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Gene flow between domesticated plants and their wild relatives is one of the major evolutionary processes acting to shape their structure of genetic diversity. Earlier literature, in the 1970s, reported on the interfertility and the sympatry of wild, weedy and cultivated sorghum belonging to the species Sorghum bicolor in most regions of sub-Saharan Africa. However, only a few recent surveys have addressed the geographical and ecological distribution of sorghum wild relatives and their genetic structure. These features are poorly documented, especially in western Africa, a centre of diversity for this crop. We report here on an exhaustive in situ collection of wild, weedy and cultivated sorghum assembled in Mali and in Guinea. The extent and pattern of genetic diversity were assessed with 15 SSRs within the cultivated pool (455 accessions), the wild pool (91 wild and weedy forms) and between them. F ST and R ST statistics, distance-based trees, Bayesian clustering methods, as well as isolation by distance models, were used to infer evolutionary relationships within the wild–weedy–crop complex. Firstly, our analyses highlighted a strong racial structure of genetic diversity within cultivated sorghum ( F ST  = 0.40). Secondly, clustering analyses highlighted the introgressed nature of most of the wild and weedy sorghum and grouped them into two eco-geographical groups. Such closeness between wild and crop sorghum could be the result of both sorghum’s domestication history and preferential post-domestication crop-to-wild gene flow enhanced by farmers’ practices. Finally, isolation by distance analyses showed strong spatial genetic structure within each pool, due to spatially limited dispersal, and suggested consequent gene flow between the wild and the crop pools, also supported by R ST analyses. Our findings thus revealed important features for the collection, conservation and biosafety of domesticated and wild sorghum in their centre of diversity.

Smallholder farmers in Northern Ghana face challenges due to weather variability and market volatility, hindering their ability to invest in sustainable intensification options. Modeling can help understand the relationships between productivity, environmental, and economical aspects, but few models have explored the effects of weather variability on crop management and resource allocation. This study introduces an integrated modeling approach to optimize resource allocation for smallholder mixed crop and livestock farming systems in Northern Ghana. The model combines a process-based crop model, farm simulation model, and annual optimization model. Crop model simulations are driven by a large ensemble of weather time series for two scenarios: good and bad weather. The model accounts for the effects of climate risks on farm management decisions, which can help in supporting investments in sustainable intensification practices, thereby bringing smallholder farmers out of poverty traps. The model was simulated for three different farm types represented in the region. The results suggest that farmers could increase their income by allocating more than 80% of their land to cash crops such as rice, groundnut, and soybeans. The optimized cropping patterns have an over 50% probability of increasing farm income, particularly under bad weather scenarios, compared with current cropping systems.

The production of soybean is gaining more attention in West Africa. In light of projected changes in climate, there is a need to assess the potential impacts on yield productivity and variability among farmers. An evaluated GROPGRO module of the Decision Support System for Agro-technological Transfer (DSSAT) was used to simulate soybean productivity under both historical (1980–2009) and projected climate scenarios from multiple general circulation models (GCMs) under two representative concentration pathways (RCPs): 4.5 and 8.5. Agronomic data from 90 farms, as well as multiple soil profile data, were also used for the impact assessment. Climate change leads to a reduction (3% to 13.5% across GCMs and RCPs) in the productivity of soybean in Northern Ghana. However, elevated atmospheric carbon dioxide has the potential to offset the negative impact, resulting in increased (14.8% to 31.3% across GCMs and RCPs) productivity. The impact of climate change on yield varied widely amongst farms (with relative standard deviation (RSD) ranging between 17% and 35%) and across years (RSD of between 10% and 15%). Diversity in management practices, as well as differences in soils, explained the heterogeneity in impact among farms. Variability among farms was higher than that among years. The strategic management of cultural practices provides an option to enhance the resilience of soybean productivity among smallholders

Soil fertility in smallholder farming areas is known to vary strongly on multiple scales. This study measures the sensitivity of the recorded satellite signal to on-farm soil fertility treatments applied to five crop types, and quantifies this fertilization effect with respect to within-field variation, between-field variation and field position in the catena. Plant growth was assessed in 5–6 plots per field in 48 fields located in the Sudano-Sahelian agro-ecological zone of southeastern Mali. A unique series of Very High Resolution (VHR) satellite and Unmanned Aerial Vehicle (UAV) images were used to calculate the Normalized Difference Vegetation Index (NDVI). In this experiment, for half of the fields at least 50% of the NDVI variance within a field was due to fertilization. Moreover, the sensitivity of NDVI to fertilizer application was crop-dependent and varied through the season, with optima at the end of August for peanut and cotton and early October for sorghum and maize. The influence of fertilizer on NDVI was comparatively small at the landscape scale (up to 35% of total variation), relative to the influence of other components of variation such as field management and catena position. The NDVI response could only partially be benchmarked against a fertilization reference within the field. We conclude that comparisons of the spatial and temporal responses of NDVI, with respect to fertilization and crop management, requires a stratification of soil catena-related crop growth conditions at the landscape scale.

