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The heavily human-perturbed coastal oceans are hotspots of nitrous oxide (N 2 O) emission to the atmosphere. The processes underpinning the N 2 O flux, however, remain poorly understood, leading to large uncertainties in assessing global N 2 O budgets. Using a suite of nitrogen isotope labeling experiments, we show that multiple processes contribute to N 2 O production throughout the estuarine-coastal gradient, sustaining intensive N 2 O flux to the atmosphere. Unexpectedly, denitrification, rather than ammonia oxidation as previously assumed, constitutes the major source of N 2 O in well-oxygenated coastal waters. Size-fractionated manipulation experiments with gene analysis further reveal niche partitioning of ammonia oxidizers and denitrifiers across the particle size spectrum; denitrification dominated on large particles and ammonia oxidizers on small particles. Total N 2 O production rate increases with substrate and particle concentrations, suggesting a crucial interplay between nutrients and particles in controlling N 2 O production. The controlling factors identified here may help understand climate feedback mechanisms between human activity and coastal oceans. Incomplete denitrification associated with the micro-niche of marine particles, instead of nitrification as previously assumed, is a major source of N 2 O in the turbid coastal waters, even though the water is well-oxygenated.

Cardiac neural crest cells contribute to important portions of the cardiovascular system including the aorticopulmonary septum and cardiac ganglion. Using replication incompetent avian retroviruses for precise high-resolution lineage analysis, we uncover a previously undescribed neural crest contribution to cardiomyocytes of the ventricles in Gallus gallus, supported by Wnt1-Cre lineage analysis in Mus musculus. To test the intriguing possibility that neural crest cells contribute to heart repair, we examined Danio rerio adult heart regeneration in the neural crest transgenic line, Tg(−4.9sox10:eGFP). Whereas the adult heart has few sox10+ cells in the apex, sox10 and other neural crest regulatory network genes are upregulated in the regenerating myocardium after resection. The results suggest that neural crest cells contribute to many cardiovascular structures including cardiomyocytes across vertebrates and to the regenerating heart of teleost fish. Thus, understanding molecular mechanisms that control the normal development of the neural crest into cardiomyocytes and reactivation of the neural crest program upon regeneration may open potential therapeutic approaches to repair heart damage in amniotes. Before birth, unspecialized stem cells go through a process called differentiation to form the many types of cells found in the adult. Neural crest cells are a group of these stem cells found in all animals with backbones (i.e. vertebrates) including humans. These cells migrate extensively during development to form many different parts of the body. Due to their contributions to diverse organs and tissues, neural crest cells are very important for healthy development. The heart ventricle is one of the tissues to which neural crest cells contribute during embryonic development in fish and amphibians. However, it was unclear whether this is also the case for birds or mammals or whether neural crest cells have any roles in the regeneration of the adult heart after injury in fish and amphibians. To address these questions, Tang, Martik et al. used cell biology techniques to track neural crest cells in living animals. The experiments revealed that neural crest cells contribute to heart tissue in developing birds and mammals and help repair the heart in adult zebrafish. Further results showed that the contribution of neural crest cells to the heart is controlled by the same genes during both the growth of the embryonic heart and the repair of the adult heart. These results provide new insights into the repair and healing of damaged heart muscle in fish. They also show that similar processes could exist in mammals, including humans, suggesting that activating neural crest cells in the heart could treat damage caused by heart attacks and related conditions.

Neural crest stem cells arising from caudal hindbrain (often called cardiac and posterior vagal neural crest) migrate long distances to form cell types as diverse as heart muscle and enteric ganglia, abnormalities of which lead to common congenital birth defects. Here, we explore whether individual caudal hindbrain neural crest precursors are multipotent or predetermined toward these particular fates and destinations. To this end, we perform lineage tracing of chick neural crest cells at single-cell resolution using two complementary approaches: retrovirally mediated multiplex clonal analysis and single-cell photoconversion. Both methods show that the majority of these neural crest precursors are multipotent with many clones producing mesenchymal as well as neuronal derivatives. Time-lapse imaging demonstrates that sister cells can migrate in distinct directions, suggesting stochasticity in choice of migration path. Perturbation experiments further identify guidance cues acting on cells in the pharyngeal junction that can influence this choice; loss of CXCR4 signaling results in failure to migrate to the heart but no influence on migration toward the foregut, whereas loss of RET signaling does the opposite. Taken together, the results suggest that environmental influences rather than intrinsic information govern cell fate choice of multipotent caudal hindbrain neural crest cells. Neural crest stem cells formed from the caudal hindbrain migrate long distances to the heart and gut, but how cell fate is determined is unclear. Here, the authors use multiplex clonal analysis and single-cell photoconversion lineage tracing to show environmental not intrinsic factors affect the cell fate of multipotent caudal hindbrain cells in the chick.

