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7 result(s) for "Tsitouras, Alexandros"
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UAV Remote Sensing for Drought-Adaptive Sesame Breeding: Flight-Altitude Benchmarking, Predictive Modelling, and Composite Stress Tolerance Indexing
Early-generation sesame (Sesamum indicum L.) breeding requires high-throughput phenotyping of large unreplicated populations across contrasting environments. A DJI Phantom 4 Multispectral UAV was flown at 40, 80, and 120 m above ground level (AGL) over 588 M2 genotypes under full irrigation (ENV1) and terminal drought (ENV2; irrigation withheld from reproductive onset) on four dates (July–September 2025). Structure-from-motion canopy height models were compared with ground measurements, and four spectral reflectance indices—Normalised Difference Vegetation Index (NDVI), Normalised Difference Red Edge (NDRE), Green Normalised Difference Vegetation Index (GNDVI), and Leaf Chlorophyll Index (LCI)—were derived from 40 m imagery. Ordinary least squares (OLS), Random Forest, and Gradient Boosting were evaluated under leave-one-genotype-out (LOGO), leave-one-environment-out (LOEO), and leave-one-date-out (LODO) cross-validation; genotypic repeatability was quantified by intraclass correlation (ICC), and drought performance was ranked by a composite Stress Tolerance Index (STI) validated against an independent breeder assessment. The 40 m altitude gave the highest height accuracy (R2 = 0.812 in ENV1; 0.663 in ENV2). LOGO accuracy (R2 ≈ 0.83) fell to R2 ≈ 0.55 under LODO—the operationally relevant figure for a new phenological stage—and the full structural–spectral OLS model collapsed (R2 = −0.203) where tree ensembles remained stable. Spectral-index repeatability was up to ~2-fold higher under stress (ICC(3,4) > 0.84). The composite STI flagged 38 elite genotypes (7.6% of 498); 10 of its top 30 were confirmed in the breeder’s 48-best selection from all 588 rows—a 4.1-fold enrichment over chance (hypergeometric p = 4.5 × 10−5).
Variable-Rate Nitrogen Application in Wheat Based on UAV-Derived Fertilizer Maps and Precision Agriculture Technologies
Variable-rate nitrogen (VR-N) application allows farmers to optimize nitrogen (N) input site-specifically within field boundaries, enhancing both economic efficiency and environmental sustainability. In this study, VR-N technology was applied to durum wheat in two small-scale commercial fields (3–4 ha each) located in distinct agro-climatic zones of Thessaly, central Greece. A real-time VR-N application algorithm was used to calculate N rates based on easily obtainable near-real-time data from unmanned aerial vehicle (UAV) imagery, tailored to the crop’s actual needs. VR-N implementation was carried out using conventional fertilizer spreaders equipped to read prescription maps. Results showed that VR-N reduced N input by up to 49.6% compared to the conventional uniform-rate N (UR-N) application, with no significant impact on wheat yield or grain quality. In one of the fields, the improved gain of VR-N when compared to UR-N was 7.2%, corresponding to an economic gain of EUR 163.8 ha−1, while in the second field—where growing conditions were less favorable—no considerable VR-N economic gain was observed. Environmental benefits were also notable. The carbon footprint (CF) of the wheat crop was reduced by 6.4% to 22.0%, and residual soil nitrate (NO3−) levels at harvest were 13.6% to 36.1% lower in VR-N zones compared to UR-N zones. These findings suggest a decreased risk of NO3− leaching and ground water contamination. Overall, the study supports the viability of VR-N as a practical and scalable approach to improve N use efficiency (NUE) and reduce the environmental impact of wheat cultivation which could be readily adopted by farmers.
Spectral Reflectance Indices as a High Throughput Selection Tool in a Sesame Breeding Scheme
On-farm genotype screening is at the core of every breeding scheme, but it comes with a high cost and often high degree of uncertainty. Phenomics is a new approach by plant breeders, who use optical sensors for accurate germplasm phenotyping, selection and enhancement of the genetic gain. The objectives of this study were to: (1) develop a high-throughput phenotyping workflow to estimate the Normalized Difference Vegetation Index (NDVI) and the Normalized Difference Red Edge index (NDRE) at the plot-level through an active crop canopy sensor; (2) test the ability of spectral reflectance indices (SRIs) to distinguish between sesame genotypes throughout the crop growth period; and (3) identify specific stages in the sesame growth cycle that contribute to phenotyping accuracy and functionality and evaluate the efficiency of SRIs as a selection tool. A diversity panel of 24 sesame genotypes was grown at normal and late planting dates in 2020 and 2021. To determine the SRIs the Crop Circle ACS-430 active crop canopy sensor was used from the beginning of the sesame reproductive stage to the end of the ripening stage. NDVI and NDRE reached about the same high accuracy in genotype phenotyping, even under dense biomass conditions where “saturation” problems were expected. NDVI produced higher broad-sense heritability (max 0.928) and NDRE higher phenotypic and genotypic correlation with the yield (max 0.593 and 0.748, respectively). NDRE had the highest relative efficiency (61%) as an indirect selection index to yield direct selection. Both SRIs had optimal results when the monitoring took place at the end of the reproductive stage and the beginning of the ripening stage. Thus, an active canopy sensor as this study demonstrated can assist breeders to differentiate and classify sesame genotypes.