Historically the western sahelian dry regions of Mali are known to be highly endemic for cutaneous leishmaniasis (CL) caused by Leishmania major, while cases are rarely reported from the Southern savanna forest of the country. Here, we report baseline prevalence of CL infection in 3 ecologically distinct districts of Mali (dry sahelian, north savanna and southern savanna forest areas). We screened 195 to 250 subjects from 50 to 60 randomly selected households in each of the 6 villages (four from the western sahelian district of Diema in Kayes region, one from the central district of Kolokani and one from the southern savanna district of Kolodieba, region of Sikasso). The screening consisted of: 1] A Leishmanin Skin Test (LST) for detection of exposure to Leishmania parasites; 2] clinical examination of suspected lesions, followed by validation with PCR and 3] finger prick blood sample to determine antibody levels to sand fly saliva. LST positivity was higher in the western district of Diema (49.9%) than in Kolokani (24.9%) and was much lower in Kolondieba (2.6%). LST positivity increased with age rising from 13.8% to 88% in Diema for age groups 2-5 years and 41-65 years, respectively. All eight PCR-confirmed L. major CL cases were diagnosed in subjects below 18 years of age and all were residents of the district of Diema. Exposure to sand fly bites, measured by anti-saliva antibody titers, was comparable in individuals living in all three districts. However, antibody titers were significantly higher in LST positive individuals (P<0.0001). In conclusion, CL transmission remains active in the western region of Mali where lesions were mainly prevalent among children under 18 years old. LST positivity correlated to higher levels of antibodies to sand fly salivary proteins, suggesting their potential as a risk marker for CL acquisition in Mali.

[This corrects the article DOI: 10.1371/journal.pntd.0005141.].

Agriculture in West Africa is constrained by several yield-limiting factors, such as poor soil fertility, erratic rainfall distributions and low input systems. Projected changes in climate, thus, pose a threat since crop production is mainly rain-fed. The impact of climate change and its variation on the productivity of cereals in smallholder settings under future production systems in Navrongo, Ghana and Nioro du Rip, Senegal was assessed in this study. Data on management practices obtained from household surveys and projected agricultural development pathways (through stakeholder engagements), soil data, weather data (historical: 1980–2009 and five General Circulation Models; mid-century time slice 2040–2069 for two Representative Concentration Pathways; 4.5 and 8.5) were used for the impact assessment, employing a crop simulation model. Ensemble maize yield changes under the sustainable agricultural development pathway (SDP) were −13 and −16%, while under the unsustainable development pathway (USDP), yield changes were −19 and −20% in Navrongo and Nioro du Rip, respectively. The impact on sorghum and millet were lower than that on maize. Variations in climate change impact among smallholders were high with relative standard deviations (RSD) of between 14% and 60% across the cereals with variability being higher under the USDP, except for millet. Agricultural production systems with higher intensification but with less emphasis on soil conservation (USDP) will be more negatively impacted by climate change compared to relatively sustainable ones (SDP).

The potential gene flow between a crop and its wild relatives is largely determined by the overlaps in their ecological and geographical distributions. Ecogeographical databases are therefore indispensable tools for the sustainable management of genetic resources. In order to expand our knowledge of Sorghum bicolor distribution in Kenya, we conducted in situ collections of wild, weedy and cultivated sorghum. Qualitative and quantitative morphological traits were measured for each sampled wild sorghum plant. Farmers’ knowledge relating to the management of sorghum varieties and autecology of wild sorghum was also obtained. Cluster analysis supports the existence of several wild sorghum morphotypes that might correspond to at least three of the five ecotypes recognized in Africa. Intermediate forms between wild and cultivated sorghum belonging to the S. bicolor ssp. drummondii are frequently found in predominantly sorghum growing areas. Crop-wild gene flow in sorghum is likely to occur in many agroecosystems of Kenya.

Background Novel treatments against for tinea capitis are needed, and the natural aminosterol squalamine is a potential topical antidermatophyte drug candidate. Objectives This phase II randomized double-blind placebo-controlled clinical trial aimed at testing the efficacy and safety of a three-week squalamine ointment regimen for the treatment of tinea capitis . Patients Males aged 6–15 years presenting with tinea capitis were treated with either topical squalamine ointment or placebo for 3 weeks. The primary endpoint was complete clinical cure. The secondary endpoints were the occurrence of local and/or systemic adverse events, mycological cure, and partial clinical response. Prospective follow-up of clinical adverse events was performed daily. Results Five patients were treated with 1 % squalamine ointment and 15 with placebo. No complete cure was observed. No clinical or biological adverse event was recorded. A significantly ( p  = 0.03) better hair-growth score, indicating a partial clinical improvement of the tinea capitis lesion, was observed in the patients treated with squalamine compared to those treated with placebo. Conclusion This three-week squalamine ointment regimen was well tolerated and showed an encouraging partial clinical activity for the treatment of tinea capitis . Further studies are needed to evaluate the efficacy of topical squalamine alone against tinea corporis or in combination with a systemic antidermatophyte drug against tinea capitis .

Mali is currently experiencing a polycrisis, characterised by the interplay of growing socio-political insecurity, massive population displacements and recurrent tensions in the functioning of the health system and the provision of care. In this context, the multidisciplinary teams of University Community Health Centres (CSCoM-Us), primary health care facilities, have expressed the desire to strengthen their skills to better meet the needs of the internally displaced persons who frequent their facilities. To address this demand, training workshops were co-constructed by a team of family physicians (FPs), combining the experiential knowledge of local teams with the expertise of partners from the Université de Sherbrooke. A Training of Trainer (ToT) programme, consisting of training provided by central-level trainers to local-level practitioners, was developed and deployed. Five priorities were identified by local partners: continuity of care, maternal health, gender-based violence, mental health and working with a language barrier. From 2022 to 2023, this training was implemented in Mali’s seven CSCOM-Us, reaching 277 health professionals in five regions of the country. The outcomes include increased awareness of the challenges faced by internally displaced persons and strengthening local capabilities. This short report highlights the strategic role and leadership played by FP in improving the population’s health, particularly in sub-Saharan Africa, through their versatility and community-oriented, holistic and patient-centred approach.