Explore the vast range of titles available.

Azo dyes are one of the largest classes of synthetic dyes being used in textile industries. It has been reported that 15–50% of these dyes find their way into wastewater that is often used for irrigation purpose in developing countries. The effect of azo dyes contamination on soil nitrogen (N) has been studied previously. However, how does the azo dye contamination affect soil carbon (C) cycling is unknown. Therefore, we assessed the effect of azo dye contamination (Reactive Black 5, 30 mg kg −1 dry soil), bacteria that decolorize this dye and dye + bacteria in the presence or absence of maize leaf litter on soil respiration, soil inorganic N and microbial biomass. We found that dye contamination did not induce any change in soil respiration, soil microbial biomass or soil inorganic N availability ( P  > 0.05). Litter evidently increased soil respiration. Our study concludes that the Reactive Black 5 azo dye (applied in low amount, i.e., 30 mg kg −1 dry soil) contamination did not modify organic matter decomposition, N mineralization and microbial biomass in a silty loam soil.

Soil salinity is the major abiotic stress that disrupts nutrient uptake, hinders plant growth, and threatens agricultural production. Plant growth-promoting rhizobacteria (PGPR) are the most promising eco-friendly beneficial microorganisms that can be used to improve plant responses against biotic and abiotic stresses. In this study, a previously identified B. thuringiensis PM25 showed tolerance to salinity stress up to 3 M NaCl. The Halo-tolerant Bacillus thuringiensis PM25 demonstrated distinct salinity tolerance and enhance plant growth-promoting activities under salinity stress. Antibiotic-resistant Iturin C ( ItuC ) and bio-surfactant-producing ( sfp and srfAA ) genes that confer biotic and abiotic stresses were also amplified in B. thuringiensis PM25. Under salinity stress, the physiological and molecular processes were followed by the over-expression of stress-related genes (APX and SOD) in B. thuringiensis PM25. The results detected that B. thuringiensis PM25 inoculation substantially improved phenotypic traits, chlorophyll content, radical scavenging capability, and relative water content under salinity stress. Under salinity stress, the inoculation of B. thuringiensis PM25 significantly increased antioxidant enzyme levels in inoculated maize as compared to uninoculated plants. In addition, B. thuringiensis PM25-inoculation dramatically increased soluble sugars, proteins, total phenols, and flavonoids in maize as compared to uninoculated plants. The inoculation of B. thuringiensis PM25 significantly reduced oxidative burst in inoculated maize under salinity stress, compared to uninoculated plants. Furthermore, B. thuringiensis PM25-inoculated plants had higher levels of compatible solutes than uninoculated controls. The current results demonstrated that B. thuringiensis PM25 plays an important role in reducing salinity stress by influencing antioxidant defense systems and abiotic stress-related genes. These findings also suggest that multi-stress tolerant B. thuringiensis PM25 could enhance plant growth by mitigating salt stress, which might be used as an innovative tool for enhancing plant yield and productivity.

Emergence of multidrug resistant pathogens is increasing globally at an alarming rate with a need to discover novel and effective methods to cope infections due to these pathogens. Green nanoparticles have gained attention to be used as efficient therapeutic agents because of their safety and reliability. In the present study, we prepared zinc oxide nanoparticles (ZnO NPs) from aqueous leaf extract of Acacia arabica . The nanoparticles produced were characterized through UV-Visible spectroscopy, scanning electron microscopy, and X-ray diffraction. In vitro antibacterial susceptibility testing against foodborne pathogens was done by agar well diffusion, growth kinetics and broth microdilution assays. Effect of ZnO NPs on biofilm formation (both qualitatively and quantitatively) and exopolysaccharide (EPS) production was also determined. Antioxidant potential of green synthesized nanoparticles was detected by DPPH radical scavenging assay. The cytotoxicity studies of nanoparticles were also performed against HeLa cell lines. The results revealed that diameter of zones of inhibition against foodborne pathogens was found to be 16–30 nm, whereas the values of MIC and MBC ranged between 31.25–62.5 μg/ml. Growth kinetics revealed nanoparticles bactericidal potential after 3 hours incubation at 2 × MIC for E . coli while for S . aureus and S . enterica reached after 2 hours of incubation at 2 × MIC, 4 × MIC, and 8 × MIC. 32.5–71.0% inhibition was observed for biofilm formation. Almost 50.6–65.1% (wet weight) and 44.6–57.8% (dry weight) of EPS production was decreased after treatment with sub-inhibitory concentrations of nanoparticles. Radical scavenging potential of nanoparticles increased in a dose dependent manner and value ranged from 19.25 to 73.15%. Whereas cytotoxicity studies revealed non-toxic nature of nanoparticles at the concentrations tested. The present study suggests that green synthesized ZnO NPs can substitute chemical drugs against antibiotic resistant foodborne pathogens.

