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Improving the initial conditions of short-range numerical weather prediction (NWP) models is one of the main goals of the meteorological community. Development of data assimilation and ensemble forecast systems is essential in any national weather service (NWS). In this sense, the local ensemble transform Kalman filter (LETKF) is a methodology that can satisfy both requirements in an efficient manner. The Weather Research and Forecasting (WRF) Model coupled with the LETKF, developed at the University of Maryland, College Park, have been implemented experimentally at the NWS of Argentina [Servicio Meteorológico Nacional (SMN)], but at a somewhat lower resolution (40 km) than the operational Global Forecast System (GFS) at that time (27 km). The purpose of this work is not to show that the system presented herein is better than the higher-resolution GFS, but that its performance is reasonably comparable, and to provide the basis for a continued improved development of an independent regional data assimilation and forecasting system. The WRF-LETKF system is tested during the spring of 2012, using the prepared or quality controlled data in Binary Universal Form for Representation of Meteorological Data (PREPBUFR) observations from the National Centers for Environmental Prediction (NCEP) and lateral boundary conditions from the GFS. To assess the effect of model error, a single-model LETKF system (LETKF-single) is compared with a multischeme implementation (LETKF-multi), which uses different boundary layer and cumulus convection schemes for the generation of the ensemble of forecasts. The performance of both experiments during the test period shows that the LETKF-multi usually outperforms the LETKF-single, evidencing the advantages of the use of the multischeme approach. Both data assimilation systems are slightly worse than the GFS in terms of the synoptic environment representation, as could be expected given their lower resolution. Results from a case study of a strong convective system suggest that the LETKF-multi improves the location of the most intense area of precipitation with respect to the LETKF-single, although both systems show an underestimation of the total accumulated precipitation. These preliminary results encourage continuing the development of an operational data assimilation system based on WRF-LETKF at the SMN.

On December 8th, 2022, a meteotsunami caused significant damage to the southern beaches of Mar del Plata city, Argentina, and the Montevideo Port in Uruguay. The event was observed over a wide area, from Puerto Belgrano in the south of Buenos Aires Province, Argentina, to La Paloma, Uruguay, including locations in the upper Río de la Plata estuary. The meteotsunami was driven by a relatively long perturbation of the surface atmospheric pressure caused by the movement of an elongated high-pressure anomaly running parallel to a low-pressure system associated with an atmospheric frontal zone. The atmospheric perturbation propagated faster than the meteotsunami between Mar del Plata and Montevideo, but both velocities may have been similar between Mar del Plata and Santa Teresita, where the maximum meteotsunami wave height of 0.92 m was recorded, likely due to the Proudman resonance mechanism. The propagation direction of the front from SW to NE partially explains the negligible response of the water level on the Argentinean Río de la Plata coast, where observation sites are close to the shore from where the forcing originates, as well as the almost identical starting time of the water level activity in the upper Río de la Plata and the outer sector of the estuary. This event was different from other past events in the study region, as there was no evidence of atmospheric gravity wave propagation. One significant limitation in understanding the observed atmosphere–ocean interaction in the study region is the nonexistence of high-frequency atmospheric pressure data.

Cesarean delivery rates continue to increase worldwide and reached 57% in Brazil in 2014. Although the safety of this surgery has improved in the last decades, this trend is a concern because it carries potential risks to women's health and may be a modifiable risk factor of maternal mortality. This paper aims to investigate the risk of postpartum maternal death directly associated with cesarean delivery in comparison to vaginal delivery in Brazil. This was a population-based case-control study performed in eight Brazilian states. To control for indication bias, deaths due to antenatal morbidity were excluded. We included 73 cases of postpartum maternal deaths from 2009-2012. Controls were selected from the Birth in Brazil Study, a 2011 nationwide survey including 9,221 postpartum women. We examined the association of cesarean section and postpartum maternal death by multivariate logistic regression, adjusting for confounders. After controlling for indication bias and confounders, the risk of postpartum maternal death was almost three-fold higher with cesarean than vaginal delivery (OR 2.87, 95% CI 1.63-5.06), mainly due to deaths from postpartum hemorrhage and complications of anesthesia. Cesarean delivery is an independent risk factor of postpartum maternal death. Clinicians and patients should consider this fact in balancing the benefits and risks of the procedure.

