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Day-roost selection by Lasiurine tree bats during winter and their response to dormant season fires is unknown in the southeastern United States where dormant season burning is widely applied. Although fires historically were predominantly growing season, they now occur in the dormant season in this part of the Coastal Plain to support a myriad of stewardship activities, including habitat management for game species. To examine the response of bats to landscape condition and the application of prescribed fire, in the winter of 2019, we mist-netted and affixed radio-transmitters to 16 Lasiurine bats, primarily Seminole bats ( Lasiurus seminolus ) at Camp Blanding Joint Training Center in northern Florida. We then located day-roost sites to describe roost attributes. For five Seminole bats, one eastern red bat ( Lasiurus borealis ), and one hoary bat ( Lasiurus cinereus ), we applied prescribed burns in the roost area to observe bat response in real-time. Generally, Seminole bats selected day-roosts in mesic forest stands with high mean fire return intervals. At the roost tree scale, Seminole day-roosts tended to be larger, taller and in higher canopy dominance classes than surrounding trees. Seminole bats roosted in longleaf ( Pinus palustris) , slash ( Pinus elliotii ) and loblolly pine ( Pinus taeda ) more than expected based on availability, whereas sweetbay ( Magnolia virginiana ), water oak ( Quercus nigra ) and turkey oak ( Quercus laevis ), were roosted in less than expected based on availability. Of the seven roosts subjected to prescribed burns, only one male Seminole bat and one male eastern red bat evacuated during or immediately following burning. In both cases, these bats had day-roosted at heights lower than the majority of other day-roosts observed during our study. Our results suggest Seminole bats choose winter day-roosts that both maximize solar exposure and minimize risks associated with fire. Nonetheless, because selected day-roosts largely were fire-dependent or tolerant tree species, application of fire does need to periodically occur to promote recruitment and retention of suitable roost sites.

White‐nose syndrome (WNS) has caused dramatic declines of several cave‐hibernating bat species in North America since 2006, which has increased the activity of non‐susceptible species in some geographic areas or during times of night formerly occupied by susceptible species—indicative of disease‐mediated competitive release (DMCR). Yet, this pattern has not been evaluated across multiple bat assemblages simultaneously or across multiple years since WNS onset. We evaluated whether WNS altered spatial and temporal niche partitioning in bat assemblages at four locations in the eastern United States using long‐term datasets of bat acoustic activity collected before and after WNS arrival. Activity of WNS‐susceptible bat species decreased by 79–98% from pre‐WNS levels across the four study locations, but only one of our four study sites provided strong evidence supporting the DMCR hypothesis in bats post‐WNS. These results suggest that DMCR is likely dependent on the relative difference in activity by susceptible and non‐susceptible species groups pre‐WNS and the relative decline of susceptible species post‐WNS allowing for competitive release, as well as the amount of time that had elapsed post‐WNS. Our findings challenge the generality of WNS‐mediated competitive release between susceptible and non‐susceptible species and further highlight declining activity of some non‐susceptible species, especially Lasiurus borealis, across three of four locations in the eastern United States. These results underscore the broader need for conservation efforts to address the multiple potential interacting drivers of bat declines on both WNS‐susceptible and non‐susceptible species.

