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The intracellular protozoan parasite of the genus Cryptosporidium is among the leading causes of waterborne diarrheal disease outbreaks throughout the world. The parasite is transmitted by ingestion of infective oocysts that are highly stable in the environment and resistant to almost all conventional disinfection methods and water treatments. Control of the parasite infection is exceedingly difficult due to the excretion of large numbers of oocysts in the feces of infected individuals that contaminate the environment and serve as a source of infection for susceptible hosts including humans and animals. Drug development against the parasite is challenging owing to its limited genetic tractability, absence of conventional drug targets, unique intracellular location within the host, and the paucity of robust cell culture platforms for continuous parasite propagation. Despite the high prevalence of the parasite, the only US Food and Drug Administration (FDA)-approved treatment of Cryptosporidium infections is nitazoxanide, which has shown moderate efficacy in immunocompetent patients. More importantly, no effective therapeutic drugs are available for treating severe, potentially life-threatening cryptosporidiosis in immunodeficient patients, young children, and neonatal livestock. Thus, safe, inexpensive, and efficacious drugs are urgently required to reduce the ever-increasing global cryptosporidiosis burden especially in low-resource countries. Several compounds have been tested for both in vitro and in vivo efficacy against the disease. However, to date, only a few experimental compounds have been subjected to clinical trials in natural hosts, and among those none have proven efficacious. This review provides an overview of the past and present anti- Cryptosporidium pharmacotherapy in humans and agricultural animals. Herein, we also highlight the progress made in the field over the last few years and discuss the different strategies employed for discovery and development of effective prospective treatments for cryptosporidiosis.

Semantic code clone detection plays an essential role in software maintenance and quality assurance, as it helps uncover fragments of code that express the same logic even when their syntax has been altered or deliberately obfuscated. In this study, we propose a framework that combines hybrid representation learning with deep bidirectional LSTM networks. The model is applied to two intermediate forms of Java programs—Baf and Jimple—extracted through the Soot framework, which together provide both syntactic structure and semantic detail. This design allows the method to cope with difficult obfuscation strategies such as polymorphism and metamorphism. In our experiments, the framework showed strong and stable performance. Training accuracy reached about 98%, while validation accuracy stayed above 95%, with good generalization across the different clone categories described in the Twilight-Zone taxonomy. When compared with other recurrent models, the BiLSTM consistently performed better, especially when combined with multiple intermediate representations and attention mechanisms. On the BigCloneBench dataset, the approach matched or exceeded the results of state-of-the-art tools, achieving recall and F1-scores of up to 97% on challenging clone types. These findings confirm the practical applicability of hybrid intermediate representations for semantic clone detection and suggest promising directions for future research using transformer-based models and large-scale deployment.

The circular restricted three-body problem (CR3BP) is a fundamental model in celestial mechanics, typically formulated under the assumption that the gravitational fields of the primaries are time independent in the rotating frame. In realistic systems, however, celestial bodies may exhibit rotational variability, particularly when their mass distribution deviates from spherical symmetry, leading to a time-dependent reorientation of the gravitational field. In this work, we extend the classical CR3BP by incorporating a small periodic rotational wobble of the smaller primary, modeled as a first-order perturbation associated with orientation-dependent gravitational effects. The resulting system becomes non-autonomous, and classical libration points are replaced by time-dependent equilibrium configurations whose displacement is obtained analytically using perturbation theory. Linear stability is investigated using Floquet analysis, revealing systematic but moderate modifications to the stability spectrum near libration regions. Numerical simulations for the Sun-Earth system demonstrate that rotational wobble induces persistent modulation of motion and leads to cumulative trajectory deviations over time. When expressed in physical units, these deviations can reach magnitudes of 10 -10  m over extended integration periods, depending on the wobble amplitude. These results highlight rotational wobble as a physically motivated mechanism for introducing time-dependent perturbations into restricted three-body dynamics, with potential implications for high-precision trajectory modeling and long-duration space missions.

