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"Singh, K."
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Mapping QTLs for Salt Tolerance in Rice (Oryza sativa L.) by Bulked Segregant Analysis of Recombinant Inbred Lines Using 50K SNP Chip
Soil salinity is a major constraint to rice production in large inland and coastal areas around the world. Modern high yielding rice varieties are particularly sensitive to high salt stress. There are salt tolerant landraces and traditional varieties of rice but with limited information on genomic regions (QTLs) and genes responsible for their tolerance. Here we describe a method for rapid identification of QTLs for reproductive stage salt tolerance in rice using bulked segregant analysis (BSA) of bi-parental recombinant inbred lines (RIL). The number of RILs required for the creation of two bulks with extreme phenotypes was optimized to be thirty each. The parents and bulks were genotyped using a 50K SNP chip to identify genomic regions showing homogeneity for contrasting alleles of polymorphic SNPs in the two bulks. The method was applied to 'CSR11/MI48' RILs segregating for reproductive stage salt tolerance. Genotyping of the parents and RIL bulks, made on the basis of salt sensitivity index for grain yield, revealed 6,068 polymorphic SNPs and 21 QTL regions showing homogeneity of contrasting alleles in the two bulks. The method was validated further with 'CSR27/MI48' RILs used earlier for mapping salt tolerance QTLs using low-density SSR markers. BSA with 50K SNP chip revealed 5,021 polymorphic loci and 34 QTL regions. This not only confirmed the location of previously mapped QTLs but also identified several new QTLs, and provided a rapid way to scan the whole genome for mapping QTLs for complex agronomic traits in rice.
Journal Article
SAP Sales Cloud : sales force automation with SAP C/4HANA
\"Looking for the tools to boost your sales sky high? With this comprehensive guide, you'll learn to implement, configure, and use SAP Sales Cloud. Create leads, process opportunities, and explore partner channel management. Then integrate the solution with your ERP system to handle quotations and orders. Finally, migrate and replicate your existing sales data and personalize and extend SAP Sales Cloud\"-- Provided by publisher.
Book
Development and evaluation of near-isogenic lines for major blast resistance gene(s) in Basmati rice
KEY MESSAGE : A set of NILs carrying major blast resistance genes in a Basmati rice variety has been developed. Also, the efficacy of pyramids over monogenic NILs against rice blast pathogen Magnaporthe oryzae has been demonstrated. Productivity and quality of Basmati rice is severely affected by rice blast disease. Major genes and QTLs conferring resistance to blast have been reported only in non-Basmati rice germplasm. Here, we report incorporation of seven blast resistance genes from the donor lines DHMASQ164-2a (Pi54, Pi1, Pita), IRBLz5-CA (Pi2), IRBLb-B (Pib), IRBL5-M (Pi5) and IRBL9-W (Pi9) into the genetic background of an elite Basmati rice variety Pusa Basmati 1 (PB1). A total of 36 near-isogenic lines (NILs) comprising of 14 monogenic, 16 two-gene pyramids and six three-gene pyramids were developed through marker-assisted backcross breeding (MABB). Foreground, recombinant and background selection was used to identify the plants with target gene(s), minimize the linkage drag and increase the recurrent parent genome (RPG) recovery (93.5–98.6 %), respectively, in the NILs. Comparative analysis performed using 50,051 SNPs and 500 SSR markers revealed that the SNPs provided better insight into the RPG recovery. Most of the monogenic NILs showed comparable performance in yield and quality, concomitantly, Pusa1637-18-7-6-20 (Pi9), was significantly superior in yield and stable across four different environments as compared to recurrent parent (RP) PB1. Further, among the pyramids, Pusa1930-12-6 (Pi2+Pi5) showed significantly higher yield and Pusa1633-7-8-53-6-8 (Pi54+Pi1+Pita) was superior in cooking quality as compared to RP PB1. The NILs carrying gene Pi9 were found to be the most effective against the concoction of virulent races predominant in the hotspot locations for blast disease. Conversely, when analyzed under artificial inoculation, three-gene pyramids expressed enhanced resistance as compared to the two-gene and monogenic NILs.
Journal Article
Fundamentals of capturing and processing drone imagery and data
\"Unmanned Aircraft Systems are rapidly emerging as flexible platforms for capturing imagery and other data across sciences. Many colleges and universities are developing courses on UAS-based data acquisition. This book is a comprehensive, introductory text on how to use unmanned aircraft systems for data capture and analysis. It provides best practices for planning data capture missions and hands-on learning modules geared toward UAS data collection, processing, and applications. Readers will learn how to process different types of UAS imagery for applications such as Precision Agriculture, Forestry, Urban Landscapes, and apply this knowledge in environmental monitoring and land-use studies\"-- Provided by publisher.