The rapid increase in infrastructural development in populated areas has had numerous adverse impacts. The rise in land surface temperature (LST) and its associated damage to urban ecological systems result from urban development. Understanding the current and future LST phenomenon and its relationship to landscape composition and land use/cover (LUC) changes is critical to developing policies to mitigate the disastrous impacts of urban heat islands (UHIs) on urban ecosystems. Using remote sensing and GIS data, this study assessed the multi-scale relationship of LUCC and LST of the cosmopolitan exponentially growing area of Beijing, China. We investigated the impacts of LUC on LST in urban agglomeration for a time series (2004–2019) of Landsat data using Classification and Regression Trees (CART) and a single channel algorithm (SCA), respectively. We built a CA–Markov model to forecast future (2025 and 2050) LUCC and LST spatial patterns. Our results indicate that the cumulative changes in an urban area (UA) increased by about 908.15 km2 (5%), and 11% of vegetation area (VA) decreased from 2004 to 2019. The correlation coefficient of LUCC including vegetation, water bodies, and built-up areas with LST had values of r = −0.155 (p > 0.419), −0.809 (p = 0.000), and 0.526 (p = 0.003), respectively. The results surrounding future forecasts revealed an estimated 2309.55 km2 (14%) decrease in vegetation (urban and forest), while an expansion of 1194.78 km2 (8%) was predicted for a built-up area from 2019 to 2050. This decrease in vegetation cover and expansion of settlements would likely cause a rise of about ~5.74 °C to ~9.66 °C in temperature. These findings strongly support the hypothesis that LST is directly related to the vegetation index. In conclusion, the estimated overall increase of 7.5 °C in LST was predicted from 2019–2050, which is alarming for the urban community’s environmental health. The present results provide insight into sustainable environmental development through effective urban planning of Beijing and other urban hotspots.

Mercury and cadmium are highly dangerous metals that can lead to disastrous effects in animals and humans. The aim of the current research was to elucidate the poisonous effects of mercuric chloride and cadmium chloride individually and in combination on biochemical profiles of plasma and their accumulation in heart. The therapeutic effect of vitamin C against these metals in rabbits was also studied. Mercuric chloride (1.2 μg/g), cadmium chloride (1.5 μg/g), and vitamin C (150 μg/g of body weight) were orally given to treatment groups of the rabbits (1—control; 2—vitamin; 3—CdCl 2 ; 4—HgCl 2 ; 5—vitamin + CdCl 2 ; 6—vitamin + HgCl 2 ; 7—CdCl 2 + HgCl 2 , and 8—vitamin + CdCl 2 + HgCl 2 . After the biometric determination of all intoxicated rabbits, biochemical parameters, viz low-density lipoproteins (LDL), high-density lipoproteins (HDL), cholesterol, creatine kinase, and troponin T (TnT) were analyzed using available kits. Levels of cholesterol (0.7 ± 0.1 mmol/l), creatine kinase (2985.2 ± 11 IU/L), LDL (20.35 ± 1.31 mg/dl), and troponin T (1.22 ± 0.03 μg/l) were significantly ( P  < 0.05) increased. HDL (84.78 ± 4.30 mg/dl) was significantly ( P  < 0.05) decreased, while supplementation of vitamin C decreased the adverse effects of CdCl 2 and HgCl 2 on biochemical parameters in all metal-exposed groups. A similar trend was also seen in rabbits treated with CdCl 2 + vitamin and vitamin + CdCl 2 + HgCl 2 . Accumulation of Cd and Hg was higher in heart tissues. This study, therefore, provides awareness on the cardiac toxicity of mercury and cadmium chlorides in the rabbits and the possible protective role of vitamin C against the perturbations induced by metals.

Rapid urbanization poses a threat to various ecosystem services. Beijing has undergone extensive infrastructure development in recent years. The study aims to extract land surface temperature (LST) and land use cover (LUC) data from satellite imagery, identify urban heat island (UHI) areas in Beijing, and determine the correlation between LST, LUC, NDVI, and BUI. It will also investigate the relationship between UHI and built/unbuilt areas, evaluate thermal comfort in Beijing using UTFVI, and assess the ecological quality of different land use types using the Ecological Evaluation Index (EEI). The results can inform urban planning and management in rapidly urbanizing and climate-changing regions. Changes in LUC and other activities affect the distribution of LST. For the study years (2005–2020), the estimated mean LST in Beijing was 24.72 °C, 27.07 °C, 26.22 °C, and 27.03 °C, respectively. A significant positive correlation (r = 0.96 p > 0.005) was found between LST and urban areas with other infrastructures. Geographically weighted regression (GWR) outperformed with Adj R2 > 0.74, suggesting that the extent of an urban heat island (UHI) is strongly dependent on the settlements, LUC composition, size, and terrain of surrounding communities. Urban hotspots in the city were identified and validated using Google Earth imagery. The Ecological Evaluation Index (EEI) value was relatively low compared to other ecosystem-related units. EEI showed a continuous increase of six percent in the most negative categories, indicating an unstable environment. This study concludes that urbanization affects the city’s environment, and study findings would help to regulate the urban ecosystem in Beijing.

