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In recent years, the Ningbo Plain has experienced significant surface subsidence due to urbanization and industrialization, combined with the area’s unique geological and hydrological conditions. To study the surface subsidence and its causes in the Ningbo Plain, this study analyzed 166 scenes of Sentinel-1A SAR images between January 2018 and June 2023. The time series interferometric synthetic aperture radar (TS-InSAR) technique was used to acquire surface subsidence information in the area. The causes of subsidence were analyzed. The results show that: (1) the annual deformation rate of the Ningbo Plain ranges from −44 mm/yr to 12 mm/yr between 2018 and 2023. A total of 15 major subsidence zones were identified by using both the subsidence rate map and optical imagery. The most severe subsidence occurred in the northern industrial park of Cixi City, with a maximum subsidence rate of −37 mm/yr. The study reveals that the subsidence issue in the main urban area has been significantly improved compared to the 2017 subsidence data from the Ningbo Bureau of Natural Resources and Planning. However, three new subsidence areas have emerged in the main urban area, located, respectively, in Gaoqiao Town, Lishe Town, and Qiuyi Village, with maximum rates of −29 mm/year, −24 mm/year, and −23 mm/year, respectively. (2) The causes of subsidence were analyzed using various data, including land use data, geological data, groundwater-monitoring data, and transportation network data. It is found that a strong link exists between changes in groundwater levels, compressible layer thickness, and surface subsidence. The groundwater levels changes and the soft soil layer thickness are the main natural factors causing subsidence in the Ningbo Plain. Additionally, the interaction between static loads from large-scale industrial production and urban construction, along with the dynamic loads from transportation networks, contribute significantly to surface subsidence in the Ningbo Plain. The results from this study enhance the understanding of the driving factors of subsidence in the Ningbo Plain, which can provide necessary guidance for the economic development and decision-making in the region, helping to manage and potentially mitigate future subsidence issues.

Reasonable matching of capacity resources and transported cargoes is the key to realizing intelligent scheduling of less-than-truck-load (LTL) logistics. In practice, there are many types and numbers of participating objects involved in LTL logistics, such as customers, orders, trucks, unitized implements, etc. This results in a complex and large number of matching schemes where truck assignments interact with customer order service sequencing. For the truck–cargo online matching problem under real-time demand, it is necessary to comprehensively consider the online matching process of multi-node orders and the scheduling of multi-types of trucks. Combined with the actual operation scenario, a mixed-integer nonlinear programming model is introduced, and an online matching algorithm with a double-layer nested time window is designed to solve it. By solving the model in a small numerical case using Gurobi and the online matching algorithm, the validity of the model and the effectiveness of the algorithm are verified. The results indicate that the online matching algorithm can obtain optimization results with a lower gap while outperforming in terms of computation time. Relying on the realistic large-scale case for empirical analysis, the optimization results in a significant reduction in the number of trips for smaller types of trucks, and the average truck loading efficiency has reached close to 95%. The experimental results demonstrate the general applicability and effectiveness of the algorithm. Thus, it helps to realize the on-demand allocation of capacity resources and the timely response of transportation scheduling of LTL logistics hubs.

This study investigates the truck–cargo matching problem in less-than-truckload (LTL) logistics hubs, focusing on optimizing the three-dimensional loading of goods onto standardized pallets and assigning these loaded pallets to a fleet of heterogeneous vehicles. A two-stage hybrid heuristic algorithm is proposed to solve this complex logistics challenge. In the first stage, a tree search algorithm based on residual space is developed to determine the optimal layout for the 3D loading of cargo onto pallets. In the second stage, a heuristic online truck–cargo matching algorithm is introduced to allocate loaded pallets to trucks while optimizing the number of trucks used and minimizing transportation costs. The algorithm operates within a rolling time horizon, allowing it to dynamically handle real-time order arrivals and time window constraints. Numerical experiments demonstrate that the proposed method achieves high pallet loading efficiency (close to 90%), near-optimal truck utilization (nearly 95%), and significant cost reductions, making it a practical solution for real-world LTL logistics operations.

In order to extend the statistical energy analysis (SEA) method to predict the vibration and sound radiation of underwater structures, we mainly analyze the effect of water loading on the key parameters in SEA and propose the approximate expressions of modal density and mean square velocity of submerged plates. With the radiation efficiency of submerged plates previously proposed by us, the modified SEA solutions of the radiated sound power and mean square velocity of submerged stiffened rectangular plates are established. Numerical examples show that the modified SEA solutions are more close to the theoretical solutions than the present SEA solutions, and reflect the mean value or trend of the theoretical solution much better, especially at lower frequencies. An experiment of point-exciting submerged plates was done in a lake. The experiment values show that for both un-stiffened and stiffened plates, the modified SEA solutions have a good agreement while the present SEA solution has considerable error, which validates the established statistical model.