Analyzing the Impact of Storm ‘Daniel’ and Subsequent Flooding on Thessaly’s Soil Chemistry through Causal Inference
Storm ‘Daniel’ caused the most severe flood phenomenon that Greece has ever experienced, with thousands of hectares of farmland submerged for days. This led to sediment deposition in the inundated areas, which significantly altered the chemical properties of the soil, as revealed by extensive soil sampling and laboratory analysis. The causal relationships between the soil chemical properties and sediment deposition were extracted using the DirectLiNGAM algorithm. The results of the causality analysis showed that the sediment deposition affected the CaCO3 concentration in the soil. Also, causal relationships were identified between CaCO3 and the available phosphorus (P-Olsen), as well as those between the sediment deposit depth and available manganese. The quantified relationships between the soil variables were then used to generate data using a Multiple Linear Perceptron (MLP) regressor for various levels of deposit depth (0, 5, 10, 15, 20, 25, and 30 cm). Then, linear regression equations were fitted across the different levels of deposit depth to determine the effect of the deposit depth on CaCO3, P, and Mn. The results revealed quadratic equations for CaCO3, P, and Mn as follows: 0.001XCaCO32 + 0.08XCaCO3 + 6.42, 0.004XP2 − 0.26XP + 12.29, and 0.003XMn2 − 0.08XMn + 22.47, respectively. The statistical analysis indicated that corn growing in soils with a sediment over 10 cm requires a 31.8% increase in the P rate to prevent yield decline. Additional notifications regarding cropping strategies in the near future are also discussed.
Water Footprint of Industrial Tomato Cultivations in the Pinios River Basin: Soil Properties Interactions
Industrial tomatoes are cultivated in about 4000 ha of the Pinios river basin (central Greece), providing significant income to the farmers. In this study, the water footprint (WF) of industrial tomatoes between planting and harvest was estimated in 24 different farms for three consecutive years. The selected farms were representative of the main agro-climatic zones and soil textural classes within the river basin. Green, blue and grey WF calculations were based on datasets of the experimental plots for each farm, including irrigation water volume, meteorological, soil, and crop yield data. The results showed that the WF of tomatoes ranged from 37 to 131 m3 water/ton tomatoes with an average of 61 m3/ton. The WF variation depended mainly on crop yield, local agro-climatic and soil conditions. The green, blue, and grey WF components averaged 13, 27 and 21 m3/ton, respectively. The results reveal the importance of WF in understanding how tomato production relates to the sustainable use of freshwater and pollution at local level.
A Taguchi Approach for the Synthesis Optimization of Metakaolin Based Geopolymers
There are several factors that affect geopolymerization, including the type and ratios of the starting materials as well as the curing conditions of the initial mixture. The effect of the synthesis parameters on the formation of inorganic polymers are usually examined by “changing one factor at a time”. In this study Taguchi experimental designing model was applied in order to study the synergetic effect of selected synthesis parameters on the compressive strength development of metakaolin based geopolymers. The experimental design involved the variation of three control factors in five levels. The selected factors and the corresponding level range were: i) the alkali to aluminum molar ratio in the starting mixture (0.5-1.5), ii) the kind of alkali ion (Na and/or K) and iii) the molar ratio of Si to alkali oxide in the activation solution (0-2.0). The compressive strength of geopolymers was measured and the final products were also examined by means of XRD, FTIR and SEM. As it is concluded, the optimal synthesis conditions for metakaolin geopolymers are R/Al=0.75, Na/(Na+K)=0.50 and [Si]/R2O=1.50, while the factor having the highest impact on the development of compressive strength is the [Si]/R2O ratio.
Mapping of Weed Management Methods in Orchards using Sentinel-2 and PlanetScope Data
Effective weed management is crucial for improving agricultural productivity, as weeds compete with crops for vital resources like nutrients and water. Accurate maps of weed management methods are essential for policymakers to assess farmer practices, evaluate impacts on vegetation health, biodiversity, and climate, as well as ensure compliance with policies and subsidies. However, monitoring weed management methods is challenging as they commonly rely on ground-based field surveys, which are often costly, time-consuming and subject to delays. In order to tackle this problem, we leverage earth observation data and Machine Learning (ML). Specifically, we developed separate ML models using Sentinel-2 and PlanetScope satellite time series data, respectively, to classify four distinct weed management methods (Mowing, Tillage, Chemical-spraying, and No practice) in orchards. The findings demonstrate the potential of ML-driven remote sensing to enhance the efficiency and accuracy of weed management mapping in orchards.