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Peopling of Central Europe by Middle Pleistocene hominids is highly debatable, mainly due to the relatively harsh climatic and environmental conditions that require cultural and anatomical adjustments. At least several archaeological sites certify human occupation in the region dated back to MIS 13-11, but they represent open-air settlements. Based on the new fieldwork conducted in Tunel Wielki Cave, we can date the human occupation traces in the cave to MIS 14-12. Bipolar-on-anvil knapping technique prevails in the lithic assemblage, made exclusively in flint. The obtained results have given ground for studying the frontiers of human oikumene and the required cultural adaptive abilities.

A cave site Shelter in Smoleń III (southern Poland) contains an approximately 2-m-thick stratified sequence of Upper Pleistocene and Holocene clastic sediments, unique for Central Europe. The sequence contents abundant fossil fauna, including mollusk, rodent and bat remains. The cave sites with long profiles of subfossil fauna present a great value for reconstructions of regional terrestrial paleoenvironment. We explore the stratigraphy of this site through analyses of the lithology and geochemistry of sediments, radiocarbon dating of faunal and human remains and charcoals, and archaeological study, as well as the paleoecology derived from the taxonomic composition of fossil faunal assemblages. Our data show that the entire period of the Holocene is recorded in the rockshelter, which makes that site an exceptional and highly valuable case. We present paleoenvironmental reconstructions of regional importance, and we propose to regard Shelter in Smoleń III as a regional stratigraphic stratotype of Holocene clastic cave sediments.

Due to the long sedimentation period (about 70 000 y.) and the unique quality of the lake deposits represented by very long, monotonous layers of lacustrine chalk, the sequence at Ossówka is exceptional. We conducted highly-resolved pollen and isotope analysis of the 27-m-long, upper part of the sequence of the lake sediments covering the final stage of the Holsteinian and the early phases of the Saalian Complex (Marine Isotope Stages (MIS) 11–10). In the pollen profile three main forest interstadials (L PAZ O-3, O-5, O-7) and the intervening steppe – tundra stadials (L PAZ O-2, O-4, O-6, O-8) as well as numerous units of lower rank were identified. Interstadials were characterized mainly by the presence of well-established pine forest. Stadials in turn, represented steppe – tundra vegetation with very pronounced continental influences. Also, three clearly established phases of forest fires starting at the beginning of interstadials and gradually disappearing are interesting feature of the sequence. Fires of that scale are very rarely noted in the palynological spectra. The examined sequence is characterized by the high dynamics of changes in the post interglacial part of the profile. It provides, coupled with clear features of the Holsteinian succession and its duration, a reliable correlation with other terrestrial and marine archives.

This paper is a summary of the results of research on the accumulation conditions of the Upper Younger Loess (LMg) in Poland and Bug loess (bg) in Ukraine from the maximum stage (MIS 2) of the Vistulian (Weichselian) Glaciation in central and eastern Europe. These studies included an analysis of the morphological (topographic) situation of the loess cover, its grain size and heavy mineral composition, the preserved structures of loess sedimentation as well as mollusc and pollen analyses of this loess. They revealed that the accumulation of Upper Younger Loess (UYL) might have been more dependent on the prevailing moisture conditions than previously thought. These conditions could have been caused by cold air masses from an ice sheet and warm air masses from the Mediterranean Sea and Atlantic coming together in the Carpathians and the Holy Cross Mountains and favouring the formation of dust storms and precipitation. In this process, a loading of loess dust (formed from local rocks weathering in periglacial conditions) by atmospheric moisture particles was especially significant. The moist substrate not only favoured the periodic development of vegetation and molluscs but also enabled the interception of dust and the accumulation of an increasingly thick loess cover. Westerly and south-westerly winds predominated in the UYL as indicated by the topographic position of loess patches and the mineral composition of the studied loess. Periodically an increased air circulation from the east and northeast occurred.

The modern Nile delta developed in the Middle and Late Holocene, and at its most northern-central point is situated at the Burullus Lagoon, which is environmentally diverse, including salt marshes, mudflats, and sand plains, and separated from a sea by a sand barrier overtopped with high sand dunes. The lagoon has been fed since the Middle Holocene by the Sebennitic branch of the Nile and marine intrusions through the Bughaz inlet. A sediment core (BO-1) was collected at the northeastern shore of the lagoon and sampled at centennial scale resolution in order to reconstruct the development of the lagoon. The results show that an initial and limited lagoon had developed at the end of the Early Holocene, but after a dry period ca. 7.2 cal ka BP it has been progressively transformed into a marshy area, with occasional inflows of sea water. Lower water level and higher salinity of the Burullus Lagoon at 6.0–5.5 and 4.8–4.2 cal ka BP reflected droughts in the Nile catchment. Thereafter, the river reactivated in the Burullus Lagoon area, and since 2.8 cal ka BP was accompanied by occasional inflows of sea water. Since ca. 0.8 cal ka BP, increased fluvial activity occurred in this part of the Nile delta, which terminated after construction of the Aswan dams in the twentieth century.

