Explore the vast range of titles available.

Plant cell dedifferentiation has long attracted interest as a key process for understanding the plasticity of plant development. In early studies, typical examples of plant cell dedifferentiation were described as physiological and cytological changes associated with wound healing or regenerative development. Subsequently, plant tissue and cell culture techniques, in which exciting progress was achieved after discovery of the hormonal control of cell proliferation and organogenesis in vitro in the 1950s, have been used extensively to study dedifferentiation. The pioneer studies of plant tissue/cell culture led to the hypothesis that many mature plant cells retain totipotency and related dedifferentiation to the initial step of the expression of totipotency. Plant tissue/cell cultures have provided experimental systems not only for physiological analysis, but also for genetic and molecular biological analysis, of dedifferentiation. More recently, proteomic, transcriptomic, and epigenetic analyses have been applied to the study of plant cell dedifferentiation. All of these works have expanded our knowledge of plant cell dedifferentiation, and current research is contributing to unraveling the molecular mechanisms. The present article provides a brief overview of the history of research on plant cell dedifferentiation.

Symmetry is an important component of geometric beauty and regularity in both natural and cultural scenes. Plants also display various geometric patterns with some kinds of symmetry, of which the most notable example is the arrangement of leaves around the stem, i.e., phyllotaxis. In phyllotaxis, reflection symmetry, rotation symmetry, translation symmetry, corkscrew symmetry, and/or glide reflection symmetry can be seen. These phyllotactic symmetries can be dealt with the group theory. In this review, we introduce classification of phyllotactic symmetries according to the group theory and enumerate all types of phyllotaxis, not only major ones such as spiral and decussate but also minor ones such as orixate and semi-decussate, with their symmetry groups. Next, based on the mathematical model studies of phyllotactic pattern formation, we discuss transitions between phyllotaxis types different in the symmetry class with a focus on the transition into one of the least symmetric phyllotaxis, orixate, as a representative of the symmetry-breaking process. By changes of parameters of the mathematical model, the phyllotactic pattern generated can suddenly switch its symmetry class, which is not constrained by the group-subgroup relationship of symmetry. The symmetry-breaking path to orixate phyllotaxis is also accompanied by dynamic changes of the symmetry class. The viewpoint of symmetry brings a better understanding of the variety of phyllotaxis and its transition.

Arrangement of plant leaves around the stem, termed phyllotaxis, exhibits beautiful and mysterious regularities and has been one of the most attractive subjects of biological pattern formation. After the long history of studies on phyllotaxis, it is now widely accepted that the inhibitory effect of existing leaf primordia on new primordium formation determines phyllotactic patterning. However, costoid phyllotaxis unique to Costaceae of Zingiberales, displaying spiromonostichy characterized by a steep spiral with a small divergence angle, seems to disagree with the inhibitory effect-based mechanism and has remained as a “genuine puzzle”. We developed a new mathematical model, hypothesizing that each leaf primordium exerts not only the inhibitory effect but also some inductive effect. Computer simulations with the new model successfully generated a spiromonostichous pattern when these two effects met a certain relationship. The obtained spiromonostichy matched the real costoid phyllotaxis observed with Costus megalobractea, particularly for the decrease of the divergence angle associated with the enlargement of the shoot apical meristem. The new model was also shown to be able to produce a one-sided distichous pattern that is seen in phyllotaxis of a few plants of Zingiberales and has never been addressed in the previous model studies. These results implicated inductive as well as inhibitory mechanisms in phyllotactic patterning, at least in Zingiberales.

The nucleolus is the most prominent nuclear domain, where the core processes of ribosome biogenesis occur vigorously. All these processes are finely orchestrated by many nucleolar factors to build precisely ribosome particles. In animal cells, perturbations of ribosome biogenesis, mostly accompanied by structural disorders of the nucleolus, cause a kind of cellular stress to induce cell cycle arrest, senescence, or apoptosis, which is called nucleolar stress response. The best-characterized pathway of this stress response involves p53 and MDM2 as key players. p53 is a crucial transcription factor that functions in response to not only nucleolar stress but also other cellular stresses such as DNA damage stress. These cellular stresses release p53 from the inhibition by MDM2, an E3 ubiquitin ligase targeting p53, in various ways, which leads to p53-dependent activation of a set of genes. In plants, genetic impairments of ribosome biogenesis factors or ribosome components have been shown to cause characteristic phenotypes, including a narrow and pointed leaf shape, implying a common signaling pathway connecting ribosomal perturbations and certain aspects of growth and development. Unlike animals, however, plants have neither p53 nor MDM2 family proteins. Then the question arises whether plant cells have a nucleolar stress response pathway. In recent years, it has been reported that several members of the plant-specific transcription factor family NAC play critical roles in the pathways responsive to various cellular stresses. In this mini review, we outline the plant cellular stress response pathways involving NAC transcription factors with reference to the p53-MDM2-dependent pathways of animal cells, and discuss the possible involvement of a plant-unique, NAC-mediated pathway in the nucleolar stress response in plants.

