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The spread of Taoism to the Western Regions marked the movement of Central Plains culture to the frontier, demonstrating its influence on local society. During the Han Dynasty, Central Plains culture had reached the Western Regions. With the deification of Laozi and his becoming the founder of Taoism, the story “Laozi converting the barbarians” (Laozi huahu 老子化胡), which claimed that Laozi journeyed to the west and taught the Hu people, provided the impetus for the spread of Taoism to the Western Regions. The Tang imperial family venerated Laozi and regarded Taoism as the state religion. Laozi belief, including the veneration of the man himself, his writings, his stories, and the precepts claimed to be related to him, was also used to assist in the Tang Dynasty’s governance of the Western Regions. Following the Tang Dynasty’s decline, the Central Plains’ influence receded from the Western Regions. However, due to the relaxed religious environment in the Western Regions, Taoism, which was representative of Central Plains culture, still survived there. Moreover, Taoism attempted to incorporate Islam in the Western Regions into its divine system by huahu, which might be related to the early history of exchange between the two religions.

This article examines the historical background of “Laozi huahu 老子化胡” (Laozi converting the barbarians) and investigates its generation mode and constituent elements. It also discusses and reflects on issues such as the origin of “huahu” and Laozi’s deification. The origin of “Laozi huahu” has little relationship to issues such as the divine system in the Han dynasty and Laozi’s deification. Its elements are rooted in Chinese secular ideological resources. The story of “heroes moving across borders” during the Spring and Autumn and Warring States periods, along with the notion of Laozi as the universal teacher for disciples of various schools, gave birth to the saying. An analysis of the historical background suggests that the origin of “huahu” had likely gone through a long evolutionary process, including an undocumented period where it was transmitted orally as a legend.

Declined numbers and weakened functions of intestinal stem cells (ISCs) impair the integrity of the intestinal epithelium during aging. However, the impact of intestinal microbiota on ISCs in this process is unclear. Here, using premature aging mice (telomerase RNA component knockout, Terc −/− ), natural aging mice, and in vitro colonoid models, we explore how heat-inactivated Bifidobacterium adolescentis ( B. adolescentis ) affects colon senescence. We find that B. adolescentis could mitigate colonic senescence-related changes by enhancing intestinal integrity and stimulating the regeneration of Lgr5 + ISCs via Wnt/β-catenin signaling. Furthermore, we uncover the involvement of Paneth-like cells (PLCs) within the colonic stem-cell-supporting niche in the B. adolescentis- induced ISC regeneration. In addition, we identify soluble polysaccharides (SPS) as potential effective components of B. adolescentis . Overall, our findings reveal the role of heat-inactivated B. adolescentis in maintaining the ISCs regeneration and intestinal barrier, and propose a microbiota target for ameliorating colon senescence. Here, Wang et al . show that heat-inactivated Bifidobacterium adolescentis mediates maintenance of host intestinal barrier through intestinal stem cells activation, which can potentially have translational implications for alleviation of colon senescence and related diseases.

Platelet (PLT) counting with impedance (PLT-I) is widely used but has low specificity. PLT counting with fluorescence (PLT-F), tested by the Sysmex XN series with high specificity, can be a complementary method to PLT-I. To identify red blood cell (RBC)- and PLT-related parameters as potential influencing factors for PLT-I and establish PLT reflex test rules with PLT-F. We prospectively tested both PLT-I and PLT-F in all 3480 samples. In a development data set of 3000 samples, differences between the reflex and nonreflex groups were compared and influencing factors for PLT-I were identified by logistic regression. The area under the receiver operating characteristic (ROC) curve and cutoff values were obtained by ROC curve analysis. Validation was conducted in the remaining 480 samples (validation data set). PLT-F showed comparable results with immunoplatelet counting. In logistic regression, increased micro-RBC absolute count (micro-RBC#), fragmented RBC absolute count (FRC#), PLT distribution width (PDW), mean PLT volume (MPV), PLT-large cell ratio (P-LCR), and immature PLT fraction absolute count (IPF#) were influencing factors for PLT-I. In ROC curve analysis, the cutoff values of micro-RBC#, FRC#, PDW, MPV, and P-LCR were 0.64 × 106/μL, 0.082 × 106/μL, 15.40 fL, 11.15 fL, and 33.95%, respectively. The areas under the ROC curve of micro-RBC# and FRC# were 0.77 and 0.79, respectively. Micro-RBC#, FRC#, PDW, MPV, P-LCR, and IPF# were factors affecting PLT-I. Among them, micro-RBC# and FRC# were the most impactful factors. From our study results, micro-RBC#, FRC#, MPV, PDW, and P-LCR can be used to establish reflex test rules for PLT counting in clinical work.