In recent years, the number of metagenomic studies increased significantly. Wide range of factors, including the tremendous community complexity and variability, is contributing to the challenge in reliable microbiome community profiling. Many approaches have been proposed to overcome these problems making hardly possible to compare results of different studies. The significant differences between procedures used in metagenomic research are reflected in a variation of the obtained results. This calls for the need for standardisation of the procedure, to reduce the confounding factors originating from DNA isolation, sequencing and bioinformatics analyses in order to ensure that the differences in microbiome composition are of a true biological origin. Although the best practices for metagenomics studies have been the topic of several publications and the main aim of the International Human Microbiome Standard (IHMS) project, standardisation of the procedure for generating and analysing metagenomic data is still far from being achieved. To highlight the difficulties in the standardisation of metagenomics methods, we thoroughly examined each step of the analysis of the human gut microbiome. We tested the DNA isolation procedure, preparation of NGS libraries for next-generation sequencing, and bioinformatics analysis, aimed at identifying microbial taxa. We showed that the homogenisation time is the leading factor impacting sample diversity, with the recommendation for a shorter homogenisation time (10 min). Ten minutes of homogenisation allows for better reflection of the bacteria gram-positive/gram-negative ratio, and the obtained results are the least heterogenous in terms of beta-diversity of samples microbial composition. Besides increasing the homogenisation time, we observed further potential impact of the library preparation kit on the gut microbiome profiling. Moreover, our analysis revealed that the choice of the library preparation kit influences the reproducibility of the results, which is an important factor that has to be taken into account in every experiment. In this study, a tagmentation-based kit allowed for obtaining the most reproducible results. We also considered the choice of the computational tool for determining the composition of intestinal microbiota, with Kraken2/Bracken pipeline outperforming MetaPhlAn2 in our in silico experiments. The design of an experiment and a detailed establishment of an experimental protocol may have a serious impact on determining the taxonomic profile of the intestinal microbiome community. Results of our experiment can be helpful for a wide range of studies that aim to better understand the role of the gut microbiome, as well as for clinical purposes.

The article presents comparative characteristics of the pore structure and sorption properties of raw halloysite (R-HAL) and after calcination (C-HAL) at the temperature of 873 K. Structural parameters were determined by optical scanning and transmission electron microscopy methods as well as by mercury porosimetry (MIP, Hg) and low-pressure nitrogen adsorption (LPNA, N2, 77 K). The surface area parameter (LPNA) of halloysite mesopores before calcination was 54–61 m2/g. Calcining caused the pore surface to develop to 70–73 m2/g. The porosity (MIP) of halloysite after calcination increased from 29% to 46%, while the surface area within macropores increased from 43 m2/g to 54 m2/g. The total pore volume within mesopores and macropores increased almost twice after calcination. The course of CH4 and CO2 sorption on the halloysite was examined and sorption isotherms (0–1.5 MPa, 313 K) were determined by gravimetric method. The values of equilibrium sorption capacities increased at higher pressures. The sorption capacity of CH4 in R-HAL was 0.18 mmol/g, while in C-HAL 0.21 mmol/g. CO2 sorption capacities were 0.54 mmol/g and 0.63 mmol/g, respectively. Halloysite had a very high rate of sorption equilibrium. The values of the effective diffusion coefficient for methane on the tested halloysite were higher than De > 4.2 × 10−7 cm2/s while for carbon dioxide De > 3.1 × 10−7 cm2/s.

The article presents comparative characteristics of the pore structure and sorption properties of raw halloysite (R-HAL) and after calcination (C-HAL) at the temperature of 873 K. Structural parameters were determined by optical scanning and transmission electron microscopy methods as well as by mercury porosimetry (MIP, Hg) and low-pressure nitrogen adsorption (LPNA, N , 77 K). The surface area parameter (LPNA) of halloysite mesopores before calcination was 54-61 m /g. Calcining caused the pore surface to develop to 70-73 m /g. The porosity (MIP) of halloysite after calcination increased from 29% to 46%, while the surface area within macropores increased from 43 m /g to 54 m /g. The total pore volume within mesopores and macropores increased almost twice after calcination. The course of CH and CO sorption on the halloysite was examined and sorption isotherms (0-1.5 MPa, 313 K) were determined by gravimetric method. The values of equilibrium sorption capacities increased at higher pressures. The sorption capacity of CH in R-HAL was 0.18 mmol/g, while in C-HAL 0.21 mmol/g. CO sorption capacities were 0.54 mmol/g and 0.63 mmol/g, respectively. Halloysite had a very high rate of sorption equilibrium. The values of the effective diffusion coefficient for methane on the tested halloysite were higher than > 4.2 × 10 cm /s while for carbon dioxide > 3.1 × 10 cm /s.

Limbal epithelial stem cells (LSC, LESC) are multipotent cells used as regenerative treatment of the cornea in patients with limbal epithelial stem cell deficiency (LSCD, LESCD). There are different types of stem cell grafting including cultivated limbal epithelial transplantation (CET) and simple limbal epithelial transplantation (SLET). The outcomes of the techniques have been assessed as similar, with differences in the sample size required during the procedures. The most important culture components for stem cell cultivation include 3T3 murine fibroblasts, human amniotic membrane (HAM), fibrin gel, and culture medium. The culture medium may be enriched with serum or not; however, xenobiotic-free materials are preferred because of the low risk of pathogen transmission. Multiple studies have defined molecules important for maintaining the function of LSC including C/EBPδ, Bmi-1, p63α, interleukins (IL-6), epithelial structural proteins – keratins, and antibodies against epidermal growth factor receptor (EGFR). The cell phenotype of LSC has been described with factors of transplantation success rate such as a high percentage of p63 positive cells. The article emphasizes the role of recipient tissue preparation, modern cultivation techniques and pathophysiological processes in LSC transplantation effectiveness.