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Food security is facing a major threat from salinity and there is a need to develop salt tolerant crop varieties to ensure that the demand for food from the world’s increasing population is met. Salinity mostly occurs in arid and semi-arid regions. It may cause many adverse physiological effects on plants, i.e., toxic ion accumulation, disturbed osmotic potential, and decreased crop yield. The present study aimed to investigate the morphological, physiological, biochemical, and genetic parameters of wheat genotypes under salt stress. Six wheat genotypes were screened for salt tolerance at the seedling and maturity stage. Seeds were sown at 0 and 150 mM of salinity level. Biochemical traits, i.e., shoot/root fresh and dry weight, chlorophyll a/b and total chlorophyll contents, shoot nitrogen, shoot phosphorus, proline, and carbohydrates were measured. Wheat genotypes showed a significant increase in free amino acids, shoot nitrogen, and total soluble proteins under saline conditions. Higher Na + /K + ratio and free amino acids were estimated under 150 mM NaCl treatment in Pasban-90 and found to be the most salt-tolerant genotype. By contrast, reduced proline, total chlorophyll, and Na + /K + ratio were found in Kohistan-97 marking it to be sensitive to stress. Expression analysis of HKTs genes was performed to validate the results of two contrasting genotypes. The differential expression of HKT2; 1 and HKT2; 3 explained the tissue and genotype specific epigenetic variations. Our findings indicated that these selected genotypes can be further used for molecular studies to find out QTLs/genes related to salinity. This suggests that, in contrasting wheat genotypes, there is a differentially induced defense response to salt stress, indicating a functional correlation between salt stress tolerance and differential expression pattern in wheat.

Low temperature injury in spring has seriously destabilized the production and grain quality of common wheat. However, the molecular mechanisms underlying spring frost tolerance remain elusive. In this study, we investigated the response of a frost-tolerant wheat variety Zhongmai8444 to freezing stress at the meiotic stage. Transcriptome profiles over a time course were subsequently generated by high-throughput sequencing. Our results revealed that the prolonged freezing temperature led to the significant reductions in plant height and seed setting rate. Cell wall thickening in the vascular tissue was also observed in the stems. RNA-seq analyses demonstrated the identification of 1010 up-regulated and 230 down-regulated genes shared by all time points of freezing treatment. Enrichment analysis revealed that gene activity related to hormone signal transduction and cell wall biosynthesis was significantly modulated under freezing. In addition, among the identified differentially expressed genes, 111 transcription factors belonging to multiple gene families exhibited dynamic expression pattern. This study provided valuable gene resources beneficial for the breeding of wheat varieties with improved spring frost tolerance.

Cu2ZnSnSe4 (CZTSe) thin films were prepared by a two-step process with the electrodeposition of a Cu/Zn metallic stack precursor followed by a reactive anneal under a Se + Sn containing atmosphere. We investigate the effect of the Sex and SnSex (x = 1,2) partial pressures and annealing temperature on the morphological, structural, and elemental distribution of the CZTSe thin films. Line scanning energy dispersive spectroscopy (EDS) measurements show the presence of a Zn-rich secondary phase at the back-absorber region of the CZTSe thin films processed with higher SnSex partial pressure and lower annealing temperatures. The Zn-rich phase can be reduced by lowering the SnSex partial pressure and by increasing the annealing temperature. A very thin MoSe2 film between the CZTSe and Mo interface is confirmed by X-ray diffraction (XRD) and grazing incidence X-ray diffraction (GIXRD) measurements. These measurements indicate a strong dependence of these process variations in secondary phase formation and accumulation. A possible reaction mechanism of CZTSe thin films was presented. In a preliminary optimization of both the SnSex partial pressure and the reactive annealing process, a solar cell with 7.26% efficiency has been fabricated.

High-resolution multichannel seismic data enables the discovery of a previous, undocumented submarine canyon (Huaguang Canyon) in the Qiongdongnan Basin, northwest South China Sea. The Huaguang Canyon with a NW orientation is 140 km in length, and 2.5 km to 5 km in width in its upper reach and 4.6 km to 9.5 km in width in its lower reach. The head of the Huaguang Canyon is close to the Xisha carbonate platform and its tail is adjacent to the central canyon. This buried submarine canyon is formed by gravity flows from the Xisha carbonate platform when the sea level dropped in the early stage of the late Miocene (∼10.5 Ma). The internal architecture of the Huaguang Canyon is mainly characterized by high amplitude reflections, indicating that this ancient submarine canyon was filled with coarse-grained sediments. The sediment was principally scourced from the Xisha carbonate platform. In contrast to other buried large-scale submarine canyons (central canyon and Zhongjian Canyon) in the Qiongdongnan Basin, the Huaguang Canyon displays later formation time, smaller width and length, and single sediment supply. The coarse-grained deposits within Huaguang Canyon provide a good environment for reserving oil and gas, and the muddy fillings in Huaguang Canyon have been identified as regional caps. Therefore, Huaguang Canyon is potential area for future hydrocarbon exploration in the northwest South China Sea. Our results may contribute to a better understanding of the evolution of submarine canyons formed in carbonate environment.