PurposeBased on previous researches of HoxC6, this study aims to investigate the expression levels of isoforms HoxC6-1 and HoxC6-2 and to explore their roles in gastric carcinogenesis as well as the possible molecular mechanism.MethodsWe investigated expression levels of HoxC6, HoxC6-1, and HoxC6-2 in gastric cancer tissues, coupled with relevant data in TCGA dataset. In vitro, HoxC6, HoxC6-1, and HoxC6-2 knockdown by small interference RNA was carried for evaluating the changes of malignant biological behaviors of gastric cancer cells, such as proliferation, migration, invasion, apoptosis, and cell cycle alternation. Metastasis-related nucleotide lncRNA HOTAIR was selected by bioinformatics, and verification was carried out by in vitro researches.ResultsData suggested HoxC6-1 and HoxC6-2 were considerably over-expressed with different folds in gastric cancerous tissues. Decreased expression of HoxC6-2 was detected in well-differentiated type of gastric cancer. In vitro, the conclusion that HoxC6 functions as a tumor oncogene illuminated by previous studies was verified again. Additionally, down-regulating of HoxC6-2 significantly inhibited SGC-7901 and BGC-823 cells from proliferation, migration, invasion, apoptosis, while quite slight results or none statistically significant results were observed when HoxC6-1 was knockdown. Besides, over-expression of HOTAIR, which is relevant with HoxC6 during gastric carcinogenesis, was detected in gastric cancerous tissues. Restored expression of HoxC6 partially reversed the decreased migration caused by down-regulating HOTAIR in gastric cancer cells.ConclusionHoxC6 acts as an oncogene in gastric carcinogenesis and might be a promising therapeutic target. Isoform HoxC6-2 plays a primary carcinogenic role in gastric carcinogenesis. HOTAIR, over-expressed in gastric cancer, might regulate HoxC6 on the protein level in promoting migration of gastric cells.

With the increasing number of aged population and growing burden of healthy aging demands, a rational standard for evaluation aging is in urgent need. The advancement of medical testing technology and the prospering of artificial intelligence make it possible to evaluate the biological status of aging from a more comprehensive view. In this review, we introduced common aging biomarkers and concluded several famous aging clocks. Aging biomarkers reflect changes in the organism at a molecular or cellular level over time while aging clocks tend to be more of a generalization of the overall state of the organism. We expect to construct a framework for aging evaluation measurement from both micro and macro perspectives. Especially, population-specific aging clocks and multi-omics aging clocks may better fit the demands to evaluate aging in a comprehensive and multidimensional manner and make a detailed classification to represent different aging rates at tissue/organ levels. This framework will promisingly provide a crucial basis for disease diagnosis and intervention assessment in geroscience.

Aging‐induced cognitive impairment is associated with a loss of metabolic homeostasis and plasticity. An emerging idea is that targeting key metabolites is sufficient to impact the function of other organisms. Therefore, more metabolism‐targeted therapeutic intervention is needed to improve cognitive impairment. We first conducted untargeted metabolomic analyses and 16S rRNA to identify the aging‐associated metabolic adaption and intestinal microbiome change. Untargeted metabolomic analyses of plasma revealed L‐arginine metabolic homeostasis was altered during the aging process. Impaired L‐arginine metabolic homeostasis was associated with low abundance of intestinal Akkermansia muciniphila ( AKK ) colonization in mice. Long‐term supplementation of AKK outer membranes protein‐Amuc_1100, rescued the L‐arginine level and restored cognitive impairment in aging mice. Mechanically, Amuc_1100 acted directly as a source of L‐arginine and enriched the L‐arginine‐producing bacteria. In aged brain, Amuc_1100 promoted the superoxide dismutase to alleviated oxidation stress, and increased nitric oxide, derivatives of L‐arginine, to improve synaptic plasticity. Meanwhile, L‐arginine repaired lipopolysaccharide‐induced intestinal barrier damage and promoted growth of colon organoid. Our findings indicated that aging‐related cognitive impairment was closely associated with the disorders of L‐arginine metabolism. AKK ‐derived Amuc_1100, as a potential postbiotic, targeting the L‐arginine metabolism, might provide a promising therapeutic strategy to maintain the intestinal homeostasis and cognitive function in aging.