Agriculture is directly linked to human life, providing food for survival and health. It is threatened by a number of challenges, such as climate change, resource depletion, and abiotic stresses, including heavy metals (HMs), salinity, drought, etc. Various strategies have been employed to palliate the phytotoxic effects of these stressors from the soil–plant system. Nanotechnological approaches have emerged as a promising tool for increasing crop productivity and promoting sustainable agriculture. Interestingly, the seed nano-priming approach has shown potential against all of the above-mentioned abiotic stress factors and has improved crop productivity. The application of nanoparticles (NPs) via seed priming is an innovative and cost-effective approach that improves seed germination and subsequent plant growth by activating plant physiological processes and providing tolerance against various stresses. The seed priming with NPs induces electron exchange and increases surface reaction capabilities related to plant cell and tissue components. This review aims to provide an overview of recent advances and research findings on seed nano-priming and the possible mechanism of plant stress-tolerance augmentation against various stresses. Furthermore, we also shed light on gaps in studies conducted in previous years, which will open new avenues for future research.

Plant microbiome (or phytomicrobiome) engineering (PME) is an anticipated untapped alternative strategy that could be exploited for plant growth, health and productivity under different environmental conditions. It has been proven that the phytomicrobiome has crucial contributions to plant health, pathogen control and tolerance under drastic environmental (a)biotic constraints. Consistent with plant health and safety, in this article we address the fundamental role of plant microbiome and its insights in plant health and productivity. We also explore the potential of plant microbiome under environmental restrictions and the proposition of improving microbial functions that can be supportive for better plant growth and production. Understanding the crucial role of plant associated microbial communities, we propose how the associated microbial actions could be enhanced to improve plant growth-promoting mechanisms, with a particular emphasis on plant beneficial fungi. Additionally, we suggest the possible plant strategies to adapt to a harsh environment by manipulating plant microbiomes. However, our current understanding of the microbiome is still in its infancy, and the major perturbations, such as anthropocentric actions, are not fully understood. Therefore, this work highlights the importance of manipulating the beneficial plant microbiome to create more sustainable agriculture, particularly under different environmental stressors.

This study aims to explore the support and impede factors of entrepreneurial activities amidst the COVID-19 pandemic. By using the qualitative method, 18 in-depth interviews were conducted with entrepreneurs operating micro-, small- and medium-scale enterprises. Interviews were transcribed for content analysis to generate themes using NVivo 12. COVID-19 has adversely affected the entrepreneurial activity in Pakistan was a key theme found after analysis. Three main categories were found as situations provoking business decline and their manifestation, entrepreneurial actions and reactions to COVID-19 crisis, and their futuristic plans amidst COVID-19. This research highlights issues entrepreneurs face to follow protocols of lockdown, social distancing, and operational hours. The findings of this study contribute to the scholarship of entrepreneurship and areas for the empirical investigation to develop efficient ecosystems to support entrepreneurs. This study suggests government and non-government stakeholders devise strategies for entrepreneurial revival post-COVID-19. This is probably one of the first qualitative assessment of the likely effects of the COVID-19 pandemic on entrepreneurship. It also recommends interesting related research areas and suggestions on how to empower entrepreneurs to overcome it.

Stenotrophomonas maltophilia is a multidrug resistant pathogen associated with high mortality and morbidity in patients having compromised immunity. The efflux systems of S . maltophilia include SmeABC and SmeDEF proteins, which assist in acquisition of multiple-drug-resistance. In this study, proteome based mapping was utilized to find out the potential drug targets for S . maltophilia strain k279a. Various tools of computational biology were applied to remove the human-specific homologous and pathogen-specific paralogous sequences from the bacterial proteome. The CD-HIT analysis selected 4315 proteins from total proteome count of 4365 proteins. Geptop identified 407 essential proteins, while the BlastP revealed approximately 85 non-homologous proteins in the human genome. Moreover, metabolic pathway and subcellular location analysis were performed for essential bacterial genes, to describe their role in various cellular processes. Only two essential proteins (Acyl-[acyl-carrier-protein]—UDP-N acetyl glucosamine O-acyltransferase and D-alanine-D-alanine ligase) as candidate for potent targets were found in proteome of the pathogen, in order to design new drugs. An online tool, Swiss model was employed to model the 3D structures of both target proteins. A library of 5000 phytochemicals was docked against those proteins through the molecular operating environment (MOE). That resulted in to eight inhibitors for both proteins i.e. enterodiol, aloin, ononin and rhinacanthinF for the Acyl-[acyl-carrier-protein]—UDP-N acetyl glucosamine O-acyltransferase, and rhazin, alkannin beta, aloesin and ancistrocladine for the D-alanine-D-alanine ligase. Finally the ADMET was done through ADMETsar. This study supported the development of natural as well as cost-effective drugs against S . maltophilia . These inhibitors displayed the effective binding interactions and safe drug profiles. However, further in vivo and in vitro validation experiment might be performed to check their drug effectiveness, biocompatibility and their role as effective inhibitors.