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Major depressive disorder is a devastating psychiatric disease that afflicts up to 17% of the world’s population. Postmortem brain analyses and imaging studies of patients with depression have implicated basal lateral amygdala (BLA) dysfunction in the pathophysiology of depression. However, the circuit and molecular mechanisms through which BLA neurons modulate depressive behavior are largely uncharacterized. Here, in mice, we identified that BLA cholecystokinin (CCK) glutamatergic neurons mediated negative reinforcement via D2 medium spiny neurons (MSNs) in the nucleus accumbens (NAc) and that chronic social defeat selectively potentiated excitatory transmission of the CCK BLA –D2 NAc circuit in susceptible mice via reduction of presynaptic cannabinoid type-1 receptor (CB 1 R). Knockdown of CB 1 R in the CCK BLA –D2 NAc circuit elevated synaptic activity and promoted stress susceptibility. Notably, selective inhibition of the CCK BLA –D2 NAc circuit or administration of synthetic cannabinoids in the NAc was sufficient to produce antidepressant-like effects. Overall, our studies reveal the circuit and molecular mechanisms of depression. Activating cannabinoid receptors in a newly identified neural circuit ameliorates depressive-like behaviors in mice.

Reactive oxygen species (ROS) are pivotal signaling molecules that control a variety of physiological functions. As a member of the ROS family, peroxynitrite (ONOO−) possesses strong oxidation and nitrification abilities. Abnormally elevated levels of ONOO− can lead to cellular oxidative stress, which may cause several diseases. In this work, based on the rhodamine fluorophore, we designed and synthesized a novel small-molecule fluorescent probe (DH-1) for ONOO−. Upon reaction with ONOO−, DH-1 exhibited a significant fluorescence signal enhancement (approximately 34-fold). Moreover, DH-1 showed an excellent mitochondria-targeting capability. Confocal fluorescence imaging validated its ability to detect ONOO− changes in HeLa and RAW264.7 cells. Notably, we observed the ONOO− generation during the ferroptosis process by taking advantage of the probe. DH-1 displayed good biocompatibility, facile synthesis, and high selectivity, and may have potential applications in the study of ONOO−-associated diseases in biosystems.

Cavitation is a widespread and detrimental phenomenon in hydraulic machinery, therefore, it requires to be accurately predicted. In this study, large eddy simulation (LES), scale-adaptive simulation (SAS) and grid-adaptive simulation (GAS) are employed to investigate the unsteady cavitating flow around a NACA0009 hydrofoil. The prediction accuracy of GAS, SAS, both using the shear-stress transport (SST) k — ω model as baseline turbulence model, is validated by comparing with experimental and LES results. The cavity behaviors and turbulence fields are analyzed systematically. Results show that the GAS gives a more reasonable turbulent viscosity and accurately predicts the periodic evolution of typical vortical structures of cavitating flow, such as tip leakage vortex cavitation, tip separation vortex cavitation, leading-edge cavitation, and trailing-edge vortex. The time-averaged cavity volume, volume fluctuation amplitude, and characteristic frequencies of cavities predicted by the GAS are very closed to the LES, while the SAS fails to accurately capture these cavity characteristics. Furthermore, the local trace criterion is applied to extract the vortical structures and to analyze the swirling patterns of the tip leakage vortex. Multi-scale vortical structures in LES are well identified by local trace criterion. The prediction accuracy of the SAS method for small-scale vortical structures, such as the vortex shedding on the suction side and the vortex rope around the tip leakage vortex, is obviously insufficient, while the GAS has a higher accuracy in predicting vortex shedding. The tip leakage vortex and induced vortex extracted from GAS are also closer to that of LES in both swirling patterns and scale.

