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Filamentous fungi are important microorganisms used in industrial production of proteins and enzymes. Among these organisms, Trichoderma reesei, Aspergilli, and more recently Myceliophthora thermophile are the most widely used and promising ones which have powerful protein secretion capability. In recent years, there have been tremendous achievements in understanding the molecular mechanisms of the secretory pathways in filamentous fungi. The acquired pieces of knowledge can be harnessed to enhance protein production in filamentous fungi with assistance of state-of-the-art genetic engineering techniques.

Amidst global efforts toward sustainable development, this research addresses underexplored academic dimensions by evaluating the transformative potential of intelligent, sustainable architecture. Employing bibliometric techniques and Citespace 6.4.R1, we analyze two decades (2005–2024) of the Web of Science literature to identify patterns and challenges. Findings demonstrate rising scholarly output, dominated by themes like energy-efficient design, Building Information Modeling integration, and circular economy principles in urban contexts. While Europe and North America lead research activity, systemic limitations persist—including duplicated methodologies, fragmented institutional networks, and incompatible smart technologies. This study advocates for three strategic priorities: fostering interdisciplinary innovation to break homogeneity, establishing cross-sector collaboration frameworks, and accelerating industry–academia knowledge transfer. Intelligent, sustainable architecture emerges as a dual solution—technologically enabling carbon-neutral construction practices while redefining human-centric spatial quality. This dual advantage positions the International Sustainability Alliance as critical infrastructure for achieving UN Sustainable Development Goals, reconciling ecological responsibility with evolving societal demands for resilient, adaptive built environments.

Fusarium root rot, a destructive soil-borne fungal disease, necessitates eco-friendly biocontrol strategies. This study developed a microbial seed-coating approach using the antagonistic strain Paenibacillus polymyxa ZF129, formulated into a microencapsulated powder (108 CFU/g) and a suspension (CS-ZF129). CS-ZF129 application enhanced cucumber resistance, achieving 46.30 ± 0.02% disease suppression while promoting root growth. The maximum increase in the fresh weight of the root in the promotion of rectangular growth was 47.16%. The colonization dynamics of ZF129 in the rhizosphere were systematically tracked, revealing its antagonistic correlation with Fusarium proliferation. An enzymatic activity analysis further uncovered the underlying regulatory mechanisms, demonstrating induced defense responses through pathogenesis-related protein activation. These findings highlight ZF129’s dual functionality as a biocontrol agent and a plant growth promoter, offering a sustainable strategy against soil-borne pathogens.

Background Manganese peroxidase is one of the Class II fungal peroxidases that are able to oxidize the low redox potential phenolic lignin compounds. For high redox potential non-phenolic lignin degradation, mediators such as GSH and unsaturated fatty acids are required in the reaction. However, it is not known whether carboxylic acids are a mediator for non-phenolic lignin degradation. Results The white rot fungus Irpex lacteus is one of the most potent fungi in degradation of lignocellulose and xenobiotics. Two manganese peroxidases (IlMnP1 and IlMnP2) from I. lacteus CD2 were over-expressed in Escherichia coli and successfully refolded from inclusion bodies. Both IlMnP1 and IlMnP2 oxidized the phenolic compounds efficiently. Surprisingly, they could degrade veratryl alcohol, a non-phenolic lignin compound, in a Mn2+-dependent fashion. Malonate or oxalate was found to be also essential in this degradation. The oxidation of non-phenolic lignin was further confirmed by analysis of the reaction products using LC-MS/MS. We proved that Mn2+ and a certain carboxylate are indispensable in oxidation and that the radicals generated under this condition, specifically superoxide radical, are at least partially involved in lignin oxidative degradation. IlMnP1 and IlMnP2 can also efficiently decolorize dyes with different structures. Conclusions We provide evidence that a carboxylic acid may mediate oxidation of non-phenolic lignin through the action of radicals. MnPs, but not LiP, VP, or DyP, are predominant peroxidases secreted by some white rot fungi such as I. lacteus and the selective lignocellulose degrader Ceriporiopsis subvermispora. Our finding will help understand how these fungi can utilize MnPs and an excreted organic acid, which is usually a normal metabolite, to efficiently degrade the non-phenolic lignin. The unique properties of IlMnP1 and IlMnP2 make them good candidates for exploring molecular mechanisms underlying non-phenolic lignin compounds oxidation by MnPs and for applications in lignocellulose degradation and environmental remediation.

