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This paper looks at 3D model retrieval techniques for digital virtual workshops in cloud computing. The global D2 shape distribution features are first used to sort the models for initial retrieval. Then the local curvature features are used to further optimize the sorted results to obtain the final retrieval results. Through the historical and cultural theme scene design, stage performance scene design, and interactive performance design to enhance the immersive experience of the corresponding cultural creations, the creative inheritance and dissemination mode of Dabie Mountain culture in the new period is created. The results show that the difference in the percentage of attention points in the landscape node area is significant, P=0.032, indicating that the virtual reality experience group is better able to meet the needs of the experience. The weights of the three evaluation dimensions of the virtual experience of Dabie Mountain folk song culture are, in order, mood design (0.4073) > scene design (0.3814) > context design (0.2113). Designing multi-sensory (visual, auditory, olfactory, tactile) is of the highest importance. In terms of interactive experience, more attention is paid to the timeliness and accuracy of feedback, as well as the guidance of operations. In terms of emotional experience, more attention is paid to the sense of pleasure, surprise, and satisfaction. In the cultural experience, more attention is paid to traditional folk songs and historical architectural displays.

Finite time control and finite time function projective synchronization for complex networks are studied in this paper. The complex networks with time-varying delay and function projective synchronization are achieved in finite time. The method of pinning control is used to achieve the finite time projective synchronization. A few target nodes are chosen to finish pinning function projective synchronization in a finite time by construct a time-dependent Lyapunov function. An example is provided to illustrate the correctness of the theoretical analysis.

s Background Long-stranded non-coding RNA TUG1 is lowly expressed in osteoarthritic chondrocytes. This study aimed to elucidate the role of TUG1 in osteoarthritic cartilage damage and the underlying mechanisms. Methods Combined database analysis, using primary chondrocytes as well as the C28/I2 cell line, was performed by qRT-PCR, Western blotting, and immunofluorescence to determine the expression of TUG1, miR-144-3p, DUSP1, and other target proteins. Dual luciferase reporter gene and RIP to verify direct interaction of TUG1 with miR-144–3-p and miR-144–3-p with DUSP1, Annexin V-FITC/PI double staining to detect apoptosis. CCK-8 to detect cell proliferation. The biological significance of TUG1, miR-144-3p, and DUSP1 was assessed in vitro experiments using siRNA for TUG1, mimic and repressor for miR-144-3p, and overexpression plasmid for DUSP1. In this study, all data were subjected to a t -test or one-way analysis of variance with a p -value < 0.05 as the cutoff. Results TUG1 expression was closely associated with osteoarthritic chondrocyte damage, and knockdown of TUG1 significantly promoted chondrocyte apoptosis and inflammation. In the present study, we found that TUG1 inhibited chondrocyte apoptosis and inflammation by competitively binding miR-144-3p, deregulating the negative regulatory effect of miR-144-3p on DUSP1, promoting DUSP1 expression, and inhibiting the p38 MAPK signaling pathway. Conclusions In conclusion, our study clarifies the role of the ceRNA regulatory network of TUG1/miR-144-3p/DUSP1/P38 MAPK in OA cartilage injury and provides an experimental and theoretical basis for genetic engineering tools to promote articular cartilage repair. Graphical abstract

This study (ORIENT-4) aimed to assess the efficacy and safety of sintilimab, a humanized anti-PD-1 antibody, in patients with relapsed/refractory extranodal NK/T cell lymphoma (r/r ENKTL). ORIENT-4 is a multicenter, single-arm, phase 2 clinical trial (NCT03228836). Patients with r/r ENKTL who failed to at least one asparaginase-based regimen were enrolled to receive sintilimab 200 mg intravenously every 3 weeks for up to 24 months. The primary endpoint was the objective response rate (ORR) based on Lugano 2014 criteria. Twenty-eight patients with r/r ENKTL were enrolled from August 31, 2017 to February 7, 2018. Twenty-one patients (75.0%, 95% CI: 55.1–89.3%) achieved an objective response. With a median follow-up of 30.4 months, the median overall survival (OS) was not reached. The 24-month OS rate was 78.6% (95% CI, 58.4–89.8%). Most treatment-related adverse events (TRAEs) were grade 1–2 (71.4%), and the most common TRAE was decreased lymphocyte count (42.9%). Serious adverse events (SAEs) occurred in 7 (25.0%) patients, and no patient died of adverse events. Sintilimab is effective and well tolerated in patients with r/r ENKTL and could be a novel therapeutic approach for the control of ENKTL in patients.

