocean warming) related to human activities. Furthermore, almost half of the most suitable area for dolphins overlapped with areas used by fishing and boating. Finally, relying on fishing distribution models, we also shed light on the potential impact of fishing on the Posidonia oceanica beds, a protected habitat, which received higher fishing efforts than other habitat types. Discussion Modelling the spatial patterns of anthropogenic activities was fundamental to understand the ecological impacts both on cetacean habitat use and protected habitats. A greater research effort is suggested to detect potential changes in dolphin habitat suitability, also in relation to ocean warming, to assess dolphin bycatch and the status of target fish species, and to evaluate sensitive habitats conditions, such as the Posidonia oceanica meadow.

Marine soundscape is the aggregation of sound sources known as geophony, biophony, and anthrophony. The soundscape analysis, in terms of collection and analysis of acoustic signals, has been proposed as a tool to evaluate the specific features of ecological assemblages and to estimate their acoustic variability over space and time. This study aimed to characterise the Capo Caccia-Isola Piana Marine Protected Area (Italy, Western Mediterranean Sea) soundscape over short temporal (few days) and spatial scales (few km) and to quantify the main anthropogenic and biological components, with a focus on fish biophonies. Within the MPA, three sites were chosen each in a different protection zone (A for the integral protection, B as the partial protection, and C as the general protection). In each site, two underwater autonomous acoustic recorders were deployed in July 2020 at a depth of about 10 m on rocky bottoms. To characterise the contribution of both biophonies and anthrophonies, sea ambient noise (SAN) levels were measured as sound pressure level (SPL dB re: 1 μ Pa-rms) at eight 1/3 octave bands, centred from 125 Hz to 16 kHz, and biological and anthropogenic sounds were noted. Fish sounds were classified and counted following a catalogue of known fish sounds from the Mediterranean Sea based on the acoustic characteristic of sound types. A contemporary fish visual census had been carried out at the test sites. SPL were different by site, time (day . night), and hour. SPLs bands centred at 125, 250, and 500 Hz were significantly higher in the daytime, due to the high number of boats per minute whose noise dominated the soundscapes. The loudest man-made noise was found in the A zone, followed by the B and the C zone, confirming that MPA current regulations do not provide protection from acoustic pollution. The dominant biological components of the MPA soundscape were the impulsive sounds generated by some invertebrates, snapping shrimps and fish. The vast majority of fish sounds were recorded at the MPA site characterized by the highest sound richness, abundance, and Shannon-Wiener index, coherently with the results of a fish visual census. Moreover, the acoustic monitoring detected a sound associated with a cryptic species ( spp.) never reported in the study area before, further demonstrating the usefulness of passive acoustic monitoring as a complementary technique to species census. This study provides baseline data to detect future changes of the marine soundscapes and some suggestions to reduce the impact of noise on marine biodiversity.

In a wide variety of habitats, including some heavily urbanised areas, the adaptability of populations of common bottlenose dolphin ( Tursiops truncatus ) may depend on the social structure dynamics. Nonetheless, the way in which these adaptations take place is still poorly understood. In the present study we applied photo-identification techniques to investigate the social structure of the common bottlenose dolphin population inhabiting the Gulf of Alghero (Sardinia, Italy), analysing data recorded from 2008 to 2019. The social structure analysis showed a division of the entire population into five different communities and the presence of non-random associations, while there was no evidence of segregation between sexes. Furthermore, results highlighted an important change in social structure through time, likely due to a reduction in fish farm activity since 2015. The division of the population into different communities, the presence of segregation based on the foraging strategy (inside or outside the fish farm area) and the social network measures were evaluated by analysing independently the two datasets: the intense and low farm activity periods: 2008–2014 and 2015–2020, respectively. Segregation among individuals belonging to the same foraging strategy class was found only in the earlier period, and the composition of the four communities was consistent with this result. Our study improves the knowledge about bottlenose dolphin adaptation, as a lower complexity in social structure was linked to a reduction in anthropogenic food availability.

