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Agrotis ipsilon (Lepidoptera: Noctuidae) is a major underground pest that damages many agricultural crops in China and other countries. A diet-incorporation-based bioassay was conducted to evaluate the sublethal effects of the novel anthranilic diamide chlorantraniliprole on the nutritional physiology, enzymatic properties and population parameters of this cutworm. Chlorantraniliprole exhibited signs of active toxicity against third instar larvae of A . ipsilon , and the LC 50 was 0.187 μg.g −1 of artificial diet after treatment for 72 h. The development time of the larval, pupal and adult stages was significantly affected after chlorantraniliprole exposure, compared to the control treatment. Relative to the control treatment, chlorantraniliprole decreased pupal and adult emergence rates, fecundity and fertility and increased the proportions of developmental deformities, the adult preoviposition period (APOP) and the total preoviposition period (TPOP). Furthermore, compared to those treated with the control, A . ipsilon larvae treated with low doses of chlorantraniliprole decreased food utilization and nutrient content (protein, lipid, carbohydrate, trehalose), showed lower pupal weights and growth rates. Compared with the control treatment, chlorantraniliprole significantly reduced digestive enzyme activities and observably increased detoxifying and protective enzyme activities and hormone titers. Importantly, these chlorantraniliprole-induced changes affected life table parameters of the cutworm. These results suggest that chlorantraniliprole at low concentrations can impair A . ipsilon development duration, normal food consumption and digestion process, enzymatic properties, hormone levels, fecundity and population levels. Chlorantraniliprole exhibit the potential to be exploited as a control strategy for this cutworm.

QYM201 is a 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibiting herbicide recently registered in China for controlling grass and broadleaf weeds in wheat. It is a novel herbicide, and its potential harm to soil ecosystems has not yet been reported. This study investigates the influence of QYM201 on soil enzyme activity and microorganism quantities in two different soils at concentrations of 0.1, 1, and 5 mg kg−1 soil. Results indicate that QYM201 initially inhibited soil protease, urease, and sucrase activity and this effect increased with concentration. During the later stages of incubation, inhibitory effects gradually weakened and by the end of the experiment (45 days), enzyme activity was restored to control levels. Catalase activity was stimulated by QYM201, with significant differences observed between the QYM201-treated groups and the control at the onset of exposure. This stimulation effect decreased during the later stages of the experiment. However, catalase activity was still significantly higher at the end of the experiment compared to the control. The effects of QYM201 on soil microorganisms differed. Initially, bacteria and actinomycetes quantities were decreased by QYM201 (10 days). As the incubation progressed, microorganism quantities in the lower concentration groups (0.1 and 1 mg kg−1 soil) were restored to control levels, while those of the high concentration group (5 mg kg−1 soil) did not fully recover. QYM201 did not significantly impact the quantity of fungi. The half-life and degradation rate constant (k) of QYM201 for the two studied soil types were 23.1 days and 16.1 days, and 0.030 and 0.043 day−1, respectively.

Effective intervention on oxidative damage of bovine mammary epithelial cells (bMECs) is particularly important for reducing the incidence rate of mastitis. As a natural antioxidant, resveratrol (RES) can scavenge ROS, protecting cells from oxidative damage. However, the role of RES in bMECs and its potential protective mechanism have not been fully elucidated. Our results indicated that RES alleviated oxidative damage and enhanced antioxidant capacity in bMECs. Furthermore, RES increased SIRT5 expression and interacted with SIRT5, which attenuated cellular oxidative stress, inflammatory response and autophagy activity. Interestingly, SIRT5 and RES further improved mitochondrial dysfunction by increasing intracellular NADPH and GSH levels. Meanwhile, RES activated SIRT5 to regulate enzymatic activity of SDH and IDH2, which were important enzymes for producing intracellular ATP and antioxidants. RES specifically activated SIRT5 to attenuate the succinylation levels of intracellular IDH2 associated with interacting with SIRT5. Collectively, these outcomes revealed that RES might function as an activator of SIRT5 to attenuate oxidative damage of bMECs via activating SIRT5-IDH2 axis, resulting in increased GSH and NADPH production. Therefore, RES may be useful to prevent and control bovine mastitis by relieving oxidative damage.

In recent years, banded leaf sheath blight in maize (Zea mays L.) has become an important disease that seriously affects quality and yield. This paper aims to evaluate the sensitivity of Rhizoctonia solani Kuhn to thifluzamide on maize, to clarify the effect of seed coating using a thifluzamide suspension agent on safety and physiological indicators and to determine the effectiveness of control of banded leaf sheath blight in the field. In this study, the thifluzamide sensitivity of 102 strains of R. solani in maize from Shandong was determined using the mycelial growth rate method; the average half-maximal effective concentration value (EC50) was 0.086 ± 0.004 μg/ml and displayed a unimodal frequency distribution, indicating that thifluzamide had strong inhibitory activity on the mycelial growth of R. solani in maize. In an indoor pot test, the root activities under 24 g a.i./100 kg seed were found to increase by 78.01%, compared with the control. Similarly, chlorophyll content increased most significantly at this dose, by 32.3%. Thifluzamide (FS) could significantly increase the per-plot yield. Among the examined dosages, 48 g a.i./100 kg seed had the most significant treatment effect, with the yield rate increasing by 15.7% and 14.1%, respectively, in 2017 and 2018 compared with the control. The field effectiveness against banded leaf sheath blight in maize was highest at the dosage of 48 g a.i./100 kg seed for a seed dressing with thifluzamide (FS). These results indicate that thifluzamide has enormous potential for controlling banded leaf sheath blight in maize.