In the version of this article originally published, there were several errors in Fig. 4. In Fig. 4a, the title read ‘3D repeated optical inhibition after CSDS.’ It should have read ‘3-day repeated optical inhibition after CSDS.’ In Fig. 4c, two labels that should have been aligned with the time axis appeared in the wrong place in the figure. The ticks labeled ‘SI’ and ‘Fiber implant’ should have also been labeled with ‘10’ and ‘14,’ respectively. Additionally, in Fig. 4j, a label that should have been aligned with the time axis appeared in the wrong place in the figure. The tick labeled ‘Fiber implant’ should have also been labeled with ‘14.’ The errors have been corrected in the print, PDF and HTML versions of the manuscript.

In the version of this article originally published, there were several errors in Fig. 4. In Fig. 4a, the title read '3D repeated optical inhibition after CSDS.' It should have read '3-day repeated optical inhibition after CSDS.' In Fig. 4c, two labels that should have been aligned with the time axis appeared in the wrong place in the figure. The ticks labeled 'SI' and 'Fiber implant' should have also been labeled with '10' and '14,' respectively. Additionally, in Fig. 4j, a label that should have been aligned with the time axis appeared in the wrong place in the figure. The tick labeled 'Fiber implant' should have also been labeled with '14.' The errors have been corrected in the print, PDF and HTML versions of the manuscript.

Major depressive disorder is a devastating psychiatric disease that afflicts up to 17% of the world's population. Postmortem brain analyses and imaging studies of patients with depression have implicated basal lateral amygdala (BLA) dysfunction in the pathophysiology of depression. However, the circuit and molecular mechanisms through which BLA neurons modulate depressive behavior are largely uncharacterized. Here, in mice, we identified that BLA cholecystokinin (CCK) glutamatergic neurons mediated negative reinforcement via D2 medium spiny neurons (MSNs) in the nucleus accumbens (NAc) and that chronic social defeat selectively potentiated excitatory transmission of the CCK -D2 circuit in susceptible mice via reduction of presynaptic cannabinoid type-1 receptor (CB R). Knockdown of CB R in the CCK -D2 circuit elevated synaptic activity and promoted stress susceptibility. Notably, selective inhibition of the CCK -D2 circuit or administration of synthetic cannabinoids in the NAc was sufficient to produce antidepressant-like effects. Overall, our studies reveal the circuit and molecular mechanisms of depression.

Our research focuses on solving the zero-shot text classification problem in NLP, with a particular emphasis on innovative self-training strategies. To achieve this objective, we propose a novel self-training strategy that uses labels rather than text for training, significantly reducing the model's training time. Specifically, we use categories from Wikipedia as our training set and leverage the SBERT pre-trained model to establish positive correlations between pairs of categories within the same text, facilitating associative training. For new test datasets, we have improved the original self-training approach, eliminating the need for prior training and testing data from each target dataset. Instead, we adopt Wikipedia as a unified training dataset to better approximate the zero-shot scenario. This modification allows for rapid fine-tuning and inference across different datasets, greatly reducing the time required for self-training. Our experimental results demonstrate that this method can adapt the model to the target dataset within minutes. Compared to other BERT-based transformer models, our approach significantly reduces the amount of training data by training only on labels, not the actual text, and greatly improves training efficiency by utilizing a unified training set. Additionally, our method achieves state-of-the-art results on both the Yahoo Topic and AG News datasets.