Indicators for preserving marine biodiversity include knowledge of how the spatial distribution and critical habitats of species overlap with human activities and impacts. Such indicators are key tools for marine spatial planning, a process that identifies and resolves conflicts between human uses and the conservation of marine environments. The common bottlenose dolphin in the Mediterranean Sea is considered a vulnerable species by the IUCN Red List and a priority species of the EU Habitat Directive. Here, we estimated spatio-temporal patterns of the species occurrence in the area around one Marine Protected Area (MPA) and two Sites of Community Importance (SCI) of the North western Sardinia, with the aim to predict the species distribution and the main links with the environmental factors and boat traffic. To evaluate whether dolphin groups with calves showed any habitat preference different from groups without calves, separate models for both type of groups were done. The most important contributing variables to the dolphin habitat suitability models were the likelihood of boat presence, habitat type and mean sea surface temperature. Different model outputs were obtained depending on dolphin group composition. The area of high likelihood of dolphin presence ranged between 30 and 60 km2 and was smaller for groups with calves. Further, the area of highest dolphin habitat suitability overlaps with the area of high boat traffic, suggesting that boating in the study site is a potential relevant anthropogenic threat to dolphins. Particularly, boating is concentrated inside and around the MPA/SCIs, indicating the need for stronger restriction measures. We propose updated SCI boundaries for effective protection of common bottlenose dolphins. These areas and the suggestions of regulation are specifically aimed at reducing the impact of boating on dolphins, especially for groups with calves. Synthesis and applications. Management measures should be designed based on the data here provided, and then implemented and enforced to decrease dolphin-boat interactions, especially for mother-calf pairs. The creation of new coastal SCIs should be considered especially where boat traffic overlaps with areas most suitable for dolphins. In these SCIs, boating should be managed to limit disturbance, avoidance or alterations of dolphin vital behaviour.

Background: Animal-assisted activities (AAA) are participative interventions, designed to lower hospitalization-related stress and anxiety, enhance communicative readiness, improve quality of life and encourage human–animal interaction. The aim of the present study was to evaluate AAA effects in the context of intensive rehabilitation of patients with spinal cord injury (SCI), traumatic brain injury (TBI), stroke. Methods: AAA in this study were structured by a local specialized association, for small groups of patients (5/7 a time), biweekly; each session lasted 60 min. Each patient participated in 10 sessions of AAA. Evaluation rating scales were administered at T0 (before the first session) and T1 (after the last session, five weeks later) as follows: Neurobehavioral Rating Scale (NRS) in case of patient with stroke/TBI without disorder of consciousness; Hospital Anxiety and Depression Scale (HADS) for SCI patients. Results: A total of 50 patients concluded the study. NRS score for joined TBI and stroke populations varied from a T0 mean value of 32.34 [C.I. 26.83–37.35] to 17.21 [C.I. 12.66–21.76] (46.7%); this difference proved to be statistically significant (p = 0.000). Stroke patients had a 57.6% NRS lowering by mean 28.10 [C.I. 20.55–35.65] points to 12 [C.I. 6.6–17.36], which was significant (p = 0.000); similarly, TBI patients showed a mean decrease of 35.8% points from the initial 41.6 points [C.I. 37.29–45.93] to 26.76 [C.I. 21.94–31.59] (p = 0.002). As for HADS scores a smaller improvement was found in the cohort of SCI patients: anxiety registered a 1 mean point decrease at T1 (21.5%), from the initial 6.5 points [C.I. 3.80–9.34] to 5.1 ones [C.I. 3.17–7.11]. This variation was near the threshold of significance (p = 0.05). Depression domain, instead, improved by 2.35 mean points (37%), from the 6.35 initial points [C.I. 3.45–9.26] to the final 4 [C.I. 2.15–5.98] with reaching of a significant p value (p = 0.03). ANCOVA did not confirm this last value and showed no influence of age and gender on outcome variations. Discussion: AAA showed preliminary evidence to decrease neurobehavioral disorders in patients with high-complexity neurological diseases, particularly stroke and TBI. The role of AAA in SCI patients remains unclear. Future studies should address confounders’ role for these populations, particularly severity of disease. Furthermore, AAA interventions will have to be studied on larger samples, deepening the exact phase to introduce AAA for neurological patients. Lastly, qualitative studies are needed to explore patients’ lived experiences.