Heat stress (HS) has become one of the important factors affecting the development of animal husbandry. The purpose of this experiment was to investigate whether vitamin C (Vc) and betaine (Bet) improve immune organ index and humoral immunity by enhancing the antioxidant status of immune organs, thus protecting broilers from HS-induced injuries. A total of 200 28-day-old Ross 308 broilers were randomly assigned into 5 groups (n = 4 replicates/group, 10 broilers/replicate) which were reared at different ambient temperatures (24 ± 1°C or 33 ± 1°C). The control group fed basal diet, while high-temperature groups were either fed a basal diet (HS group) or a basal diet supplemented with 250-mg Vc/kg diet (HSVc group), 1000-mg Bet/kg diet (HSBet group), and 250-mg Vc plus 1000 mg Bet/kg diet (HSVcBet group), respectively. On day 42, growth performance, humoral immune function, immune organ index, and antioxidant capacity were measured. HS reduced the productive performance of broilers, antibody potency against the Newcastle disease virus (NDV) and sheep red blood cells (SRBC), indices of thymus and bursa, and antioxidant capacity of immune organs. Adding Vc alone or in combination with Bet improved performance, NDV and SRBC antibody potency, thymus and bursa indices, and antioxidant capacity of immune organs in heat-stressed broilers, with the most effective being combination. In summary, HS reduces the antioxidant capacity and immune organ development status of broiler immune organs. Vc and/or Bet can improve the development of immune organs and restore part of the production performance by regulating the antioxidant status of immune organs, among which the combined addition of Vc and Bet has the best effect.

This experiment investigated the sex responses of heat stress (HS) and subsequent recovery on growth performance, serum metabolic parameters, and redox status. Two hundred 38-day-old broilers were arranged in a completely randomized design with a 2 × 2 (temperatures and sexes) factorial arrangement in five replicates. Broilers were raised at 24 ± 1 °C or 32 ± 1 °C for 3 days and returned to 24 °C for 2 days. The study showed that HS decreased both average daily feed intake (ADFI), average daily gain (ADG), serum total glutathione peroxidase (GPx), and superoxide dismutase (T-SOD). However, it increased feed conversion ratio (FCR), rectal temperature (RT), respiratory rate (RR), serum glucose, blood urea nitrogen (BUN), low-density lipoprotein cholesterol, and the protein carbonyl group (PCG). Male broilers had higher ADFI, ADG, lactic acid (LA), high-density lipoprotein cholesterol (HDL-C), and PCG, but lower FCR, albumin, total antioxidant capacity, T-SOD, and GPx. Temperature and sex significantly interacted with ADFI, ADG, LA, and HDL-C. The effects of HS on RR, RT, glucose, albumin, BUN, PCG, T-SOD, and GPx recovered after 2 days. These results indicate that HS and subsequent recovery affect growth performance, which is accompanied by disturbances in serum nutrient metabolism and abnormalities in redox function and manifested by temporal and gender differences.

This study investigates the potential of vitamin C (VC) and/or betaine (Bet) to enhance growth performance, regulate serum metabolism, and bolster antioxidant function aiming to mitigate the impact of heat stress (HS) on broilers. Two hundred Ross 308 broilers at 28 days of age were randomly assigned to five groups. The control group, housed at 24 ± 1℃, was fed a basal diet. High-temperature treatment groups, housed at 32 ± 1℃, received a basal diet with 0 (HS group), 250 mg/kg VC (HSVC group), 1000 mg/kg Bet (HSBe group), and 250 mg/kg VC + 1000 mg/kg Bet (HSVCBe group). On day 42, assessments were made on growth performance, muscle quality, serum biochemistry, and antioxidant function. Results revealed that HS significantly lowered (P < 0.05) average daily feed intake (ADFI), the degree of redness (a*) in muscles, and serum total superoxide dismutase (T-SOD) level. It also reduced (P < 0.01) average daily gain (ADG), and serum total antioxidant capacity (T-AOC) level, while increasing (P < 0.05) shear force, serum direct bilirubin (D-BIL), uric acid (UA), and malondialdehyde (MDA) levels compared with the control group. Dietary supplementation of VC and Bet, either alone or in combination, significantly decreased shear force and serum UA level, while increasing ADG and serum T-AOC, T-SOD level compared with the HS group (P < 0.05). In conclusion, the addition of VC and/or Bet to the diet proves effective in enhancing the growth performance of HS-exposed broilers through the positive regulation of serum chemical metabolism and the alleviation of oxidative damage.

