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Microorganisms, including pathogenic or opportunistic bacteria and fungi, may gain access to the uterus during breeding, and infectious endometritis plays a major role in equine subfertility. This study aimed to assess the post-breeding inflammatory response, endometrial culture, and embryo recovery of mares susceptible to persistent breeding-induced endometritis (PBIE) treated with plasma-rich (PRP) or -poor (PPP) plasma. Mares (n = 12) susceptible to PBIE had three cycles randomly assigned to receive intrauterine infusions of lactate ringer solution (LRS, control), or autologous PRP or PPP pre- (−48 and −24 h) and post-breeding (6 and 24 h). Mares were bred with fresh semen from one stallion. Intrauterine fluid accumulation (IUF) and endometrial neutrophils were assessed every 24 h up to 96 h post-breeding. Uterine cytokines (Ilβ, IL6, CXCL8, and IL10) were evaluated before (0 h), 6, and 24 h post-breeding, and endometrial culture three and nine days after breed. Embryo flushing was performed 8 days post-ovulation. Data were analyzed with mixed model, Tukey’s post-hoc test, and multivariate regression. PRP treatment reduced endometrial neutrophils, post-breeding IUF, and pro-inflammatory cytokines when compared to control-assigned cycles, but not significantly different than PPP. Controls had a significantly higher percentage of positive bacterial cultures (33%) in comparison to PRP-assigned cycles (0%), whereas cycles treated with PPP were not significantly different from the other groups (25%). The PRP-assigned cycles had significantly greater embryo recovery rates (83%) than the control (33%), though not significantly different than PPP (60%). Plasma infusion reduced the duration and intensity of the post-breeding inflammatory response and improved embryo recovery in mares susceptible to PBIE. Platelets incrementally downregulate PBIE and appear to have a dose-dependent antimicrobial property.

In light of PRP’s increasing popularity in veterinary practice, this study aimed to compare three manual methods to prepare and cool equine PRP. The blood of 18 clinically healthy mares was collected via venipuncture in a blood transfusion bag (method 1), blood tubes (method 2), and a syringe (method 3). In method 1, samples were double centrifuged; method 2 involved one centrifugation, and in method 3 the syringe was kept in an upright position to sediment for 4 h. After processing with three methods, PRP and platelet-poor plasma (PPP) were extracted and assessed for red (RBC) and white blood cell counts (WBC), platelet counts, and viability. In a subset of mares (n = 6), samples were processed with the three methods, and PRP was evaluated at 6 and 24 h postcooling at 5 °C. Method 1 resulted in the highest and method 3 in the lowest platelet concentration (p < 0.05), and the latter also had greater contamination with WBC than the others (p < 0.001). Platelet viability was similar across treatments (p > 0.05). Cooling for 24 h did not affect platelet counts in all methods (p > 0.05); however, platelet viability was reduced after cooling PRP produced by method 3 (p = 0.04), and agglutination increased over time in all methods (p < 0.001). The three methods increased (1.8–5.6-fold) platelet concentration in PRP compared to whole blood without compromising platelet viability. In conclusion, all three methods concentrated platelets and while cooling affected their viability. It remains unknown whether the different methods and cooling would affect PRP’s clinical efficacy.

A 5-yr-old, intact male red ruffed lemur (Varecia rubra) presented for evaluation as the result of a 1-wk history of lethargy and hyporexia. Physical examination findings included thin body condition, muffled heart sounds, harsh lung sounds, and liquid brown diarrhea. Complete blood count and serum biochemistry showed an inflammatory leukogram, mild hyponatremia, and mild hypochloremia. Orthogonal trunk radiographs revealed a severe alveolar pattern in the right cranial lung lobes with cardiac silhouette effacement. Thoracic ultrasound confirmed a large, hypoechoic mass in the right lung lobes. Fine-needle aspiration of the lung mass and cytology revealed fungal yeast organisms, consistent with Blastomyces dermatitidis. Blastomyces Quantitative EIA Test on urine was positive. Postmortem examination confirmed systemic blastomycosis involving the lung, tracheobronchial lymph nodes, spleen, kidney, liver, cerebrum, and eye. To the authors' knowledge, this is the first report of blastomycosis in a prosimian species.

Topic: paediatric trauma Outcome rating: worth a peek This study aimed to use machine learning to identify the hospital, ED and patient variables affecting survival among paediatric (≤18 years old) trauma patients using data submitted to the US National Trauma Data Bank (NTDB) for the years 2012–2017.2 A total of 274 756 injured children were included from 458 trauma centres, excluding those centres with no deaths and those who saw <50 patients in 6 years. [...]at the ED level, variables showing association with survival were policy for mental health (+8.8% change in survival), policy on patient assessment/reassessment (+7.5%), specific respiratory equipment (+7.2%), policy on reduced-dose radiation (+7%), physician competency evaluations (+4.9%), measuring weight in kilogram (+3.2%), nursing team completing life support courses (+2.5%) and validated paediatric triage tool (+2.5%). Intranasal fentanyl and discharge from the emergency department among children with sickle cell disease and vaso-occlusive pain: a multicentre paediatric emergency medicine perspective by Rees et al. Hospital mortality was 6.5% in both groups, but COVID-19-positive patients spent longer on a ventilator, had longer intensive care and hospital length of stays, and had higher rates of myocardial infarction and cardiac arrest.

The Day and Ryusuke Hamaguchi’s Evil Does Not Exist lead the nominations for the 17th Asian Film Awards, with six nods each including best film. Scroll down for full list of nominations The winners will be announced at a ceremony in Hong Kong on March 10 and will be decided by a jury led by cult Japanese filmmaker Kiyoshi Kurosawa. Securing four nominations was Snow Leopard, which won best film at Toyko International Film Festival following the death of Tibetan-Chinese filmmaker Pema Tseden last year. [...]multiple nominees included Hirokazu Kore-Eda’s Monster, big-budget Hong Kong feature The Goldfinger and Japanese monster hit Godzilla Minus One. Asian Film Awards 2024 Nominations Best Film 12.12:

“For Armenia to get a nomination would be life changing for the country,” says Serj Tankian, an executive producer on the film who is best known as the Grammy-winning frontman of System of a Down. Tankian has been on board since reading the first draft of the script, after being introduced to Goorjian by US filmmaker Garin Hovannisian, the director of documentary Truth To Power that explored the heavy metal musician’s influence on political revolutionaries in Armenia. Nominations voting runs January 11-16 and nominations will be announced on January 23.

The Fruit is based on the filmmaker’s own personal experience and follows a woman’s journey through the joy of pregnancy and sadness of a miscarriage. The industry screenings run parallel to the Festival of Young Cinema, which runs January 5-11 and aims to serve audiences from Macau, Hong Kong and mainland China. Elaine HUANG When The Bottle TurnsDir.

The role of the flamboyant chairman Ching proved a welcome challenge to Leung, whose recent films include Marvel superhero title Shang-Chi And The Legend Of The Ten Rings, Hong Kong crime drama Where The Wind Blows and Chinese spy thriller Hidden Blade. “For me, every scene playing this role was challenging because I have never played someone this cocky or unreserved,” says Leung. “Recent Hong Kong films focus more on local social issues and topics. Because the market is shrinking, we have fewer productions. “Is it still possible for Daniel Day-Lewis to play my role?,” adds Lau with a laugh. * ‘The Goldfinger’: Review