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Graphene is a unique 2D material that provides exceptional mechanical properties, ultra-high carrier mobility, and dynamically adjustable surface conductivity, making it an excellent choice for microwave devices. However, the dynamic manipulation of amplitude and phase in substrate integrated waveguides based on multilayered graphene has not been experimentally proven. In this paper, we propose a novel multilayered graphene based half mode SIW attenuator and phase shifter for the manipulation of amplitude and phase at microwave frequencies. The amplitude and phase are controlled by applying a bias voltage to graphene. The applied voltage is increased from 0 to 6.2 V, and the sheet resistance (Ω/□) of graphene can be tuned between 1302 and 60 Ω/□, depending on the gap size for deposition of graphene. The simulated and measured results of the proposed attenuator and phase shifter demonstrate the manipulation of microwave signals. The dynamic range achieved for amplitude is 13dB, and that of phase is 45 degrees. The proposed technique proves that the amplitude and phase of microwave signals can be tuned in SIW based devices without any localized surface mounted devices (SMD) or complex realization techniques.

Visible light communication (VLC) is a new paradigm that could revolutionise the future of wireless communication. In VLC, information is transmitted through modulating the visible light spectrum (400–700 nm) that is used for illumination. Analytical and experimental work has shown the potential of VLC to provide high-speed data communication with the added advantage of improved energy efficiency and communication security/privacy. VLC is still in the early phase of research. There are fewer review articles published on this topic mostly addressing the physical layer research. Unlike other reviews, this article gives a system prespective of VLC along with the survey on existing literature and potential challenges toward the implementation and integration of VLC.

Several environmental stresses, including biotic and abiotic factors, adversely affect the growth and development of crops, thereby lowering their yield. However, abiotic factors, e.g., drought, salinity, cold, heat, ultraviolet radiations (UVr), reactive oxygen species (ROS), trace metals (TM), and soil pH, are extremely destructive and decrease crop yield worldwide. It is expected that more than 50% of crop production losses are due to abiotic stresses. Moreover, these factors are responsible for physiological and biochemical changes in plants. The response of different plant species to such stresses is a complex phenomenon with individual features for several species. In addition, it has been shown that abiotic factors stimulate multi-gene responses by making modifications in the accumulation of the primary and secondary metabolites. Metabolomics is a promising way to interpret biotic and abiotic stress tolerance in plants. The study of metabolic profiling revealed different types of metabolites, e.g., amino acids, carbohydrates, phenols, polyamines, terpenes, etc, which are accumulated in plants. Among all, primary metabolites, such as amino acids, carbohydrates, lipids polyamines, and glycine betaine, are considered the major contributing factors that work as osmolytes and osmoprotectants for plants from various environmental stress factors. In contrast, plant-derived secondary metabolites, e.g., phenolics, terpenoids, and nitrogen-containing compounds (alkaloids), have no direct role in the growth and development of plants. Nevertheless, such metabolites could play a significant role as a defense by protecting plants from biotic factors such as herbivores, insects, and pathogens. In addition, they can enhance the resistance against abiotic factors. Therefore, metabolomics practices are becoming essential and influential in plants by identifying different phytochemicals that are part of the acclimation responses to various stimuli. Hence, an accurate metabolome analysis is important to understand the basics of stress physiology and biochemistry. This review provides insight into the current information related to the impact of biotic and abiotic factors on variations of various sets of metabolite levels and explores how primary and secondary metabolites help plants in response to these stresses.

