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Red hybrid tilapia were fed a formalin-killed oral Streptococcus iniae vaccine (FKV) in the present study was assessed. Three hundred Red hybrid tilapia 80 ± 10 g were divided into five groups (1A, 1B, 2A, 2B, and Cx), each consisting of 60 fish. Fish from Groups 1A, 1B, 2A, and 2B were fed with FKV over different periods of administration, while Group 2B was the only group of fish to receive an oral booster vaccination on day 14- and 21-days post-vaccination (dpv). Group Cx was fed with normal pellets containing no vaccine as a control group. At four weeks post-vaccination (wpv), all fish were experimentally infected with S. iniae. Groups 2A and 2B had the lowest level of mortalities following vaccination (45% and 30%, respectively) compared to Groups 1A and 1B (80% and 55%, respectively), while the level of mortalities in Group Cx was 100%. All vaccinated groups showed a significant increase in anti-S. iniae IgM levels (p < 0.05) in serum, mucus, and gut-lavage, while Group Cx did not (p > 0.05) and all fish in this group died by five weeks post-infection. In conclusion, fish fed with the S. iniae FKV had a greater level of protection against S. iniae, with increased specific antibody response to the vaccine and there was also evidence of GALT stimulation by the vaccine.

This study investigates the clinicopathological features of acute experimental streptococcosis in red tilapia using various routes of infection; intraperitoneal (IP), immersion (IM) and immersion cut (IC). Twenty four red tilapia in duplicates were inoculated intraperitoneally with 10 9 CFU/ml of S. agalactiae while another sets: intact, one with sharp cut at the tail end were exposed to bacterial inoculums 10 9 CFU/ml diluted in water while two groups of control fish were similarly manipulated. Clinical signs were recorded; samples from the gills, brain, eyes and kidneys were also taken for bacterial isolation and histopathology. Immunohistochemistry (IHC) and polymerase chain reaction (PCR) were employed to detect the antigen. The diseased fish showed skin, fin haemorrhages and exophthalmia with obvious signs in IP at 2 hpc followed by IC and IM at 4 hpc. The lesions were noticed earlier in the kidney and most severe in IP. IHC detected antigen as early as PCR and isolation with intense staining in blood vessel lumen and wall, macrophages in choroid, focal haemorrhage in the renal interstitium and meninges especially in IP followed by IC and IM. The immunolocalisation of the antigen described for the first time further explain the pathogenesis of streptococcosis in red tilapia.

Network function virtualization (NFV) is a rapidly growing technology that enables the virtualization of traditional network hardware components, offering benefits such as cost reduction, increased flexibility, and efficient resource utilization. Moreover, NFV plays a crucial role in sensor and IoT networks by ensuring optimal resource usage and effective network management. However, adopting NFV in these networks also brings security challenges that must promptly and effectively address. This survey paper focuses on exploring the security challenges associated with NFV. It proposes the utilization of anomaly detection techniques as a means to mitigate the potential risks of cyber attacks. The research evaluates the strengths and weaknesses of various machine learning-based algorithms for detecting network-based anomalies in NFV networks. By providing insights into the most efficient algorithm for timely and effective anomaly detection in NFV networks, this study aims to assist network administrators and security professionals in enhancing the security of NFV deployments, thus safeguarding the integrity and performance of sensors and IoT systems.