High-resolution multichannel seismic data enables the discovery of a previous, undocumented submarine canyon (Huaguang Canyon) in the Qiongdongnan Basin, northwestern South China Sea. The Huaguang Canyon with a NW orientation is 140 km in length, and 2.5 km to 5 km in width in its upper reach and 4.6 km to 9.5 km in width in its lower reach. The head of the Huaguang Canyon is close to the Xisha carbonate platform and its tail is adjacent to the Central Canyon. This buried submarine canyon is formed by gravity flows from the Xisha carbonate platform when the sea level dropped in the early stage of the late Miocene (around 10.5 Ma). The internal architecture of the Huaguang Canyon is mainly characterized by high amplitude reflections, indicating that this ancient submarine canyon was filled with coarse-grained sediments. The sediment was principally scourced from the Xisha carbonate platform. In contrast to other buried large-scale submarine canyons (Central Canyon and Zhongjian Canyon) in the Qiongdongnan Basin, the Huaguang Canyon displays later formation time, smaller width and length, and single sediment supply. The coarse-grained deposits within the Huaguang Canyon provide a good environment for reserving oil and gas, and the muddy fillings in the Huaguang Canyon have been identified as regional caps. Therefore, the Huaguang Canyon is a potential area for future hydrocarbon exploration in the northwestern South China Sea. The result of this paper may contribute to a better understanding of the evolution of submarine canyons formed in carbonate environment.

Double layer distribution exists in Cu2SnZnSe4 (CZTSe) thin films prepared by selenizing the metallic precursors, which will degrade the back contact of Mo substrate to absorber layer and thus suppressing the performance of solar cell. In this work, the double‐layer distribution of CZTSe film is eliminated entirely and the formation of MoSe2 interfacial layer is inhibited successfully. CZTSe film is prepared by selenizing the precursor deposited by electrodeposition method under Se and SnSex mixed atmosphere. It is found that the insufficient reaction between ZnSe and Cu‐Sn‐Se phases in the bottom of the film is the reason why the double layer distribution of CZTSe film is formed. By increasing Sn content in the metallic precursor, thus making up the loss of Sn because of the decomposition of CZTSe and facilitate the diffusion of liquid Cu2Se, the double layer distribution is eliminated entirely. The crystallization of the formed thin film is dense and the grains go through the entire film without voids. And there is no obvious MoSe2 layer formed between CZTSe and Mo. As a consequence, the series resistance of the solar cell reduces significantly to 0.14 Ω cm2 and a CZTSe solar cell with efficiency of 7.2% is fabricated. The double layer distribution in Cu2ZnSnSe4 (CZTSe) solar cells can be eliminated entirely by increasing Sn content of the metallic precursor, and thus modifying the back contact of CZTSe solar cells and thus reducing the series resistance to a low level. The efficiency of CZTSe solar cell is improved from 5.07% with double layer distribution to 7.2% without double layer distribution.

Classification of skin lesions is a complex identification challenge. Due to the wide variety of skin lesions, doctors need to spend a lot of time and effort to judge the lesion image which zoomed through the dermatoscopy. The diagnosis which the algorithm of identifying pathological images assists doctors gets more and more attention. With the development of deep learning, the field of image recognition has made longterm progress. The effect of recognizing images through convolutional neural network models is better than traditional image recognition technology. In this work, we try to classify seven kinds of lesion images by various models and methods of deep learning, common models of convolutional neural network in the field of image classification include ResNet, DenseNet and SENet, etc. We use a fine-tuning model with a multi-layer perceptron, by training the skin lesion model, in the validation set and test set we use data expansion based on multiple cropping, and use five models’ ensemble as the final results. The experimental results show that the program has good results in improving the sensitivity of skin lesion diagnosis.

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive foliar diseases of wheat. In this study, we combined the bulked segregant RNA sequencing (BSR-seq) and comparative genomics analysis to localize the powdery mildew resistance gene in Chinese landrace Xiaomaomai. Genetic analysis of F1 plants from a crossing of Xiaomaomai × Lumai23 and the derived F2 population suggests that a single recessive gene, designated as pmXMM, confers the resistance in this germplasm. A genetic linkage map was constructed using the newly developed SNP markers and pmXMM was mapped to the distal end of chromosome 2AL. The two flanking markers 2AL15 and 2AL34 were closely linked to pmXMM at the genetic distance of 3.9 cM and 1.4 cM, respectively. Using the diagnostic primers of Pm4, we confirmed that Xiaomaomai carries a Pm4 allele and the gene function was further validated by the virus-induced gene silencing (VIGS). In addition, we systematically analyzed pmXMM in comparison with the other Pm4 alleles. The results suggest that pmXMM is identical to Pm4d and Pm4e at sequence level. Pm4b is also not different from Pm4c according to their genome/amino acid sequences. Only a few nucleotide variances were detected between pmXMM and Pm4a/b, which indicate the haplotype variation of the Pm4 gene.

This paper proposes a hybrid spectrum accessing mechanism by using NOMA-based cooperative transmission and beam-forming technology. In this mechanism, the secondary user employs spectrum-sensing technology to detect the existence of the primary user. If the primary user does not exist, the secondary source user directly transmits data to the destination user. If the primary user exists, the secondary source user finds the optimal relay according to certain selection principle before transmitting data to the destination user through the chosen relay node. For the signal receiving stage, the secondary user takes use of beam-forming technology to receive the signal from both the secondary source and the secondary relay node. Meanwhile the interference from the primary user is cancelled out in the stage. Furthermore, the outage probability for secondary user in the proposed mechanism is theoretically derived. Finally, the simulation results show that compared with the traditional mechanism, the proposed system model can not only guarantee the continuity of secondary transmission, but also significantly reduce the outage probability of secondary transmission.