With the development of underground engineering and oil and gas exploitation, the problem of fluid-solid interaction in fractured rock mass has become increasingly prominent, and its complex coupling mechanism has a profound impact on the stability and safety of engineering. The coupling effect between fluid and solid skeleton in fractured rock mass under the interaction of stress field and seepage field is discussed in this study. Based on the effective stress principle, the effective stress formula including porosity is constructed, and the governing formula of elastic fluid-solid coupling stress field considering the deformation of solid skeleton is developed. By introducing non-Darcy seepage formula and continuity formula, the control formula of seepage field is derived. The results show that the saddle-junction bifurcation occurs when the boundary stress does not change with time. When the boundary stress changes with time, it has a significant effect on the coupled seepage system. In the long time scale, the system reaches the stable state slowly, the volume strain changes slowly, and the boundary stress is obviously affected. This study provides a theoretical basis for understanding the interaction between fluid flow and solid deformation in fractured rock mass.

We generated a library of ~1000 Drosophila stocks in which we inserted a construct in the intron of genes allowing expression of GAL4 under control of endogenous promoters while arresting transcription with a polyadenylation signal 3’ of the GAL4. This allows numerous applications. First, ~90% of insertions in essential genes cause a severe loss-of-function phenotype, an effective way to mutagenize genes. Interestingly, 12/14 chromosomes engineered through CRISPR do not carry second-site lethal mutations. Second, 26/36 (70%) of lethal insertions tested are rescued with a single UAS-cDNA construct. Third, loss-of-function phenotypes associated with many GAL4 insertions can be reverted by excision with UAS-flippase. Fourth, GAL4 driven UAS-GFP/RFP reports tissue and cell-type specificity of gene expression with high sensitivity. We report the expression of hundreds of genes not previously reported. Finally, inserted cassettes can be replaced with GFP or any DNA. These stocks comprise a powerful resource for assessing gene function. Determining what role newly discovered genes play in the body is an important part of genetics. This task requires a lot of extra information about each gene, such as the specific cells where the gene is active, or what happens when the gene is deleted. To answer these questions, researchers need tools and methods to manipulate genes within a living organism. The fruit fly Drosophila is useful for such experiments because a toolbox of genetic techniques is already available. Gene editing in fruit flies allows small pieces of genetic information to be removed from or added to anywhere in the animal’s DNA. Another tool, known as GAL4-UAS, is a two-part system used to study gene activity. The GAL4 component is a protein that switches on genes. GAL4 alone does very little in Drosophila cells because it only recognizes a DNA sequence called UAS. However, if a GAL4-producing cell is also engineered to contain a UAS-controlled gene, GAL4 will switch the gene on. Lee et al. used gene editing to insert a small piece of DNA, containing the GAL4 sequence followed by a ‘stop’ signal, into many different fly genes. The insertion made the cells where each gene was normally active produce GAL4, but – thanks to the stop signal – rendered the rest of the original gene non-functional. This effectively deleted the proteins encoded by each gene, giving information about the biological processes they normally control. Lee et al. went on to use their insertion approach to make a Drosophila genetic library. This is a collection of around 1,000 different strains of fly, each carrying the GAL4/stop combination in a single gene. The library allows any gene in the collection to be studied in detail simply by combining the GAL4 with different UAS-controlled genetic tools. For example, introducing a UAS-controlled marker would pinpoint where in the body the original gene was active. Alternatively, adding UAS-controlled human versions of the gene would create humanized flies, which are a valuable tool to study potential disease-causing genes in humans. This Drosophila library is a resource that contributes new experimental tools to fly genetics. Insights gained from flies can also be applied to more complex animals like humans, especially since around 65% of genes are similar across humans and Drosophila. As such, Lee et al. hope that this resource will help other researchers shed new light on the role of many different genes in health and disease.

