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This study comprehensively analyzed the heat loss and total heat transfer coefficient (U-value) of a single-span experimental plastic greenhouse covered with a double layer of 0.1 mm thick polyethylene. The air temperature and heat flux (W m−2) of the greenhouse components were measured from 18:00 to 06:00, and the energy balance equations under steady-state conditions were determined. The heat flux and U-value of the roof, sides, front and rear, and floor of the greenhouse were determined and compared. The results showed that these values for the roof play an important role in determining the heat load in the greenhouse, and that the average heat transfer through the floor is very small. The average U-value of the greenhouse cover is a comprehensive value which takes the U-values of the roof, sides, and front and rear into account through the use of an area–weighted average method. Finally, an average U-value of 3.69 W m−2 °C −1 was obtained through the analysis of the variations in the U-value, as it is related to the difference in air temperature between the interior and exterior of the greenhouse, as well as to the outdoor wind speed. The relationships between the average U-value and those of the roof, sides, and front and rear of the experimental greenhouse were modeled, and were shown to have a highly linear relationship.