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The Advanced Proton Driven Plasma Wakefield Acceleration Experiment (AWAKE) is a project at CERN aiming to accelerate an electron bunch in a plasma wakefield driven by a proton bunch. The plasma is induced in a 10 m long rubidium vapor cell using a pulsed Ti:Sapphire laser, with the wakefield formed by a proton bunch from the CERN Super Proton Synchrotron (SPS). A 16 MeV electron bunch is simultaneously injected into the plasma cell to be accelerated by the wakefield to energies in the GeV range over this short distance. After successful runs with the proton and laser beams, the electron beam line was installed and commissioned at the end of 2017 to produce and inject a suitable electron bunch into the plasma cell. To achieve the goals of the experiment, it is important to have reliable beam instrumentation measuring the various parameters of the proton, electron and laser beams. This contribution presents the status of the beam instrumentation in AWAKE and reports on the performance achieved during the AWAKE runs in 2017.

Innate immune responses have recently been appreciated to play an important role in the pathogenesis of HIV infection. Whereas inadequate innate immune sensing of HIV during acute infection may contribute to failure to control and eradicate infection, persistent inflammatory responses later during infection contribute in driving chronic immune activation and development of immunodeficiency. However, knowledge on specific HIV PAMPs and cellular PRRs responsible for inducing innate immune responses remains sparse. Here we demonstrate a major role for RIG-I and the adaptor protein MAVS in induction of innate immune responses to HIV genomic RNA. We found that secondary structured HIV-derived RNAs induced a response similar to genomic RNA. In primary human peripheral blood mononuclear cells and primary human macrophages, HIV RNA induced expression of IFN-stimulated genes, whereas only low levels of type I IFN and tumor necrosis factor α were produced. Furthermore, secondary structured HIV-derived RNA activated pathways to NF-κB, MAP kinases, and IRF3 and co-localized with peroxisomes, suggesting a role for this organelle in RIG-I-mediated innate immune sensing of HIV RNA. These results establish RIG-I as an innate immune sensor of cytosolic HIV genomic RNA with secondary structure, thereby expanding current knowledge on HIV molecules capable of stimulating the innate immune system.

Myocardial perfusion imaging (MPI) procedures from single photon emission computed tomography and positron emission tomography imaging have been registered in the Western Denmark Heart Registry (WDHR) since 2017 as a sub-registry (WDHR-MPI). The aim was to review the content, quality, and research potential of the WDHR-MPI and assess the completeness and validity of its key variables. Using the Danish National Patient Registry (DNPR) as a reference, we calculated the completeness of procedure registration as the proportion of procedures registered in the WDHR-MPI from 2017 to 2021. We defined completeness of variable registration as the proportion of non-missing values for a given variable and examined it for 38 key variables. We assessed validity by estimating the positive predictive values (PPV) and negative predictive values (NPV) with 95% confidence intervals (CI) for 26 selected key variables using corresponding extracted information from 300 randomly selected patient records. We identified 19,084 procedures in 17,871 patients from all seven cardiac centers performing nuclear cardiology procedures in the WDHR uptake area. The registry accumulated 4000-6000 procedures each year from 2019 to 2021. The overall completeness of procedure registration was 72% (95% CI 72-73), increasing from 14% (95% CI 13-15) in 2017 to 98% (95% CI 98-98) in 2021. The mean completeness of variable registration was 89%. The validity of entered data was high, with an overall PPV for the selected key variables of 95% (95% CI 94-95) and NPV of 94% (95% CI 93-96). The WDHR-MPI is one of the largest systematic MPI cohorts. The overall high completeness and validity of the data support its potential for cardio-epidemiological research.