Self-reported measures of height and weight are often used in large epidemiological studies. However, concerns remain regarding the validity and reliability of these self-reported measures. The aim of this systematic review was to summarise and evaluate the comparative validity of measured and self-reported weight and height data and to recommend strategies to improve the reliability of self-reported-data collection across studies. This systematic review adopted the PRISMA guidelines. Four online sources, including PubMed, Medline, Google Scholar, and CINAHL, were utilised. A total of 17,800 articles were screened, and 10 studies were eligible to be included in the SLR based on the defined inclusion and exclusion criteria. The findings from the studies revealed good agreement between measured and self-reported weight and height based on intra-class correlation coefficient and Bland–Altman plots. Overall, measured weight and height had higher validity and reliability (ICC > 0.9; LOA < 1 SD). However, due to biases such as social pressure and self-esteem issues, women underreported their weight, while men overreported their height. In essence, self-reported measures remain valuable indicators to supplement the restricted direct anthropometric data, particularly in large-scale surveys. However, it is essential to address potential sources of bias.

In medical imaging, the detection and classification of stomach diseases are challenging due to the resemblance of different symptoms, image contrast, and complex background. Computer-aided diagnosis (CAD) plays a vital role in the medical imaging field, allowing accurate results to be obtained in minimal time. This article proposes a new hybrid method to detect and classify stomach diseases using endoscopy videos. The proposed methodology comprises seven significant steps: data acquisition, preprocessing of data, transfer learning of deep models, feature extraction, feature selection, hybridization, and classification. We selected two different CNN models (VGG19 and Alexnet) to extract features. We applied transfer learning techniques before using them as feature extractors. We used a genetic algorithm (GA) in feature selection, due to its adaptive nature. We fused selected features of both models using a serial-based approach. Finally, the best features were provided to multiple machine learning classifiers for detection and classification. The proposed approach was evaluated on a personally collected dataset of five classes, including gastritis, ulcer, esophagitis, bleeding, and healthy. We observed that the proposed technique performed superbly on Cubic SVM with 99.8% accuracy. For the authenticity of the proposed technique, we considered these statistical measures: classification accuracy, recall, precision, False Negative Rate (FNR), Area Under the Curve (AUC), and time. In addition, we provided a fair state-of-the-art comparison of our proposed technique with existing techniques that proves its worthiness.

In this paper, the circular Sitnikov three-body problem is studied under the combined influence of radiation pressure and albedo. The model consists of two equal-mass primaries moving in circular orbits about their center of mass and an infinitesimal body constrained to oscillate along the perpendicular axis. The radiative emission from one primary and the reflected radiation from the other are incorporated into the effective potential through radiation and reflectivity parameters. Using the Jacobi integral, we determine the energetically admissible region for vertical motion and examine how radiative effects modify the accessible phase space. The study shows that the system admits a single vertical equilibrium point at the origin, which remains linearly stable within the physically admissible parameter range. Radiation and albedo reduce the effective restoring force and increase the oscillation period, producing a measurable rescaling of the physical time without altering the geometrical structure of the phase trajectories. The phase-space dynamics are further explored by means of Poincare (first-return) maps obtained from numerical integration of the nonlinear equation of motion. The resulting invariant curves confirm that the motion remains regular and bounded, while their progressive contraction reflects the reduction in the oscillation amplitude with increasing radiative effects. Overall, the results show that albedo acts as a quantitative modifier of the vertical Sitnikov dynamics by changing the effective potential, the admissible energy domain, and the observable time scale, without generating new qualitative phase-space structures.

Filarial nematodes cause severe illnesses in humans and canines including limb deformities and disfigurement, heart failure, blindness, and death, among others. There are no vaccines, and current drugs against filarial nematodes infections have only modest effects and are prone to complications. We identified a gene (herein called DiMT) encoding an S-adenosyl-L-methionine (SAM)-dependent methyltransferase with orthologs in parasite filarial worms but not in mammals. By in silico analysis, DiMT possesses catalytic sites for binding SAM and catecholamines with high affinity. We expressed and purified recombinant DiMT protein and used it as an enzyme in a series of SAM-dependent methylation assays. DiMT acted specifically as a catechol-O-methyltransferase (COMT), catalyzing catabolic methylation of dopamine, and depicted Michaelis Menten kinetics on substrate and co-substrate. Among a set of SAM-dependent methyltransferase inhibitors, we identified compounds that bound with high affinity to DiMT's catalytic sites and inhibited its enzymatic activity. By testing the efficacy of DiMT inhibitors against microfilariae of Dirofilaria immitis in culture, we identified three inhibitors with concentration- and time-dependent effect of killing D. immitis microfilariae. Importantly, RNAi silencing of a DiMT ortholog in Caenorhabditis elegans has been shown to be lethal, likely as a result of excessive accumulation of active catecholamines that inhibit worm locomotion, pharyngeal pumping and fecundity. Together, we have unveiled DiMT as an essential COMT that is conserved in parasitic filarial nematodes, but is significantly different from mammalian COMTs and, therefore, is a viable target for development of novel drugs against filarial nematode infections.