Objective Long non-coding RNA (lncRNA) expression is closely related to the pathogenesis and progression of various tumors. In this study, we investigated the mechanisms of lncRNA HOXB cluster antisense RNA 3 (HOXB-AS3), miRNA(miR)-498-5p, and disintegrin and metalloproteinase domain-containing protein 9 (ADAM9) in endometrial carcinoma (EC) cells. Methods The expression levels of lncRNA HOXB-AS3 in EC tissues and cells were detected using RT-qPCR assays. The effects of HOXB-AS3 knockdown on EC cell proliferation and apoptosis were measured using CCK-8 assays, colony formation assays, and flow cytometry. In addition, putative miR-498-5p binding sites were identified in HOXB-AS3 and ADAM9. The targeted relationships were further verified using dual-luciferase reporter and RNA pull-down assays. Results HOXB-AS3 expression was upregulated in EC tissues and cells. EC cell proliferation and viability decreased significantly in HOXB-AS3 knockdown groups. A putative miR-498-5p binding site in HOXB-AS3 was verified. Inhibition of miR-498-5p rescued the effects of HOXB-AS3 knockdown on cell proliferation and apoptosis. Finally, ADAM9 was verified as a direct target gene of miR-498-5p. Conclusions Our results suggest that lncRNA HOXB-AS3 is highly expressed in EC tissues and cells. Downregulation of HOXB-AS3 inhibits cell proliferation and promotes apoptosis in EC cells. HOXB-AS3 can upregulate ADAM9 expression by sponging miR-498-5p.

Creative Cities (CCs) have emerged as a prominent topic of global interest, representing a novel approach to urban development that fosters sustainability. This study employed the CiteSpace bibliometric tool to conduct a knowledge mapping analysis of CC research, utilizing the Web of Science (WoS) core collection as the data source spanning from 2004 to 20 April 2023. The research provides a comprehensive overview of the historical development of CCs, supported by both theoretical foundations and empirical data. Furthermore, it identifies research hotspots, core themes, and future trends within the field. A total of 2270 articles were retrieved for this study, revealing an upward trajectory in the number of articles associated with specific keywords. The research encompasses various disciplines, including geography, economics, urban studies, sociology, and art. The key focal points of CC investigations encompass sustainable development, cultural heritage, and information technology. The advancement of CCs has primarily focused on three core areas: theoretical research, policy strategies, and developmental challenges. This study underscores the importance of addressing issues such as gentrification, embracing information technology, and bridging the gap between theory and practice in CC development. The literature suggests that there is a dearth of comprehensive frameworks, spanning from theory to practice, which could serve as guiding principles and evaluation tools for the establishment and progress of CCs. This observation carries significant theoretical implications, while also offering a springboard for further exploration of the research hotspots by other scholars in the field.

Background In the biofuel industry, cellulase plays an indispensable role in hydrolyzing cellulose into fermentable glucose. Trichoderma reesei is a popular filamentous fungus with prominent ability to produce cellulase. While classical mutagenesis and modern multiplex genome engineering are both effective ways to improve cellulase production, successful obtaining of strains with improved cellulase-producing ability requires screening a large number of strains, which is time-consuming and labor intensive. Results Herein, we developed a versatile method coupling expression of the red fluorescence protein (DsRed) in T. reesei and fluorescence-assisted cell sorting (FACS) of germinated spores. This method was first established by expressing DsRed intracellularly under the control of the major cellulase cbh1 promoter in T. reesei, which allowed us to rapidly isolate cellulase hyperproducers from T. reesei progenies transformed with a dedicated transcriptional activator ace3 and from an atmospheric and room temperature plasma-created mutant T. reesei library. Since intracellularly expressed DsRed was expected to isolate mutations mainly affecting cellulase transcription, this method was further improved by displaying DsRed on the T. reesei cell surface, enabling isolation of strains with beneficial genetic alterations (overexpressing hac1 and bip1) affecting regulatory stages beyond transcription. Using this method, T. reesei cellulase hyperproducers were also successfully isolated from an Agrobacterium-mediated random insertional mutant library. Conclusions The coupled DsRed-FACS high-throughput screening method proved to be an effective strategy for fast isolation of T. reesei cellulase hyperproducers and could also be applied in other industrially important filamentous fungi.