The Fourth Industrial Revolution (Industry 4.0) has profoundly transformed industries worldwide through the integration of advanced digital technologies, including artificial intelligence, digital twins, building information modeling (BIM), and the Internet of Things (IoT). The Architecture, Construction, and Engineering (ACE) sectors are increasingly adopting these innovations to meet the evolving demands of the global market. Within this dynamic context, Saudi Arabia has emerged as a front-runner and significant investor in this sector, as evidenced by the launch of ambitious mega-projects such as NEOM and The Line. These developments prompt valuable discussions about the readiness of graduates to adapt to rapid technological advancements and meet the current demands of the Saudi market. Although numerous studies have explored this issue, the Saudi context presents unique challenges and opportunities due to the accelerated pace of change within the ACE sectors, driven by the goals of Vision 2030. For this reason, this paper aims to address this gap by exploring the readiness of architectural programs in the context of Saudi Arabia to meet the demands of Industry 4.0. To achieve this, a comprehensive literature review was conducted, developing an analytical framework. Subsequently, a multiple-cases approach was employed, with an overall top-level discussion on the undergraduate architecture program subjects available in the five regions in Saudi Arabia. A combination of field observations, domain expertise, and evidence-based coding methods was employed to develop the SWOT analysis. The SWOT framework was utilized to identify key strengths, weaknesses, opportunities, and threats within the current academic programs. The findings were then analyzed in a comprehensive discussion, highlighting necessary transformations in existing programs. The methodology employed in our study involves prolonged engagement and persistent observation to enhance the quality and credibility of the discussion. This paper serves as a roadmap for guiding future educational reforms and aligning architectural education with emerging industry demands and technological advancements in the field. Four key themes are essential for aligning architectural education with Industry 4.0: sustainability in the built environment, innovation and creativity, digital applications in the built environment, and entrepreneurship and leadership in venture engineering. It also strongly emphasized sustainability courses and noted notable deficiencies in preparing students for a digitally driven professional landscape. For example, the average program comprises 162 credit hours and 58 courses, with only six related to Industry 4.0. The top five institutions offering Industry 4.0 courses ranked from highest to lowest are ARCH-U11, ARCH-U8, ARCH-U3, ARCH-U4, and ARCH-U15. ARCH-U11 offers the most Industry 4.0 courses, totaling 15, which account for 26.8% of its courses and 15% of its credit hours, in contrast to ARCH-U20, which offers no courses. The novelty of this research lies in its comprehensive analysis of the readiness of architecture program curricula from 20 Saudi universities to meet the requirements of Industry 4.0. Importantly, these findings support previous studies that established guidelines that mandate the inclusion of sustainability, innovation, and digital skills in architectural education programs. Contribution to the knowledge and findings is valuable for educational institutions, policymakers, and industry leaders, offering insights into evolving architectural education to meet future industry demands and foster technological innovation and sustainable development. Moreover, it provides actionable recommendations for curriculum development in alignment with Vision 2030. Contrary to expectations, findings show that lower-ranked universities offer more Industry 4.0-related courses than higher-ranked ones, emphasizing the need to align university evaluation standards with labor market demands.

AmBisome therapy for VL has an excellent efficacy and safety profile and has been adopted as a first-line regimen in Bangladesh. Second-line treatment options are limited and should preferably be given in short course combinations in order to prevent the development of resistant strains. Combination regimens including AmBisome, paromomycin and miltefosine have proved to be safe and effective in the treatment of VL in India. In the present study, the safety and efficacy of these same combinations were assessed in field conditions in Bangladesh. The safety and efficacy of three combination regimens: a 5 mg/kg single dose of AmBisome + 7 subsequent days of miltefosine (2.5 mg/kg/day), a 5 mg/kg single dose of AmBisome + 10 subsequent days of paromomycin (15 mg/kg/day) and 10 days of paromomycin (15 mg/kg/day) + miltefosine (2.5 mg/kg/day), were compared with a standard regimen of AmBisome 15 mg/kg given in 5 mg/kg doses on days 1, 3 and 5. This was a phase III open label, individually randomized clinical trial. Patients from 5 to 60 years with uncomplicated primary VL were recruited from the Community Based Medical College Bangladesh (CBMC,B) and the Upazila Health Complexes of Trishal, Bhaluka and Fulbaria (all located in Mymensingh district), and randomly assigned to one of the treatments. The objective was to assess safety and definitive cure at 6 months after treatment. 601 patients recruited between July 2010 and September 2013 received either AmBisome monotherapy (n = 158), AmBisome + paromomycin (n = 159), AmBisome + miltefosine (n = 142) or paromomycin + miltefosine (n = 142). At 6 months post- treatment, final cure rates for the intention-to-treat population were 98.1% (95%CI 96.0-100) for AmBisome monotherapy, 99.4% (95%CI 98.2-100) for the AmBisome + paromomycin arm, 94.4% (95%CI 90.6-98.2) for the AmBisome + miltefosine arm, and 97.9% (95%CI 95.5-100) for paromomycin + miltefosine arm. There were 12 serious adverse events in the study in 11 patients that included 3 non-study drug related deaths. There were no relapses or PKDL up to 6 months follow-up. All treatments were well tolerated with no unexpected side effects. Adverse events were most frequent during treatment with miltefosine + paromomycin, three serious adverse events related to the treatment occurred in this arm, all of which resolved. None of the combinations were inferior to AmBisome in both the intention-to-treat and per-protocol populations. All the combinations demonstrated excellent overall efficacy, were well tolerated and safe, and could be deployed under field conditions in Bangladesh. The trial was conducted by the International Centre for Diarrhoeal Disease Research (ICDDR,B) and the Shaheed Suhrawardy Medical College (ShSMC), Dhaka, in collaboration with the trial sites and sponsored by the Drugs for Neglected Diseases initiative (DNDi). ClinicalTrials.gov NCT01122771.

