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Lithium chloride (LiCl) has emerged as a promising alternative to synthetic acaricides for controlling Varroa destructor , a major threat to honey bee health. However, its potential side effects on bees and their products require further investigation. This study aimed to assess the effects of LiCl on honey bee health, focusing on survival, behavior, and molecular responses. We investigated the effects of varying doses and feeding durations of LiCl on honey bee survival, aggression, stress- and immune-related gene expression, and recovery potential after treatment cessation. Our results show that LiCl feeding reduced honey bee survival in a dose-dependent manner, with higher concentrations leading to greater accumulation of lithium in bee tissues. Furthermore, LiCl suppressed aggression behavior and altered the gene expression related to honey bee health, such as vitellogenin, antimicrobial peptides, antioxidant enzymes, and heat shock proteins. The duration of LiCl feeding was a critical factor, as shorter feeding periods followed by recovery with control diets restored gene expression and survival rates to the levels of control groups. These findings highlight the importance of optimizing LiCl dosage and feeding duration to balance its Varroa controlling efficacy with honey bee safety, and further research is needed to ensure its long-term safety for colonies and ecosystems.

The honey bee (Apis mellifera) faces a significant threat from Varroa destructor, causing the losses of millions of colonies worldwide. While synthetic acaricides are widely used to control Varroa infestations, excessive application has led to resistant strains and poses side effects on the host. Consequently, there is an urgent need for a new acaricide that is both effective and affordable, yet safe to use on bees. One potential source of these acaricides is essential oils (EOs) and their constituents. This study evaluated the acaricidal properties of four essential oils (Eucalyptus globulus, Rosemary officinalis, Trachyspermum ammi (Ethiopian and Indian varieties), their constituents and mixture of constituents against V. destructor through the complete exposure method. Our finding showed that a 1:1 mixture of thymol and carvacrol (4 h-LC50 = 42 μg/mL), thymol (4 h-LC50 = 71 μg/mL), and T. ammi oil (4 h-LC50 = 81–98 μg/mL) were the most toxic test samples against V. destructor. Honey bee behavior and selectivity were also assessed with one additional EO Thymus schimperi, indicating that T. schimperi, T. ammi, and their components were selective and did not affect the learning and memory of bees. In conclusion, the thymol and carvacrol (1:1) mixture was shown to be a promising replacement for synthetic acaricides, being three times more toxic than a commercial acaricide, fluvalinate (4 h-LC50 = 143 μg/mL).

Honey bees are essential pollinators in global food production, however, their populations are increasingly threatened by insecticides. Protecting bees from these chemical stressors is critical not only for ecosystem stability but also for agricultural sustainability. Natural dietary compounds, such as curcumin (CU) and rosmarinic acid (RA), have demonstrated antioxidant and detoxification-promoting properties in other organisms and may offer a promising approach to enhancing honey bee resilience to pesticide exposure. This study investigates the potential of CU and RA to mitigate pesticide-induced harm in honey bees. In acute toxicity tests, newly emerged bees and foragers were topically exposed to lethal doses of acetamiprid (1.04 µg/bee for newly emerged and 15.3 µg/bee for forager), carbaryl (0.06 µg/bee for newly emerged and 0.51 µg/bee for forager), and flupyradifurone (15.6 µg/bee for newly emerged and 24.1 µg/bee for forager), followed by post-feeding with CU and RA at 50, 100, and 200 ppm for 48h. Additionally, the effects of CU and RA at 100 ppm were tested under chronic oral intoxication through continuous insecticide feeding. CU100 significantly reduced mortality in insecticide-exposed bees, except foragers exposed to acetamiprid, while RA showed variable detoxification effects, with RA100 and RA200 improving survival in carbaryl-exposed bees and RA50 enhancing survival of 0.06 µg/bee for newly emerged bees exposed to flupyradifurone. Chronic toxicity assessments confirmed CU100’s superior protective effect over RA100, especially in carbaryl-exposed groups. Gene expression analysis revealed that CU and RA modulated detoxification related genes, enhancing honey bees' resilience by upregulating key detoxification genes in the head and abdomen. These findings suggest that CU and RA offer potential benefits in reducing insecticide toxicity in honey bees. However, further research is needed to assess their effects across different life stages, environmental conditions, and colony dynamics, as well as to elucidate the pathways involved in detoxification gene regulation. A comprehensive understanding of their mechanisms and ecological implications is essential before considering these compounds for practical applications in pollinator health management.

Honey bees (Apis mellifera) that play vital roles in pollination and ecosystem maintenance, face severe threats from the ectoparasite, Varroa destructor. Existing control techniques, including mechanical, chemical, and organic, have had adverse effects on honey bees. Therefore, finding an easy, effective, affordable, and safe method is crucial. Essential oils (EOs) and their major components emerge as potential candidates due to their higher efficiency, biodegradability, and selectivity. However, evaluating composition variability, as well as their efficiency and safety in honey bee species, is essential. In this study, we assessed the efficiency of essential oil and their components against Varroa mites while studying the safety for honey bees. Eucalyptus globulus, Rosemary officinalis, Trachyspermum ammi (Ethiopian and Indian varieties), alongside their major components and a 1:1 mixture, were assessed for their acaricidal activity. All the samples exhibited acaricidal activity, with T. ammi, thymol, and the 1:1 mixture of thymol and carvacrol showing the highest efficiency against V. destructor. Importantly, the EOs and their major components showed selectivity and did not affect the honey bees’ learning and memory. In conclusion, our findings highlight the potential of T. ammi and the 1:1 mixture of thymol and carvacrol as candidates for Varroa control, suggesting further study at the colony level. The honey bee (Apis mellifera) faces a significant threat from Varroa destructor, causing the losses of millions of colonies worldwide. While synthetic acaricides are widely used to control Varroa infestations, excessive application has led to resistant strains and poses side effects on the host. Consequently, there is an urgent need for a new acaricide that is both effective and affordable, yet safe to use on bees. One potential source of these acaricides is essential oils (EOs) and their constituents. This study evaluated the acaricidal properties of four essential oils (Eucalyptus globulus, Rosemary officinalis, Trachyspermum ammi (Ethiopian and Indian varieties), their constituents and mixture of constituents against V. destructor through the complete exposure method. Our finding showed that a 1:1 mixture of thymol and carvacrol (4 h-LC[sub.50] = 42 μg/mL), thymol (4 h-LC[sub.50] = 71 μg/mL), and T. ammi oil (4 h-LC[sub.50] = 81–98 μg/mL) were the most toxic test samples against V. destructor. Honey bee behavior and selectivity were also assessed with one additional EO Thymus schimperi, indicating that T. schimperi, T. ammi, and their components were selective and did not affect the learning and memory of bees. In conclusion, the thymol and carvacrol (1:1) mixture was shown to be a promising replacement for synthetic acaricides, being three times more toxic than a commercial acaricide, fluvalinate (4 h-LC[sub.50] = 143 μg/mL).