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Background Mosquitoes are the deadliest organisms in the world, killing an estimated 750,000 people per year due to the pathogens they can transmit. Mosquitoes also pose a major threat to other vertebrate animals. Culex territans is a mosquito species found in temperate zones worldwide that feeds almost exclusively on amphibians and can transmit parasites; however, little is known about its ability to transmit other pathogens, including fungi. Batrachochytrium dendrobatidis (Bd) is a topical pathogenic fungus that spreads through contact. With amphibian populations around the world experiencing mass die-offs and extinctions due to this pathogen, it is critical to study all potential modes of transmission. Because Cx. territans mosquitoes are in contact with their hosts for long periods of time while blood-feeding, we hypothesize that they can transmit and pick up Bd . Methods In this study, we first assessed Cx. territans ability to transfer the fungus from an infected surface to a clean one under laboratory conditions. We also conducted a surveillance study of Bd infections in frogs and mosquitoes in the field (Mountain Lake Biological station, VA, USA). In parallel, we determined Cx. territans host preference via blood meal analysis of field caught mosquitoes. Results We found that this mosquito species can carry the fungus to an uninfected surface, implying that they may have the ability to transmit Bd to their amphibian hosts. We also found that Cx. territans feed primarily on green frogs (Rana clamitans) and bullfrogs (Rana catesbeiana) and that the prevalence of Bd within the frog population at our field site varied between years. Conclusions This study provides critical insights into understanding the role of amphibian-biting mosquitoes in transmitting pathogens, which can be applied to disease ecology of susceptible amphibian populations worldwide. Graphical Abstract

This paper engages with Isaac Levi’s approach to justifying changes in our states of full belief and offers a Peircean criticism of his strategy for resolving conflicts between the results of what inquirers deem to be the most reliable programs for a given situation and the settled beliefs about which we have no doubts. In the first section, I discuss the central features of Levi’s theory of justifying changes to our state of full belief. In the second section, I present a Peircean approach to evaluating the reliability of these programs for routine expansion of belief, and I argue that there is a conflict between Levi’s approach to situations where an inquirer has expanded her beliefs into inconsistency and Peirce’s criticisms of non-scientific methods for settling opinion. The third section presents two potential objections to the Peircean approach, objections that emphasize the importance of our concern to avoid error, and in the fourth section, I propose an original supplement to the Peircean approach that better addresses that concern. Ultimately, my aim is to develop and defend a Peircean approach that is in opposition to Levi’s views about when it is appropriate to question the reliability of our programs for routine expansion of belief but that also addresses his legitimate worries about underemphasizing our concern to avoid error.

Nonnative species are a key agent of global change. However, nonnative invertebrates remain understudied at the community scales where they are most likely to drive local extirpations. We use the North American NEON pitfall trapping network to document the number of nonnative species from 51 invertebrate communities, testing four classes of drivers. We sequenced samples using the eDNA from the sample's storage ethanol. We used AICc informed regression to evaluate how native species richness, productivity, habitat, temperature, and human population density and vehicular traffic account for continent‐wide variation in the number of nonnative species in a local community. The percentage of nonnatives varied 3‐fold among habitat types and over 10‐fold (0%–14%) overall. We found evidence for two types of constraints on nonnative diversity. Consistent with Capacity rules (i.e., how the number of niches and individuals reflect the number of species an ecosystem can support) nonnatives increased with existing native species richness and ecosystem productivity. Consistent with Establishment Rules (i.e., how the dispersal rate of nonnative propagules and the number of open sites limits nonnative species richness) nonnatives increased with automobile traffic—a measure of human‐generated propagule pressure—and were twice as common in pastures than native grasslands. After accounting for drivers associated with a community's ability to support native species (native species richness and productivity), nonnatives are more common in communities that are regularly seasonally disturbed (pastures and, potentially deciduous forests) and those experiencing more vehicular traffic. These baseline values across the US North America will allow NEON's monitoring mission to document how anthropogenic change—from disturbance to propagule transport, from temperature to trends in local extinction—further shape biotic homogenization. We use the North American NEON pitfall trapping network to document the number of nonnative species from 51 invertebrate communities, testing four classes of drivers. We found evidence for two types of constraints on nonnative diversity. Consistent with Capacity rules (i.e., how the number of niches and individuals reflect the number of species an ecosystem can support) nonnatives increased with existing native species richness and ecosystem productivity. Consistent with Establishment Rules (i.e., how the dispersal rate of nonnative propagules and the number of open sites limits nonnative species richness) nonnatives increased with automobile traffic—a measure of human‐generated propagule pressure—and were twice as common in pastures than native grasslands.

We present a method for simultaneously recording topography images and localizing specific binding sites with nm positional accuracy by combining dynamic force microscopy with single molecule recognition force spectroscopy. For this we used lysozyme adsorbed to mica, the functionality of which was characterized by enzyme immunoassays. The topography and recognition images were acquired using tips that were magnetically oscillated during scanning and contained antibodies directed against lysozyme. For cantilevers with low Q-factor (∼1 in liquid) driven at frequencies below resonance, the surface contact only affected the downward deflections (minima) of the oscillations, whereas binding of the antibody on the tip to lysozyme on the surface only affected the upwards deflections (maxima) of the oscillations. The recognition signals were therefore well separated from the topographic signals, both in space (Δ z ∼ 5 nm) and time (∼0.1 ms). Topography and recognition images were simultaneously recorded using a specially designed electronic circuit with which the maxima ( U up) and the minima ( U down) of each sinusoidal cantilever deflection period were depicted. U down was used for driving the feedback loop to record the height (topography) image, and U up provided the data for the recognition image.

