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Aptamers, commonly referred to as chemical antibodies, are used in a wide range of applications including drug delivery and biosensing. However, the process of aptamer selection poses a substantial challenge, as it requires numerous cycles of enrichment and involves issues with nonspecific binding. We present a simple, fast instrument-free method for aptamer enrichment and selection based on a diffusion-binding process in a three-dimensional non-fouling porous hydrogel with immobilized target proteins. Low-affinity aptamer candidates can be rapidly released from the hydrogel, whereas high-affinity candidates are restricted due to their strong binding to the immobilized protein targets. Consequently, a one-step enriched aptamer pool can strongly bind the protein targets. This enrichment is consistent across five proteins with isoelectric points in varying ranges. With thrombin as a representative model, the anti-thrombin aptamer identified from an enriched aptamer pool has been found to have a binding affinity that is comparable to those identified over ten cycles of selection using traditional methods. A non-fouling, porous hydrogel makes aptamer selection and enrichment simple and efficient.

Objective: Continuous thoracic epidural analgesia (TEA) is compared with erector spinae plane (ESP) block for the perioperative pain management in patients undergoing cardiac surgery for the quality of analgesia, incentive spirometry, ventilator duration, and intensive care unit (ICU) duration. Methodology: A prospective, randomized comparative clinical study was conducted. A total of 50 patients were enrolled, who were randomized to either Group A: TEA (n = 25) or Group B: ESP block (n = 25). Visual analog scale (VAS) was recorded in both the groups during rest and cough at the various time intervals postextubation. Both the groups were also compared for incentive spirometry, ventilator, and ICU duration. Statistical analysis was performed using the independent Student's t-test. A value of P < 0.05 was considered statistically significant. Results: C omparable VAS scores were revealed at 0 h, 3 h, 6 h, and 12 h (P > 0.05) at rest and during cough in both the groups. Group A had a statistically significant VAS score than Group B (P ≤ 0.05) at 24 h, 36 h, and 48 h but mean VAS in either of the Group was ≤4 both at rest and during cough. Incentive spirometry, ventilator, and ICU duration were comparable between the groups. Conclusion: ESP block is easy to perform and can serve as a promising alternative to TEA in optimal perioperative pain management in cardiac surgery.

The inconsistent Hubble constant values derived from cosmic microwave background (CMB) observations and from local distance-ladder measurements may suggest new physics beyond the standard ACDM paradigm. It has been found in earlier studies that, at least phenomenologically, non-standard recombination histories can reduce the ≳ 4δ Hubble tension to ∼ 2δ. Following this path, we vary physical and phenomenological parameters in RECFAST, the standard code to compute ionization history of the universe, to explore possible physics beyond standard recombination. We find that the CMB constraint on the Hubble constant is sensitive to the hydrogen ionization energy and 2 s → 1 s two-photon decay rate, both of which are atomic constants, and is insensitive to other details of recombination. Thus, the Hubble tension is very robust against perturbations of recombination history, unless exotic physics modifies the atomic constants during the recombination epoch.

Peanut (Arachis hypogaea L) is one of the widely cultivated and leading oilseed crops of the world and its yields are greatly affected by various biotic and abiotic stresses. Arachis diogoi, a wild relative of peanut, is an important source of genes for resistance against various stresses that affect peanut. In our previous study a thaumatin-like protein gene was found to be upregulated in a differential expression reverse transcription PCR (DDRT-PCR) study using the conidial spray of the late leaf spot pathogen, Phaeoisariopsis personata. In the present study, the corresponding full length cDNA was cloned using RACE-PCR and has been designated as AdTLP. It carried an open reading frame of 726 bp potentially capable of encoding a polypeptide of 241 amino acids with 16 conserved cysteine residues. The semi-quantitative RT-PCR analysis showed that the transcript level of AdTLP increased upon treatment with the late leaf spot pathogen of peanut, P. personata and various hormone treatments indicating its involvement in both, biotic and abiotic stresses. The antifungal activity of the purified recombinant protein was checked against different fungal pathogens, which showed enhanced anti-fungal activity compared to many other reported TLP proteins. The recombinant AdTLP-GFP fusion protein was found to be predominantly localized to extracellular spaces. Transgenic tobacco plants ectopically expressing AdTLP showed enhanced resistance to fungal pathogen, Rhizoctonia solani. The seedling assays showed enhanced tolerance of AdTLP transgenic plants against salt and oxidative stress. The transcript analysis of various defense related genes highlighted constitutively higher level expression of PR1a, PI-I and PI-II genes in transgenic plants. These results suggest that the AdTLP is a good candidate gene for enhancing stress resistance in crop plants.

