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The Richtmyer–Meshkov instability of heavy/light single-mode (SM), trapezoid (TR) and sawtooth (ST) interfaces is studied experimentally by considering the reshock. The TR and ST interfaces can be expanded into Fourier series with a dominant fundamental mode and more high-order modes, recognized as quasi-single-mode ones. In experiments, the distorted interfaces at the time of first reshock arrival develop in the weakly nonlinear stage, ensuring an approximate single-scale function of evolving interface. The results show an evident memory of initial interface shapes: the bubbles and spikes of ST interface after reshock mainly develop in the streamwise direction with sharp heads, while the counterparts of TR interface tend to grow in the spanwise direction. The influences of high-order modes are amplified by the reshock, resulting in significant interface shape dependence of mixing width growths. The amplitude superposition of major odd-order modes promotes the growth rates of mixing widths for the SM and ST cases, different from the TR one. The ST interface has larger mixing width growth rates in comparison with the SM interface, since high-order modes play a great role in promoting the increase of ST amplitudes, while the TR interface has a relatively small one. The linear and nonlinear mixing width growths of SM, TR and ST interfaces before and after reshock are further analysed theoretically, indicating that the fundamental mode still has a predominant influence on the interface evolution after reshock, and the growth behaviours exhibit strong similarities to those for the singly shocked cases.

The breakup of coaxial cone-jet interfaces to compound droplets in axisymmetric co-flow focusing (CFF) upon actuation is studied through numerical simulations. Due to the coupling effect of double interfaces, the response behaviours of coaxial cone-jet flow to actuation are more complex than those of a single-layered interface structure. Particularly, the coaxial jet presents totally different response modes between weak and strong interface coupling situations. In this work, the phase diagrams of response modes for coaxial jet breakup are depicted, considering the effect of perturbation frequency, amplitude and liquid flow rates. In particular, the breakup of a coaxial jet can be synchronized with actuation within a frequency range containing the natural breakup frequency, resulting in uniform compound droplets with a single core inside the shell, and the size of droplets can be adjusted by frequency. As the perturbation frequency exceeds the upper critical value, the external perturbation is unable to dominate the jet breakup, while below the lower critical frequency, the jet breaks up with multiple droplets generated in one period. The perturbation amplitude mainly affects the jet breakup length and also leads to the transition between different response modes. The coaxial cone upstream of the orifice can act as a buffer layer, regulating the perturbation amplitude of the coaxial jet downstream. The degree of buffering effect is affected by the perturbation frequency and amplitude. As the perturbation amplitude approaches unity, the decrease of perturbation frequency leads to the intermittent jet behaviour from the cone tip with a vibrating manner of the coaxial cone. Based on the linear instability analysis on the simplified single jet models for weak-coupled and strong-coupled jets, scaling analyses are carried out, which predict the jet breakup length and the natural frequency and critical frequency for the synchronized breakup. Finally, a strong pulse is added on the perturbation to produce compound droplets with a controllable number of cores. The present work provides valuable guidance for the practical application of on-demand compound droplet generation through active CFF.

The fingers known as bubbles (spikes) resulting from the penetration of light (heavy) fluids into heavy (light) fluids are significant large-scale features of Richtmyer–Meshkov instability (RMI). Through shock-tube experiments, we study finger collisions in light fluid layers under reshock conditions. Four unperturbed fluid layers with varying thicknesses are created to analyse the motion of waves and interfaces during finger collisions. The wave dynamics, sensitive to initial layer thicknesses, are characterized by a one-dimensional theory. Eight perturbed fluid layers, with four thicknesses and two interface phase combinations, are generated to explore the finger collision mechanism. It is shown that after reshock, the initial in-phase and anti-phase cases undergo spike–bubble rear-end collisions (SBCs) and spike–spike head-on collisions (SSCs), respectively. Compared with SBCs, SSCs significantly suppress spike growth, leading to the attenuation of perturbation growth, especially for larger thicknesses. As the initial thickness decreases, an SSC impedes the downstream interface from reversing its phase, resulting in abnormal RMI, thereby reducing the SSC's effectiveness in attenuating growth. The effects of rarefaction waves enhance both interfaces’ amplitudes and the whole layer's thickness, diminishing the intensity of finger collisions, while the second reshock exerts an opposing influence. Linear and nonlinear models, incorporating the influence of reshocks and rarefaction waves, are developed to predict the interface perturbation growth before and after finger collisions.

Experiments on divergent Richtmyer–Meshkov (RM) instability at a heavy gas layer are performed in a specially designed shock tube. A novel soap-film technique is extended to generate gas layers with controllable thicknesses and shapes. An unperturbed gas layer is first examined and its two interfaces are found to move uniformly at the early stage and be decelerated later. A general one-dimensional theory applicable to an arbitrary-thickness layer is established, which gives a good prediction of the layer motion in divergent geometry. Then, six kinds of perturbed SF$_6$ layers with various thicknesses and shapes surrounded by air are examined. At the early stage, the amplitude growths of the inner interface for various-thickness layers collapse quite well and also can be predicted by the Bell model for cylindrical RM instability at a single interface, which indicates a negligible interface coupling effect. Later, a rarefaction wave accelerates the inner interface, causing a dramatic rise in the growth rate. It is found that a thicker gas layer will result in a larger extent that the rarefaction wave can promote the instability growth. A modified Bell model accounting for both Rayleigh–Taylor (RT) instability and interface stretching caused by a rarefaction wave is established, which well reproduces the quick instability growth. At late stages, reverberating waves inside the layer are negligibly weak such that the inner interface growth is dominated by RM instability and RT stability. The major factors driving the outer interface development are a compression wave and interface coupling. A new interface coupling phenomenon existing uniquely in divergent geometry caused by the gradual thinning of the gas layer is observed and also modelled.

