Explore the vast range of titles available.

To evaluate the relationship between muscle fiber characteristics and the quality of frozen/thawed pork meat, four different muscles, M. longissimus thoracis et lumborum (LTL), M. psoas major (PM), M. semimembranosus (SM), and M. semitendinosus (ST), were analyzed from twenty carcasses. Meat color values (lightness, redness, yellowness, chroma, and hue) changed due to freezing/thawing in LTL, which showed larger IIAX, IIX, and IIXB fibers than found in SM ( P  < 0.05). SM and ST showed a significant decrease in purge loss and an increase in shear force caused by freezing/thawing ( P  < 0.05). Compared with LTL, SM contains more type IIXB muscle fibers and ST had larger muscle fibers I and IIA ( P  < 0.05). PM was the most stable of all muscles, since only its yellowness and chroma were affected by freezing/thawing ( P  < 0.05). These results suggest that pork muscle fiber characteristics of individual cuts must be considered to avoid quality deterioration during frozen storage.

The dynamic behavior of a pile-supported structure with a base isolator was investigated by using 1 g shaking table model tests considering soil–structure interaction (SSI). The emphasis was placed on evaluating the effect of the with/without developed base isolator on the dynamic behavior of end-bearing piles and structures. The experiment was performed through sweep tests and sinusoidal wave tests. As a result of the tests, the developed base isolator was found to effectively reduce the structure’s resonant frequencies and damped the response acceleration under resonance frequencies. According to sweep tests, the base shear force of the pile-supported structure system tends to decrease as the relative density of the soil increases during resonance. It showed that the base isolator tends to reduce significantly the response acceleration of not only the rigid-based structure but also the pile-supported structure. It was shown that although the isolated superstructure recorded large horizontal displacements, piles experienced reduced horizontal displacement and bending moments, regardless of soil conditions.

The microrchidia (MORC)-family CW-type zinc finger 2 (MORC2) gene is related to DNA repair, adipogenesis and epigenetic silencing via the human silencing hub (HUSH) complex. MORC2 missense mutation is known to cause peripheral neuropathy of Charcot-Marie-Tooth disease type 2 Z (CMT2Z). However, there have been reports of peripheral and central neuropathy in patients, and the disease has been co-categorized with developmental delay, impaired growth, dysmorphic facies and axonal neuropathy (DIGFAN). The etiology of MORC2 mutation-mediated neuropathy remains uncertain. Here, we established and analyzed Morc2a p.S87L mutant mice. Morc2a p.S87L mice displayed the clinical symptoms expected in human CMT2Z patients, such as axonal neuropathy and skeletal muscle weakness. Notably, we observed severe central neuropathy with cerebella ataxia, cognition disorder and motor neuron degeneration in the spinal cord, and this seemed to be evidence of DIGFAN. Morc2a p.S87L mice exhibited an accumulation of DNA damage in neuronal cells, followed by p53/cytochrome c/caspase 9/caspase 3-mediated apoptosis. This study presents a new mouse model of CMT2Z and DIGFAN with a Morc2a p.S87L mutation. We suggest that neuronal apoptosis is a possible target for therapeutic approach in MORC2 missense mutation. This article has an associated First Person interview with the first author of the paper.

In this study, the relationship between muscle fiber characteristics and meat quality of pork loin chops prepared using different directions of cut (vertical to the muscle length, M-Vertical; vertical or parallel to the muscle fiber orientation, F-Vertical or F-Parallel) was evaluated under different storage conditions (fresh, cold storage/aged, and freeze–thawed). Among the three groups, F-parallel displayed considerably larger size of muscle fibers, regardless of their type. This group also displayed an increase in discoloration in aged chops and a decrease in purge loss and tenderness than in other cut groups (p < 0.05). Freeze–thawing accelerated deterioration of meat quality, especially water-holding capacity and tenderness in all groups (p < 0.05), but was most prominent in F-Parallel. Therefore, to avoid excessive deterioration of fresh, aged, or frozen/thawed pork loin chops, it is important to consider the direction in which the chop is cut with respect to the muscle fiber orientation.

Biogenic amines (BAs), which are mainly generated by the microbial decarboxylation of amino acids, are important nitrogen compounds in fermented foods because of their toxicology. However, amino acids, the precursors of BAs, also play an important role in generating volatile and non-volatile metabolites, which are strongly associated with quality indicators for foods. Bacillus subtilis is one of dominant fermentative microorganism in various fermented foods and is well known as a BA-producing bacterium. In this study, B. subtilis strains which have different BAs-producing capacities, higher level of BAs production strain (BH) and lower level of BAs production strain (BL), were applied to compare the formations of volatile and non-volatile metabolite profiles according to cultivation times. In this study, histamine, putrescine, and spermidine were detected in all strains, however, 2-phenylethylamine was detected only in BH. Partial least squares discriminant analysis (PLS-DA) was applied to investigate the difference of metabolic profiles according to strains. In BH, some amino acids (phenylalanine, leucine, and threonine) and related volatile metabolites (3-methylbutanoic acid, pyrazines, styrene, and 1H-indole) were produced higher levels. On the other hand, BL produced significantly higher contents of metabolites associated with metabolism of fatty acids and nucleotides. It is necessary to consider the formation of metabolites in terms of quality as well as that of BAs during fermentation.