Curcumin is a natural active principle with antioxidant, antibacterial and anti-inflammatory properties. Its use is limited by a low water solubility and fast degradation rate, which hinder its bioavailability. To overcome this problem, curcumin can be delivered through a carrier, which protects the drug molecule and enhances its pharmacological effects. The present work proposes a simple one-pot sol–gel synthesis to obtain a hybrid carrier for curcumin delivery. The hybrid consists of a mesostructured matrix of amorphous silica, which stabilizes the carrier, and hexadecyltrimethylammonium (CTA), a surfactant where curcumin is dissolved to increase its water solubility. The carrier was characterized in terms of morphology (FESEM), physicochemical properties (XRD, FTIR, UV spectroscopy) and release capability in pseudo-physiological solutions. Results show that curcumin molecules were entrapped, for the first time, in a silica-surfactant mesostructured hybrid carrier. The hybrid carrier successfully released curcumin in artificial sweat and in a phosphate buffer saline solution, so confirming its efficacy in increasing curcumin water solubility. The proposed drug release mechanism relies on the degradation of the carrier, which involves the concurrent release of silicon. This suggests strong potentialities for topical administration applications, since curcumin is effective against many dermal diseases while silicon is beneficial to the skin. A simple one-pot sol–gel organic-solvent-free synthesis, at room temperature, leads to the formation of a hybrid mesostructured CTA–silica containing curcumin. When in pseudo-physiological solutions, the hybrid releases curcumin (enclosed in surfactant micelles), with enhanced solubility, and silicates. This material is a promising candidate for topical administration of drugs, since curcumin is effective against several dermatological diseases and silicon is beneficial to skin. Highlights A novel mesostructured curcumin-containing hybrid carrier was successfully synthesized. The hybrid enhances curcumin solubility in pseudo-physiological conditions (e.g., artificial sweat). Curcumin release is governed by a degradation process of the silica matrix. Degradation of the hybrid induces the simultaneous release of curcumin, silicates, and surfactant.

(1) Background: The use of N-acetylcysteine (NAC) to relieve meconium obstruction of prematurity in the first days of life has been reported, with NAC reducing the viscosity of luminal contents by cleaving the disulfide bonds of mucoproteins. However, its use in this population should be further explored since it has been associated with hypernatremia and transient increase in transaminases and bilirubin. (2) Methods: In this retrospective study, we included neonates admitted because of enteral feeding intolerance and intestinal obstruction from 2019 to 2021 who received NAC as a rescue therapy before explorative laparotomy. (3) Results: We summarized the clinical presentation of six preterm neonates with enteral feeding intolerance and intestinal obstruction who received NAC as a rescue therapy. Four infants (66.7%) gradually improved without the need for explorative laparotomy, whereas two infants (33.3%) underwent the creation of an ileostomy. No cases of hypernatremia or hepatic derangement associated with NAC therapy were observed. (4) Conclusions: We described the use of NAC treatment by nasogastric tube and/or rectal enemas in preterm infants with enteral feeding intolerance and intestinal obstruction after a multidisciplinary assessment, but the limited sample size did not allow us to obtain definitive conclusions and further research is needed in this field, given the limited evidence about NAC treatment in preterm infants.

Acute vertigo is defined as the perception of movement of oneself or the surroundings in the absence of actual motion and it is a frequent cause for emergency department admissions. The utilization of medical resources and the duration of hospital stay for this kind of symptom is high. Furthermore, the efficiency of brain imaging in the acute phase is low, considering the limited sensitivity of both CT and MRI for diagnosing diseases that are the causes of central type of vertigo. Relying on imaging tests can provide false reassurance in the event of negative results or prolong the in-hospital work-up improperly. On the other hand, clinical examinations, notably the assessment of nystagmus’ features, have proven to be highly accurate and efficient when performed by experts. Literature data point out that emergency physicians often do not employ these skills or use them incorrectly. Several clinical algorithms have been introduced in recent years with the aim of enhancing the diagnostic accuracy of emergency physicians when evaluating this specific pathology. Both the ‘HINTS and ‘STANDING’ algorithms have undergone external validation in emergency physician hands, showing good diagnostic accuracy. The objective of this consensus document is to provide scientific evidence supporting the clinical decisions made by physicians assessing adult patients with acute vertigo in the emergency department, particularly in cases without clear associated neurological signs. The document aims to offer a straightforward and multidisciplinary approach. At the same time, it tries to delineate benchmarks for the formulation of local diagnostic and therapeutic pathways, as well as provide a base for the development of training and research initiatives.