We report the first confirmed Myotis sodalis (Indiana Bat) maternity colony in Virginia, discovered at Fort A.P. Hill Military Reservation in Caroline County along the Piedmont-Coastal Plain Fall Line. Acoustic surveys conducted in 2014 indicated likely presence of Indiana Bats on the installation. Subsequent focal mist-netting during May–June 2015 resulted in capture of 4 lactating females that we subsequently radio tracked to a maternity colony site containing at least 20 individuals. The core roosting-area was comprised of Pinus taeda (Loblolly Pine) snags with abundant exfoliating bark and high solar exposure. This forest patch was adjacent to a large emergentshrub wetland and within a larger matrix of mature, mid-Atlantic hardwood forests. The site where we found the colony location is 140 km east of the nearest known hibernaculum and is outside of the previously documented extent of this species' occurrence.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) hybrid immunity is more protective than vaccination or previous infection alone. To investigate the kinetics of spike-reactive T (T S ) cells from SARS-CoV-2 infection through messenger RNA vaccination in persons with hybrid immunity, we identified the T cell receptor (TCR) sequences of thousands of index T S cells and tracked their frequency in bulk TCRβ repertoires sampled longitudinally from the peripheral blood of persons who had recovered from coronavirus disease 2019 (COVID-19). Vaccinations led to large expansions in memory T S cell clonotypes, most of which were CD8 + T cells, while also eliciting diverse T S cell clonotypes not observed before vaccination. TCR sequence similarity clustering identified public CD8 + and CD4 + TCR motifs associated with spike (S) specificity. Synthesis of longitudinal bulk ex vivo single-chain TCRβ repertoires and paired-chain TCRɑβ sequences from droplet sequencing of T S cells provides a roadmap for the rapid assessment of T cell responses to vaccines and emerging pathogens. Koelle and colleagues use an activation marker-dependent approach to determine the recruitment of TCR by three doses of mRNA vaccination in individuals previously infected with SARS-CoV-2.

Acoustic data are often used to describe bat activity, including habitat use within the summer reproductive period. These data inform management activities that potentially impact bats, currently a taxa of high conservation concern. To understand the relationship between acoustic and reproductive timing, we sampled big brown bats (Eptesicus fuscus) and eastern red bats (Lasiurus borealis) on 482 mist-netting and 35,410 passive acoustic sampling nights within the District of Columbia, Maryland, Pennsylvania, Virginia, and West Virginia, 2015–2018. We documented the proportion of female, pregnant, lactating, and juvenile big brown and eastern red bats within each mist-net sampling event and calculated locally estimated non-parametric scatterplot smoothing (LOESS) lines for each reproductive and acoustic dataset. We compared the peak in acoustic activity with the peaks of each reproductive condition. We determined that the highest levels of acoustic activity within the maternity season were most associated with the period wherein we captured the highest proportions of lactating bats, not juvenile bats, as often assumed.

Next-generation sequencing has enabled precision therapeutic approaches that have improved the lives of children with rare diseases. Congenital diarrhea and enteropathies (CODEs) are associated with high morbidity and mortality. Although treatment of these disorders is largely supportive, emerging targeted therapies based on genetic diagnoses include specific diets, pharmacologic treatments, and surgical interventions. We analyzed the exomes or genomes of infants with suspected monogenic congenital diarrheal disorders. Using cell and zebrafish models, we tested the effects of variants in newly implicated genes. In our case series of 129 infant probands with suspected monogenic congenital diarrheal disorders, we identified causal variants, including a new founder variant, in 62 infants (48%). Using cell and zebrafish models, we also uncovered and functionally characterized three novel genes associated with CODEs: , , and . We have characterized the broad genetic architecture of CODE disorders in a large case series of patients and identified three novel genes associated with CODEs. (Funded by the National Institutes of Health and others.).

The idea that there is a self-controlled learning advantage, where individuals demonstrate improved motor learning after exercising choice over an aspect of practice compared to no-choice groups, has different causal explanations according to the OPTIMAL theory or an information-processing perspective. Within OPTIMAL theory, giving learners choice is considered an autonomy-supportive manipulation that enhances expectations for success and intrinsic motivation. In the information-processing view, choice allows learners to engage in performance-dependent strategies that reduce uncertainty about task outcomes. To disentangle these potential explanations, we provided participants in choice and yoked groups with error or graded feedback (Experiment 1) and binary feedback (Experiment 2) while learning a novel motor task with spatial and timing goals. Across both experiments (N = 228 participants), we did not find any evidence to support a self-controlled learning advantage. Exercising choice during practice did not increase perceptions of autonomy, competence, or intrinsic motivation, nor did it lead to more accurate error estimation skills. Both error and graded feedback facilitated skill acquisition and learning, whereas no improvements from pre-test performance were found with binary feedback. Finally, the impact of graded and binary feedback on perceived competence highlights a potential dissociation of motivational and informational roles of feedback. Although our results regarding self-controlled practice conditions are difficult to reconcile with either the OPTIMAL theory or the information-processing perspective, they are consistent with a growing body of evidence that strongly suggests self-controlled conditions are not an effective approach to enhance motor performance and learning.