This study aims to investigate new tissue conditioner (TC) formulations involving chitosan nanoparticles (CSNPs) and essential oils (EO) for their antifungal potential, release kinetics, and hardness. CSNPs were synthesized, and the separate solutions of CSNP were prepared with two types of EO, i.e., Oregano oil and Lemongrass. The EO was loaded separately in two concentrations (200 [mu]L and 250 [mu]L). The blank and EO-loaded CSNPs were screened against Candida albicans (C. albicans), and their minimum inhibitory concentration was established. GC Reline.sup.[TM] (GC corporation, USA) TC was considered a control group, whereby the four experimental groups were prepared by mixing CSNPs/EO solutions with TC powder. The antifungal effectiveness (C. albicans) and release kinetics behavior (1-6 h, 24 h, 48 h, and 72 h) was investigated. The Shore A hardness of control and experimental groups was evaluated in dry and wet modes (deionized water and artificial saliva). For statistical analysis, SPSS version 22 was used to do a one-way ANOVA post-hoc Tukey's test. Compared to the control group, TCs containing blank CSNPs and CSNPs loaded with EO showed 3 and 5 log reductions in C. albicans growth, respectively. A significantly high antifungal effect was observed with TC containing lemongrass essential oil (200 [mu]L). The continuous release of EO was detected for the first 6 hours, whereas completely stopped after 48 hours. Mean hardness values were highest for dry samples and lowest for samples stored in artificial saliva. The statistically significant difference within and between the study groups was observed in mean and cumulative essential oils release and hardness values of TCs over observed time intervals irrespective of storage media. TCs containing essential-oil-loaded CSNPs seem a promising alternative treatment of denture-induced stomatitis, however, a further biological analysis should be taken.

Laser forming is an innovative technique that uses a defocused laser beam to form sheet metal by thermal stresses rather than external forces. This offers excellent and promising potential applications in rapid prototyping, straightening, aligning, and adjusting of macro/micrometallic components. However, the undesirable edge effects in laser forming reflect that the bending angle is not constant along the scanning line. This paper presents an analytical study of edge effects in laser bending of AISI 304 stainless steel plate. Experimental and numerical investigations aimed at understanding the effects of the triangular beam geometry with different aspect ratios were clearly demonstrated. A validated thermal model was developed, and different sets of FE simulations were carried out by varying heat input values and aspect ratio of laser beam with constant scanning speed. It is evident that triangular beam with highest aspect ratio was preferable to produce a higher bending angle with lesser edge effect at higher power intensity. It is found that triangular beam geometries are more effective in minimizing the bending angle variation compared with the circular beam.

The internet of things (IoT) is a complex system that includes multiple technologies and services. However, its heterogeneity can result in quality-of-service (QoS) issues, which may lead to security challenges. Software-defined network (SDN) provides unique solutions to handle heterogeneity issues in large-scale IoT networks. Combining SDN with IoT networks has great potential for addressing extreme heterogeneity issues in IoT networks. Numerous researchers are investigating various techniques to resolve heterogeneity issues in IoT networks by integrating SDN. Our study focuses on the SDN-IoT domain to improve QoS by addressing heterogeneity. Heterogeneity in SDN-IoT networks can increase the response time of controllers. We propose a framework that can alleviate heterogeneity while maintaining QoS in SDN-IoT networks. The framework converts m heterogeneous controllers into n homogeneous groups based on their response time. First, we examine the impact of the controller’s bandwidth and find that the system throughput decreases when the controller’s bandwidth is lowered. Next, we implement a simple strategy that considers both the bandwidth and service time when selecting the peer controller. Our results show some improvement in the framework, indicating its potential to alleviate heterogeneity while maintaining QoS and other metrics.

