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Many platinum group metal-free inorganic catalysts have demonstrated high intrinsic activity for diverse important electrode reactions, but their practical use often suffers from undesirable structural degradation and hence poor stability, especially in acidic media. We report here an alkali-heating synthesis to achieve phase-mixed cobalt diselenide material with nearly homogeneous distribution of cubic and orthorhombic phases. Using water electroreduction as a model reaction, we observe that the phase-mixed cobalt diselenide reaches the current density of 10 milliamperes per square centimeter at overpotential of mere 124 millivolts in acidic electrolyte. The catalyst shows no sign of deactivation after more than 400 h of continuous operation and the polarization curve is well retained after 50,000 potential cycles. Experimental and computational investigations uncover a boosted covalency between Co and Se atoms resulting from the phase mixture, which substantially enhances the lattice robustness and thereby the material stability. The findings provide promising design strategy for long-lived catalysts in acid through crystal phase engineering. Noble-metal-free catalysts often show stability issues in acidic media due to structural degradation. Here authors show that phase-mixed engineering of cobalt diselenide electrocatalysts can enable greater covalency of Co-Se bonds and improve robustness for catalyzing hydrogen evolution in acid.

With the rapid development of Internet media, people have a variety of ways to obtain information. Special children are coming into the public’s vision, and the life and learning of many visually impaired children and autistic children are also attracting more and more people’s attention, which also enables more people to truly understand these special worlds. Whether these visually impaired children and autistic children are included in ordinary teachers has also become a hot topic. Based on real cases in reality, some blind people receive general education, which is more conducive to their future development. Some celebrity writers, etc., also realize their life value. By collecting the academic performance and psychological emotions of visually impaired and autistic children, this paper analyses prior studies which conduct surveys and test questionnaire data. The present study also makes a comparative analysis of the data, to explore whether visually impaired children and autistic children should receive general education. The result reveals that blind and autistic children need to receive formal education to develop social and learning skills.

Background Mitochondrial dynamics is the result of a dynamic balance between fusion and fission events, which are driven via a set of mitochondria-shaping proteins. These proteins are generally considered to be binary components of either the fission or fusion machinery, but potential crosstalk between the fission and fusion machineries remains less explored. In the present work, we analyzed the roles of mitochondrial elongation factors 1 and 2 (MIEF1/2), core components of the fission machinery in mammals. Results We show that MIEFs (MIEF1/2), besides their action in the fission machinery, regulate mitochondrial fusion through direct interaction with the fusion proteins Mfn1 and Mfn2, suggesting that MIEFs participate in not only fission but also fusion. Elevated levels of MIEFs enhance mitochondrial fusion in an Mfn1/2- and OPA1-dependent but Drp1-independent manner. Moreover, mitochondrial localization and self-association of MIEFs are crucial for their fusion-promoting ability. In addition, we show that MIEF1/2 can competitively decrease the interaction of hFis1 with Mfn1 and Mfn2, alleviating hFis1-induced mitochondrial fragmentation and contributing to mitochondrial fusion. Conclusions Our study suggests that MIEFs serve as a central hub that interacts with and regulates both the fission and fusion machineries, which uncovers a novel mechanism for balancing these opposing forces of mitochondrial dynamics in mammals.

Mitochondrial dynamics is a fundamental cellular process and recruitment of Drp1 to mitochondria is an essential step in mitochondrial fission. Mff and MIEF1/2 (MiD51/49) serve as key receptors for recruitment of Drp1 to mitochondria in mammals. However, if and how these receptors work together in mitochondrial fission is poorly understood. Here we show that MIEFs interact with both Drp1 and Mff on the mitochondrial surface and serve as adaptors linking Drp1 and Mff together in a trimeric Drp1-MIEF-Mff complex. Thus, MIEFs can regulate the interaction between Drp1 and Mff, and also Mff-induced Drp1 accumulation on mitochondria. It is shown that loss of endogenous MIEFs severely impairs these processes. Additionally, in cells depleted of endogenous MIEF1/2, high levels of exogenous MIEFs sequester Drp1 on the mitochondrial surface, resulting in mitochondrial elongation, whereas low-to-moderate levels of MIEFs promote mitochondrial fission, leading to mitochondrial fragmentation. In sum, the data suggest that MIEFs and Mff work coordinately in Drp1-mediated mitochondrial fission and that the level of MIEF1/2 relative to Mff sets the balance between mitochondrial fission and fusion.

