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IntroductionAlthough teriparatide (TPTD) and exercise may improve osteoporosis, muscle atrophy, and fat metabolism during ageing, the effects of treatment with a combination of TPTD and exercise on these factors remain unclear. Therefore, this study examined the effects of TPTD and exercise on bone, skeletal muscle, and fat in ovariectomized and tail-suspended rats.Materials and methodsSeven-month-old female Wistar rats were ovariectomized and subjected to tail suspension. The rats were then randomized into one of the following four groups (n = 20/group) after 4 weeks: control group, treated with TPTD vehicle and no exercise; TPTD group (30 µg/kg TPTD, 3 days/week); Exercise group (treadmill at 12 m/min, 60 min/day, 5 days/week); and Combined group treated with TPTD and treadmill exercise. After 1 and 8 weeks of treatment, bone, skeletal muscle, and fat tissue parameters were evaluated.ResultsTPTD improved bone mineral density (BMD), bone structure, bone strength at the femoral metaphysis, and the percentage of skeletal muscle mass, and decreased the percentage of fat mass and the adipose volume in the bone marrow. Treadmill exercise increased BMD, bone strength of cancellous bone, and the percentage of skeletal muscle mass, and decreased the percentage of fat mass as seen on dual-energy X-ray absorptiometry. Furthermore, combined treatment significantly affected BMD, bone structure, and bone strength of cortical bone at the femoral diaphysis.ConclusionTPTD or treadmill exercise improved bone, skeletal muscle, and fat mass. Combination therapy with TPTD and exercise had synergistic effects on BMD, structure, and bone strength in ovariectomized, tail-suspended rats.

Surface-enhanced Raman scattering (SERS) spectroscopy is an ultra-sensitive analytical method with the powerful signal-molecule detection capability. Coupling with the polydimethylsiloxane (PDMS) material, SERS can be enabled on a polymeric substrate for fast-developing bio-compatible sensing applications. However, due to PDMS’s high viscosity, conventional PDMS-SERS substrates are typically thick and stiff, limiting their freedom for engineering flexible micro/nano functioning devices. To address this issue, we propose to adopt a low viscosity decamethylcyclopentasiloxane (D5) solvent as a diluent solution. Via controlling the mixture ratio of D5 and PDMS and the spin-coating speed for deposition, this method resulted in a film of a well-defined thickness from sub-millimeter down to a 100 nm scale. Furthermore, thanks to the unsaturated Si-H chemical bonds in the PDMS curing agent, the PDMS film could effectively reduce the Ag+ ions to Ag nanoparticles (NPs) directly bonding onto the substrate surface uniformly. Via adjusting the size and density of the AgNPs through reaction temperature and time, strong SERS was achieved and verified using R6G with the detection limit down to 0.1 ppm, attributed to the AgNPs’ plasmonic enhancement effect.

The cellular slime mould Dictyostelium discoideum is a soil-dwelling eukaryotic organism that undergoes distinctive morphological changes during starvation, making it a promising candidate for bioassay development. In this study, we evaluated the effects of copper (Cu) exposure on the morphological transformation of D. discoideum and performed a comparative proteomic analysis. Copper exposure on agar media delayed aggregate formation by 3.5 h compared to the controls. Approximately 280 protein spots were detected using immobilised pH gradient two-dimensional gel electrophoresis followed by silver staining. Three spots disappeared upon exposure to Cu. Based on isoelectric point and molecular weight analyses, the proteins were predicted to be formin-1, a cytoplasmic regulator of adenylyl cyclase (CRAC), and a tetratricopeptide repeat (TPR)-containing protein. Formin-1 and CRAC are involved in aggregation processes. These findings suggest that Cu disrupts aggregation-related protein expression in D. discoideum and highlight the potential of D. discoideum-based bioassays using proteomic biomarkers for environmental monitoring.

Titanium-nickel alloy is an attractive material due to its unique properties of shape memory effect, superior elasticity, and biocompatibility. Generally, Ti-Ni alloy powders are prepared from pure elemental powders of Ti and Ni as starting materials, but it is an energy-intensive process to obtain pure titanium. In this study, intermetallic compound TiNi powder passivated by TiOx shell was prepared by directly reducing a commercial NiTiO3 using CaH2 reducing agent in a molten LiCl at 650 °C. Analyses by X-ray diffraction, scanning electron microscopy/transmission electron microscopy with energy-dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy revealed that the powder had a core–shell structure, with the core of TiNi and the shell of TiOx-rich composition with scarce metallic Ni nicely catalyzing hydrogenation reactions with good recyclability and stability.

