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"Torres, R."
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Polyethylene glycol improves current methods for circulating extracellular vesicle-derived DNA isolation
Background
Extracellular vesicles (EVs) are small membrane-bound vesicles which play an important role in cell-to-cell communication. Their molecular cargo analysis is presented as a new source for biomarker detection, and it might provide an alternative to traditional solid biopsies. However, the most effective approach for EV isolation is not yet well established.
Results
Here, we study the efficiency of the most common EV isolation methods-ultracentrifugation, Polyethlyene glycol and two commercial kits, Exoquick
®
and PureExo
®
. We isolated circulating EVs from the bloodstream of healthy donors, characterized the size and yield of EVs and analyzed their protein profiles and concentration. Moreover, we have used for the first time Digital-PCR to identify and detect specific gDNA sequences, which has several implications for diagnostic and monitoring many types of diseases.
Conclusions
Our findings present Polyethylene glycol precipitation as the most feasible and less cost-consuming EV isolation technique.
Journal Article
Non-singular quantum improved rotating black holes and their maximal extension
We add a prescription to the Newman–Janis algorithm in order to use it as a means of finding new extended rotating black hole spacetimes from static spherically symmetric ones. Then, we apply the procedure to a quantum improved black hole spacetime coming from Quantum Einstein Gravity in order to get the maximally extended spacetime corresponding to a non-singular rotating black hole. We rigourously check for the existence of scalar curvature singularities in the quantum improved rotating spacetime and we show that it is devoid of them. We also analyze the horizons and causal structure of the rotating black hole and provide Penrose diagrams for the maximally extended spacetime.
Journal Article
Selective genome editing of amplified oncogenes triggers immunogenic cell death and tumor remodeling
Oncogene amplifications fuel some of the most lethal, therapy‑refractory cancers, yet remain clinically untargeted. We report a single‑guide CRISPR/Cas9 strategy that converts the sheer copy‑number excess of oncogene amplicons into an Achilles’ heel. A solitary intronic double‑strand break is innocuous in diploid genomes but collapses oncogene amplification‑positive cells across neuroblastoma, small‑cell lung and colorectal carcinoma models, driving > 90% loss of viability, G₂/M blockade and catastrophic DNA‑damage signalling. Amplified‑locus cleavage rewires transcription toward cell death activation, necroptosis and cGAS–STING–mediated immunogenic cell death, enabling dendritic‑cell cross‑priming and T‑cell activation and proliferation. In xenografts, delivery of the intronic sgRNA shrinks tumours by 90%, prolongs survival and remodels the innate tumour microenvironment. Deep sequencing confirms negligible off‑target editing, and combination with doxorubicin achieves supra‑additive killing. These findings establish amplification density, not sequence content, as a tractable, tumour‑exclusive target and unveil a dual‑action platform that is simultaneously cytotoxic and immunostimulatory. Editing of tumor amplifications therefore offers a blueprint for translating copy‑number aberrations into precision genome‑editing therapies for treatment‑resistant cancers.
Journal Article
Brake : seconds to live : a nation to save
Jeremy Reins wakes up in a cramped space with the only light coming from the digital numbers ticking away above his head. Confused and disoriented with no one answering his cries for help, he suddenly hears an engine rev and his predicament becomes clear: he's trapped in the trunk of a moving car. As his captors reveal themselves, Jeremy realizes he won't be set free until he gives up the whereabouts of a secret location where the U.S. President is taken in the event of a terrorist attack.
DVD
Many-body factorization and position–momentum equivalence of nuclear short-range correlations
While mean-field approximations, such as the nuclear shell model, provide a good description of many bulk nuclear properties, they fail to capture the important effects of nucleon–nucleon correlations such as the short-distance and high-momentum components of the nuclear many-body wave function1. Here, we study these components using the effective pair-based generalized contact formalism2,3 and ab initio quantum Monte Carlo calculations of nuclei from deuteron to 40Ca (refs. 4–6). We observe a universal factorization of the many-body nuclear wave function at short distance into a strongly interacting pair and a weakly interacting residual system. The residual system distribution is consistent with that of an uncorrelated system, showing that short-distance correlation effects are predominantly embedded in two-body correlations. Spin- and isospin-dependent ‘nuclear contact terms’ are extracted in both coordinate and momentum space for different realistic nuclear potentials. The contact coefficient ratio between two different nuclei shows very little dependence on the nuclear interaction model. These findings thus allow extending the application of mean-field approximations to short-range correlated pair formation by showing that the relative abundance of short-range pairs in the nucleus is a long-range (that is, mean field) quantity that is insensitive to the short-distance nature of the nuclear force.Effects of nucleon–nucleon correlations are studied with the generalized contact formalism and ab initio quantum Monte Carlo calculations. For nuclei from deuteron to 40Ca, the many-body nuclear wave function is shown to factorize at short distances.
Journal Article
On regular rotating black holes
Different proposals for regular rotating black hole spacetimes have appeared recently in the literature. However, a rigorous analysis and proof of the regularity of this kind of spacetimes is still lacking. In this note we analyze rotating Kerr-like black hole spacetimes and find the necessary and sufficient conditions for the regularity of all their second order scalar invariants polynomial in the Riemann tensor. We also show that the regularity is linked to a violation of the weak energy conditions around the core of the rotating black hole.
Journal Article
Augmented Reality-Assisted Ultrasound Breast Biopsy
Breast cancer is the most prevalent cancer in the world and the fifth-leading cause of cancer-related death. Treatment is effective in the early stages. Thus, a need to screen considerable portions of the population is crucial. When the screening procedure uncovers a suspect lesion, a biopsy is performed to assess its potential for malignancy. This procedure is usually performed using real-time Ultrasound (US) imaging. This work proposes a visualization system for US breast biopsy. It consists of an application running on AR glasses that interact with a computer application. The AR glasses track the position of QR codes mounted on an US probe and a biopsy needle. US images are shown in the user’s field of view with enhanced lesion visualization and needle trajectory. To validate the system, latency of the transmission of US images was evaluated. Usability assessment compared our proposed prototype with a traditional approach with different users. It showed that needle alignment was more precise, with 92.67 ± 2.32° in our prototype versus 89.99 ± 37.49° in a traditional system. The users also reached the lesion more accurately. Overall, the proposed solution presents promising results, and the use of AR glasses as a tracking and visualization device exhibited good performance.
Journal Article