Climate change adds another dimension of challenges to the growth and sustainability of Indian agriculture. The growing exposure to livelihood shocks from climate variability/change and limited resource base of the rural community to adapt has reinforced the need to mainstream climate adaptation planning into developmental landscape. However, a better understanding of micro-level perceptions is imperative for effective and informed planning at the macro-level. In this paper, the grass-root level perspectives on climate change impacts and adaptation decisions were elicited at farm level in the Moga district of Punjab and Mahbubnagar district of Telangana, India. The farmers opined that the climatic variability impacts more than the long-term climate change. They observed change in the quantum, onset and distribution of rainfall, rise in minimum as well as maximum temperature levels, decline in crop yield and ground water depletion. The key socio-economic effects of climate change included decline in farm income, farm unemployment, rural migration and increased indebtedness among farmers. In order to cope with climate variability and change thereon, farmers resorted to adaptation strategies such as use of crop varieties of suitable duration, water conservation techniques, crop insurance and participation in non-farm activities and employment guarantee schemes. Farmers’ adaptation to changing climate was constrained by several technological, socio-economic and institutional barriers. These include limited knowledge on the costs–benefits of adaptation, lack of access to and knowledge of adaptation technologies, lack of financial resources and limited information on weather. Besides, lack of access to input markets, inadequate farm labour and smaller farm size were the other constraints. Further, on the basis of the grass-root elicitation a ‘Need-Based Adaptation’ planning incorporating farmers’ perceptions on climate change impacts, constraints in the adoption of adaptation strategies and plausible adaptation options were linked with the most suitable ongoing programmatic interventions of the Government of India. The study concluded that micro-level needs and constraints for various adaptation strategies and interventions should be an integral part of the programme development, implementation and evaluation in the entire developmental paradigm.

Background: Good postoperative analgesia in cardiac surgical patients helps in early recovery and ambulation. An alternative to parenteral, paravertebral, and thoracic epidural analgesia can be pectoralis nerve (Pecs) block, which is novel, less invasive regional analgesic technique. Aims: We hypothesized that Pecs block would provide superior postoperative analgesia for patients undergoing cardiac surgery through midline sternotomy compared to parenteral analgesia. Materials and Methods: Forty adult patients between the age groups of 25 and 65 years undergoing coronary artery bypass grafting or valve surgeries through midline sternotomy under general anesthesia were enrolled in the study. Patients were randomly allocated into two groups with 20 in each group. Group 1 patients did not receive Pecs block, whereas Group 2 patients received bilateral Pecs block postoperatively. Patients were extubated once they fulfilled extubation criteria. Ventilator duration was recorded. Patients were interrogated for pain by visual analog scale (VAS) scoring at rest and cough. Inspiratory flow rate was assessed using incentive spirometry. Results: Pecs group patients required lesser duration of ventilator support (P < 0.0001) in comparison to control group. Pain scores at rest and cough were significantly low in Pecs group at 0, 3, 6, 12, and 18 h from extubation (P < 0.05). At 24 h, VAS scores were comparable between two groups. Peak inspiratory flow rates were higher in Pecs group as compared to control group at 0, 3, 6, 12, 18, and 24 h (P < 0.05). Thirty-four episodes of rescue analgesia were given in control group, whereas in Pecs group, there were only four episodes of rescue analgesia. Conclusion: Pecs block is technically simple and effective technique and can be used as part of multimodal analgesia in postoperative cardiac surgical patients for better patient comfort and outcome.

