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We investigate non-Hermitian elastic lattices characterized by non-local feedback interactions. In one-dimensional lattices, proportional feedback produces non-reciprocity associated with complex dispersion relations characterized by gain and loss in opposite propagation directions. For non-local controls, such non-reciprocity occurs over multiple frequency bands characterized by opposite non-reciprocal behavior. The dispersion topology is investigated with focus on winding numbers and non-Hermitian skin effect, which manifests itself through bulk modes localized at the boundaries of finite lattices. In two-dimensional lattices, non-reciprocity is associated with directional wave amplification. Moreover, the combination of skin effect in two directions produces modes that are localized at the corners of finite two-dimensional lattices. Our results describe fundamental properties of non-Hermitian elastic lattices, and suggest new possibilities for the design of meta materials with novel functionalities related to selective wave filtering, amplification and localization. The considered non-local lattices also provide a platform for the investigation of topological phases of non-Hermitian systems.

We investigate a family of quasiperiodic continuous elastic beams, the topological properties of their vibrational spectra, and their relation to the existence of localized modes. We specifically consider beams featuring arrays of ground springs at locations determined by projecting from a circle onto an underlying periodic system. A family of periodic and quasiperiodic structures is obtained by smoothly varying a parameter defining such projection. Numerical simulations show the existence of vibration modes that first localize at a boundary, and then migrate into the bulk as the projection parameter is varied. Explicit expressions predicting the change in the density of states of the bulk define topological invariants that quantify the number of modes spanning a gap of a finite structure. We further demonstrate how modulating the phase of the ground springs distribution causes the topological states to undergo an edge-to-edge transition. The considered configurations and topological studies provide a framework for inducing localized modes in continuous elastic structural components through globally spanning, deterministic perturbations of periodic patterns defined by the considered projection operations.

Numerous archaeal lineages are known to inhabit marine subsurface sediments, although their distributions, metabolic capacities, and interspecies interactions are still not well understood. Abundant and diverse archaea were recently reported in Costa Rica (CR) margin subseafloor sediments recovered during IODP Expedition 334. Here, we recover metagenome-assembled genomes (MAGs) of archaea from the CR margin and compare them to their relatives from shallower settings. We describe 31 MAGs of six different archaeal lineages (Lokiarchaeota, Thorarchaeota, Heimdallarchaeota, Bathyarcheota, Thermoplasmatales, and Hadesarchaea) and thoroughly analyze representative MAGs from the phyla Lokiarchaeota and Bathyarchaeota. Our analysis suggests the potential capability of Lokiarchaeota members to anaerobically degrade aliphatic and aromatic hydrocarbons. We show it is genetically possible and energetically feasible for Lokiarchaeota to degrade benzoate if they associate with organisms using nitrate, nitrite, and sulfite as electron acceptors, which suggests a possibility of syntrophic relationships between Lokiarchaeota and nitrite and sulfite reducing bacteria. The novel Bathyarchaeota lineage possesses an incomplete methanogenesis pathway lacking the methyl coenzyme M reductase complex and encodes a noncanonical acetogenic pathway potentially coupling methylotrophy to acetogenesis via the methyl branch of Wood–Ljungdahl pathway. These metabolic characteristics suggest the potential of this Bathyarchaeota lineage to be a transition between methanogenic and acetogenic Bathyarchaeota lineages. This work expands our knowledge about the metabolic functional repertoire of marine benthic archaea.