Ribosome-related mutants in Arabidopsis thaliana share several notable characteristics regarding growth and development, which implies the existence of a common pathway that responds to disorders in ribosome biogenesis. As a first step to explore this pathway genetically, we screened a mutagenized population of root initiation defective2 (rid2), a temperaturesensitive mutant that is impaired in pre-rRNA processing, and isolated suppressor of root initiation defective two1 (sriw1), a suppressor mutant in which the defects of cell proliferation observed in rid2 at the restrictive temperature was markedly rescued. sriw1 was identified as a missense mutation of the NAC transcription factor gene ANAC082. The sriw1 mutation greatly alleviated the developmental abnormalities of rid2 and four other tested ribosome-related mutants, including rid3. However, the impaired pre-rRNA processing in rid2 and rid3 was not relieved by sriw1. Expression of ANAC082 was localized to regions where phenotypic effects of ribosome-related mutations are readily evident and was elevated in rid2 and rid3 compared with the wild type. These findings suggest that ANAC082 acts downstream of perturbation of biogenesis of the ribosome and may mediate a set of stress responses leading to developmental alterations and cell proliferation defects.

Auxin-induced callus formation, known as a type of cell reprogramming, involves several transcription factors that act in lateral root initiation for plant development. Now, a new partnership of transcription factors is identified to reveal the regulatory network of auxin-induced cell reprogramming.

Plant leaves are arranged around the stem in a beautiful geometry that is called phyllotaxis. In the majority of plants, phyllotaxis exhibits a distichous, Fibonacci spiral, decussate, or tricussate pattern. To explain the regularity and limited variety of phyllotactic patterns, many theoretical models have been proposed, mostly based on the notion that a repulsive interaction between leaf primordia determines the position of primordium initiation. Among them, particularly notable are the two models of Douady and Couder (alternate-specific form, DC1; more generalized form, DC2), the key assumptions of which are that each leaf primordium emits a constant power that inhibits new primordium formation and that this inhibitory effect decreases with distance. It was previously demonstrated by computer simulations that any major type of phyllotaxis can occur as a self-organizing stable pattern in the framework of DC models. However, several phyllotactic types remain unaddressed. An interesting example is orixate phyllotaxis, which has a tetrastichous alternate pattern with periodic repetition of a sequence of different divergence angles: 180°, 90°, -180°, and -90°. Although the term orixate phyllotaxis was derived from Orixa japonica, this type is observed in several distant taxa, suggesting that it may reflect some aspects of a common mechanism of phyllotactic patterning. Here we examined DC models regarding the ability to produce orixate phyllotaxis and found that model expansion via the introduction of primordial age-dependent changes of the inhibitory power is absolutely necessary for the establishment of orixate phyllotaxis. The orixate patterns generated by the expanded version of DC2 (EDC2) were shown to share morphological details with real orixate phyllotaxis. Furthermore, the simulation results obtained using EDC2 fitted better the natural distribution of phyllotactic patterns than did those obtained using the previous models. Our findings imply that changing the inhibitory power is generally an important component of the phyllotactic patterning mechanism.

Inductive cell-cell interactions are essential for controlling cell fate determination in both plants and animals; however, the chemical basis of inductive signals in plants remains little understood. A proteoglycan-like factor named xylogen mediates local and inductive cell¡cell interactions required for xylem differentiation in Zinnia cells cultured in vitro. Here we describe the purification of xylogen and cloning of its complementary DNA, and present evidence for its role in planta. The polypeptide backbone of xylogen is a hybrid-type molecule with properties of both arabinogalactan proteins and nonspecific lipid-transfer proteins. Xylogen predominantly accumulates in the meristem, procambium and xylem. In the xylem, xylogen has a polar localization in the cell walls of differentiating tracheary elements. Double knockouts of Arabidopsis lacking both genes that encode xylogen proteins show defects in vascular development: discontinuous veins, improperly interconnected vessel elements and simplified venation. Our results suggest that the polar secretion of xylogen draws neighbouring cells into the pathway of vascular differentiation to direct continuous vascular development, thereby identifying a molecule that mediates an inductive cell-cell interaction involved in plant tissue differentiation.

About 20 years ago, molecular genetic analysis of tissue culture responses with temperature-sensitive mutants of Arabidopsis was launched in the laboratory of Prof. Atsushi Komamine as a new approach to mechanisms of plant organogenesis in vitro. In this and later studies, many interesting mutants were isolated and characterized, which led to the identification of unexpected genes as important players involved in organogenesis in vitro. The present article provides an overview of the outcomes from these molecular genetic studies derived from Komamine’s work.

Although mechanisms that activate organogenesis in plants are well established, much less is known about the subsequent fine-tuning of cell proliferation, which is crucial for creating properly structured and sized organs. Here we show, through analysis of temperature-dependent fasciation (TDF) mutants of Arabidopsis, root redifferentiation defective 1 ( rrd1 ), rrd2 , and root initiation defective 4 ( rid4 ), that mitochondrial RNA processing is required for limiting cell division during early lateral root (LR) organogenesis. These mutants formed abnormally broadened (i.e. fasciated) LRs under high-temperature conditions due to extra cell division. All TDF proteins localized to mitochondria, where they were found to participate in RNA processing: RRD1 in mRNA deadenylation, and RRD2 and RID4 in mRNA editing. Further analysis suggested that LR fasciation in the TDF mutants is triggered by reactive oxygen species generation caused by defective mitochondrial respiration. Our findings provide novel clues for the physiological significance of mitochondrial activities in plant organogenesis.