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Bio-upcycling of plastics is an emerging alternative process that focuses on extracting value from a wide range of plastic waste streams. Such streams are typically too contaminated to be effectively processed using traditional recycling technologies. Medium-chain-length (mcl) diols and dicarboxylates (DCA) are major products of chemically or enzymatically depolymerized plastics, such as polyesters or polyethers. In this study, we enabled the efficient metabolism of mcl-diols and -DCA in engineered Pseudomonas putida as a prerequisite for subsequent bio-upcycling. We identified the transcriptional regulator GcdR as target for enabling metabolism of uneven mcl-DCA such as pimelate, and uncovered amino acid substitutions that lead to an increased coupling between the heterologous β-oxidation of mcl-DCA and the native degradation of short-chain-length DCA. Adaptive laboratory evolution and subsequent reverse engineering unravelled two distinct pathways for mcl-diol metabolism in P. putida , namely via the hydroxy acid and subsequent native β-oxidation or via full oxidation to the dicarboxylic acid that is further metabolized by heterologous β-oxidation. Furthermore, we demonstrated the production of polyhydroxyalkanoates from mcl-diols and -DCA by a single strain combining all required metabolic features. Overall, this study provides a powerful platform strain for the bio-upcycling of complex plastic hydrolysates to polyhydroxyalkanoates and leads the path for future yield optimizations. Graphical Abstract

Bio-upcycling of plastics is an emerging alternative process that focuses on extracting value from a wide range of plastic waste streams. Such streams are typically too contaminated to be effectively processed using traditional recycling technologies. Medium-chain-length (mcl) diols and dicarboxylates (DCA) are major products of chemically or enzymatically depolymerized plastics, such as polyesters or polyethers. In this study, we enabled the efficient metabolism of mcl-diols and -DCA in engineered Pseudomonas putida as a prerequisite for subsequent bio-upcycling. We identified the transcriptional regulator GcdR as target for enabling metabolism of uneven mcl-DCA such as pimelate, and uncovered amino acid substitutions that lead to an increased coupling between the heterologous [beta]-oxidation of mcl-DCA and the native degradation of short-chain-length DCA. Adaptive laboratory evolution and subsequent reverse engineering unravelled two distinct pathways for mcl-diol metabolism in P. putida, namely via the hydroxy acid and subsequent native [beta]-oxidation or via full oxidation to the dicarboxylic acid that is further metabolized by heterologous [beta]-oxidation. Furthermore, we demonstrated the production of polyhydroxyalkanoates from mcl-diols and -DCA by a single strain combining all required metabolic features. Overall, this study provides a powerful platform strain for the bio-upcycling of complex plastic hydrolysates to polyhydroxyalkanoates and leads the path for future yield optimizations.

A complete introduction tocar-to-X communications networking Automotive Inter-networking will introduce a range of new network and system technologies for vehicle safety, entertainment and comfort systems currently being researched and developed.

Recently, the relationship between Web 2.0 and service-oriented architectures (SOAs) has received an enormous amount of coverage because of the notion of complexity-hiding and reuse, along with the concept of loosely coupling services. Some argue that Web 2.0 and SOAs have significantly different elements and thus cannot be regarded as parallel philosophies. Others, however, consider the two concepts as complementary and regard Web 2.0 as the global SOA. This paper investigate these two philosophies and their respective applications from both a technological and business perspective

Cross-organizational electronic collaboration is about to gain significant momentum and facilitates the emergence of a globally networked information service economy. However, existing solutions for the realization of such business relationships still exhibit weaknesses with respect to both managerial and technological aspects. In this work, we propose a service-oriented reference architecture for electronic business media that overcomes the drawbacks of today’s business-to-business (B2B) software products and services. Based on the St. Gallen media reference model, this reference architecture incorporates the design principle of modularity that proved critical for the success of numerous artifacts in other more mature industries. In particular, we investigate and revisit the principle of modularity with respect to its role in the computer industry and transfer it to the context of organizing and implementing electronic cross-company collaboration. On the basis of a case study in the field of public administration in Switzerland, we show its real-world applicability and its improvement potential.

Cross-organizational electronic collaboration is about to gain significant momentum and facilitates the emergence of a globally networked information service economy. However, existing solutions for the realization of such business relationships still exhibit weaknesses with respect to both managerial and technological aspects. In this work, we propose a service-oriented reference architecture for electronic business media that overcomes the drawbacks of today's business-to-business (B2B) software products and services. Based on the St. Gallen media reference model, this reference architecture incorporates the design principle of modularity that proved critical for the success of numerous artifacts in other more mature industries. In particular, we investigate and revisit the principle of modularity with respect to its role in the computer industry and transfer it to the context of organizing and implementing electronic cross-company collaboration. On the basis of a case study in the field of public administration in Switzerland, we show its real-world applicability and its improvement potential.

A complete introduction tocar-to-X communications networking Automotive Inter-networking will introduce a range of new network and system technologies for vehicle safety, entertainment and comfort systems currently being researched and developed. C2X networking is not only a matter of technology, but is also very closely related to policy-making about deployment. This book will provide the background on technical developments but will also discuss the potential benefits, costs and risks. Also discussed will be concepts related to application of vehicle-to-vehicle and vehicle-to-infrastructure communication technologies for various purposes such as automobile safety enhancement, vehicle user applications for comfort and convenience and efficiency along with other potential commercial applications. Application domains will build the starting point for an analysis of the requirements on suitable mobile network technology and the book will look at how well existing and new systems match these requirements. New automotive-specific technologies are presented in detail, explaining millimeter wave short range systems and special automotive network protocols. Specially designed system services and security mechanisms are introduced and system architecture, radio spectrum use, medium access control, network protocols and security concepts and considered.  Finally, the book will present the current world-wide standardization activities, deployment strategies and an outlook about the evolvement of inter-vehicle communications in the next decades. Presents a comprehensive top-down approach to the newly evolving car-to-X communications networking Provides a broad overview of all relevant C2X communication topics Written by well known experts in the field Predicts the outlook of the evolvement of inter-vehicle communications in the next decades Includes illustrations and high-level technical sketches of application domains and photographs, 3D renderings and professional graphical sketches of current prototypes

The adoption of advanced integration technologies that enable private and public organizations to seamlessly execute their business transactions electronically is still relatively low, especially among governmental bodies and Small and Medium-sized Enterprises (SMEs). Current solutions often lack a common understanding of the underlying business document semantics and most existing approaches are not able to cope with the huge variety of business document formats, stemming from highly diverse requirements of the different stakeholders. Developed and applied in the course of the EU-funded research project GENESIS, this paper presents a comprehensive core component-based business document modelling approach that builds upon existing standards such as the OASIS Universal Business Language (UBL) and the UN/CEFACT Core Component Technical Specification (CCTS). These standards are extended by introducing the concept of generic business document templates out of which specific documents can be derived according to the actual user's needs. Key principle to achieve this flexibility is the integration of business context information that allows for modelling standard-based but at the same time customized business documents. The resulting modelling framework ranges from (tool-supported) graphical data models to the technical representation of the business documents as XML schema documents designed in compliance with the UN/CEFACT XML schema Naming and Design Rules (NDR). [PUBLICATION ABSTRACT]

This chapter contains sections titled: Transport Layer Integration in the AutoNet Generic Reference Protocol Stack TCP in AutoNets Summary References