The occurrence of diseases during greenhouse vegetable cultivation is becoming increasingly severe. Humidity and wind are important factors affecting the spread of many pathogenic fungal spores, but it remains difficult to explain the phenomenon of rapid spore spread in greenhouses. Here, the detachment of spores from hyphae during rapid drops in humidity and their subsequent dispersal due to wind is detailed. It is demonstrated that Corynespora cassiicola spores exhibit jerking movements during humidity reduction, resulting in spore discharge, and that spore connections are weaker in high‐humidity environments than in low‐humidity environments. This investigation across the fungal kingdom further reveals that jerking movements are common in the tested hyphomycete spore species. Spores rely mainly on wind to spread after being discharged from hyphae, and their spread range is influenced by factors such as wind speed, spore source height, and spore age. In summary, it is discovered that the combined effects of diurnal humidity fluctuations and wind drive the rapid spread of pathogenic spores in greenhouses, providing a theoretical basis for optimizing control strategies for airborne fungal diseases in greenhouses.

Background As global aging intensifies, issues in age-friendly cultural consumption design have become increasingly apparent. Existing age-friendly designs primarily focus on simplifying functions while neglecting cognitive fluency, resulting in the current dilemma of “high demand-low adoption.” Purpose To systematically validate the impact of cognitive fluency design, empowered by nostalgia, on age-friendly cultural consumption through a three-dimensional cognitive-physiological-psychological framework. Methods Extensive literature searches and data collection were conducted across electronic databases including Web of Science, PubMed, and CNKI. This was followed by field research and interviews using questionnaire surveys. Findings were further refined through data analysis to advance research on cognitive fluency design for aging-friendly.This study is exploratory in nature, with a relatively small sample size, and the conclusions primarily provide preliminary reference and ideas for subsequent related research. Results Findings demonstrate that in terms of emotional activation, nostalgic design effectively triggers pleasant feelings and enhances cognitive fluency among older adults. This approach yields multifaceted benefits for their physical and mental well-being, with particularly significant effects on orientation, memory, and fluency. This intervention pathway is crucial in research on cognitive fluency in aging. Conclusion Cognitive fluency design is the key variable in overcoming the ‘last mile’ of age-friendly adaptation, and it needs to be supported by nostalgia-empowerment strategies to aid contemporary age-friendly design.

Clostridium perfringens infection can induce necrotic enteritis and lead to significant economic loss to the chicken industry. In this study, a xylanase (CbXyn10C), which effectively promotes the growth of probiotics, and a protease, which degrades the biofilm of C. perfringens, were analyzed for their ability to alleviate C. perfringens-induced necrotic enteritis in broiler chickens. A total of 300 male AA chickens were divided into five treatment groups (control, no enzyme and no C. perfringens challenge; Cp, no enzyme, C. perfringens challenge; Xyn, CbXyn10C plus C. perfringens challenge; Xyn+Am, CbXyn10C+Amylase plus C. perfringens challenge; Xyn+Ap, CbXyn10C+Alkaline protease plus C. perfringens challenge). The C. perfringens CVCC 60102 was administered orally on a daily basis to the chickens from 14 to 20 days. In comparison with Cp, Xyn+Ap significantly reduced intestinal damage in the duodenum, jejunum, and ileum of chickens challenged with C. perfringens (p < 0.05). The enzymes, and particularly Xyn+Ap, notably enhanced the expression of key intestinal barrier genes, reduced the IL-6 level, and decreased the DAO (diamine oxidase) level. Not unexpectedly, feeding enzymes influenced the abundance of Lactobacillus and Butyricicoccus bacteria in the intestine. These results indicated that CbXyn10C and protease can be used to alleviate intestinal damage caused by C. perfringens infection.