This study explores the role of religion as a state-promoted tool for political assimilation in Pakistan’s border provinces of Khyber Pakhtunkhwa (KP) and Balochistan. The study is based on five phases of fieldwork (2016–2024) combined with a thematic literature review. The research explores how religious strategies were deployed to forge a unified national identity in these regions. The findings reveal significant disparities in the effectiveness of these strategies. In KP, historical factors, cultural alignment, and geopolitical influences—particularly the Afghan conflict—largely facilitated the integration of Pashtun identity into Pakistan’s broader Islamic-national framework. Tools such as madrassa networks, education reforms, religious slogans, and state-backed Islamist parties effectively promoted religious nationalism. In contrast, religious assimilation efforts in Balochistan largely failed due to entrenched ethnic nationalism, economic exclusion, and political marginalization. Attempts to expand madrassas, delegitimize nationalist leaders as “anti-Islamic,” and support religious movements have been met with resistance, deepening distrust between the Baloch population and the state. The study found that religion alone cannot sustain national cohesion, particularly in regions with longstanding grievances and systemic inequalities. This research emphasizes the limitations of top-down, coercive assimilationist policies and underscores the necessity for more inclusive approaches, such as addressing economic disparities, recognizing regional identities, and promoting political participation as essential components for building a sustainable and unified nation. The study provides critical insights for policymakers, advocating for a shift from religious assimilation to strategies that prioritize justice, equity, and cultural accommodation, particularly in KPK and Balochistan.

This review article is built on the beneficial effects of Lactobacillus against different diseases, and a special focus has been made on its effects against neurological disorders, such as depression, multiple sclerosis, Alzheimer’s, and Parkinson’s disease. Probiotics are live microbes, which are found in fermented foods, beverages, and cultured milk and, when administered in an adequate dose, confer health benefits to the host. They are known as “health-friendly bacteria”, normally residing in the human gut and involved in maintaining homeostatic conditions. Imbalance in gut microbiota results in the pathophysiology of several diseases entailing the GIT tract, skin, immune system, inflammation, and gut–brain axis. Recently, the use of probiotics has gained tremendous interest, because of their profound effects on the management of these disease conditions. Recent findings suggest that probiotics enrichment in different human and mouse disease models showed promising beneficial effects and results in the amelioration of disease symptoms. Thus, this review focuses on the current probiotics-based products, different disease models, variable markers measured during trials, and evidence obtained from past studies on the use of probiotics in the prevention and treatment of different diseases, covering the skin to the central nervous system diseases.

Efficient wound and burn healing is crucial for minimising complications, preventing infections, and enhancing overall well-being, necessitating the development of innovative strategies. This study aimed to formulate a novel thin film combining chitosan, carboxymethyl cellulose, tannic acid, and beeswax for improved wound healing applications. Several formulations, incorporating chitosan, carboxymethyl cellulose, tannic acid, and beeswax in various percentages, were utilized to deposit thin films via the solvent evaporation technique, Mechanical properties, morphology, antioxidant activity, antibacterial efficacy, and wound healing potential were evaluated. The optimized thin film (M4), composed of 2% chitosan, 2% carboxymethyl cellulose, and 1% tannic acid, along with 0.2% glycerol and 0.2% tween80, exhibited a thickness of 39.0 ± 1.14 μm and a tensile strength of 0.275 ± 0.003 MPa. It demonstrated a swelling degree of 283.0 ± 2.0% and a drug release capacity of 89.4% within 24 h. The film also showed a low contact angle of 40.5° and a water vapour transmission rate of 1912.25 ± 13.10 g m −2 0.24 h −1 . FT-IR spectroscopy indicated that chitosan and carboxymethyl cellulose were cross-linked through amide linkages, with tannic acid occupying the interstitial spaces and hydrogen bonding stabilizing the structure. Microscopy of M4 revealed a uniform morphology. The film exhibited strong antioxidant activity of (95.17 ± 0.02%) and antibacterial efficiency (80.8%) against S. aureus . In a rabbit model, the film significantly enhanced burn and excision wound recovery, with 90.0 ± 3.3% healing for burns and 88.85 ± 1.7% for infected wounds by day 7. Complete skin regeneration was observed within 10–12 days. The M4 thin film demonstrated exceptional mechanical properties and bioactivity, offering protection against pathogens and promoting efficient wound healing. These findings suggest its potential for further investigation in treating various infections and its role in developing novel therapeutic interventions.