Globally, agriculture is recognized as a highly vulnerable sector to climate change and risks from climatic aberrations pose an imminent danger to the food security and sustainability of livelihoods. To bring robustness in climate adaptation planning, evaluation of resilience across homogenous regions is essential for developing and scaling suitable location-need-context specific interventions and policies that build the resilience of the agricultural system. In this paper, we present an analysis and discussion of multi-scalar and multi-indicator assessment, by profiling resilience across agro-climatic zones of India, based on the development of a Climate-Resilient Agriculture Index embracing environmental, technological, socio-economic, and institutional and infrastructural dimension. A total of 26 indicators, spread across these four dimensions, were employed to purport inter- and intra-agro-climatic zone differentials in the level of resilience. Among the zones, it was found that West Coast Plains & Ghats and Tans-Gangetic Plains had the highest degree of resilience to manage climate risks. Most of the districts lying within Eastern Himalayan Region, Middle Gangetic Plains, Eastern Plateau & Hills, and Western Dry Region had a lower degree of resilience. The study places greater emphasis on deciphering region-specific drivers and barriers to resilience at a further disaggregated scale for improving rural well-beings. It is construed that devising action plans emphasizing awareness, preservation of natural resources, diversification, building physical infrastructure, strengthening of grass-root institutions, and mainstreaming climate adaptation in the developmental policy is crucial for climate-resilient pathways.

Mustard is a commercial oilseed crop worldwide infected by a highly infectious turnip mosaic virus (TuMV). In the experimental field at ICAR-IARI, New Delhi, in 2022, a 100% incidence of TuMV infection was observed in brown, black and yellow mustard. A very low aphid population suggested the possibility of seed transmission. Earlier, the virus genome was characterized by high throughput sequencing and it was a recombinant of World-B and Asian-BR isolates. The presence of TuMV in immature seeds was confirmed in eight field-grown genotypes via RT-PCR using CP-specific primers designed from the same genome sequence. TuMV was found to be localized in embryo and cotyledon, indicating its true seed-borne nature. Presence of TuMV was also confirmed by RT-PCR in the grow out plants from seeds of field grown eight infected genotypes and 9 genotypes collected from seed stock, that were grown in an aphid-free growth chamber. Further, out of 24 seedlings of Pusa Gold (seed stock) and Pusa Karishma (seeds from field grown plants), 20 and 17 seedlings were found infected with TuMV, respectively. The internally seed-borne nature of the virus leads to its early establishment at the seedling stage, leading to stunting and leaf-puckering symptoms in the progeny plants. This study is the first evidence of seed embryo infection and seedling transmission of TuMV of all the three species of mustard plants (brown, black and yellow mustard). Seed transmission of TuMV in mustard genotypes have implications for the seed exchange programme of mustard seeds.

We investigate the scalar perturbation produced at the pre-inflationary stage driven by a massive scalar field in Eddington-inspired Born–Infeld gravity. The scalar power spectrum exhibits a peculiar rise for low k -modes. The tensor-to-scalar ratio can be significantly lowered compared with that in the standard chaotic inflation model in general relativity. This result is very affirmative considering the recent dispute on the detection of gravitational wave radiation between PLANCK and BICEP2.

The parasites of the genus Leishmania survive and proliferate in the host phagocytic cells by taking control over their microbicidal functions. The parasite also promotes differentiation of antigen-specific anti-inflammatory cytokines producing effector T cells, which eventually results in disease pathogenesis. The mechanisms that parasites employ to dominate host adaptive immunity are largely unknown. For the first time, we report that L. donovani , which causes visceral leishmaniasis in the Indian subcontinent, upregulates the expression of an immune inhibitory receptor i.e., CD300a on antigen presenting and phagocytic cells to dampen their effector functions. The blocking of CD300a signals in leishmania antigens activated macrophages and dendritic cells enhanced the production of nitric oxide, pro-inflammatory cytokines along with MHCI/II genes expression, and reduced parasitic uptake. Further, the abrogation of CD300a signals in Leishmania infected mice benefited antigen-experienced, i.e., CD4 + CD44 + and CD8 + CD44 + T cells to acquire more pro-inflammatory cytokines producing phenotypes and helped in the early clearance of parasites from their visceral organs. The CD300a receptor blocking also enhanced the conversion of CD4 + T effectors cells to their memory phenotypes i.e., CCR7 high CD62L high up to 1.6 and 1.9 fold after 14 and 21 days post-infection, respectively. These findings implicate that CD300a is an important determinant of host phagocytic cells functions and T cells differentiation against Leishmania antigens.