Background/Objectives: Macrophages with the M2 phenotype are an immune population with great relevance for tumor development. We have previously demonstrated that mesenchymal stromal cells (MSCs) from cervical cancer (CeCa-MSCs) enhance the immunomodulatory activity of CeCa cells on T lymphocytes; however, the effect of these cells on the ability of tumor cells to polarize macrophages had not been evaluated to date. Methods: To address this, we set out to analyze the effect of normal cervix (NCx) and CeCa-MSCs interacting with CeCa tumor cells (TCs) to polarize macrophages in a coculture system. Results: Our results show that macrophages from TC/NCx-MSC cocultures decreased CD163 expression. In turn, we observed that macrophages from TC/CeCa-MSC cocultures, in contrast to those in the presence of TCs/NCx-MSCs, increased the intracellular production of IDO, IL-4, and IL-10; decreased T lymphocyte proliferation; and increased the presence of soluble IL-10. Interestingly, coculture in the presence of TCs/NCx-MSCs decreased the capacity of macrophages to generate regulatory T lymphocyte populations, as well as their phagocytic capacity, and increased IL-6 secretion, unlike the coculture of macrophages in the presence of TCs/CeCa-MSCs. Our results show that TCs/CeCa-MSCs in cocultures, unlike TCs/NCx-MSCs, have a greater capacity to polarize macrophages to an M2 phenotype and that such macrophages have a greater immunosuppressive potential. Conclusions: This in vitro study suggests that intracellular communication between MSCs and tumor cells in CeCa may promote tumor growth through the polarization of macrophages with increased immunosuppressive activity.

Cellular homeostasis is lost or becomes dysfunctional during septic shock due to the activation of the inflammatory response and the deregulation of oxidative stress. Antioxidant therapy administered alongside standard treatment could restore this lost homeostasis. We included 131 patients with septic shock who were treated with standard treatment and vitamin C (Vit C), vitamin E (Vit E), N-acetylcysteine (NAC), or melatonin (MT), in a randomized trial. Organ damage quantified by Sequential Organ Failure Assessment (SOFA) score, and we determined levels of Interleukins (IL) IL1β, Tumor necrosis factor alpha (TNFα), IL-6, monocyte chemoattractant protein-1 (MCP-1), Transforming growth factor B (TGFβ), IL-4, IL-10, IL-12, and Interferon-γ (IFNγ). The SOFA score decreased in patients treated with Vit C, NAC, and MT. Patients treated with MT had statistically significantly reduced of IL-6, IL-8, MCP-1, and IL-10 levels. Lipid peroxidation, Nitrates and nitrites (NO3− and NO2−), glutathione reductase, and superoxide dismutase decreased after treatment with Vit C, Vit E, NAC, and MT. The levels of thiols recovered with the use of Vit E, and all patients treated with antioxidants maintained their selenium levels, in contrast with controls (p = 0.04). The findings regarding oxidative stress markers and cytokines after treatment with antioxidants allow us to consider to future the combined use of antioxidants in a randomized clinical trial with a larger sample to demonstrate the reproducibility of these beneficial effects.

Barnacles and their allies (Thecostraca) are a biologically diverse, monophyletic crustacean group, which includes both intensely studied taxa, such as the acorn and stalked barnacles, as well as cryptic taxa, for example, Facetotecta. Recent efforts have clarified phylogenetic relationships in many different parts of the barnacle tree, but the outcomes of these phylogenetic studies have not yet been combined into a single hypothesis for all barnacles. In the present study, we applied a new “synthesis” tree approach to estimate the first working Barnacle Tree of Life. Using this approach, we integrated phylogenetic hypotheses from 27 studies, which did not necessarily include the same taxa or used the same characters, with hierarchical taxonomic information for all recognized species. This first synthesis tree contains 2,070 barnacle species and subspecies, including 239 barnacle species with phylogenetic information and 198 undescribed or unidentified species. The tree had 442 bifurcating nodes, indicating that 79.3% of all nodes are still unresolved. We found that the acorn and stalked barnacles, the Thoracica, and the parasitic Rhizocephala have the largest amount of published phylogenetic information. About half of the thecostracan families for which phylogenetic information was available were polyphyletic. We queried publicly available geographic occurrence databases for the group, gaining a sense of geographic gaps and hotspots in our phylogenetic knowledge. Phylogenetic information is especially lacking for deep sea and Arctic taxa, but even coastal species are not fully incorporated into phylogenetic studies.

