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Plasma turbulence plays a key role in space and astrophysical plasma systems, enabling the energy of magnetic fields and plasma flows to be transported to particle kinetic scales at which the turbulence dissipates and heats the plasma. Identifying the physical mechanisms responsible for the dissipation of the turbulent energy is a critical step in developing the predictive capability for the turbulent heating needed by global models. In this work, spacecraft measurements of the electromagnetic fields and ion velocity distributions by the Magnetospheric Multiscale (MMS) mission are used to generate velocity-space signatures that identify ion cyclotron damping in Earth’s turbulent magnetosheath, in agreement with analytical modeling. Furthermore, the rate of ion energization is directly quantified and combined with a previous analysis of the electron energization to identify the dominant channels of turbulent dissipation and determine the partitioning of energy among species in this interval. Most space plasmas are in turbulent state and turbulence plays an essential role in transferring energy from large to small scales. Here, the authors show direct measurements of ion cyclotron damping in the Earth’s turbulent magnetosheath plasma and the resulting ion and electron energization rates.

On 24 April 2023, a Coronal Mass Ejection event caused the solar wind to become sub‐Alfvénic, leading to the development of an Alfvén Wing configuration in the Earth's magnetosphere. Alfvén Wings have previously been observed as cavities of low flow around moons in Jupiter's and Saturn's magnetospheres, but the observing spacecraft did not have the ability to directly measure the Alfvén Wings' current structures. Through in situ measurements made by the Magnetospheric Multiscale spacecraft, the 24 April event provides us with the first direct measurements of current structures during an Alfvén Wing configuration. These structures are observed to be significantly more anti‐field‐aligned and electron‐driven than the typical diamagnetic magnetopause current, indicating the disruption caused to the magnetosphere current system by the Alfvén Wing formation. The magnetopause current is then observed to recover more of its typical, perpendicular structure during the magnetosphere's recovery from the Alfvén Wing formation. Plain Language Summary The solar wind applies pressure on the Earth's magnetic field, distorting it from a dipole into its compressed dayside and stretched tail configuration. However, this typical structure can be disrupted by eruptive solar events such as Coronal Mass Ejections (CMEs), which may cause the solar wind's pressure to drop low enough that it is no longer able to push the magnetosphere back to form a single unified tail. When this occurs, the tail splits into two separate structures, called Alfvén Wings. While this configuration is rare at Earth, it is common from interactions of the outer planets' magnetosphere's with their moons, where Alfvén Wing configurations have been studied and modeled. However, because the observing spacecraft lacked the necessary instrumentation, we have not yet directly observed the Alfvén Wing current structures. On 24 April 2023, a CME event led to the creation of an Alfvén Wing formation in the Earth's magnetosphere. We observed this event using the Magnetospheric Multiscale spacecraft, which enabled us to make the first direct observations of Alfvén Wing current structures. These currents were found to be mainly parallel to the local magnetic field, in contrast to typical magnetopause currents. Key Points On 24 April 2023, the Magnetospheric Multiscale (MMS) spacecraft observed an Alfvén Wing formation along the dawn‐flank of Earth's magnetosphere MMS's observations represent the first in situ measurements of Alfvén Wing current structures The current structures are found to be primarily anti‐field‐aligned, electron‐driven, and filamentary

We present the results of a multi‐event study of the normalized reconnection rate integrating events spanning the three primary regimes of reconnection observed by the Magnetospheric Multiscale mission. We utilize a new method for determining the normalized reconnection rate with fewer sources of uncertainty by estimating the aspect ratio and spatiotemporal variability of the current sheet with magnetic field gradients. After demonstrating this technique is valid in different regimes we perform a multi‐event analysis and find no dependence of reconnection rate on upstream conditions. The only statistically significant correlation is between spatiotemporal variability in the current sheet aspect ratio and an unsteady reconnecting component of the upstream magnetic field. This analysis suggests that under typical steady magnetospheric conditions the normalized reconnection rate is constant, which may be significant in predicting the terrestrial effects of space weather by providing insight into the efficiency of solar wind‐magnetospheric coupling.