The study investigated the adverse impact of medical waste on Indigenous communities and explored sustainable solutions to reduce inequality and enhance resilience. We adopted a qualitative thematic analysis from primary data collected from 176 respondents. Data were collected through focus groups and interviews. In the research, we examined the interconnected themes challenging medical waste management practices in Pakistan, such as poor disposal practices, health and environmental hazards of poor medical waste management through disease transmission and infections, and soil and water contamination. Socioeconomic disparities and inequality were also identified, resulting in economic burdens on vulnerable groups-the theme of which was Indigenous communities disadvantaged through health risks and vulnerabilities and disproportionate impact on health and well‑being. Themes further highlight government efforts, suggested regulatory and policy reforms, capacity building, and awareness. We developed three significant recommendations for sustainable solutions to reducing inequality and improving community resilience. The first is community empowerment and awareness, emphasizing the need to educate community members, healthcare professionals, and waste handlers about the risks of improper medical waste disposal. The second is strengthening infrastructure and collaboration, highlighting the urgent need to establish proper waste collection and segregation infrastructure. Collaboration among healthcare facilities, waste management agencies, government bodies, and community leaders is instrumental in designing comprehensive solutions that meet the unique needs of Indigenous communities. The third one is policy enhancement and enforcement, suggesting the importance of policy revisions and rigorous enforcement mechanisms. The study advocates for policies that reflect current challenges and encourage innovative approaches to medical waste management.

Light Fidelity (LiFi) is a new candidate for wireless networking that utilizes the visible light spectrum and exploits the existing lighting infrastructure in the form of light-emitting diodes (LEDs). It provides point-to-point and point-to-multipoint communication on a bidirectional channel at very high data rates. However, the LiFi has small coverage, and its optical gain is closely related to the receiver’s directionality vis-à-vis the transmitter, therefore it can experience frequent service outages. To provide reliable coverage, the LiFi is integrated with other networking technologies such as wireless fidelity (WiFi) thus forming a hybrid system. The hybrid LiFi/WiFi system faces many challenges including but not limited to seamless integration with the WiFi, support for mobility, handover management, resource sharing, and load balancing. The existing literature has addressed one or the other aspect of the issues facing LiFi systems. There are limited free source tools available to holistically address these challenges in a scalable manner. To this end, we have developed an open-source simulation framework based on the network simulator 3 (ns-3), which realizes critical aspects of the LiFi wireless network. Our developed ns-3 LiFi framework provides a fully functional AP equipped with the physical layer and medium access control (MAC), a mobility model for the user device, and integration between LiFi and WiFi with a handover facility. Simulation results are produced to demonstrate the mobility and handover capabilities, and the performance gains from the LiFi-WiFi hybrid system in terms of packet delay, throughput, packet drop ratio (PDR), and fairness between users. The source code of the framework is made available for the use of the research community.

In limit of saturation spectroscopy, we theoretically study the spectral hole burning (SHB) in the absorption spectrum of a probe field through a tripod atomic system. The response function for the probe field is calculated in a Doppler-broadened medium. Burning of spectral holes is observed only for the counter propagation of either one or both the coupling fields in the medium. The SHB is not observed below some critical temperature which is a condition for the electromagnetically induced transparency (EIT) in the medium. The most interesting and significant feature is that the Doppler broadening acts as a decoherence effect in case of EIT, however, the Doppler broadening acts inversely in case of SHB and consequently the burning effect enhances. The SHB is further enhanced and controlled by classes of the average velocity of atoms. The classes of high average atomic velocity in the medium increase the number of spectral hole burns (HBs). The widths of HBs can be controlled by the intensity of the driving fields. A single HB can be switched to multiple HBs in a well-controlled manner using different classes of high average atomic velocity. The various switchable holes can be burned in a desired position of the absorption spectrum which in turn simultaneously slow down multiple probe fields. The phenomenon of SHB may be useful in the construction of multichannel optical switching and storage devices.