Explore the vast range of titles available.

The increasing incidence and mortality of malignant tumors have required the development of diversified innovative therapies. Compared with the new therapeutic molecule invention, it is a more economical and efficient way to reposition old drugs that have been marketed or in clinical trials for tumor treatment. Studies have been carried out for repositioning the old approval drugs that are widely applied clinically with reliable biosafety and effectiveness evidence, including aspirin, artemisinin (and its derivatives, eg, dihydroartemisinin), metformin, and disulfiram (DSF). In addition to the approved pharmacological activity, these old drugs exhibited broad tumor‐suppressive effects. This review summarizes the drug repositioning strategy of DSF through nanotechnology and outlines the DSF nano‐formulations for cancer targeting delivery and therapy. Repurposing disulfiram for cancer therapy has attracted great interest. By using advanced delivery techniques, DSF‐based nanomedicines can achieve tumor‐targeted delivery and controlled release, thus providing a promising solution to enhance treatment efficacy. Nanomedicine‐mediated codelivery also offers a useful strategy for DSF‐based combination therapy. Many efforts have been made to repurposing disulfiram due to the extensive anti‐tumor activity and biosafety. By using advanced delivery techniques, DSF‐based nanomedicine would achieve targeted delivery and spatiotemporal controlled release, thus providing a potential solution to address the 3R principle (right place, right dose, and right time) to maximize the efficacy of cancer therapy.

Dysregulated mucosal immune responses and colonic fibrosis impose two formidable challenges for ulcerative colitis treatment. It indicates that monotherapy could not sufficiently deal with this complicated disease and combination therapy may provide a potential solution. A chitosan-modified poly(lactic-co-glycolic acid) nanoparticle (CS-PLGA NP) system was developed for co-delivering patchouli alcohol and simvastatin to the inflamed colonic epithelium to alleviate the symptoms of ulcerative colitis via remodeling immune microenvironment and anti-fibrosis, a so-called “two-birds-one-stone” nanotherapeutic strategy. The bioadhesive nanomedicine enhanced the intestinal epithelial cell uptake efficiency and improved the drug stability in the gastrointestinal tract. The nanomedicine effectively regulated the Akt/MAPK/NF-κB pathway and reshaped the immune microenvironment through repolarizing M2Φ, promoting regulatory T cells and G-MDSC, suppressing neutrophil and inflammatory monocyte infiltration, as well as inhibiting dendritic cell maturation. Additionally, the nanomedicine alleviated colonic fibrosis. Our work elucidates that the colon-targeted codelivery for combination therapy is promising for ulcerative colitis treatment and to address the unmet medical need. Graphical Abstract

Background Stenotrophomonas maltophilia ( S. maltophilia ) is an emerging global multiple-drug-resistant organism. It becomes increasingly challenging to treat S. maltophilia infection effectively. Novel therapeutic and preventive approaches targeting S. maltophilia infection are still lacking. This study aims to isolate outer membrane proteins (Omps) from S. maltophilia and use immunoproteomic technology to identify potential vaccine candidates of Omps against S. maltophilia infections. Methods Omps from S. maltophilia culture were separated by two-dimensional electrophoresis and identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry and nano liquid chromatography coupled fourier transform ion cyclotron resonance tandem mass spectrometry. Recombinant Omps were prepared and used to immunize mice, and the potency of mouse anti-Omp serum was tested in opsonophagocytic killing assay (OPKA). The effects of immunization with recombinant Omp on blood and tissue bacterial loads in a mouse model of S. maltophilia -induced infection were analyzed. Results Outer membrane protein A (OmpA) and Smlt4123 were identified by mass spectrometry. Mouse anti-Smlt4123 serum significantly reduced the bacterial counts in healthy individuals’ blood in OPKA ( P  < 0.05) but mouse anti-OmpA serum did not. Enzyme-linked immunosorbent assay revealed that the antibody subtype of mouse anti-Smlt4123 antibody was IgG1. Eight hours after an intraperitoneal challenge with S. maltophilia , the bacterial loads in mouse blood were significantly lower in the mice receiving immunization with recombinant Smlt4123 than in the control mice receiving no immunization ( P  < 0.05), whereas the bacterial loads in other organs, such as the liver, spleen, lung, and kidney were similar in the two groups. Conclusions The results revealed that the immunoproteomic approach was an efficient way to screen the immunogenic protein of Stenotrophomonas maltophilia . Moreover, the recombinant Smlt4123 had potential to protect mice from bacteremia caused by S. maltophilia in the early stages.