IntroductionIn patients with type 2 diabetes mellitus (T2DM), bone fragility increases fracture risk. Teriparatide (TPTD) improves bone strength, and exercise therapy suppresses blood glucose levels in T2DM. In this study, the combined effects of TPTD and exercise therapy on trabecular and cortical bone were examined in advanced T2DM model rats.Materials and methodsThirty-week-old Otsuka Long–Evans Tokushima Fatty rats were divided into four groups (n = 9–10 in each group at two time points): Cont group (vehicle-treated control), TPTD group (TPTD 30 μg/kg injected subcutaneously, 3 times/week), Exe group (treadmill exercise, 10 m/min, 60 min/day, 5 times/week), and Comb group (TPTD-treated and treadmill exercise combined). Five and 10 weeks after treatment, bone mineral density (BMD), bone strength, and bone micro-architecture were measured.ResultsTPTD and combined treatment significantly increased BMDs of the lumbar spine and femur compared to the Cont group (p < 0.05 to p < 0.01). In the three-point bending test of the femur, only combined treatment increased the maximum load at 5 weeks compared with the Cont and Exe groups (p < 0.01). In the compression test of the distal femoral metaphysis, both TPTD and combined treatment increased the trabecular bone strength compared with the Cont and Exe groups (p < 0.05 to p < 0.01). Although TPTD and combined treatment improved the micro-architecture of trabecular bone (p < 0.05 to p < 0.01), only combined treatment improved the micro-structures of cortical bone from 5 weeks of treatment (p < 0.05 to p < 0.01).ConclusionThe combination of TPTD and treadmill exercise increased BMD and trabecular and cortical bone strength of the femur with improved micro-architecture in T2DM model rats.

IntroductionThe increasing prevalence of osteoporosis and chronic kidney disease (CKD) due to the aging of society has highlighted the need for development of effective treatments for elderly patients. This study examined whether the combination of treadmill exercise therapy and alendronate (ALN) can improve bone mineral density (BMD) and bone strength without worsening renal function in adenine-induced CKD model rats.Materials and methods8-week-old male Wistar rats (n = 70) were divided into experimental groups based on the treatment protocol, i.e., non-CKD (control), vehicle only (CKD), ALN only, exercise only, and combined ALN plus exercise. A 0.75% adenine diet was used to induce CKD. Groups were killed at either 20 or 30 weeks of age. Comprehensive assessments included serum and urine biochemistry tests, renal histology, bone histomorphometry, BMD measurement, micro-computed tomography examinations, and biomechanical testing.ResultsBlood biochemistry tests, urine analyses and histological evaluations of the kidney demonstrated that ALN treatment did not worsen renal function or kidney fibrosis in moderate-stage CKD model rats. Both ALN and treadmill exercise significantly suppressed bone resorption (p < 0.05–p < 0.01). Moreover, ALN monotherapy and combined ALN and treadmill exercise significantly improved BMD of the lumbar spine and femur, bone microstructure, and trabecular bone strength (p < 0.05–p < 0.01). Treadmill exercise was also shown to decrease cortical porosity at the mid-diaphysis of the femur and improve kidney fibrosis.ConclusionThe combination of ALN and treadmill exercise is effective in improving BMD, the microstructure of trabecular and cortical bone, and bone strength, without compromising renal function in adenine-induced CKD model rats.

High-entropy alloys (HEAs) have attracted a great deal of research interest these days because of their attractive properties. Low-temperature chemical synthesis methods are being developed to obtain nanoscale HEAs with low energy consumption. In this study, we prepared HEA Al0.2Co1.5CrFeNi1.5Ti0.5 nanoparticles from high-entropy oxide (HEO) (Al0.2Co1.5CrFeNi1.5Ti0.5)3O4 by a deoxidation process via a CaH2-assisted molten salt method at 600 °C. X-ray diffraction measurements demonstrated that the oxide precursor and the reduced product have single-phases of spinel structure and face-centered cubic structures, indicating the formation of HEO and HEA, respectively. The HEA nanoparticles exhibited superior catalytic performance in the liquid-phase hydrogenation of p-nitrophenol at room temperature with little leaching of the component elements. Scanning electron microscopy (SEM) with energy-dispersive X-ray spectrometry (EDX) exhibited a good distribution of constituent elements over the HEA nanoparticles in a micro-sized range. However, transmission electron microscopy (TEM) with EDX revealed a slight deviation of elemental distributions of Al and Ti from those of Co, Cr, Fe, and Ni in a nano-sized range, probably due to the incomplete reduction of aluminum and titanium oxides. The elemental homogeneity in the HEA nanoparticles could be improved by taking advantage of the HEO precursor with homogeneous elemental distributions, but the experimental results suggested the importance of the total reduction of oxide precursors to prepare homogeneous HEAs from HEOs.