Instructional language is one of three techniques in OPTIMAL theory that can be manipulated to foster an autonomy-supportive practice environment to enhance motor performance and learning. While autonomy-supportive language has been shown to be beneficial in educational psychology, coaching, and health settings, the wording of task instructions has received minimal attention in the motor learning literature to date. We investigated the influence of two instructional language styles on skill acquisition in a preregistered experiment. Participants ( N = 156) learned a speed cup stacking task and received instructions throughout practice that used either autonomy-supportive or controlling language. Although the autonomy-supportive instructions resulted in higher perceptions of autonomy, there were no group differences for motor performance in acquisition or retention. Perceptions of competence and intrinsic motivation did not differ between groups at any time point. These data are difficult to reconcile with key predictions in OPTIMAL theory regarding a direct and causal influence of motivational factors on performance and learning. However, our equivalence test suggests these effects on skill acquisition may be smaller than what we were powered to detect. These findings are consistent with a growing body of evidence highlighting the need for much larger N experiments in motor learning research.

Targeted protein degradation (TPD) is an emerging therapeutic strategy that would benefit from new chemical entities with which to recruit a wider variety of ubiquitin E3 ligases to target proteins for proteasomal degradation. Here we describe a TPD strategy involving the recruitment of FBXO22 to induce degradation of the histone methyltransferase and oncogene NSD2. UNC8732 facilitates FBXO22-mediated degradation of NSD2 in acute lymphoblastic leukemia cells harboring the NSD2 gain-of-function mutation p.E1099K, resulting in growth suppression, apoptosis and reversal of drug resistance. The primary amine of UNC8732 is metabolized to an aldehyde species, which engages C326 of FBXO22 to recruit the SCF FBXO22 Cullin complex. We further demonstrate that a previously reported alkyl amine-containing degrader targeting XIAP is similarly dependent on SCF FBXO22 . Overall, we present a potent NSD2 degrader for the exploration of NSD2 disease phenotypes and a new FBXO22-recruitment strategy for TPD. Nie et al. describe a mechanism underlying the degradation of the histone methyltransferase NSD2 through the recruitment of FBXO22 E3 ligase, providing a chemical probe for NSD2 function study and targeted protein degradation.

On July 28, 2014, the Cincinnati Health Department was notified of suspected cases of foodborne botulism in two women admitted to the same hospital 12 days apart. Patient A had been treated for 12 days for suspected autoimmune disease. When patient B, the roommate of patient A, was evaluated at the same medical center for similar symptoms, it was learned that on July 13, patient A and patient B had shared a meal that included prepackaged pesto from a jar; clinicians suspected botulism and notified the local health department. The pesto had been purchased from company A's farm stand in San Clemente, California. Laboratory testing detected botulinum toxin type B by enzyme-linked immunosorbent assay (ELISA) in leftovers of pasta with pesto. A culture of these food samples yielded Clostridium spp. that produced botulinum toxin type B; polymerase chain reaction (PCR) testing also was positive for type B toxin gene. Environmental assessment of company A identified improper acidification and pressurization practices and lack of licensure to sell canned products commercially, including products in hermetically-sealed jars. On July 30, the vendor voluntarily recalled all jarred products, and the California Department of Public Health (CDPH) warned the public not to consume company A's jarred foods. This report describes the two cases and the public health investigation that traced the source of the outbreak.