Stenotrophomonas maltophilia (S. maltophilia), a multi-drug resistant opportunistic pathogen, is associated with nosocomial and community-acquired infections. Preventive and therapeutic strategies for such infections are greatly needed. In this study, sequence alignment analysis revealed that Outer membrane protein A (OmpA) was highly conserved among S. maltophilia strains but shared no significant similarity with human and mouse proteomes. In mice, intranasal immunization with S. maltophilia recombinant OmpA (rOmpA) without additional adjuvant induced sustained mucosal and systemic rOmpA-specific antibody responses. Treatment with rOmpA stimulated significantly higher levels of secretion of IFN-γ, IL-2, and IL-17A (All P<0.05) from the primary splenocytes isolated from rOmpA-immunized mice than from the primary splenocytes isolated from PBS-immunized mice. Furthermore, mice immunized with rOmpA showed significantly reduced bacterial burden in the lung and reduced levels of pro-inflammatory cytokines (TNF-α and IL-6) in bronchoalveolar lavage fluid (BALF) 24 hours after intranasal S. maltophilia infection, indicating that immunization with rOmpA may have protective effects against S. maltophilia challenge in mice. Our findings suggest that intranasal immunization with rOmpA may induce mucosal and systemic immune responses in mice, trigger Th1- and Th17-mediated cellular immune responses, and thus stimulate host immune defense against S. maltophilia infection. These results also demonstrate that intranasal vaccination may offer an alternative approach to current strategies since it induces a mucosal as well as a systemic immune response.

Facile synthesis of zero-valent iron nanoparticles has been developed using Tie Guanyin tea extract as reducing and stabilizing agent. The characterization carried out by UV-Vis, SEM, TEM, XRD, and FTIR techniques has identified the successful synthesis of the zero-valent iron nanoparticles. It is evident from the TEM result that spherical zero-valent iron nanoparticles with average size of 6.58±0.76 nm have been obtained through biological method in this study. FTIR spectrum demonstrates that the polyphenols play an important role in the synthetic process. Diffraction peak at 2θ of 44.9° and 49.1° in XRD spectrum explains the existence of the iron nanoparticles. Additionally, effect of concentration of iron nanoparticles and concentration of bromothymol blue on the kinetic rate constants during the degradation process was studied.

Micro-expression recognition (MER) is challenged by a brief duration, low intensity, and heterogeneous spatial frequency patterns. This study introduces a novel MER architecture that reduces computational cost by fine-tuning a large feature extraction model with LoRA, while integrating frequency-domain transformation and graph-based temporal modeling to minimize preprocessing requirements. A Spatial Frequency Adaptive (SFA) module decomposes high- and low-frequency information with dynamic weighting to enhance sensitivity to subtle facial texture variations. A Dynamic Graph Attention Temporal (DGAT) network models video frames as a graph, combining Graph Attention Networks and LSTM with frequency-guided attention for temporal feature fusion. Experiments on the SAMM, CASME II, and SMIC datasets demonstrate superior performance over existing methods. On the SAMM 5-class setting, the proposed approach achieves an unweighted F1 score (UF1) of 81.16% and an unweighted average recall (UAR) of 85.37%, outperforming the next best method by 0.96% and 2.27%, respectively.

Existing video inpainting methods typically combine optical flow propagation with Transformer architectures, achieving promising inpainting results. However, they lack adaptive inpainting strategy optimization in diverse scenarios, and struggle to capture high-level temporal semantics, causing temporal inconsistencies and quality degradation. To address these challenges, we make one of the first attempts to introduce reinforcement learning into the video inpainting domain, establishing a closed-loop framework named CLIP-RL that enables adaptive strategy optimization. Specifically, video inpainting is reformulated as an agent–environment interaction, where the inpainting module functions as the agent’s execution component, and a pre-trained inpainting detection module provides real-time quality feedback. Guided by a policy network and a composite reward function that incorporates a weighted temporal alignment loss, the agent dynamically selects actions to adjust the inpainting strategy and iteratively refines the inpainting results. Compared to ProPainter, CLIP-RL improves PSNR from 34.43 to 34.67 and SSIM from 0.974 to 0.986 on the YouTube-VOS dataset. Qualitative analysis demonstrates that CLIP-RL excels in detail preservation and artifact suppression, validating its superiority in video inpainting tasks.