Book
Celestial operator product expansions and w1+∞ symmetry for all spins
A
bstract
The operator product expansion of massless celestial primary operators of arbitrary spin is investigated. Poincaré symmetry is found to imply a set of recursion relations on the operator product expansion coefficients of the leading singular terms at tree-level in a holomorphic limit. The symmetry constraints are solved by an Euler beta function with arguments that depend simply on the right-moving conformal weights of the operators in the product. These symmetry-derived coefficients are shown not only to match precisely those arising from momentum-space tree-level collinear limits, but also to obey an infinite number of additional symmetry transformations that respect the algebra of w
1+
∞
. In tree-level minimally-coupled gravitational theories, celestial currents are constructed from light transforms of conformally soft gravitons and found to generate the action of w
1+∞
on arbitrary massless celestial primaries. Results include operator product expansion coefficients for fermions as well as those arising from higher-derivative non-minimal couplings of gluons and gravitons.
Journal Article
Migration, gender and home economics in rural North India
\"This book critically examines the socio-economic impacts of out-migration on households and gender dynamics in rural northern India. The first of its kind, this study unearths, through detailed regional and demographical research, the ways in which economic and migratory trends of male family members in rural India in general, and hilly regions of Garhwal in particular, affect the wives, children, extended families, and agricultural lands that they have left behind. It offers vital research in how rural India's socio-economic formations and topographic characteristics can today more effectively contribute to the national and global economy with respect to migratory trends, gender dynamics, and home life. Furthermore, it investigates the collapse of agricultural and many other traditional economic activities without a corresponding creation of fresh economic opportunities. This volume moreover elucidates how male out-migration from rural to urban centres has greatly re-shaped kinship and economic structures at places of origin and consequently had a serious impact on the socio-psychological well- being of family members This volume will be of great value to scholars and researchers of development economics, agricultural economics, environment studies, sociology, social anthropology, population studies, gender and women's studies, social psychology, migration and diaspora studies, South Asian studies, and behavioral studies\"-- Provided by publisher.
Book
Nucleotide metabolism: a pan-cancer metabolic dependency
Metabolic alterations are a key hallmark of cancer cells, and the augmented synthesis and use of nucleotide triphosphates is a critical and universal metabolic dependency of cancer cells across different cancer types and genetic backgrounds. Many of the aggressive behaviours of cancer cells, including uncontrolled proliferation, chemotherapy resistance, immune evasion and metastasis, rely heavily on augmented nucleotide metabolism. Furthermore, most of the known oncogenic drivers upregulate nucleotide biosynthetic capacity, suggesting that this phenotype is a prerequisite for cancer initiation and progression. Despite the wealth of data demonstrating the efficacy of nucleotide synthesis inhibitors in preclinical cancer models and the well-established clinical use of these drugs in certain cancer settings, the full potential of these agents remains unrealized. In this Review, we discuss recent studies that have generated mechanistic insights into the diverse biological roles of hyperactive cancer cell nucleotide metabolism. We explore opportunities for combination therapies that are highlighted by these recent advances and detail key questions that remain to be answered, with the goal of informing urgently warranted future studies.Overactive nucleotide synthesis is a hallmark of cancers and inhibitors of nucleotide synthesis pathways have shown promise in some cancer types. In this Review, Mullen and Singh give an overview of the role of aberrant nucleotide synthesis in supporting cancer cell growth, immune evasion, metastasis and resistance to cancer therapies, with a focus on identifying opportunities for the use of combination therapies to target these pathways more effectively.
Journal Article
Financial market regulations and legal challenges in South Asia
\"This book addresses the difficulties and challenges of the regulatory environment in South Asia, outlining the apparent issues and resolutions as these developing nations transition into global economic players\"-- Provided by publisher.
Book
Detection and characterization of fungus (Magnaporthe oryzae pathotype Triticum) causing wheat blast disease on rain-fed grown wheat (Triticum aestivum L.) in Zambia
Wheat blast caused by Magnaporthe oryzae pathotype Triticum (MoT) is a threat to wheat production especially in the warmer-humid environments. In Zambia, wheat blast symptoms were observed for the first time on wheat (Triticum aestivum L.) grown in experimental plots and five farmers' fields in Mpika district of Muchinga Province during the 2017-18 rainy season. Infected plants showed the typical wheat blast symptoms with the spike becoming partially or completely bleached with the blackening of the rachis in a short span of time. Incidence of blast symptoms on nearly all wheat heads was high and ranged from 50 to 100%. Examination of diseased plant leaves showed the presence of elliptical, grayish to tan necrotic lesions with dark borders on the leaf often mixed with other foliar diseases. A study was conducted to isolate and identify the causal pathogen(s) using classical and molecular methods and determine the pathogenicity of the detected disease causal agent. Morphobiometrical determination of causal pathogen revealed conidia with characteristic pear shaped 2-septate hyaline spores associated with M. oryzae species. Preliminary polymerase chain reaction screening of six isolates obtained from wheat blast infected samples with diagnostic primers (MoT3F/R) was conducted at ZARI, Zambia, and subsequent analysis of two isolates with MoT3F/R and C17F/R was performed at USDA-ARS, USA. Both experiments confirmed that MoT is the causal agent of wheat blast in Zambia. Further, pathogenicity tests performed with pure culture isolates from samples WS4 and WS5 produced typical blast symptoms on all the six inoculated wheat genotypes. Results of this study indicate that MoT is causing wheat blast in rain-fed wheat grown in Zambia, thus making it the first report of MoT in Zambia and Africa. This inter-continental movement of the pathogen (disease) has serious implication for wheat production and trade that needs to be urgently addressed.
Journal Article