Prostaglandin (PG) E2 has been linked to increased inflammation and attenuated resistance exercise adaptations in skeletal muscle. Nonaspirin cyclooxygenase (COX) inhibitors have been shown to reduce these effects. This study examined the effect of low‐dose aspirin on skeletal muscle COX production of PGE2 at rest and following resistance exercise. Skeletal muscle (vastus lateralis) biopsies were taken from six individuals (4 M/2 W) before and 3.5 hr after a single bout of resistance exercise for ex vivo PGE2 production under control and low (10 μM)‐ or standard (100 μM)‐dose aspirin conditions. Sex‐specific effects of aspirin were also examined by combining the current findings with our previous similar ex vivo skeletal muscle investigations (n = 20, 10 M/10 W). Low‐dose aspirin inhibited skeletal muscle PGE2 production (p < 0.05). This inhibition was similar to standard‐dose aspirin (p > 0.05) and was not influenced by resistance exercise (p > 0.05) (overall effect: −18 ± 5%). Men and women had similar uninhibited skeletal muscle PGE2 production at rest (men: 1.97 ± 0.33, women: 1.96 ± 0.29 pg/mg wet weight/min; p > 0.05). However, skeletal muscle of men was 60% more sensitive to aspirin inhibition than women (p < 0.05). In summary, the current findings 1) confirm low‐dose aspirin inhibits the PGE2/COX pathway in human skeletal muscle, 2) show that resistance exercise does not alter aspirin inhibitory efficacy, and 3) suggest the skeletal muscle of men and women could respond differently to long‐term consumption of low‐dose aspirin, one of the most common chronically consumed drugs in the world. Low‐dose aspirin can significantly reduce PGE2/COX pathway activity in human skeletal muscle, and this inhibitory effect of aspirin is not altered by resistance exercise. The skeletal muscle of men is significantly more sensitive than women to aspirin inhibition of this inflammation‐regulating pathway. These findings have implications for the skeletal muscle health of sedentary and resistance exercise training men and women that regularly consume low‐dose aspirin.

The Swift X-ray Telescope (XRT) has discovered that flares are quite common in early X-ray afterglows of gamma-ray bursts (GRBs), being observed in roughly 50% of afterglows with prompt follow-up observations. The flares range in fluence from a few per cent to approximately 100% of the fluence of the prompt emission (the GRB). Repetitive flares are seen, with more than four successive flares detected by the XRT in some afterglows. The rise and fall times of the flares are typically considerably smaller than the time since the burst. These characteristics suggest that the flares are related to the prompt emission mechanism, but at lower photon energies. We conclude that the most likely cause of these flares is late-time activity of the GRB central engine.

This essay discusses an alternative interpretation of the term \"Dasein\" as Heidegger uses it in Being and Time and, in particular, the possibility that Dasein is meant to contain an inherent form of intersubjectivity to which we must \"return\" in order to achieve authenticity. In doing so, I build on the work of John Haugeland and his interpretation of Dasein as a mass term, while exploring the implications such an interpretation has on Heidegger's conception of \"authenticity\". Ultimately, this paper aims to take seriously Heidegger's claim to be moving past the isolated Cartesian subject and towards a view of authentic human existence that is cognizant of the way our identities are always formed within a pre-existing community. In addition, since many interpretations of Heidegger have argued that \"the Anyone\" (Das Man) is representative of all possible forms of community, I consider how this alternative understanding of Dasein as intersubjective can shed new light on critical remarks Heidegger makes about \"the Anyone\". Thus, I argue that by reinterpreting Dasein as community, we can find more coherence between Heidegger's otherwise conflicting conceptions of authenticity and \"the Anyone\".

Mouse cancer models have provided critical insights into tumor biology; however, clinical translation of these findings has been challenging. This perspective posits that factors impacting on successful translation start with limitations in capturing human cancer pathophysiology and end with challenges in generating robust translatable preclinical end points. A comprehensive approach that considers clinically relevant mouse models with both an integrated biomarker strategy and a complementary modeling and simulation effort will strengthen the current oncology drug development paradigm. CPT Pharmacometrics Syst. Pharmacol. (2014) 3, e128; doi:10.1038/psp.2014.28; published online 06 August 2014

Mannheim's theory of self-understanding may be seen as a generative process that assimilates old unconscious assumptions and produces new replacements for them. However, difficulties arise when Mannheim tries to establish that this process leads to a more \"objective\" viewpoint for individuals. To address this, one can consider Lukács' notion that self-reflection only achieves \"objectivity\" when it changes the structure of social reality and that this is only possible when it occurs at the class level. Since Lukács explicitly connects \"objectivity\" with the ability to transform social structures, these insights relate to postmodern discussions of \"resistance\" to power such as the one found in Judith Butler's The Psychic Life of Power. Here the subversive potential of generating new possibilities remains limited when the \"subject\" is not conceived of at the class level and Lukács' theory remains helpful for avoiding fatalistic conceptions of the subject–power relation.

Cancer immunotherapy (CIT) initiates or enhances the host immune response against cancer. Following decades of development, patients with previously few therapeutic options may now benefit from CIT. Although the quantitative clinical pharmacology (qCP) of previous classes of anticancer drugs has matured during this time, application to CIT may not be straightforward since CIT acts via the immune system. Here we discuss where qCP approaches might best borrow or start anew for CIT.