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The oceans and all the marine life forms that they harbor remain a major source of treasure to mankind, covering 71% of the earth's surface and representing over 95% of the biosphere. Zoanthids, a subclass of the benthic Anthozoans, are found in almost all marine environments. Despite this fact, the order Zoantharia is still one of the most taxonomically neglected and least examined orders of the phylum Cnidaria. However, they are gaining importance in the pharmaceutical research field due to the significance of bioactive compounds secreted from their bodies. Zoanthus sansibaricus species collected from the Indian coast have been explored in the present study. The study aimed to isolate chemical constituents from the methanol and methanol: chloroform (1:1) extract of Zoanthus sp. by subjecting it to a series of chromatographic analysis. In total, eight compounds were purified from petroleum ether, chloroform, n-butanol, and methanol-soluble parts of aqueous fractions. The compounds were characterized using spectroscopic techniques such as Ultraviolet-Visible (UV-Vis), Fourier Transform Infrared (FTIR), 1H and 13C Nuclear Magnetic Resonance (NMR), and Mass spectrometry. Total phenolic content and total flavonoid content were estimated using the Folin-Ciocalteu method and Aluminum chloride method, respectively. The phenolic compound contents of the petroleum ether and methanol-soluble part of the aqueous fraction were 18.92mg/g GAE and 7.02mg/g GAE, respectively. Total flavonoid content in the petroleum ether fraction was 8.05mg/g QUE and 1.38mg/g QUE in the methanol-soluble part of the aqueous fraction. This study emphasizes the need for a more extensive compilation of such data to extend our pool of knowledge about marine natural products as potential candidates for drug discovery.

Lysinibacillus sphaericus D3 cell-immobilized beads in natural gel sodium alginate decolorized the xylidine orange dye 1-(dimethylphenylazo)-2-naphthol-6-sulfonic acid sodium salt in the laboratory. Optimal conditions were selected for decolorization and the products formed were evaluated for toxicity by disc diffusion assay against common marine bacteria which revealed the non-toxic nature of the dye-degraded products. Decolorization of the brightly colored dye to colorless products was measured on an Ultra Violet-Vis spectrophotometer and its biodegradation products monitored on Thin Layer Chromatographic plate and High Performance Liquid Chromatography (HPLC). Finally, the metabolites formed in the decolorized medium were characterized by mass spectrometry. This analysis confirms the conversion of the parent molecule into lower molecular weight aromatic phenols and sulfonic acids as the final products of biotransformation. Based on the results, the probable degradation products of xylidine orange were naphthol, naphthylamine-6-sulfonic acid, 2-6-dihydroxynaphthalene, and bis-dinaphthylether. Thus, it may be concluded that the degradation pathway of the dye involved (a) reduction of its azo group by azoreductase enzyme (b) dimerization of the hydrazo compound followed by (c) degradation of monohydrazo as well as dimeric metabolites into low molecular weight aromatics. Finally, it may be worth exploring the possibility of commercially utilizing L. sphaericus D3 for industrial applications for treating large-scale dye waste water.

Marine organisms and their metabolites represent a unique source of potential pharmaceutical substances. In this study, we examined marine-derived substances for their bioactive properties in a cell-based Chikungunya virus (CHIKV) replicon model and for in vitro anti-inflammatory activity. In the screening of a marine sample library, crude extracts from the Indian soft coral, Sinularia kavarattiensis, showed promising activity against the CHIKV replicon. Bioassay-guided chemical fractionation of S. kavarattiensis resulted in the isolation of six known norcembranoids (1–6) and one new compound, named kavaranolide (7). The structures were elucidated on the basis of NMR and MS spectroscopic data. Compounds 1–3 and 5–7 were evaluated for their replicon-inhibiting potential in the CHIKV model by using a luminescence-based detection technique and live cell imaging. Compounds 1 and 2 showed moderate inhibition of the CHIKV replicon, but imaging studies also revealed cytotoxic properties. Moreover, the effects of the isolated compounds on primary microglial cells, an experimental model for neuroinflammation, were evaluated. Compound 2 was shown to modulate the immune response in microglial cells and to possess potential anti-inflammatory properties by dose-dependently reducing the release of pro- and anti-inflammatory cytokines.

Allelochemicals are secondary metabolites which function as a natural protection against grazing activities by algae and higher plants. They are one of the major metabolites engaged in the interactions of organisms. The chemically mediated interactions between organisms significantly influence the functioning of the ecosystems. Most of these compounds are secondary metabolites comprising sterols, terpenes, and polyphenols. These compounds not only play a defensive role, but also exhibit biological activities such as antioxidants, anti-cancer, anti-diabetes, anti-inflammation, and anti-microbial properties. This review article discusses the current understanding of the allelochemicals of seaweeds and their bioprospecting potential that can bring benefit to humanity. Specifically, the bioactive substances having specific health benefits associated with the consumption or application of seaweed-derived compounds. The properties of such allelochemicals can have implications for bioprospecting pharmaceutical, nutraceutical and cosmetic applications.

The extraction of Rhizostoma pulmo discarded off the Goa coast, India resulted in obtaining remarkably higher yield of 47% (based on lyophilized weight) type I collagen. The amino acid composition showed presence of glycine, hydroxyproline and proline and other amino acids in consistent with mammalian collagens. Interestingly, it also possessed essential amino acid tryptophan that makes this collagen superior than other commercially available collagen products. The dose and time response anti-oxidant activity (DPPH assay) of jellyfish collagen showed increase in the percentage activity with its efficiency better than marine fish collagen. In the dose response, IC50 was found to be 11.0 mg/ml. The percentage DPPH activity gradually increased from 47.58 to 81.11% with time 1–8 h, respectively at concentration of 7 mg/ml of jellyfish collagen. It was noteworthy to observe that the anti-oxidant activity remained 80% even after 24 h of analysis. The EDX analysis showed presence of minerals like Cl, Na, Mg, K, Cu, Fe, Zn etc. essential for healthy bones. The mass assisted laser desorption ionization-time of flight mass spectrometric (MALDI-TOF MS) data showed several precursor peaks of different peptides which has been presented here for the first time. The finding showed higher production of tryptophan containing anti-oxidant collagen that will certainly enhance its benefit in neurotransmission and cognitive function.