The Notch signaling pathway is a reiteratively used cell to cell communication pathway that triggers pleiotropic effects. The correct regulation of the pathway permits the efficient regulation of genes involved in cell fate decision throughout development. This activity relies notably on the CSL proteins, (an acronym for C BF-1/RBPJ-κ in Homo sapiens / Mus musculus respectively, S uppressor of Hairless in Drosophila melanogaster , L ag-1 in Caenorhabditis elegans ) which is the unique transcription factor and DNA binding protein involved in this pathway. The CSL proteins have the capacity to recruit activation or repression complexes according to the cellular context. The aim of this review is to describe the different co-repressor proteins that interact directly with CSL proteins to form repression complexes thereby regulating the Notch signaling pathway in animal cells to give insights into the paralogous evolution of these co-repressors in higher eumetazoans and their subsequent effects at developmental processes.

This study presents results of the preparation, absorption and emission characterizations of chlorophyll samples extracted from green microalgae, based on the importance of this pigment and its optical properties. In this work, we characterized extracts containing chlorophyll from green microalgae of the genus Chlorella (C. vulgaris and C. protothecoides) via absorption and emission spectroscopies. The present results were compared with those of chlorophyll extracted from grass, previously reported. Microalgal chlorophyll samples were covalently bonded to the pore surface of organo-modified silica xerogels for a major spectroscopic analysis. Our findings indicate that the composition of green microalgal extracts corresponds mainly to chlorophyll a with a minimal amount of β-carotene. UV-VIS, IR absorption and UV-VIS emission spectra of the samples displayed the typical bands of chlorophyll a, and some other bands ascribed to β-carotene. Both the obtained absorption and emission spectra identify the algae chlorophyll bands and are unique. An effect of a radiative energy transfer mechanism between the silica matrix and the microalgal chlorophyll was observed as reabsorption bands in the emission spectra, and this effect was more evident in the C. vulgaris sample with respect to C. protothecoides.

Inorganic arsenic (i-As) is a naturally occurring toxic metalloid affecting millions of people worldwide. It is known to be carcinogen, liver being a potential target, and related to the prevalence of diabetes in arseniasis-endemic areas. Hepatocyte nuclear factor 1 and 4 alpha (HNF1α and HNF4α) are key members of a transcriptional network essential for normal liver architecture. Changes in HNF1α and HNF4α expression are clearly associated with the development of liver malignancies and diabetes in humans. In this work, hepatic HepG2 cells and golden Syrian hamsters were exposed to sub-toxic, environmentally relevant doses of sodium arsenite (SA; up to 10 μM in vitro, 15 mg/L in vivo) in order to evaluate whether arsenic is able to compromise the expression of hepatocyte nuclear factors. Also, liver histopathological examination was carried out, and several markers of hepatocyte differentiation and glucose metabolism status were determined as a measure of i-As-induced effects. Results show a consistent down-regulation of HNF1α and HNF4α under a scenario of exposure where HepG2 cells (1) gained resistance to arsenic-induced toxicity/apoptosis, (2) attained loss of tissue-specific features (as shown by the observed down-regulation of ALDOB, PEPCK and CYP1A2, triggering of the epithelial-to-mesenchymal transition program and the hypersecretion of matrix metalloproteinase-2 and 9), (3) failed to maintain balanced expression of the “stemness” genes C-MYC, OCT3/4, LIN28 and NOTCH2 and (4) showed glucose metabolism impairment. We conclude that the i-As-induced down-regulation of HNF1α and HNF4α under chronic settings may play a central role in the features of disease and cancer observed both in vivo and in vitro.

The work tries on the fundamental characteristics of the observation like technique for acquisition of scientific information and realization of diagnostic in the family, and how the same one you triangula with the therapeutic interview. The objective is to offer a group of theoretical indications for the application of these techniques in the intervention in families. They take as relating investigative the conceptions of Aiken, Sommer, Andolfi and Cormier, among others. In the internal logic of the work, he/she is defined the observation in their connection the family orientation and which they are the abilities that the investigator should deploy, as well as the investigation types and observation registrations. In the case of the interview it is remarked the types of verbal and not verbal communication to settle down with the interviewee to make feasible the intervention and orientation of the family.