Most sensory information destined for the neocortex is relayed through the thalamus, where considerable transformation occurs 1 , 2 . One means of transformation involves interactions between excitatory thalamocortical neurons that carry data to the cortex and inhibitory neurons of the thalamic reticular nucleus (TRN) that regulate the flow of those data 3 – 6 . Although the importance of the TRN has long been recognised 7 – 9 , understanding of its cell types, their organization and their functional properties has lagged behind that of the thalamocortical systems they control. Here we address this by investigating the somatosensory and visual circuits of the TRN in mice. In the somatosensory TRN we observed two groups of genetically defined neurons that are topographically segregated and physiologically distinct, and that connect reciprocally with independent thalamocortical nuclei through dynamically divergent synapses. Calbindin-expressing cells—located in the central core—connect with the ventral posterior nucleus, the primary somatosensory thalamocortical relay. By contrast, somatostatin-expressing cells—which reside along the surrounding edges of the TRN—synapse with the posterior medial thalamic nucleus, a higher-order structure that carries both top-down and bottom-up information 10 – 12 . The two TRN cell groups process their inputs in pathway-specific ways. Synapses from the ventral posterior nucleus to central TRN cells transmit rapid excitatory currents that depress deeply during repetitive activity, driving phasic spike output. Synapses from the posterior medial thalamic nucleus to edge TRN cells evoke slower, less depressing excitatory currents that drive more persistent spiking. Differences in the intrinsic physiology of TRN cell types, including state-dependent bursting, contribute to these output dynamics. The processing specializations of these two somatosensory TRN subcircuits therefore appear to be tuned to the signals they carry—a primary central subcircuit tuned to discrete sensory events, and a higher-order edge subcircuit tuned to temporally distributed signals integrated from multiple sources. The structure and function of visual TRN subcircuits closely resemble those of the somatosensory TRN. These results provide insights into how subnetworks of TRN neurons may differentially process distinct classes of thalamic information. In the thalamic reticular nucleus there are two neuron types that are segregated into central and edge zones and receive inputs from different thalamocortical nuclei, creating subcircuits with distinct dynamics.

Dinoflagellates are haploid eukaryotic microalgae in which rapid proliferation causes dense blooms, with harmful health and economic effects to humans. The proliferation mode is mainly asexual, as the sexual cycle is believed to be rare and restricted to stressful environmental conditions. However, sexuality is key to explaining the recurrence of many dinoflagellate blooms because in many species the fate of the planktonic zygotes (planozygotes) is the formation of resistant cysts in the seabed (encystment). Nevertheless, recent research has shown that individually isolated planozygotes in the lab can enter other routes besides encystment, a behavior of which the relevance has not been explored at the population level. In this study, using imaging flow cytometry, cell sorting, and Fluorescence In Situ Hybridization (FISH), we followed DNA content and nuclear changes in a population of the toxic dinoflagellate Alexandrium minutum that was induced to encystment. Our results first show that planozygotes behave like a population with an \"encystment-independent\" division cycle, which is light-controlled and follows the same Light:Dark (L:D) pattern as the cycle governing the haploid mitosis. Resting cyst formation was the fate of just a small fraction of the planozygotes formed and was restricted to a period of strongly limited nutrient conditions. The diploid-haploid turnover between L:D cycles was consistent with two-step meiosis. However, the diel and morphological division pattern of the planozygote division also suggests mitosis, which would imply that this species is not haplontic, as previously considered, but biphasic, because individuals could undergo mitotic divisions in both the sexual (diploid) and the asexual (haploid) phases. We also report incomplete genome duplication processes. Our work calls for a reconsideration of the dogma of rare sex in dinoflagellates.

We investigate the spectral properties of one-dimensional spatially modulated nonlinear phononic lattices, and their evolution as a function of amplitude. In the linear regime, the stiffness modulations define a family of periodic and quasiperiodic lattices whose bandgaps host topological edge states localized at the boundaries of finite domains. With cubic nonlinearities, we show that edge states whose eigenvalue branch remains within the gap as amplitude increases remain localized, and therefore appear to be robust with respect to amplitude. In contrast, edge states whose corresponding branch approaches the bulk bands experience de-localization transitions. These transitions are predicted through continuation studies on the linear eigenmodes as a function of amplitude, and are confirmed by direct time domain simulations on finite lattices. Through our predictions, we also observe a series of amplitude-induced localization transitions as the bulk modes detach from the nonlinear bulk bands and become discrete breathers that are localized in one or more regions of the domain. Remarkably, the predicted transitions are independent of the size of the finite lattice, and exist for both periodic and quasiperiodic lattices. These results highlight the co-existence of topological edge states and discrete breathers in nonlinear modulated lattices. Their interplay may be exploited for amplitude-induced eigenstate transitions, for the assessment of the robustness of localized states, and as a strategy to induce discrete breathers through amplitude tuning.