Green energy production from natural resources can reduce emissions of greenhouse gases and pollutants from burning of fossil fuels in power plants. Recently, groundwater geothermal energy (GGE) is harnessed by deploying closed- and open-loop heat systems. In open-loop geothermal heat pump systems (OLGHPS), groundwater is reinjected into aquifer after harnessing GGE. Nevertheless, OLGHPS face noxious clogging issue because of elusive chemistry (corrosion or precipitation) of chemical species, principally of iron (Fe), in pipes and aquifers during reinjection process via oxidation reactions. Plethora of filtering materials are available for removal of ions, but these are quite expensive and environmentally unsafe. More recently, low-cost, eco-friendly, green filtering materials gain much interest. These materials can remove ions from groundwater that can minimize clogging in heat exchange systems, injection wells, and aquifer. In the present study, three filtering materials, i.e., wooden charcoal (biomaterial), yamazuna fine sand, and volcanic ash, were tested to estimate their Fe removal capacity. In upward flow mode with minimum oxygen-water contact, serial column (each with 6 ports) experiments were conducted under constant pressure head and constant velocity conditions. Columns were connected to well water having dissolved Fe concentration of 10.85 mg L−1. Sampling was done at the well, column inlets, column’s six sampling ports and column outlets, and samples were analyzed for Fe by atomic absorption spectroscopy. Related tested parameters include pH, EC, temperature, turbidity, porosity, particle diameter, and dissolved oxygen. Volcanic ash showed less Fe removal, while sand filter showed substantial reduction in velocity. Biomaterial (wooden charcoal) displayed higher Fe adsorption capacity compared to other materials that can be ascribed to its surface chemistry and functional groups. Under different flow rates, maximum Fe content of 3.5 g Fe kg−1 dry charcoal was obtained. By considering a safety factor and influence of groundwater composition, it is possible to design a biomaterial-based iron filter system to minimize Fe-induced chemical clogging in OLGHPS which is an eco-friendly, green energy source.

This research explores the impact of reflected radiation on the formation and stability of out-of-plane equilibrium points within the circular restricted three-body problem (CR3BP). The study focuses on scenarios where the larger primary body emits radiation and the smaller primary reflects this radiation. Three distinct cases are analyzed based on the parameter λ, representing the ratio of radial distances from the infinitesimal mass to the primary bodies. The research identifies the conditions under which symmetric out-of-plane equilibrium points can form and evaluates their stability, concluding that these points are linearly unstable in all examined cases. The study also delves into the nature of orbits surrounding these equilibrium points, offering important insights into the complex dynamics influenced by albedo effects. Finally, the findings of this study are applied to a realistic model, specifically the Sun-Earth-Satellite system, to validate the theoretical analysis. Upon applying the theoretical framework to the Sun–Earth–Satellite system, we confirm that out-of-plane equilibrium points do not exist in this real planetary setup. This result is consistent with the derived mathematical conditions, showing that the gravitational and dynamical constraints of the system inhibit the formation of such equilibria. Here, the parameter λ plays a key role in classifying the possible configurations. These insights contribute to a more comprehensive understanding of equilibrium structures in celestial mechanics and their implications for planetary motion and space dynamics.

Background Herbs and spices are very important and useful as therapeutic agent against many pathological infections. Increasing multidrug resistance of pathogens forces to find alternative compounds for treatment of infectious diseases. Methods In the present study the antimicrobial potency of garlic and ginger has been investigated against eight local clinical bacterial isolates. Three types of extracts of each garlic and ginger including aqueous extract, methanol extract and ethanol extract had been assayed separately against drug resistant Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumoniae, Shigella sonnei, Staphylococcus epidermidis and Salmonella typhi. The antibacterial activity was determined by disc diffusion method. Results All tested bacterial strains were most susceptible to the garlic aqueous extract and showed poor susceptibility to the ginger aqueous extract. The (minimum inhibitory concentration) MIC of different bacterial species varied from 0.05 mg/ml to 1.0 mg/ml. Conclusion In the light of several socioeconomic factors of Pakistan mainly poverty and poor hygienic condition, present study encourages the use of spices as alternative or supplementary medicine to reduce the burden of high cost, side effects and progressively increasing drug resistance of pathogens.