This retrospective cohort study aims to describe the clinical characteristics and outcomes and assess risk factors for mortality across the epidemic waves in hospitalized COVID-19 patients in a major tertiary-care center in Pakistan. A total of 5368 patients with COVID-19, hospitalized between March 2020 and April 2022 were included. The median age was 58 years (IQR: 44–69), 41% were females, and the overall mortality was 12%. Comparative analysis of COVID-19 waves showed that the proportion of patients aged ≥ 60 years was highest during the post-wave 4 period (61.4%) and Wave 4 (Delta) (50%) ( p  < 0.001). Male predominance decreased from 65.2% in Wave 2 to 44.2% in Wave 5 (Omicron) ( p  < 0.001). Mortality rate was lowest at 9.4% in wave 5 and highest at 21.6% in the post-wave 4 period ( p  = 0.041). In multivariable analysis for risk factors of mortality, acute respiratory distress syndrome (ARDS) was most strongly associated with mortality (aOR 22.98, 95% CI 15.28–34.55, p  < 0.001), followed by need for mechanical ventilation (aOR 6.81, 95% CI 5.13–9.05, p  < 0.001). Other significant risk factors included acute kidney injury (aOR 3.05, 95% CI 2.38–3.91, p  < 0.001), stroke (aOR 2.40, 95% CI 1.26–4.60, p = 0.008), pulmonary embolism (OR 2.07, 95% CI 1.28–3.35, p  = 0.003), and age ≥ 60 years (aOR 2.45, 95% CI 1.95–3.09, p  < 0.001). Enoxaparin use was associated with lower mortality odds (aOR 0.45, 95% CI 0.35–0.60, p  < 0.001. Patients hospitalized during Wave 4 (aOR 2.22, 95% CI 1.39–3.56, p  < 0.001) and the post-wave 4 period (aOR 2.82, 95% CI 1.37–5.80, p  = 0.005) had higher mortality odds compared to other waves. The study identifies higher mortality risk in patients admitted in Delta wave and post-wave, aged ≥ 60 years, and with respiratory and renal complications, and lower risk with anticoagulation during COVID-19 waves.

Background The promotional strategies implemented by pharmaceutical organizations lead to abnormal prescribing practices that both diminish physician-patient confidence and drive-up healthcare expenses. The motives of the pharmaceutical companies are to appeal students’ natural preferences. So, educating them represents an essential approach to combat these effects. Research provides few examples of educational programs while their long term sustained benefit has not been established over time. This research investigated how educational programs affect students in the short- and long-term after exposure to pharmaceutical promotional activities. Methods The study was conducted longitudinally across 7 schools within three provinces of Pakistan. Survey data collection took place from 856 medical students of the 3rd year class of term 2021–2022 and 2022–2023 for pre-post educational assessment. We performed two-year follow-up surveys which asked the same questions to these 2 cohorts to determine both the clinical rotations’ impact and various promotional methods on their responses. We measured the opinions of 819 students who completed their last year and did not take part in the educational intervention to compare with those taking it. Analysis of each individual point in all comparison sets occurred through SPSS version 23.0. Results Student perception along with business approach towards promotional strategies underwent significant modifications throughout the short-term period. The educational method lost its effectiveness at convincing students about avoiding financial support from pharmaceutical companies in the long-term period (median 2(1) to 2(0) p:0.049). Educated students displayed superior knowledge about how trivial gifts affect medication decisions over uneducated final year students (2 (0) to 3 (0) p  < 0.001). In the group with high promotion exposure the educated students-maintained awareness about trivial gift influences ( p  < 0.001) but uneducated students believed they were unaffected by such promotions ( p  < 0.001). Conclusion An education program could serve as a platform to build pharmaceutical promotion awareness alongside promoting skepticism levels and developing positive perceptions about pharmaceutical promotion methods. The educational intervention faces a high risk of becoming ineffective when students interact with the informal and hidden curriculum and exposure to promotional activities. The impact of role models, organizational culture, and institutional policies could be important aspects to be addressed for sustaining the effectiveness of such education programs.