User engagement has been widely discussed as a critical factor for the success of digital platforms. Among the various mechanisms for increasing user engagement in digital environment, gamification as a design strategy has gained popularity over the last ten years engendering a multitude of empirical analyses. Gamification, which is the use of gamified design in non-gaming contexts, has been criticized for lacking theory driven research despite its popularity and interest from both practice and academia. This thesis addresses this research gap by providing theory-based explanations of gamification as a construct and its relationship with user engagement and free riding behaviors on digital platforms. Three interconnected essays are presented. The first essay contributes to enhancing the knowledge of gamification by creating a typology through the lens of task-technology fit that may maximize user engagement. Following this conceptual piece, the second and third essays empirically examine gamification design that structure competition and cooperation on digital platforms, respectively. These essays build upon theoretical frameworks from various disciplines including management, psychology, behavioral economics and social psychology. Through building and testing theories, this thesis improves a theoretical understanding of gamification that emphasizes human factors, which enables organizations with digital platforms to devise tailored gamification strategies that work best for them to enhance user engagement in their business.

Biogenic amines (BAs), which are mainly generated by the microbial decarboxylation of amino acids, are important nitrogen compounds in fermented foods because of their toxicology. However, amino acids, the precursors of BAs, also play an important role in generating volatile and non-volatile metabolites, which are strongly associated with quality indicators for foods. Bacillus subtilis is one of dominant fermentative microorganism in various fermented foods and is well known as a BA-producing bacterium. In this study, B. subtilis strains which have different BAs-producing capacities, higher level of BAs production strain (BH) and lower level of BAs production strain (BL), were applied to compare the formations of volatile and non-volatile metabolite profiles according to cultivation times. In this study, histamine, putrescine, and spermidine were detected in all strains, however, 2-phenylethylamine was detected only in BH. Partial least squares discriminant analysis (PLS-DA) was applied to investigate the difference of metabolic profiles according to strains. In BH, some amino acids (phenylalanine, leucine, and threonine) and related volatile metabolites (3-methylbutanoic acid, pyrazines, styrene, and 1H-indole) were produced higher levels. On the other hand, BL produced significantly higher contents of metabolites associated with metabolism of fatty acids and nucleotides. It is necessary to consider the formation of metabolites in terms of quality as well as that of BAs during fermentation.

The intensive geophysical survey of Qinghai Lake reveals the buried depth of lake sediments and their distribution features. The result indicates that there are three important interphases of Qinghai Lake sediments: T1 is the rife interphase of the lake, above which sediments are spread all over the lake basin with roughly the same thickness; T5 is the interphase from which the neotectonic sedimentary cycle begins, and its above sedimentary environment is relatively stable; Tg is the base of the lake basin. Five west-northwest (WNW) fault belts defined the tectonic structure of Qinghai Lake basin: the central hunch around Haixin Shan with two subbasins both in its north and south. The thickness of the lake sediments varies at different places, the thickest sediments are found within the two subbasins. According to the depth that the Sparker System can reach, sediment in the northern subbasin is deeper than 560 m, while sediment in the southern subbasin is deeper than 700 m. The correlation between the seismic sequence stratigraph and the lithology of onshore core shows that Qinghai Lake sediments consist of muddy silt, clay silt, silty clay, gravel silty clay, etc.

Tears have emerged as a promising alternative to blood for diagnosing diabetes. Despite increasing attempts to measure tear glucose using smart contact lenses, the controversy surrounding the correlation between tear glucose and blood glucose still limits the clinical usage of tears. Herein, we present an in-depth investigation of the correlation between tear glucose and blood glucose using a wireless and soft smart contact lens for continuous monitoring of tear glucose. This smart contact lens is capable of quantitatively monitoring the tear glucose levels in basal tears excluding the effect of reflex tears which might weaken the relationship with blood glucose. Furthermore, this smart contact lens can provide an unprecedented level of continuous tear glucose data acquisition at sub-minute intervals. These advantages allow the precise estimation of lag time, enabling the establishment of the concept called ‘personalized lag time’. This demonstration considers individual differences and is successfully applied to both non-diabetic and diabetic humans, as well as in animal models, resulting in a high correlation. The correlation between tear glucose and blood glucose is still controversial. Here, the authors demonstrated the correlation between tear glucose and blood glucose in both animal models and human subjects using smart contact lenses.

The efficient immobilization of capture antibodies is crucial for timely pathogen detection during global pandemic outbreaks. Therefore, we proposed a silica-binding protein featuring core functional domains (cSP). It comprises a peptide with a silica-binding tag designed to adhere to silica surfaces and tandem protein G fragments (2C2) for effective antibody capture. This innovation facilitates precise site-directed immobilization of antibodies onto silica surfaces. We applied cSP to silica-coated optical fibers, creating a fiber-optic biolayer interferometer (FO-BLI) biosensor capable of monitoring the monkeypox virus (MPXV) protein A29L in spiked clinical samples to rapidly detect the MPXV. The cSP-based FO-BLI biosensor for MPXV demonstrated a limit of detection (LOD) of 0.62 ng/mL in buffer, comparable to the 0.52 ng/mL LOD achieved using a conventional streptavidin (SA)-based FO-BLI biosensor. Furthermore, it achieved LODs of 0.77 ng/mL in spiked serum and 0.80 ng/mL in spiked saliva, exhibiting no cross-reactivity with other viral antigens. The MPXV detection process was completed within 14 min. We further proposed a cSP-based multi-virus biosensor strategy capable of detecting various pandemic strains, such as MPXV, the latest coronavirus disease (COVID) variants, and influenza A protein, to extend its versatility. The proposed cSP-modified FO-BLI biosensor has a high potential for rapidly and accurately detecting MPXV antigens, making valuable contributions to epidemiological studies. [Display omitted] •A recombinant protein for site-directed antibody immobilization onto silica surfaces.•A rapid and sensitive optical biosensor for detecting monkeypox virus in 14 min.•Detection of monkeypox virus in spiked clinical samples with no cross-reactivity.•A recombinant protein-based multi-virus biosensing strategy for various pandemics.