In this paper, we propose a new freight mode to describe how the designed HSR freight train serve for express delivery. We introduce the functions of the hubs and design the hybrid hub-and-spoke network of road-rail intermodal transportation from the perspective of planners, which features as single allocation rule and configures varying levels of hubs. The problem is accurately described by a mixed integer programming model with the objective to minimize total construction cost and total operation cost. We develop a hybrid heuristic algorithm based on a greedy strategy to obtain the levels of hubs, customer allocation and cargo routing. Taking the example of HSR freight network consisting of 50 cities in China, numerical experiments are conducted on the basis of forecasting data from the real-life express market to obtain the hub location schemes. The validity of the model and the performance of the algorithm are verified.

The basic (region) leucine zippers (bZIPs) are evolutionarily conserved transcription factors widely distributed in eukaryotic organisms. In plants, they are not only involved in growth and development, defense and stress responses and regulation of physiological processes but also play a pivotal role in regulating secondary metabolism. To explore the function related to the bZIP gene family in Stevia rebaudiana Bertoni, we identified 105 SrbZIP genes at the genome-wide level and classified them into 12 subfamilies using bioinformation methods. Three main classes of cis-acting elements were found in the SrbZIP promoter regions, including development-related elements, defense and stress-responsive elements and phytohormone-responsive elements. Through protein–protein interaction network of 105 SrbZIP proteins, SrbZIP proteins were mainly classified into four major categories: ABF2/ABF4/ABI5 (SrbZIP51/SrbZIP38/SrbZIP7), involved in phytohormone signaling, GBF1/GBF3/GBF4 (SrbZIP29/SrbZIP63/SrbZIP60) involved in environmental signaling, AREB3 (SrbZIP88), PAN (SrbZIP12), TGA1 (SrbZIP69), TGA4 (SrbZIP82), TGA7 (SrbZIP31), TGA9 (SrbZIP95), TGA10 (SrbZIP79) and HY5 (SrbZIP96) involved in cryptochrome signaling, and FD (SrbZIP72) promoted flowering. The transcriptomic data showed that SrbZIP genes were differentially expressed in six S. rebaudiana cultivars (‘023’, ‘110’, ‘B1188’, ‘11-14’, ‘GP’ and ‘GX’). Moreover, the expression levels of selected 15 SrbZIP genes in response to light, abiotic stress (low temperature, salt and drought), phytohormones (methyl jasmonate, gibberellic acid and salicylic acid) treatment and in different tissues were analyzed utilizing qRT-PCR. Some SrbZIP genes were further identified to be highly induced by factors affecting glycoside synthesis. Among them, three SrbZIP genes (SrbZIP54, SrbZIP63 and SrbZIP32) were predicted to be related to stress-responsive terpenoid synthesis in S. rebaudiana. The protein–protein interaction network expanded the potential functions of SrbZIP genes. This study firstly provided the comprehensive genome-wide report of the SrbZIP gene family, laying a foundation for further research on the evolution, function and regulatory role of the bZIP gene family in terpenoid synthesis in S. rebaudiana.