Growth of the prominent nitrogen-fixing cyanobacterium Trichodesmium is often limited by phosphorus availability in the ocean. How nitrogen fixation by phosphorus-limited Trichodesmium may respond to ocean acidification remains poorly understood. Here, we use phosphate-limited chemostat experiments to show that acidification enhanced phosphorus demands and decreased phosphorus-specific nitrogen fixation rates in Trichodesmium . The increased phosphorus requirements were attributed primarily to elevated cellular polyphosphate contents, likely for maintaining cytosolic pH homeostasis in response to acidification. Alongside the accumulation of polyphosphate, decreased NADP(H):NAD(H) ratios and impaired chlorophyll synthesis and energy production were observed under acidified conditions. Consequently, the negative effects of acidification were amplified compared to those demonstrated previously under phosphorus sufficiency. Estimating the potential implications of this finding, using outputs from the Community Earth System Model, predicts that acidification and dissolved inorganic and organic phosphorus stress could synergistically cause an appreciable decrease in global Trichodesmium nitrogen fixation by 2100. How reduced seawater pH and increased carbon dioxide might affect the prominent nitrogen fixer Trichodesmium in phosphorus-limited oceans is poorly understood. This study used phosphate-limited chemostat experiments to show that Trichodesmium may fix less nitrogen for a given amount of phosphorus at low pH. Thus, marine productivity is likely to decline in a future, more acidic ocean.

During development, much of the enteric nervous system (ENS) arises from the vagal neural crest that emerges from the caudal hindbrain and colonizes the entire gastrointestinal tract. However, a second ENS contribution comes from the sacral neural crest that arises in the caudal neural tube and populates the post-umbilical gut. By coupling single-cell transcriptomics with axial-level-specific lineage tracing in avian embryos, we compared the contributions of embryonic vagal and sacral neural crest cells to the chick ENS and the associated peripheral ganglia (Nerve of Remak and pelvic plexuses). At embryonic day (E) 10, the two neural crest populations form overlapping subsets of neuronal and glia cell types. Surprisingly, the post-umbilical vagal neural crest much more closely resembles the sacral neural crest than the pre-umbilical vagal neural crest. However, some differences in cluster types were noted between vagal and sacral derived cells. Notably, RNA trajectory analysis suggests that the vagal neural crest maintains a neuronal/glial progenitor pool, whereas this cluster is depleted in the E10 sacral neural crest which instead has numerous enteric glia. The present findings reveal sacral neural crest contributions to the hindgut and associated peripheral ganglia and highlight the potential influence of the local environment and/or developmental timing in differentiation of neural crest-derived cells in the developing ENS.

Znf703 is an RAR- and Wnt-inducible transcription factor that exhibits a complex expression pattern in the developing embryo: Znf703 mRNA is found in the early circumblastoporal ring, then later throughout the neural plate and its border, and subsequently in the mid/hindbrain and somites. We show that Znf703 has a different and separable function in early mesoderm versus neural crest and placode development. Independent of its early knockdown phenotype on Gdf3 and Wnt8 , Znf703 disrupts patterning of distinct neural crest migratory streams normally delineated by Sox10, Twist , and Foxd3 and inhibits otocyst formation and otic expression of Sox10 and Eya1 . Furthermore, Znf703 promotes massive overgrowth of SOX2+ cells, disrupting the SoxB1 balance at the neural plate border. Despite prominent expression in other neural plate border-derived cranial and sensory domains, Znf703 is selectively absent from the otocyst, suggesting that Znf703 must be specifically cleared or down-regulated for proper otic development. We show that mutation of the putative Groucho-repression domain does not ameliorate Znf703 effects on mesoderm, neural crest, and placodes. We instead provide evidence that Znf703 requires the Buttonhead domain for transcriptional repression.

Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N 2 ) fixing marine diazotrophs, knowledge gaps remain regarding ecological interactions between diazotrophs and other community members. Using quantitative 16S and 18S V4 rDNA amplicon sequencing, we surveyed eukaryotic and prokaryotic microbial communities from samples collected in August 2016 and 2017 across the Western North Atlantic. Leveraging and significantly expanding an earlier published 2015 molecular dataset, we examined microbial community structure and ecological co-occurrence relationships associated with intense hotspots of N 2 fixation previously reported at sites off the Southern New England Shelf and Mid-Atlantic Bight. Overall, we observed a negative relationship between eukaryotic diversity and both N 2 fixation and net community production (NCP). Maximum N 2 fixation rates occurred at sites with high abundances of mixotrophic stramenopiles, notably Chrysophyceae . Network analysis revealed such stramenopiles to be keystone taxa alongside the haptophyte diazotroph host Braarudosphaera bigelowii and chlorophytes. Our findings highlight an intriguing relationship between marine stramenopiles and high N 2 fixation coastal sites.

The realization of a controllable transparent conducting system with selective light transparency is crucial for exploring many of the most intriguing effects in top‐illuminated optoelectronic devices. However, the performance is limited by insufficient electrical conductivity, low work function, and vulnerable interface of traditional transparent conducting materials, such as tin‐doped indium oxide. Here, it is reported that two‐dimensional (2D) titanium carbide (Ti3C2Tx) MXene film acts as an efficient transparent conducting electrode for the lead sulfide (PbS) colloidal quantum dots (CQDs) photodiode with controllable near infrared transmittance. The solution‐processed interface engineering of MXene and PbS layers remarkably reduces the interface defects of MXene/PbS CQDs and the carrier concentration in the PbS layer. The stable Ti3C2Tx/PbS CQDs photodiodes give rise to a high specific detectivity of 5.51 × 1012 cm W−1 Hz1/2, a large dynamic response range of 140 dB, and a large bandwidth of 0.76 MHz at 940 nm in the self‐powered state, ranking among the most exceptional in terms of comprehensive performance among reported PbS CQDs photodiodes. In contrast with the traditional photodiode technologies, this efficient and stable approach opens a new horizon to construct widely used infrared photodiodes with CQDs and MXenes. A novel two‐dimensional titanium carbide (Ti3C2Tx) MXene film is introduced as an effective transparent conducting electrode in lead sulfide quantum dot photodiodes. Exhibiting exceptional near‐infrared transmittance control, interface engineering enhances performance, yielding high detectivity, extensive dynamic response, and notable bandwidth, thereby advancing infrared photodiode technology.

Biological nitrogen fixation is an important source of new nitrogen, influencing ocean fertility and carbon uptake. While recently documented in Arctic waters, its role in the Southern Ocean remains uncertain. We measured nitrogen fixation along the Western Antarctic Peninsula and at Palmer Station over two austral summer months. Rates from 15 N 2 assay were below conservative detection limits but detectable under less stringent detection thresholds. Continuous acetylene reduction assay provided further support. nifH gene sequencing identified Gammaproteobacteria as the dominating identified diazotrophs, while Epsilonproteobacteria contributed disproportionally to nifH expression when putative nitrogen fixation was highest. Combined with environmental observations, we hypothesize that vertical water mixing resuspended sediments into the water column and contributed to the limited nitrogen fixation. Given the sporadic and low rates, further research is needed to determine whether nitrogen fixation plays a minor role or represents an overlooked process with biogeochemical significance in the Southern Ocean. Low levels of nitrogen fixation may be occurring in the Southern Ocean, according to in-situ measurements of seawater from the western Antarctic Peninsula and Palmer Station

In the wind energy generation system, the brushless doubly-fed induction machine (BDFIM) has shown significant application potential, since it eliminates the electric brush and slip ring. However, the complicated rotor structure increases the control difficulty, especially resulting in complicated coupled terms in the current sub-system, which deteriorates the dynamic performance and reduces the system robustness. In order to address the problems caused by complex coupled terms, an internal model current control strategy is presented for the BDFIM, and an active damping term is designed for suppressing the disturbance caused by the total resistance. The proposed method simplifies the controller parameters design, and it achieves the fast-dynamic response and the good tracking performance, as well as good robustness. On the other hand, the feedforward term composed by the grid voltage is added to the internal model controller in order to suppress the disturbance when the symmetrical grid voltage sag happens. Finally, the simulation and experimental results verify the feasibility and effectiveness of the proposed method.