Heat stress (HS) can affect growth performance through alterations in specific gut microbiota, which greatly threatens poultry production. How HS affects the mechanisms of microbial changes in the poultry cecum and the complex interactions between cecal microbial changes and growth performance have not yet been well evaluated. This study was conducted to examine the changes in growth performance and cecal microbiotal community in cyclic heat stress (CHS)–treated broilers. A total of 200 twenty-eight-day-old female Arbor Acres (AA) broilers were equally allotted into neutral ambient temperature group (TN group, 24 ± 1°C, 24 h/day) and CHS group (33 ± 1°C, 8 h/day) with five replicates of 10 broilers each, respectively. Growth performance, cecum microbial diversity, flora composition, and community structure were analyzed on days 35 and 42. The decreased average daily feed intake (ADFI), average daily gain (ADG), and the increased feed/gain ratio (F:G) were observed in heat-stressed broilers on days 35 and 42. The alpha and beta diversity index had no significant changes at the two experimental periods ( P > 0.05). At the genus level, CHS significantly increased the relative abundance of Enterococcus at 42 days ( P < 0.05). Based on the analysis of linear effect size feature selection, CHS made an enriched Reyranella and a reduced Romboutsia and Ruminiclostridium at 35 days of age ( P < 0.05). CHS made an enriched Weissella and Enterococcus at 42 days of age ( P < 0.05). The present study revealed that CHS reduces broiler growth performance and alters the microbial community of the cecum microbiota and the abundance of species. These findings are of critical importance to alleviate the negative effects of CHS on broiler chickens’ growth performance by maintaining gut microbial balance.

[Purpose] Drought stress is an important abiotic factor that restricts the growth and yield of maize and poses a continuous threat to food security. The purpose of this study was to evaluate the protective effects of three different brassinolide types, 24-epibrassinolide, 28-homobrassinolide, and 28-epihombrassinolide, on the physiological, biochemical, and drought tolerance mechanisms of maize seedlings under two drought stresses. [Methods] The effects of different brassinolide isoforms on photosynthesis (chlorophyll content, photosynthetic parameters ( P n , G s , T r , and C i ), chlorophyll fluorescence parameters ( F v /F m , ΦPSII , qP , qN ), and the activity and gene expression of phosphoenolpyruvate carboxylase), antioxidants (superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and increase the content of proline), osmotic balance (malonaldehyde (MDA)), and stress tolerance gene expression ( ZmMYB48 gene) in maize seedlings after 7 days of drought stress were studied by spraying each kind of brassinolide at the appropriate concentration; the effects of brassinolide on maize yield were studied by simulating drought environment in field under drought shed. [Results] The results showed that the three brassinolides could enhance the photosynthetic performance of maize, induce the increase of antioxidant enzyme activity and proline content, and decrease MDA content; overexpression of drought tolerance gene ZmMYB48 was induced. Among them, 28-homobrassinolide at 0.03 mg·kg −1 had the best regulating activity; The field application of brassinolide can increase the yield of maize during harvest. Compared with the control, the yield of plots under severe drought stress increased by 27.67%, 42.26% and 34.22% respectively after 24-epibrassinolide, 28-homobrassinolide and 28-epihombrassinolide treatments. [Conclusions] These results provide a scientific basis for brassinolide application in maize under drought stress.

This study was aim to investigate the effects of lipoic acid (ALA) on performance, meat quality, serum biochemistry and antioxidant function of broilers under heat stress (HS). Two hundred1-day-old Cobb broilers were randomly divided into four treatment groups and each treatment consisted of 4 replicates of 10 broilers each. The treatment group adopts a 2 × 2 two-factor setting, which is divided into two diets (basic diet or 250 mg/kg ALA diet) and two temperatures (24 ± 1℃ or 33 ± 1℃). Starting from the 28th day of age, broilers are kept at room temperature (24 ± 1℃) or exposed to cyclic HS (33 ± 1℃ from 8:00 am to 18:00, 24 ± 1℃ from 18:00 to 8:00 am). HS significantly decreased serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), direct bilirubin (D-BIL), total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD). However, HS increased breast meat pH, serum uric acid (UA), total cholesterol (TC) and malondialdehyde (MDA). Dietary ALA significantly decreased leg meat shear force and water loss rate, serum total bilirubin(T-BIL), UA, triglyceride (TG) and MDA of broilers. However, it increased daily gain (ADG), serum D-BIL and T-SOD. There is a significant interaction between temperature and additives. HS can destroy the balance of the oxidation-antioxidant system, and lead to damage to liver and kidney function and confusion energy metabolism. Dietary ALA improves the antioxidant capacity of broilers, reduces the level of blood lipids and liver and kidney metabolism, and increases the daily gain of heat-stressed broilers.