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In recent years, the essential roles of digital payment have gradually emerged. However, current research on digital payment adoption models rarely incorporates the outcomes of digital payment, and it also gives less consideration to rural residents. Considering these two issues as a research gap, this article establishes a unified digital payment use and credit availability model by extending the UTAUT2 framework in two aspects and applying it to China’s rural residents. The first extension is to add credit availability as an outcome variable of digital payment use. The second is to add two factors important to farmers, perceived riskiness and innovativeness, as constructs. Structural equation modeling is employed to analyze data collected from nearly 500 Chinese rural residents. The results show that almost 90% of rural residents have used digital payment. However, only a low proportion use it for many purposes or frequently. Rural residents’ digital payment use can increase credit availability. Performance expectancy, effort expectancy, social influence, price value, and innovativeness significantly and positively affect digital payment intention and behavior, thereby indirectly improving credit availability. However, the perceived risk does not influence digital payment adoption, possibly due to effective protective behaviors. In addition to the indirect effect, the results show that innovativeness also has a direct impact on credit availability. JEL Classification: M15, G21.

Background Ubiquitous CCCH nucleic acid-binding motif is found in a wide-variety of organisms. CCCH genes are involved in plant developmental processes and biotic and abiotic stress responses. Brassica rapa is a vital economic crop and classical model plant of polyploidy evolution, but the functions of CCCH genes in B. rapa are unclear. Results In this study, 103 CCCH genes in B. rapa were identified. A comparative analysis of the chromosomal position, gene structure, domain organization and duplication event between B. rapa and Arabidopsis thaliana were performed. Results showed that CCCH genes could be divided into 18 subfamilies, and segmental duplication might mainly contribute to this family expansion. C-X 7/8 -C-X 5 -C 3 -H was the most commonly found motif, but some novel CCCH motifs were also found, along with some loses of typical CCCH motifs widespread in other plant species. The multifarious gene structures and domain organizations implicated functional diversity of CCCH genes in B. rapa . Evidence also suggested functional redundancy in at least one subfamily due to high conservation between members. Finally, the expression profiles of subfamily-IX genes indicated that they are likely involved in various stress responses. Conclusion This study provides the first genome-wide characterization of the CCCH genes in B. rapa . The results suggest that B. rapa CCCH genes are likely functionally divergent, but mostly involved in plant development and stress response. These results are expected to facilitate future functional characterization of this potential RNA-binding protein family in Brassica crops.

Rapeseed (Brassica napus) is an essential oil resource, but its yield can be significantly compromised by Sclerotinia sclerotiorum (S. sclerotiorum) infection. Due to the absence of rapeseed strains that are highly or completely immune to S. sclerotiorum, enhancing rapeseed resistance through genetic approaches is challenging. In this study, we developed a novel method to enhance rapeseed resistance to S. sclerotiorum using β-ocimene. Our results demonstrated that β-ocimene treatment significantly strengthened the defense capabilities of rapeseed. β-ocimene treatment can simultaneously activate multiple defense-related signaling pathways, including jasmonic acid signaling, salicylic acid signaling, and MAPK signaling, in rapeseed, while also inducing the accumulation of secondary metabolites coniferyl aldehyde—a key secondary metabolite in the phenylpropanoid pathway critical for plant defense responses. Furthermore, applying coniferyl aldehyde to the leaves of rapeseed can remarkably enhance its resistance to sclerotinia disease. Collectively, these findings confirm that β-ocimene activates the defense system of rapeseed, elevates the content of coniferyl aldehyde, and thereby enables rapeseed to effectively combat sclerotinia disease. The metabolomics data are available via MetaboLights under the identifier MTBLS12510. In conclusion, this study not only uncovers the mechanism by which β-ocimene induces rapeseed resistance to sclerotinia disease but also presents a novel approach for its prevention and control.