Geniposide is an iridoid glycosides purified from the fruit of Gardenia jasminoides Ellis, which is known to have antiinflammatory, anti- oxidative and anti-tumor activities. The present study aimed to investigate the effects of geniposide on experimental rat colitis and to reveal the related mechanisms, Experimental rat colitis was induced by rectal administration of a TNBS solution. The rats were treated with geniposide （25, 50 mg.kg-1.d-1, ig） or with sulfasalazine （SASP, 100 mg.kg-1.d-1, ig） as positive control for 14 consecutive days. A Caco-2 cell monolayer exposed to lipopotysaccharides （LPS） was used as an epithelial barrier dysfunction model. Transepithelial electrical resistance （TER） was measured to evaluate intestinal barrier function. In rats with TNBS-induced colitis, administration of geniposide or SASP significantly increased the TNBS-decreased body weight and ameliorated TNBS-induced experimental colitis and related symptoms. Geniposide or SASP suppressed inflammatory cytokine （TNF-α, IL-1β, and IL-6） release and neutrophil infiltration （myeloperoxidase activity） in the colon. In Caco-2 ceils, geniposide （25-100 pg/mL） ameliorated LPS-induced endothelial barrier dysfunction via dose-dependently increasing transepithelial electrical resistance （TER）. The results from both in vivo and in vitro studies revealed that geniposide down-regulated NF-KB, COX-2, iNOS and MLCK protein expression, up-regulated the expression of tight junction proteins （occludin and ZO-1）, and facilitated AMPK phosphorylation. Both AMPK siRNA transfection and AMPK overexpression abrogated the geniposide-reduced MLCK protein expression, suggesting that geniposide ameliorated barrier dysfunction via AMPK- mediated inhibition of the MLCK pathway. In conclusion, geniposide ameliorated TNBS-induced experimental rat colitis by both reducing inflammation and modulating the disrupted epithelial barrier function via activating the AMPK signaling pathway.

The perovskite structure accommodates many different combinations of elements, making it attractive for use in a wide variety of applications. Building perovskites out of only organic compounds is appealing because these materials tend to be flexible, fracture-resistant, and potentially easier to synthesize than their inorganic counterparts. Ye et al. describe a previously unknown family of all-organic perovskites, of which they synthesized 23 different family members (see the Perspective by Li and Ji). The compounds are attractive as ferroelectrics, including one compound with properties close to the well-known inorganic ferroelectric BaTiO 3 . Science , this issue p. 151 ; see also p. 132 A family of all-organic perovskites has attractive ferroelectric properties. Inorganic perovskite ferroelectrics are widely used in nonvolatile memory elements, capacitors, and sensors because of their excellent ferroelectric and other properties. Organic ferroelectrics are desirable for their mechanical flexibility, low weight, environmentally friendly processing, and low processing temperatures. Although almost a century has passed since the first ferroelectric, Rochelle salt, was discovered, examples of highly desirable organic perovskite ferroelectrics are lacking. We found a family of metal-free organic perovskite ferroelectrics with the characteristic three-dimensional structure, among which MDABCO ( N -methyl- N' -diazabicyclo[2.2.2]octonium)–ammonium triiodide has a spontaneous polarization of 22 microcoulombs per square centimeter [close to that of barium titanate (BTO)], a high phase transition temperature of 448 kelvins (above that of BTO), and eight possible polarization directions. These attributes make it attractive for use in flexible devices, soft robotics, biomedical devices, and other applications.

[Objective]In order to research on effect of different content of cottonseed meals on production performance and egg quality of Hisex laying hens. [Methods]120 Hisex laying hens of 37 weeks old are chosen,randomly divided into four groups and three repetitions are set in each group,there are 10 hens in each repetition. Group I is the control group,II,III and IV are experimental groups. Cottonseed meal contents are respectively 5%,10% and 15%,with test cycle of 10 weeks,effects of different cottonseed meal dosages on Hisex laying hens production performance and egg quality are observed. [Results]When the diet is added with 5% to 15% of cottonseed meal,the average laying rate,average egg weight and feed-gain ratio all show downward trends when compared with that of the control group,but with no significant difference（ P 〉 0. 05）; eggshell strength,eggshell thickness,Haugh unit,egg shape index all show no significant difference（ P 〉0. 05）,with the increasement of cottonseed meal,the color of egg yolk got brighter,egg yolk color of 15% added group increased by 12%,show a significant difference with that of control group（ P 〈0. 05）. [Conclusion]When the feed was added with 15% cottonseed meals,it has an unconspicuous effect on laying performance and egg quality of Hisex laying hens,but whether it had an effect on animal health and food safety or not,a further systematic research is needed.