In recent decades, nanoscale zero-valent iron (nZVI) has been extensively studied for application in environmental remediation because it is an eco-friendly, inexpensive nanomaterial with high reactivity. The chemical reduction of iron ions using NaBH4 in a liquid solution is the most frequently used method to obtain nZVI, but its drawbacks are the use of expensive and toxic NaBH4 and the secondary pollution caused by the B(OH)3 by-product. In this study, in order to obtain nZVI in a cleaner manner, we used a reduction method for Fe2O3 using CaH2, which is non-toxic and generates no pollutants. The results of X-ray diffraction, nitrogen adsorption, and scanning electron microscopy for the obtained samples indicated the formation of zero-valent iron nanopowder (22.5 m2/g) that was obtained via reduction at 220 °C for 5 h. The obtained nZVI was finally tested in the catalytic hydrogenation of p-nitrophenol as a model reaction of water remediation, verifying its good catalytic performance.

Zinc-included intermetallic compound catalysts of RhZn, PtZn, and PdZn with a molar ration of Zn/metal = 1/1, which are generally prepared using a hydrogen reduction approach, are known to show excellent catalytic performance in some selective hydrogenations of organic compounds. In this study, in order to reduce the incorporated mounts of the expensive noble metals, we attempted to prepare zinc-rich intermetallic compounds via a CaH2-assisted molten salt synthesis method with a stronger reduction capacity than the common hydrogen reduction method. X-ray diffraction results indicated the formation of RhZn13 and Pt3Zn10 in the samples prepared by the reduction of ZnO-supported metal precursors. In a hydrogenation reaction of p-nitrophenol to p-aminophenol, the ZnO-supported RhZn13 and Pt3Zn10 catalysts showed a higher selectivity than the RhZn/ZnO and PtZn/ZnO catalysts with the almost similar conversions. Thus, it was demonstrated that the zinc-rich intermetallic compounds of RhZn13 and Pt3Zn10 could be superior selective hydrogenation catalysts compared to the conventional intermetallic compound catalysts of RhZn and PtZn.

To report an instructive case involving destructive spondylitis and synovitis-acne-pustulosis-hyperostosis-osteitis (SAPHO) syndrome, presenting with torticollis and postoperative dysphagia without hoarseness, attributed to hidden myotonic dystrophy (DM). A 51-year-old male patient with a cervical deformity, who was previously managed conservatively for a metastatic tumor, underwent reconstruction surgery and subsequently experienced postoperative dysphagia. The presence of destructive spondylitis with torticollis, warranting prompt assessment to prevent paralysis, adds complexity to the delayed identification of DM. Given the rarity of DM, peculiar neurological symptoms and other systemic comorbidities did not lead to a preoperative diagnosis without prior knowledge. The patient's dysphagia induced respiratory arrest and required reintubation. Challenges in extubation and ventilator weaning arose due to hypercapnia. Superimposed COVID-19 infection elongated the duration of intubation. Extubation failed due to aspiration pneumonia and required a tracheotomy. Despite laryngeal elevation and preservation of the relaxation of the oesophageal entrance, the sensation and movement of the tracheopharynx were disturbed. The patient exhibited an oropharyngeal propulsive disorder, predominantly indicative of motor neuron disease. The patient's mother stated that his brother had been hospitalized for a long time after abdominal surgery. Finally, the patient was diagnosed with DM, which is known to cause post-anesthetic dysphagia. Recognizing the existence of severe destructive cervical spondylitis associated with SAPHO is crucial. Although DM is not very common, it is not classified as extremely rare. Therefore, surgeons should be mindful of the potential risks associated with general anesthesia in patients with DM. The complexity of preoperative conditions may hinder an accurate diagnosis. Recognizing and establishing preoperative expectations can assist surgeons in preventing complications, even if complex spinal surgery is required for patients with DM.