The Vlasov equation describes collisionless plasmas in the continuum limit and applies to many fundamental plasma energization phenomena. Because this equation governs the evolution of plasma in six-dimensional phase space, studies of its structure have mostly been limited to numerical or analytical methods. Here terms of the Vlasov equation are determined from observations of electron phase-space density gradients measured by the four Magnetospheric Multiscale spacecraft in the vicinity of magnetic reconnection at Earth’s magnetopause. We identify which electrons in velocity space substantially support the electron pressure divergence within electron-scale current layers. Furthermore, we isolate and characterize the effects of density, velocity and temperature gradients on the velocity-space structure and dynamics of these electrons. Unipolar, bipolar and ring structures in the electron phase-space density gradients are compared to a simplified Maxwellian model and correspond to localized gradients in density, velocity and temperature, respectively. These structures have implications for the ability of collisionless plasmas to maintain kinetic Vlasov equilibrium. The results provide a kinetic perspective relevant to how the electron pressure divergence may develop to violate the electron frozen-in condition and sustain electron-scale energy conversion processes, such as the reconnection electric field, in collisionless space plasma environments.Insights into the structure of the Vlasov equation that governs the evolution of collisionless plasmas from observations have been limited. Now the spatial gradient term for electrons is analysed with recent data from the MMS mission.

There is ample evidence for magnetic reconnection in the solar system, but it is a nontrivial task to visualize, to determine the proper approaches and frames to study, and in turn to elucidate the physical processes at work in reconnection regions from in-situ measurements of plasma particles and electromagnetic fields. Here an overview is given of a variety of single- and multi-spacecraft data analysis techniques that are key to revealing the context of in-situ observations of magnetic reconnection in space and for detecting and analyzing the diffusion regions where ions and/or electrons are demagnetized. We focus on recent advances in the era of the Magnetospheric Multiscale mission, which has made electron-scale, multi-point measurements of magnetic reconnection in and around Earth’s magnetosphere.

The toxic effects that organic solvents have on whole cells are important drawbacks in the application of these solvents in the production of fine chemicals by whole-cell stereoselective biotransformations. Although early studies found that organic solvents mainly destroyed the integrity of cell membranes by accumulating in the lipid bilayer of plasma membranes, the cellular metabolic responses to the presence of an organic solvent remain unclear. With the rapid development of genomics, it is possible to study cellular metabolism under perturbed conditions at the genome level. In this paper, the global gene expression profiles of Saccharomyces cerevisiae BY4743 grown in media with a high concentration of the organic solvent dimethyl sulfoxide (DMSO) were determined by microarray analysis of ~6,200 yeast open reading frames (ORFs). From cells grown in SD minimal medium containing 1.0% (v/v) DMSO, changes in transcript abundance greater than or equal to 2.5-fold were classified. Genomic analyses showed that 1,338 genes were significantly regulated by the presence of DMSO in yeast. Among them, only 400 genes were previously found to be responsive to general environmental stresses, such as temperature shock, amino acid starvation, nitrogen source depletion, and progression into stationary phase. The DMSO-responsive genes were involved in a variety of cellular functions, including carbohydrate, amino acid and lipid metabolism, cellular stress responses, and energy metabolism. Most of the genes in the lipid biosynthetic pathways were down-regulated by DMSO treatment, whereas genes involved in amino acid biosynthesis were mostly up-regulated. The results demonstrate that the application of microarray technology allows better interpretation of metabolic responses, and the information obtained will be useful for the construction of engineered yeast strains with better tolerance of organic solvents.