Objective To compare recurrence and survival in patients with stage III endometrial cancer after radical surgery, followed by either adjuvant chemoradiotherapy (ACR) or adjuvant chemotherapy (AC). Methods We searched for relevant studies in PubMed Central, Embase and the Cochrane Central Register of Controlled Trials. Data were pooled on rates of recurrence as well as rates of progression-free, disease-free and overall survival. Heterogeneity was evaluated using the I 2 test. Subgroup and sensitivity analyses were performed to identify potential sources of heterogeneity. Results Data from 18,375 patients in 15 retrospective studies and one randomized controlled trial were meta-analyzed. Compared to the AC group, the ACR showed significantly lower risk of local recurrence (OR 0.43, 95%CI 0.32–0.59) and total recurrence (OR 0.72, 95%CI 0.58–0.89). ACR was also associated with significantly better overall survival (HR 0.66, 95%CI 0.57–0.76), progression-free survival (HR 0.56, 95%CI 0.39–0.81) and disease-free survival (HR 0.66, 95%CI 0.53–0.83). Conclusions Adding adjuvant radiotherapy to adjuvant chemotherapy after radical surgery may significantly reduce risk of local and overall recurrence, while significantly improving survival of patients with stage III endometrial cancer.

Both the microgravity solidification mechanism and resultant micromechanical properties of ternary Ni41Fe40Ti19 alloy were investigated by means of drop tube, nanoindentation, and nano-dynamic mechanical analysis (Nano-DMA) techniques. The microstructure of the Ni41Fe40Ti19 alloy droplets consisted of γ-(Fe,Ni) dendrites and interdendritic pseudobinary eutectic phases. The average cooling rate and undercooling increased significantly as the droplet diameter decreased during free fall. Owing to the refinement of the rapidly solidified microstructure, and the Ti solute hardening of the primary γ-(Fe,Ni) dendrites, the microhardness of this alloy was remarkably increased with the decrease of droplet size. Moreover, the nanohardness of the γ-(Fe,Ni) dendrite increased as the indentation displacement decreased within the depth range of 40 to 244 nm, indicating a conspicuous indentation size effect (ISE). However, the ISE increased as the undercooling increased, because additional geometrically necessary dislocations (GNDs) were required, while intragranular dislocation motion was further hindered as the Ti content increased. The size effect factor increased linearly with the reduced droplet diameter.

AbstractIntroduction: Limited treatment options are available for residual locally advanced cervical cancer after concurrent radio-chemotherapy. Pembrolizumab has been approved by the US Food and Drug Administration to treat patients with persistent, recurrent, and metastatic cervical cancer. However, it is expensive and not covered by medical insurance in China. Case Description: A patient presented with stage IIIC1 cervical cancer characterized by PTEN, PIK3CA, MTOR, and ARID1A mutations, accompanied by low programmed cell death-ligand 1 expression (tumor cell proportion score 1% and combined positive score 1). The patient exhibited a residual cervical lesion after concurrent radio-chemotherapy but eventually achieved a pathologically complete response through a combination of the programmed cell death-1 (PD-1) inhibitor camrelizumab with the antiangiogenic drug apatinib (two medicines produced by Chinese Jiangsu Hengrui Pharmaceutical Co.). Surgical intervention confirmed the lack of residual tumor cells in the cervix. Regular follow-up confirmed that disease-free survival time was 41 months and overall survival time was 51 months. Adverse events, including cutaneous capillary endothelial proliferation, hepatic insufficiency, hemorrhoidal hemorrhage, and neutropenia, were manageable during treatment with camrelizumab and apatinib. Conclusion: Our findings suggest that the combination of camrelizumab and apatinib could offer a valuable therapeutic option for residual advanced cervical cancer patients after concurrent radio-chemotherapy. Camrelizumab is affordable, at just 10% of the price of pembrolizumab, although it is similarly not covered by medical insurance for cervical cancer in China. The PTEN, PIK3CA, MTOR, and ARID1A gene mutations hold the potential to serve as predictive biomarkers for cervical cancer patients treated by PD-1 inhibitors.

ObjectiveThe anti-malarial drug, artemisinin, is harvested from the leaves of adult Artemisia annua L. plants. As its concentration in juvenile plants is very low, the present study aimed to assess if the airborne signaling molecule, β-ocimene, could be used to enhance artemisinin accumulation in juvenile A. annua plants.ResultsApplication of exogenous β-ocimene increased artemisinin accumulation in A. annua. Treatment with 10 µM β-ocimene for 4 days resulted in juvenile plants accumulating artemisinin contents of up to 25 mg/g (2.5%) of dry weight. The expression levels of key genes encoding enzymes involved in both precursor biosynthetic pathways and artemisinin biosynthetic pathways induced by β-ocimene were upregulated. Glandular secretory trichome (GST) size and density increased by 49.2% and 38.2%, respectively, along with the upregulation of genes associated with GST development.Conclusionβ-ocimene enhances artemisinin accumulation in juvenile A. annua plants by modulating artemisinin biosynthetic pathways and GST development.