The basic (region) leucine zippers (bZIPs) are evolutionarily conserved transcription factors widely distributed in eukaryotic organisms. In plants, they are not only involved in growth and development, defense and stress responses and regulation of physiological processes but also play a pivotal role in regulating secondary metabolism. To explore the function related to the bZIP gene family in Stevia rebaudiana Bertoni, we identified 105 SrbZIP genes at the genome-wide level and classified them into 12 subfamilies using bioinformation methods. Three main classes of cis-acting elements were found in the SrbZIP promoter regions, including development-related elements, defense and stress-responsive elements and phytohormone-responsive elements. Through protein–protein interaction network of 105 SrbZIP proteins, SrbZIP proteins were mainly classified into four major categories: ABF2/ABF4/ABI5 (SrbZIP51/SrbZIP38/SrbZIP7), involved in phytohormone signaling, GBF1/GBF3/GBF4 (SrbZIP29/SrbZIP63/SrbZIP60) involved in environmental signaling, AREB3 (SrbZIP88), PAN (SrbZIP12), TGA1 (SrbZIP69), TGA4 (SrbZIP82), TGA7 (SrbZIP31), TGA9 (SrbZIP95), TGA10 (SrbZIP79) and HY5 (SrbZIP96) involved in cryptochrome signaling, and FD (SrbZIP72) promoted flowering. The transcriptomic data showed that SrbZIP genes were differentially expressed in six S. rebaudiana cultivars (‘023’, ‘110’, ‘B1188’, ‘11-14’, ‘GP’ and ‘GX’). Moreover, the expression levels of selected 15 SrbZIP genes in response to light, abiotic stress (low temperature, salt and drought), phytohormones (methyl jasmonate, gibberellic acid and salicylic acid) treatment and in different tissues were analyzed utilizing qRT-PCR. Some SrbZIP genes were further identified to be highly induced by factors affecting glycoside synthesis. Among them, three SrbZIP genes (SrbZIP54, SrbZIP63 and SrbZIP32) were predicted to be related to stress-responsive terpenoid synthesis in S. rebaudiana. The protein–protein interaction network expanded the potential functions of SrbZIP genes. This study firstly provided the comprehensive genome-wide report of the SrbZIP gene family, laying a foundation for further research on the evolution, function and regulatory role of the bZIP gene family in terpenoid synthesis in S. rebaudiana.

为了解栖息地和气候变化对北树鼩 (Tupaia belangeri) 活动的影响, 于2012年8月—2013年7月采用红外相机技术对广西弄岗国家级自然保护区15 hm2森林动态监测样地及其周边区域内的北树鼩进行监测, 采用核密度估计、重叠指数及相对活动强度指数分析其日活动节律及季节性变化规律。结果表明：北树鼩的日活动节律模式属于晨昏活动类型 (日活动高峰期：07：00—08：00、16：00—17：00) 。不同月份间北树鼩的活动强度指数 (t=13.072, df=11, P<0.001) 和旱雨季间的日活动节律重叠指数 (Δ=0.83, P<0.01) 都存在极显著差异, 旱季上午的活动峰值比雨季延迟1 h出现, 而下午的活动峰值却比雨季提前1 h出现。雨季的活动时间 (11.24±0.68) s长于旱季 (9.84±0.58) s。

Single-cell analysis is a valuable tool for dissecting cellular heterogeneity in complex systems 1 . However, a comprehensive single-cell atlas has not been achieved for humans. Here we use single-cell mRNA sequencing to determine the cell-type composition of all major human organs and construct a scheme for the human cell landscape (HCL). We have uncovered a single-cell hierarchy for many tissues that have not been well characterized. We established a ‘single-cell HCL analysis’ pipeline that helps to define human cell identity. Finally, we performed a single-cell comparative analysis of landscapes from human and mouse to identify conserved genetic networks. We found that stem and progenitor cells exhibit strong transcriptomic stochasticity, whereas differentiated cells are more distinct. Our results provide a useful resource for the study of human biology. Single-cell RNA sequencing is used to generate a dataset covering all major human organs in both adult and fetal stages, enabling comparison with similar datasets for mouse tissues.

With the prevalence of pre-trained language models (PLMs) and the pre-training–fine-tuning paradigm, it has been continuously shown that larger models tend to yield better performance. However, as PLMs scale up, fine-tuning and storing all the parameters is prohibitively costly and eventually becomes practically infeasible. This necessitates a new branch of research focusing on the parameter-efficient adaptation of PLMs, which optimizes a small portion of the model parameters while keeping the rest fixed, drastically cutting down computation and storage costs. In general, it demonstrates that large-scale models could be effectively stimulated by the optimization of a few parameters. Despite the various designs, here we discuss and analyse the approaches under a more consistent and accessible term ‘delta-tuning’, where ‘delta’ a mathematical notation often used to denote changes, is borrowed to refer to the portion of parameters that are ‘changed’ during training. We formally describe the problem and propose a unified categorization criterion for existing delta-tuning methods to explore their correlations and differences. We also discuss the theoretical principles underlying the effectiveness of delta-tuning and interpret them from the perspectives of optimization and optimal control. Furthermore, we provide a holistic empirical study on over 100 natural language processing tasks and investigate various aspects of delta-tuning. With comprehensive study and analysis, our research demonstrates the theoretical and practical properties of delta-tuning in the adaptation of PLMs. Training a deep neural network can be costly but training time is reduced when a pre-trained network can be adapted to different use cases. Ideally, only a small number of parameters needs to be changed in this process of fine-tuning, which can then be more easily distributed. In this Analysis, different methods of fine-tuning with only a small number of parameters are compared on a large set of natural language processing tasks.