Oil reservoirs have been shown to house numerous microbial lineages that differ based on the in-situ pH, salinity and temperature of the subsurface environment. Lineages of Firmicutes, including Clostridiales, have been frequently detected in oil reservoirs, but are typically not considered impactful or relevant due to their spore-forming nature. Here we show, using metagenomics, a high temperature oil reservoir of marine salinity contains a microbial population that is predominantly from within the Order Clostridiales. These organisms form an oil-reservoir specific clade based on the phylogenies of both 16S rRNA genes and ribosomal proteins, which we propose to name U Petromonas tenebris , meaning they are single-celled organisms from dark rocks. Metagenome-assembled genomes (MAGs) of these Petromonas sp. were obtained and used to determine that these populations, while capable of spore-formation, were also likely replicating in situ in the reservoir. We compared these MAGs to closely related genomes and show that these subsurface Clostridiales differ, from the surface derived genomes, showing signatures of the ability to degrade plant-related compounds, whereas subsurface genomes only show the ability to process simple sugars. The estimation of in-situ replication from genomic data suggest that U Petromonas tenebris lineages are functional in-situ and may be specifically adapted to inhabit oil reservoirs.

Mitochondrial topoisomerase IB (TOP1MT) is a nuclear-encoded topoisomerase, exclusively localized to mitochondria, which resolves topological stress generated during mtDNA replication and transcription. Here, we report that TOP1MT is overexpressed in cancer tissues and demonstrate that TOP1MT deficiency attenuates tumor growth in human and mouse models of colon and liver cancer. Due to their mitochondrial dysfunction, TOP1MT-KO cells become addicted to glycolysis, which limits synthetic building blocks and energy supply required for the proliferation of cancer cells in a nutrient-deprived tumor microenvironment. Mechanistically, we show that TOP1MT associates with mitoribosomal subunits, ensuring optimal mitochondrial translation and assembly of oxidative phosphorylation complexes that are critical for sustaining tumor growth. The TOP1MT genomic signature profile, based on Top1mt -KO liver cancers, is correlated with enhanced survival of hepatocellular carcinoma patients. Our results highlight the importance of TOP1MT for tumor development, providing a potential rationale to develop TOP1MT-targeted drugs as anticancer therapies. TOP1MT is a topoisomerase that is localised to mitochondria. Here, the authors show that TOP1MT has a tumor promoting role in hepatocellular carcinoma by supporting mitochondrial translation and that its deficiency limits tumorigenicity.

Bacteriophages play significant roles in the composition, diversity, and evolution of bacterial communities. Despite their importance, it remains unclear how phage diversity and phage-host interactions are spatially structured. Local adaptation may play a key role. Nitrogen-fixing symbiotic bacteria, known as rhizobia, have been shown to locally adapt to domesticated common bean at its Mesoamerican and Andean sites of origin. This may affect phage-rhizobium interactions. However, knowledge about the diversity and coevolution of phages with their respective Rhizobium populations is lacking. Here, through the study of four phage-Rhizobium communities in Mexico and Argentina, we show that both phage and host diversity is spatially structured. Cross-infection experiments demonstrated that phage infection rates were higher overall in sympatric rhizobia than in allopatric rhizobia except for one Argentinean community, indicating phage local adaptation and host maladaptation. Phage-host interactions were shaped by the genetic identity and geographic origin of both the phage and the host. The phages ranged from specialists to generalists, revealing a nested network of interactions. Our results suggest a key role of local adaptation to resident host bacterial communities in shaping the phage genetic and phenotypic composition, following a similar spatial pattern of diversity and coevolution to that in the host.

We investigate the dynamic behavior of lattices with disorder introduced through non-local network connections. Inspired by the Watts–Strogatz small-world model, we employ a single parameter to determine the probability of local connections being re-wired, and to induce transitions between regular and disordered lattices. These connections are added as non-local springs to underlying periodic one-dimensional (1D) and two-dimensional (2D) square, triangular and hexagonal lattices. Eigenmode computations illustrate the emergence of spectral gaps in various representative lattices for increasing degrees of disorder. These gaps manifest themselves as frequency ranges where the modal density goes to zero, or that are populated only by localized modes. In both cases, we observe low transmission levels of vibrations across the lattice. Overall, we find that these gaps are more pronounced for lattice topologies with lower connectivity, such as the 1D lattice or the 2D hexagonal lattice. We then illustrate that the disordered lattices undergo transitions from ballistic to super-diffusive or diffusive transport for increasing levels of disorder. These properties, illustrated through numerical simulations, unveil the potential for disorder in the form of non-local connections to enable additional functionalities for metamaterials. These include the occurrence of disorder-induced spectral gaps, which is relevant to frequency filtering devices, as well as the possibility to induce diffusive-type transport which does not occur in regular periodic materials, and that may find applications in dynamic stress mitigation.