The phytochemical investigation of the roots of the traditional Chinese medicinal plant Sophora flavescens led to the isolation of two novel prenylflavonoids with an unusual cyclohexyl substituent instead of the common aromatic ring B, named 4′,4′-dimethoxy-sophvein (17) and sophvein-4′-one (18), and 34 known compounds (1–16, 19–36). The structures of these chemical compounds were determined by spectroscopic techniques, including 1D-, 2D-NMR, and HRESIMS data. Furthermore, evaluations of nitric oxide (NO) production inhibitory activity against lipopolysaccharide (LPS)-treated RAW264.7 cells indicated that some compounds exhibited obvious inhibition effects, with IC50 ranged from 4.6 ± 1.1 to 14.4 ± 0.4 μM. Moreover, additional research demonstrated that some compounds inhibited the growth of HepG2 cells, with an IC50 ranging from 0.46 ± 0.1 to 48.6 ± 0.8 μM. These results suggest that flavonoid derivatives from the roots of S. flavescens can be used as a latent source of antiproliferative or anti-inflammatory agents.

The stacking between nanosheets is an intriguing and inevitable phenomenon in the chemistry of nano-interfaces. Two-dimensional metal-organic framework nanosheets are an emerging type of nanosheets with ultrathin and porous features, which have high potential in separation applications. Here, the stacking between single-layer metal-organic framework nanosheets is revealed to show three representative conformations with tilted angles of 8°, 14°, and 30° for Zr-1, 3, 5-(4-carboxylphenyl)-benzene framework as an example. Efficient untwisted stacking strategy by simple heating is proposed. A detailed structural analysis of stacking modes reveals the creation of highly ordered sub-nanometer micropores in the interspacing of untwisted nano-layers, yielding a high-resolution separator for the pair of para -/ meta -isomers over the twisted counterparts and commercial HP-5MS and VF-WAXMS columns. This general method is proven by additional nanosheet examples and supported by Grand Canonical Monte Carlo simulation. This finding will provide a synthetic route in the rational design of functionalities in two-dimensional metal-organic framework nanosheet. Metal-organic framework nanosheets are promising for separations, but interactions among them, affecting the performance, are largely unexplored. The authors reveal the favored stacking modes in a model system, and that untwisted restacking by thermal treatment improves isomer separation performance in gas chromatography capillary columns.

Current Deep Learning (DL)-based methods for vertebra localization in biplanar X-ray images mainly focus on two-dimensional (2D) information and neglect the projective geometry, limiting the accuracy of 3D navigation in X-ray-guided spine surgery. A 3D vertebra localization method from calibrated biplanar X-ray images is highly desired to address the problem. In this study, a projective-geometry-aware network for localizing 3D vertebrae in calibrated biplanar X-ray images, referred to as ProVLNet, is proposed. The network design of ProVLNet features three components: a Siamese 2D feature extractor to extract local appearance features from the biplanar X-ray images, a spatial alignment fusion module to incorporate the projective geometry in fusing the extracted 2D features in 3D space, and a 3D landmark regression module to regress the 3D coordinates of the vertebrae from the 3D fused features. Evaluated on two typical and challenging datasets acquired from the lumbar and the thoracic spine, ProVLNet achieved an identification rate of 99.53% and 98.98% and a point-to-point error of 0.64 mm and 1.38 mm, demonstrating superior performance of our proposed approach over the state-of-the-art (SOTA) methods.

Gliomas, originating from glial cells, are prevalent and aggressive brain tumors with high recurrence rates and poor prognosis. Despite advancements in surgical, radiation, and chemotherapeutic treatments, the survival rates remain low. Current standard therapies, such as Temozolomide, have limitations due to cytotoxicity, restricted effectiveness, and severe side effects. So, the development of safer anti-glioma agents is the need of the hour. Bioactive compounds of plant origin, either natural or synthetic, have potential implications due to them actively attacking different targets with a wide range of bioactivities, including anti-glioma activities. In this review, for the first time, there is an overall overview of 51 small molecules of plant origin and seven of their synthetic derivatives, represented as anti-glioma agents in the past decades. The goal of the present review is to provide a summary to comprehend the anti-glioma effects of these compounds in addition to providing a reference for preclinical research into novel anti-glioma agents for future clinical application.