Sugarcane is a multifunctional crop mainly used for sugar and renewable bioenergy production. Accurate and timely estimation of the sugarcane yield before harvest plays a particularly important role in the management of agroecosystems. The rapid development of remote sensing technologies, especially Light Detecting and Ranging (LiDAR), significantly enhances aboveground fresh weight (AFW) estimations. In our study, we evaluated the capability of LiDAR mounted on an Unmanned Aerial Vehicle (UAV) in estimating the sugarcane AFW in Fusui county, Chongzuo city of Guangxi province, China. We measured the height and the fresh weight of sugarcane plants in 105 sampling plots, and eight variables were extracted from the field-based measurements. Six regression algorithms were used to build the sugarcane AFW model: multiple linear regression (MLR), stepwise multiple regression (SMR), generalized linear model (GLM), generalized boosted model (GBM), kernel-based regularized least squares (KRLS), and random forest regression (RFR). The results demonstrate that RFR (R2 = 0.96, RMSE = 1.27 kg m−2) performs better than other models in terms of prediction accuracy. The final fitted sugarcane AFW distribution maps exhibited good agreement with the observed values (R2 = 0.97, RMSE = 1.33 kg m−2). Canopy cover, the distance to the road, and tillage methods all have an impact on sugarcane AFW. Our study provides guidance for calculating the optimum planting density, reducing the negative impact of human activities, and selecting suitable tillage methods in actual cultivation and production.

Non-invasive optical imaging of neuronal voltage response signals in live brains is constrained in depth by the optical diffusion limit, which is due primarily to optical scattering by brain tissues. Although photoacoustic tomography breaks this limit by exciting the targets with diffused photons and detecting the resulting acoustic responses, it has not been demonstrated as a modality for imaging voltage responses. In this communication, we report the first demonstration of photoacoustic voltage response imaging in both in vitro HEK-293 cell cultures and in vivo mouse brain surfaces. Using spectroscopic photoacoustic tomography at isosbestic wavelengths, we can separate voltage response signals and hemodynamic signals on live brain surfaces. By imaging HEK-293 cell clusters through 4.5 mm thick ex vivo rat brain tissue, we demonstrate photoacoustic tomography of cell membrane voltage responses beyond the optical diffusion limit. Although the current voltage dye does not immediately allow in vivo deep brain voltage response imaging, we believe our method opens up a feasible technical path for deep brain studies in the future.

The role of the voltage‐gated sodium channel 1.7 (Nav1.7) is unclear in models of neuropathic pain induced by nerve injury. In the present study, we measured expression levels of Nav1.7 in two distinct neuropathic pain models: spinal nerve ligation (SNL) and chronic constriction injury (CCI). In the SNL model, both mRNA and protein levels of Nav1.7 were markedly lower in the L5 dorsal root ganglia (DRG) but were significantly higher in the L4 DRG. Nav1.7 protein levels were notably higher in both L4 and L5 DRGs under CCI conditions. We found that excessive damage of L5 nerves such as SNL reduced expression levels of Nav1.7 in the injured L5 DRG and activated the adjacent uninjured DRG, resulting in Nav1.7 level increases in the adjacent L4 DRG. We confirmed again that Nav1.7 was closely related to neuropathic pain induced by nerve injury. More importantly, our results suggest that tracing the molecular changes exclusively in the L5 DRG in SNL model may not completely explain the pain mechanism; it is necessary to study the adjacent uninjured L4 DRG.