Field-particle energy exchange is important to the magnetic reconnection process, but uncertainties regarding the time evolution of this exchange remain. We investigate the temporal dynamics of field-particle energy exchange during magnetic reconnection, using Magnetospheric Multiscale mission observations of an electron-only reconnection event in the magnetosheath. The electron energy is in local minimum at the x-line due to a density depletion, while the magnetic energy is in local maximum due to a guide field enhancement. The electromagnetic energy transport comes almost entirely from guide field contributions and is confined within the reconnection plane, while the most significant contribution to electron energy transport is independent of the drift velocity with additional out-of-plane signatures. Multi-spacecraft analysis suggests that the guide field energy is decreasing while the electron density is increasing, both evolving such that the system is moving toward a more uniform distribution of magnetic and thermal energy. The exchange of electromagnetic and thermal energy in collisionless plasmas is an important area of study to understand many space physics processes. The authors use in-situ, high resolution measurements from the MMS mission to examine the spatiotemporal evolution of the electron thermal and electromagnetic energy landscape during an encounter with a magnetic reconnection site in the Earth’s magnetosphere.

Structural and nonstructural regions of the HCV-encoded polyprotein have been expressed in recombinant yeast, bacteria, or insect cells and used to capture and measure reactive antibodies circulating in different individuals. The putative nucleocapsid protein (C) and nonstructural proteins 3-5 (NS3-NS5) were found to contain the most immunodominant epitopes. The NS3, NS4, and C regions were expressed in yeast in the form of a fused, chimeric polyprotein (C25) and a capture assay for reactive antibody was developed. This anti-C25 assay detects all previously identified HCV-seropositive cases and provides a substantially more sensitive diagnostic for both acute and chronic HCV infections than the current anti-C100-3 (NS4) assay. Anti-C25 was detected more frequently than anti-C100-3 in chronic, transfusion-associated non-A, non-B hepatitis patients from the United States (95% vs. 71%) and Japan (98% vs. 82%), in cryptogenic cirrhosis patients from the United States (62% vs. 28%), and in hepatitis B surface antigen-negative cases of hepatocellular carcinoma from Japan (83% vs. 63%). These data indicate that HCV has a greater role in these liver diseases than was previously thought. In volunteer United States blood donors sampled following the introduction of anti-C100-3 screening, the prevalence of anti-C25 and anti-C100-3 was 0.5% and 0.08%, respectively.

A specific assay has been developed for a blood-borne non-A, non-B hepatitis (NANBH) virus in which a polypeptide synthesized in recombinant yeast clones of the hepatitis C virus (HCV) is used to capture circulating viral antibodies. HCV antibodies were detected in six of seven human sera that were shown previously to transmit NANBH to chimpanzees. Assays of ten blood transfusions in the United States that resulted in chronic NANBH revealed that there was at least one positive blood donor in nine of these cases and that all ten recipients seroconverted during their illnesses. About 80 percent of chronic, post-transfusion NANBH (PT-NANBH) patients from Italy and Japan had circulating HCV antibody; a much lower frequency (15 percent) was observed in acute, resolving infections. In addition, 58 percent of NANBH patients from the United States with no identifiable source of parenteral exposure to the virus were also positive for HCV antibody. These data indicate that HCV is a major cause of NANBH throughout the world.

A nonglycosylated denatured form of human immunodeficiency virus (HIV) 1 glycoprotein gp120 (Env 2-3), which does not bind to CD4, was used with muramyl tripeptide as adjuvant to immunize HIV-seronegative healthy volunteers. In all the volunteers, three 50-μg injections of Env 2-3 induced priming of CD4+T cells specific for conserved regions of the native glycosylated gp120. Moreover, we found that several major histocompatibility complex class II (DR) alleles can function as restriction molecules for presentation of conserved epitopes of gp120 to T cells, implying that a T-cell response to these epitopes can be obtained in a large fraction of the population. The possibility to prime CD4+T cells specific for conserved epitopes of a HIV protein is particularly important in view of the lack of such cells in HIV-infected individuals and of a possible role that CD4+T cells may play in the development of protective immunity against AIDS.