Understanding the evolutionary forces in speciation is a central goal in evolutionary biology. Asian cultivated rice has two subspecies, indica and japonica , but the underlying mechanism of the partial reproductive isolation between them remains obscure. Here we show a presence-absence variation (PAV) at the Se locus functions as an indica-japonica reproductive barrier by causing hybrid sterility (HS) in indica-japonica crosses. The locus comprises two adjacent genes: ORF3 encodes a sporophytic pollen killer, whereas ORF4 protects pollen in a gametophytic manner. In F 1 of indica-japonica crosses, pollen with the japonica haplotype, which lacks the sequence containing the protective ORF4 , is aborted due to the pollen-killing effect of ORF3 from indica . Evolutionary analysis suggests ORF3 is a gene associated with the Asian cultivated rice species complex, and the PAV has contributed to the reproductive isolation between the two subspecies of Asian cultivated rice. Our analyses provide perspectives on rice inter-subspecies post-zygotic isolation, and will promote efforts to overcome reproductive barriers in indica-japonica hybrid rice breeding. The mechanisms of the partial reproductive isolation between the two subspecies of rice remains obscure. Here, the authors show that the two adjacent genes form a killer-protector system to induce hybrid male sterility and reproductive isolation between indica and japonica .

Due to the complex environment of caged chicken coops, uneven illumination and severe occlusion in the coops lead to unsatisfactory accuracy of chicken detection. In this study, we construct an image dataset in the production environment of caged chickens using a head and neck co-annotation method and a multi-stage co-enhancement strategy, and we propose Chicken-YOLO, an occlusion-aware caged chicken detection model based on multi-scale edge information extractor and context fusion for the severe occlusion and poor illumination situations. The model enhances chicken feather texture and crown contour features via the multi-scale edge information extractor (MSEIExtractor), optimizes downsampling information retention through integrated context-guided downsampling (CGDown), and improves occlusion perception using the detection head with the multi-scale separation and enhancement attention module (DHMSEAM). Experiments demonstrate that Chicken-YOLO achieves the best detection performance among mainstream models, exhibiting 1.7% and 1.6% improvements in mAP50 and mAP50:95, respectively, over the baseline model YOLO11n. Moreover, the improved model achieves higher mAP50 than the superior YOLO11s while using only 58.8% of its parameters and 42.3% of its computational cost. On the two specialized test sets—one for poor illumination cases and the other for multiple occlusion cases—Chicken-YOLO’s performance improves significantly, with mAP50 increasing by 3.0% and 1.8%, respectively. This suggests that the model enhances target capture capability under poor illumination and maintains better contour continuity in occlusion cases, verifying its robustness against complex disturbances.

Sulfotransferases (SOTs; EC 2.8.2.-), which are widespread from prokaryotes to eukaryotes, constitute a multi-protein family that plays crucial roles in plant growth, development and stress adaptation. However, this family has not been systemically investigated in Brassica rapa. Here, a genome-wide systemic analysis of SOT genes in B. rapa subsp. pekinensis, a globally cultivated vegetable, were conducted. We identified 56 SOT genes from the whole B. rapa genome using Arabidopsis SOT sequences as queries and classified them into nine groups, rather than the eight groups of previous research. 56 B. rapa SOT genes (BraSOTs) were distributed on all 10 chromosomes except for chromosome 5. Of these, 27 BraSOTs were distributed in seven clusters on five chromosomes (ChrA01, ChrA02, Chr03, ChrA07, and Chr09). Among the BraSOT proteins, 48 had only one SOT_1 domain and 6 had two, while 2 had one SOT_3 domain. Additionally, 47 BraSOT proteins contained only known SOT domains. The remaining nine proteins, five in group-VIII and two in group-IX, contained additional transmembrane domains. Specific motif regions I and IV for 3'-phosphoadenosine 5'-phosphosulfate binding were found in 41 BraSOT proteins. Introns were present in only 18 BraSOT genes, and all seven BraSOT genes in groups VIII and IX had more than three introns. To identify crucial SOTs mediating the response to abiotic stress in B. rapa, expression changes in 56 BraSOT genes were determined by quantitative RT-PCR after drought, salinity, and ABA treatments, and some BraSOT genes were associated with NaCl, drought and ABA stress, e.g. Bra017370, Bra009300, Bra027880.