Aluminum-based P-inactivation agent (Al-PIA) is a highly effective material for phosphorus removal, which has great potential to restore eutrophic water bodies. In this study, active capping plate (ACP) was formed by mixing Al-PIA with cement and fly ash. The scour resistance coefficient of the prepared ACP was 98.01 % and the water permeability coefficient was 0.139 cm/s, which meet the requirements of strength and water permeability. Exogenous phosphorus was used as an input in the indoor dynamic experiment to examine the effectiveness of ACP capping to control phosphorus in sediment-water system. The indoor dynamic experiment lasted for 110 days. After experiment, the effect of dissolved oxygen (DO) and pH was explored, the change of phosphorus concentration in the overlying water was examined, and the changes of phosphorus in ACP and sediments were also analyzed. After ACP capping, the pH eventually maintained below 8 and was slightly higher than that of the control system. The DO concentration initially decreased, then increased, and finally remained in the range of 7 to 8 mg/L. Compared to the control system, the average reduction rates of total phosphorus (TP), dissolve total phosphorus (DTP) and dissolve inorganic phosphorus (DIP) in the overlying water were 90.57%, 91.69%, and 92.80%, respectively. ACP promotes the conversion of unstable phosphorus in sediments to stable phosphorus, and calcium-bound phosphorus (Ca-P) is the main form of phosphorus fixed by ACP. In addition, ACP was found to have a limited effect on reducing the risk of release of biologically available phosphorus from sediments.

A biological aluminum–based P-inactivation agent (BA-PIA) has been developed and demonstrated to effectively remove nitrogen and phosphorus; however, whether it can control the release of nitrogen and phosphorus in sediment still needs study. This study aimed to examine the effect of BA-PIA on controlling sediment nitrogen and phosphorus release. BA-PIA was prepared by artificial aeration. The use of BA-PIA in controlling nitrogen and phosphorus release was studied using water and sediment from a landscape lake in static simulation experiments. The sediment microbial community was analyzed using high-throughput sequencing. Static simulation showed that the reduction rates of total nitrogen (TN) and total phosphorus (TP) by BA-PIA were 66.8 ± 1.46% and 96.0 ± 0.98%, respectively. In addition, capping of BA-PIA promotes the conversion of easily released nitrogen (free nitrogen) in the sediment to stable nitrogen (acid-hydrolyzable nitrogen). The content of weakly adsorbed phosphorus and iron-adsorbed phosphorus in the sediment was reduced. The relative abundance of nitrifying bacteria, denitrifying bacteria, and microorganisms carrying phosphatase genes (such as Actinobacteria ) in the sediment increased by 109.78%. The capping of BA-PIA not only effectively removed the nitrogen and phosphorus in water but greatly reduced the risk of nitrogen and phosphorus release from sediment. BA-PIA was able to make up for the deficiency of the aluminum-based phosphorus-locking agent (Al-PIA) that only removes phosphorus, giving it improved application prospects.

● Combined proposals achieved higher disinfection efficiencies than singular ones. ● Cl 2 produced the most DBPs, combined proposals can reduce their formation. ● Cl 2 could damage bacterial cell membrane and caused the leakage of IOM. ● The toxicity by zebrafish embryo followed: Cl 2≈O 3/Cl 2 > O 3 > O 3/UV/Cl 2 > UV > UV/Cl 2. ● UV/Cl 2 was suggested to deal with COVID-19 epidemic for sewage treatment plants. During the COVID-19 pandemic, most sewage treatment plants increased disinfectant dosages to inactivate pathogenic viruses and microorganisms more effectively. However, this approach also led to the production of more disinfection by-products (DBPs). To ensure both disinfection efficiency and a reduction in DBP formation, new disinfection protocols are required. In this study, the disinfection efficiency, DBP amounts, and toxicity changes resulting from ozone (O 3), ultraviolet (UV), chlorine (Cl 2), and their combined processes were examined. The results demonstrated that the O 3/UV/Cl 2 combination achieved the highest disinfection efficiency. Chlorination produced the most DBPs, whereas UV treatment reduced the formation of trihalomethane (THM), halogenated ketones (HKs), haloacetic acids (HAA), dichloroacetonitrile (DCAN) and N-nitrosodimethylamine (NDMA) by 45.9%, 52.6%, 82.0%, 67.95%, and 47%, respectively. O 3 also significantly reduced their production by 99.1%, 91.1%, 99.5%, 100%, and 35%. Intracellular organic matter (IOM) was identified as the primary DBP precursors, producing 2.94 times more DBPs than extracellular organic matter (EOM). The increased DBP formation during chlorination was attributed to IOM leakage and cell membrane damage, which was verified using scanning electron microscopy (SEM). The toxicities of DBPs were evaluated for six disinfection methods, revealing inconsistent results. The overall toxicities were assessed using zebrafish embryo experiments. Both evaluations indicated that chlorination alone was the least favorable method. In addition, the toxicities followed a sequence: Cl 2 ≈ O 3/Cl 2 > O 3 > O 3/UV/Cl 2 > UV > UV/Cl 2. These findings can serve as a reference for sewage treatment plants in selecting appropriate disinfection methods to manage the COVID-19 epidemic from comprehensive perspective.