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To prevent accelerated thermal aging or insulation faults in cable systems due to overheating, the current carrying capacity is usually limited by specific conductor temperatures. As the heat produced during the operation of underground cables has to be dissipated to the environment, the actual current carrying capacity of a power cable system is primarily dependent on the thermal properties of the surrounding porous bedding material and soil. To investigate the heat dissipation processes around buried power cables of real scale and with realistic electric loading, a field experiment consisting of a main field with various cable configurations, laid in four different bedding materials, and a side field with additional cable trenches for thermally enhanced bedding materials and protection pipe systems was planned and constructed. The experimental results present the strong influences of the different bedding materials on the maximum cable ampacity. Alongside the importance of the basic thermal properties, the influence of the bedding’s hydraulic properties, especially on the drying and rewetting effects, were observed. Furthermore, an increase in ampacity between 25% and 35% was determined for a cable system in a duct filled with an artificial grouting material compared to a common air-filled ducted system.

A large share of the primary energy is consumed to provide space heating. Geothermal energy offers a regenerative alternative. For reasons of efficiency and environmental protection, it is important to ensure the system integrity of a borehole heat exchanger (BHE). Previous investigations have focused on the individual components of the BHE or on the grout and pipe systems’ integrity. This study focused on the analysis of the hydraulic system integrity of the complete subsoil–grout–pipe system as well as possible thermally induced changes. For this purpose, a pilot-scale experiment was built to test a 1-m section of a typical BHE under in situ pressure, hydraulic and temperature conditions. During the tests the hydraulic system permeability of the soil and the BHE was measured continuously and separately from each other. In addition, the temperature monitoring array was installed in a 50-cm cross-sectional area. Significant temperature-related fluctuations in the sealing performance could be observed. Hydraulic conductivity limits required by VDI 4640-2 (Thermal use of the underground—ground source heat pump systems, 2019) were exceeded without frost action. The succeeding application of freeze–thaw cycles further enhances the system permeability. The study shows that the thermally induced effects on the system integrity of the BHE are larger and more significant than the subsequent frost-induced effects. The hydrophobic character of the high-density polyethylene (PE-HD) pipes as well as its high coefficient of thermal expansion seem to be the main points of weakness in the system. Optimization research should focus on the interface connection between grout and pipe, whereby hydrophilic pipe materials such as stainless steel or aluminum should also be considered as well as manipulation of the pipe surface properties of PE-HD.

Background Using primary airway epithelial cells (AEC) is essential to mimic more closely different types and stages of lung disease in humans while reducing or even replacing animal experiments. Access to lung tissue remains limited because these samples are generally obtained from patients who undergo lung transplantation for end-stage lung disease or thoracic surgery for (mostly) lung cancer. We investigated whether forceps or cryo biopsies are a viable alternative source of AEC compared to the conventional technique. Methods AECs were obtained ex vivo from healthy donor lung tissue using the conventional method and two biopsy procedures (forceps, cryo). The influence of the isolation method on the quality and function of AEC was investigated at different time-points during expansion and differentiation in air-liquid interface cultures. In addition, fully-differentiated AECs were stimulated with house dust mite extract (HDM) to allow functional analyses in an allergic in vitro model. Vitality or differentiation capacity were determined using flow cytometry, scanning electron microscope, periodic acid-Schiff reaction, immunofluorescence staining, and proteomics. Results As anticipated, no significant differences between each of the sampling methods were detected for any of the measured outcomes. The proteome composition was comparable for each isolation method, while donor-dependent effects were observed. Treatment with HDM led to minor differences in mucociliary differentiation. Conclusions Our findings confirmed the adequacy of these alternative approaches for attaining primary AECs, which can now expand the research for a broader range of lung diseases and for studies at an earlier stage not requiring lung surgery.

The myotendinous junction (MTJ) is a critical interface between muscle fibers and tendons, essential for force transmission between muscle and bone. Laminin–α2, a key extracellular matrix (ECM) component, is strongly enriched at this interface. Mutations in the LAMA2 gene cause LAMA2–related muscular dystrophy (LAMA2 MD), an early-onset severe congenital muscular dystrophy. Here, we examined the MTJ in dyW/dyW mice, a mouse model for LAMA2 MD. We find a strong disruption of MTJ morphology, including altered muscle fiber tips, collagen XXII mislocalization, and reduced muscle tendon interface. As MTJ loading is altered in dyW/dyW mice and MTJ maintenance requires loading and unloading, we also examined MTJ structures upon denervation–induced unloading. While muscle fiber tip morphology resembled that of dyW/dyW mice, collagen XXII distribution was not affected and the muscle–tendon interface was preserved. Finally, proteomic profiling via laser capture microdissection and mass spectrometry revealed significant regional and global shifts in MTJ protein composition in dyW/dyW and denervated mice. Across both models, we identified integrin–associated remodeling as a shared response linked to the perturbed muscle fiber tip morphology. These findings demonstrate that laminin–α2 is required for MTJ stability, and that mechanical unloading contributes to the observed phenotype. Importantly, our results suggest that disruptions in MTJ structure and protein composition may contribute to the pathology observed in LAMA2 MD.Competing Interest StatementThe authors have declared no competing interest.Funder Information DeclaredSwiss National Science Foundation, 189248, 187415, 220245