Soil salinisation, exacerbated by climate change and human activities such as irrigation mismanagement, improper land use and excessive fertilisation, has become a major constraint on global crop production by disrupting fundamental metabolic processes like seed germination and photosynthesis. In our previous work, transcriptome sequencing of salt‐tolerant and salt‐sensitive cotton germplasms identified Gh_D04G136300, a negative regulatory gene downregulated in salt‐tolerant and salt‐sensitive materials. Phylogenetic analysis revealed its closest homologue to be AtBGH2, leading to its designation as GhBGH2. Virus‐induced gene silencing (VIGS) demonstrated that GhBGH2 silencing enhanced salt tolerance. To further validate its function, we generated bgh2 knockout mutants via CRISPR/Cas9, which exhibited increased salt tolerance compared to controls. Transcriptome sequencing and yeast two‐hybrid screening identified GhGLK1 as an interacting protein. Both GhBGH2 and GhGLK have nuclear localisation. Functional characterisation through VIGS revealed that GhGLK1 positively regulates salt tolerance in cotton. Yeast one‐hybrid (Y1H), dual‐luciferase (LUC) and electrophoretic mobility shift assays (EMSA) confirmed that GhGLK1 binds to G‐box elements in the promoters of downstream salt‐tolerance genes, activating their transcription. Structural analysis of GhGLK1 revealed a transcriptional activation domain at its C‐terminus, and yeast heterologous expression along with co‐immunoprecipitation (Co‐IP) assays demonstrated that GhBGH2 interacts with this domain. Haplotype analysis of GhGLK1 identified a distinct Hap‐1 variant enriched in China's northwestern saline‐alkali regions. This variant exhibited elevated GhGLK1 expression and conferred enhanced salt tolerance. Collectively, our findings indicate that GhBGH2 negatively regulates salt tolerance in cotton by interacting with the GhGLK1 activation domain, suppressing its transcriptional regulation of salt‐tolerance genes.

In this study, samples were collected from different types of artificial fish reefs and prevention and control areas in the sea areas of the northern part of Da Changshan Island and the northeastern part of Xiao Changshan Island in the North Yellow Sea. The purpose is to compare the differences in the bacterial communities among different regions, determine the impacts of environmental factors on the bacterial communities, and evaluate the ecological effects of artificial fish reefs on the marine bacterial communities. We obtained a total of 2,128,186 effective sequences and 4321 bacterial operational taxonomic units (OTUs), which were classified into 14 phyla and 76 genera. Proteobacteria were the most abundant phylum across the 32 samples, followed by Bacteroidetes. We found that all samples from the deep-sea control area exhibited the highest bacterial richness. In addition, all samples from the shallow-water concrete reef exhibited high community richness. The distribution of bacterial communities showed differences among different regions. In two specific sea areas, the bacteria in the sediment samples exhibited particularly remarkable characteristics of high diversity. Importantly, environmental factors significantly influence bacterial communities. In seawater samples, salinity (Sal) and dissolved oxygen (DO) were the primary factors affecting bacterial communities. Furthermore, grain size (GS) emerged as the most critical physicochemical factor influencing bacterial communities in sediment. This study compared the characteristics of bacterial communities in different types of artificial reefs and control areas in two marine ranches and revealed the main environmental factors affecting the bacterial communities. This is of great significance for protecting biodiversity and evaluating the ecological effects of artificial reef placement.