In photosynthetic organisms, the photosystem II (PSII) complex is the primary target of thermal damage. Plants have evolved a repair process to prevent the accumulation of damaged PSII. The repair of PSII largely involves de novo synthesis of proteins, particularly the D1 subunit protein encoded by the chloroplast gene psbA . Here we report that the allotropic expression of the psbA complementary DNA driven by a heat-responsive promoter in the nuclear genome sufficiently protects PSII from severe loss of D1 protein and dramatically enhances survival rates of the transgenic plants of Arabidopsis , tobacco and rice under heat stress. Unexpectedly, we found that the nuclear origin supplementation of the D1 protein significantly stimulates transgenic plant growth by enhancing net CO 2 assimilation rates with increases in biomass and grain yield. These findings represent a breakthrough in bioengineering plants to achieve efficient photosynthesis and increase crop productivity under normal and heat-stress conditions. Heat stress damages photosystems, especially photosystem II (PSII), thus affecting photosynthetic efficiency. To counteract the thermal damage, a new bioengineering strategy is introduced by expressing a PSII subunit D1 under the control of a heat-responsive promoter in the nuclear genome. The strategy has been tested and found to be effective in Arabidopsis , tobacco and rice.

Instability and rupture dynamics of a liquid nano-thread, subjected to external hydrodynamic perturbations, are captured by a stochastic lubrication equation (SLE) incorporating thermal fluctuations via Gaussian white noise. Linear instability analysis of the SLE is conducted to derive the spectra and distribution functions of thermal capillary waves influenced by external perturbations and thermal fluctuations. The SLE is also solved numerically using a second-order finite difference method with a correlated noise model. Both theoretical and numerical solutions, validated through molecular dynamics, indicate that surface tension forces due to specific external perturbations overcome the random effects of thermal fluctuations, determining both the thermal capillary waves and the evolution of perturbation growth. The results also show two distinct regimes: (i) the hydrodynamic regime, where external perturbations dominate, leading to uniform ruptures, and (ii) the thermal-fluctuation regime, where external perturbations are surpassed by thermal fluctuations, resulting in non-uniform ruptures. The transition between these regimes, modelled by a criterion developed from linear instability theory, exhibits a strong dependence on the amplitudes and wavenumbers of the external perturbations.

Boundary conditions at a liquid–solid interface are crucial to dynamics of a liquid film coated on a fibre. Here, a theoretical framework based on axisymmetric Stokes equations is developed to explore the influence of liquid–solid slip on the Rayleigh–Plateau instability of a cylindrical film on a fibre. The new model not only shows that the slip-enhanced growth rate of perturbations is overestimated by the classical lubrication model, but also indicates a slip-dependent dominant wavelength, instead of a constant value obtained by the lubrication method, which leads to larger drops formed on a more slippery fibre. The theoretical findings are validated by direct numerical simulations of Navier–Stokes equations via a volume-of-fluid method. Additionally, the slip-dependent dominant wavelengths predicted by our model agree with the experimental results provided by Haefner et al. (Nat. Commun., vol. 6, issue 1, 2015, 7409).

The Richtmyer–Meshkov instability of a helium layer surrounded by air is studied in a semi-annular convergent shock tube by high-speed schlieren photography. The gas layer is generated by an improved soap film technique such that its boundary shapes and thickness are precisely controlled. It is observed that the inner interface of the shocked light gas layer remains nearly undisturbed during the experimental time, even after the reshock, which is distinct from its counterpart in the heavy gas layer. This can be ascribed to the faster decay of the perturbation amplitude of the transmitted shock in the helium layer and Rayleigh–Taylor stabilization on the inner surface (light/heavy) during flow deceleration. The outer interface first experiences ‘accelerated’ phase inversion owing to geometric convergence, and later suffers a continuous deformation. Compared with a sole heavy/light interface, the wave influence (interface coupling) inhibits (promotes) growth of instability of the outer interface.

Early-life stress (ELS) is thought to cause the development of visceral pain disorders. While some individuals are vulnerable to visceral pain, others are resilient, but the intrinsic circuit and molecular mechanisms involved remain largely unclear. Herein, we demonstrate that inbred mice subjected to maternal separation (MS) could be separated into susceptible and resilient subpopulations by visceral hypersensitivity evaluation. Through a combination of chemogenetics, optogenetics, fiber photometry, molecular and electrophysiological approaches, we discovered that susceptible mice presented activation of glutamatergic projections or inhibition of GABAergic projections from the anteroventral bed nucleus of the stria terminalis (avBNST) to paraventricular nucleus (PVN) corticotropin-releasing hormone (CRH) neurons. However, resilience develops as a behavioral adaptation partially due to restoration of PVN SK2 channel expression and function. Our findings suggest that PVN CRH neurons are dually regulated by functionally opposing avBNST neurons and that this circuit may be the basis for neurobiological vulnerability to visceral pain.

We report the first shock-tube experiments on Richtmyer–Meshkov instability at a single-mode light–heavy interface accelerated by a strong shock wave with Mach number higher than 3.0. Under the proximity effect of the transmitted shock and its induced secondary compression effect, the interface profile is markedly different from that in weakly compressible flows. For the first time, the validity of the compressible linear theory and the failure of the impulsive model in predicting the linear amplitude evolution in highly compressible flows are verified through experiments. Existing nonlinear and modal models fail to accurately describe the perturbation evolution, as they do not account for the shock proximity and secondary compression effects on interface evolution. The shock proximity effect manifests mainly in the early stages when the transmitted shock remains close to the interface, while the effect of secondary compression manifests primarily at the period when interactions of transverse shocks occur at the bubble tips. Based on these findings, we propose an empirical model capable of predicting the bubble evolution in highly compressible flows.