Osteoarthritis (OA) is the most common chronic joint disease worldwide. Chondrocyte, as the only resident cell type in cartilage, its apoptosis is of pathogenetic significance in OA. Mesenchymal stem cell (MSC)-based-therapy has been proved effective in OA in animals and clinical studies. Nowadays, the regenerative potential of MSC-based therapy is mostly attributed to its paracrine secretion, in which exosomes may play an important role. In the present study, we aimed to find out the significance of MSC-derived exosomes (MSC-Exos) on the viability of chondrocytes under normal and inflammatory conditions. Bone marrow MSCs (BMSCs) and chondrocytes from rabbits were cultured in vitro. BMSC-Exos were isolated by an ultracentrifugation method. Transmission electron microscopy and Western blot were used to identify exosomes. The internalization of BMSC-Exos into chondrocytes was observed by fluorescent microscope. The viability and apoptosis of chondrocytes induced by IL-1β were tested through MTT method, Hoechst33324 dying, and mitochondrial damage measurement. Phosphorylation of p38, ERK, and Akt were evaluated by Western blot. The results showed that BMSC-Exos were round-shaped. Co-culturing BMSC-Exos with chondrocytes could observe the uptake of BMSC-Exos by chondrocytes. The viability decreased, apoptosis occurred, and the mitochondrial membrane potential of chondrocytes changed a lot when IL-1β were given, but all the changes were almost abolished when BMSC-Exos was added. Furthermore, the phosphorylation of p38 and ERK were inhibited, and phosphorylation of Akt was promoted by BMSC-Exos compared with IL-1β group. The present study demonstrated that BMSC-Exos inhibited mitochondrial-induced apoptosis in response to IL-1β, and p38, ERK, and Akt pathways were involved. BMSC-Exo might represent a novel cell-free therapeutic approach for the treatment of OA.

Osteoporosis (OP), a common systemic metabolic bone disease, is characterized by low bone mass, increasing bone fragility and a high risk of fracture. At present, the clinical treatment of OP mainly involves anti-bone resorption drugs and anabolic agents for bone, but their long-term use can cause serious side effects. The development of stem cell therapy and regenerative medicine has provided a new approach to the clinical treatment of various diseases, even with a hope for cure. Recently, the therapeutic advantages of the therapy have been shown for a variety of orthopedic diseases. However, these stem cell-based researches are currently limited to animal models; the uncertainty regarding the post-transplantation fate of stem cells and their safety in recipients has largely restricted the development of human clinical trials. Nevertheless, the feasibility of mesenchymal stem cells to treat osteoporotic mice has drawn a growing amount of intriguing attention from clinicians to its potential of applying the stem cell-based therapy as a new therapeutic approach to OP in the future clinic. In the current review, therefore, we explored the potential use of mesenchymal stem cells in human OP treatment.

A review of the global history of tourism development reveals a strong connection between government involvement and the growth of the tourism industry. In China, tourism authorities are increasingly emphasizing the role of government service websites in advancing digital governance within the sector. Drawing on theories of public administration, resource dependence, and regional innovation systems, this study constructs an analytical framework to investigate the influencing mechanisms of government service websites on tourism development. Using data from the government tourism websites of 31 Chinese provinces, this study systematically examines, for the first time, the mediating role of tourism resources and the moderating effect of provincial economic competitiveness. Content analysis and keyword extraction techniques were employed to collect data on website service functions, tourism resources, and tourism development for the year 2023. These were analyzed using a PLS structural equation model. The results indicate that government website service functions positively, albeit modestly, promote tourism development, explaining 24.4% of the variance. Among service functions, public services (weight = 0.611) contribute significantly more than administrative management (0.368) and information management (0.238). Although the mediating effect of tourism resources did not reach statistical significance, internal structural analysis shows that cultural customs resources (0.512) contribute more to tourism development than water landscapes (0.443) and geological landscapes (0.338), highlighting the unique advantage of “soft resources” in a digital environment. Incorporating provincial economic competitiveness significantly increases the explanatory power of the model to 47.9%, with development level competitiveness (0.268) and government function competitiveness (0.267) playing the most prominent moderating roles. These findings suggest that the effectiveness of digital governance in promoting tourism development is shaped by multiple complex factors. Accordingly, governments should adopt differentiated digital strategies based on regional economic conditions and resource endowments, with a focus on enhancing public service functions, optimizing the digital representation of cultural resources, and strengthening regional economic foundations to fully unleash the industrial potential of digital governance.