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Habitat connectivity and trophic shifts during the lifetime of an individual fish are important determinants of fish population growth and persistence, yet remain little understood for many species. We investigated whether insights into individual lifetime migration, trophic position (TP) and environmental nitrogen dynamics could be achieved using compound-specific nitrogen isotope analysis of otolith proteinogenic amino acids (AAs). By comparing acoupa weakfish Cynoscion acoupa otoliths and muscle tissue from the monsoonal Amazon area in Pará with otoliths from semi-arid Rio Grande do Norte (RGN), Brazil, this study illustrates estuarine to coastal shelf habitat use and trophic ecology during juvenile and adult stage growth. Muscle tissue and otoliths gave comparable TPs for both life stages, while weighted mean δ15N values of all source AAs differed between tissues. These differences reflected large seasonal and spatial changes in nitrogen biogeochemical cycles and anthropogenic nitrogen influences from the Amazon River onto the coastal shelf of Pará. AA δ15N values of fish otoliths from the Pará region indicated changes in TP and sources of nitrogen between life stages, whereas analysis of fish otoliths from the RGN region indicated similarities in individual TP and sources of nitrogen through ontogeny. However, in both areas, individual adult TP ranged between 3 and 4, whereas juvenile TP remained around 2.8 to 3.0 in Pará and RGN, respectively. Since otoliths preserve a record of baseline δ15N values over the lifetime of individual fish it may be possible to infer migration and TP across prehistoric ecosystems from AA isotopic analysis of ancient otoliths.

Habitat connectivity and trophic shifts during the lifetime of an individual fish are important determinants of fish population growth and persistence, yet remain little understood for many species. We investigated whether insights into individual lifetime migration, trophic position (TP) and environmental nitrogen dynamics could be achieved using compound-specific nitrogen isotope analysis of otolith proteinogenic amino acids (AAs). By comparing acoupa weakfish Cynoscion acoupa otoliths and muscle tissue from the monsoonal Amazon area in Pará with otoliths from semi-arid Rio Grande do Norte (RGN), Brazil, this study illustrates estuarine to coastal shelf habitat use and trophic ecology during juvenile and adult stage growth. Muscle tissue and otoliths gave comparable TPs for both life stages, while weighted mean δ15N values of all source AAs differed between tissues. These differences reflected large seasonal and spatial changes in nitrogen biogeochemical cycles and anthropogenic nitrogen influences from the Amazon River onto the coastal shelf of Pará. AA δ15N values of fish otoliths from the Pará region indicated changes in TP and sources of nitrogen between life stages, whereas analysis of fish otoliths from the RGN region indicated similarities in individual TP and sources of nitrogen through ontogeny. However, in both areas, individual adult TP ranged between 3 and 4, whereas juvenile TP remained around 2.8 to 3.0 in Pará and RGN, respectively. Since otoliths preserve a record of baseline δ15N values over the lifetime of individual fish it may be possible to infer migration and TP across pre historic ecosystems from AA isotopic analysis of an cient otoliths.

With the increasing anthropogenic impacts on fish habitats, it has become more important to understand which primary resources sustain fish populations. This resource utilization can differ between fish life stages, and individuals can migrate between habitats in search of resources. Such lifetime information is difficult to obtain due to the large spatial and temporal scales of fish behavior. The otolith organic matrix has the potential to indicate this resource utilization and migration with δ13C values of essential amino acids (EAAs), which are a direct indication of the primary producers. In a proof-of-concept study, we selected the Acoupa weakfish, Cynoscion acoupa, as a model fish species with distinct ontogenetic migration patterns. While it inhabits the Brazilian mangrove estuaries during juvenile stages, it moves to the coastal shelf as an adult. Thus, we expected that lifetime resource utilization and migration would be reflected in δ13CEAA patterns and baseline values in C. acoupa otoliths. By analyzing the C. acoupa otolith edges across a size range of 12–119 cm, we found that baseline δ13CEAA values increased with size, which indicated an estuarine to coastal shelf distribution. This trend is highly correlated with inorganic δ13C values. The δ13CEAA patterns showed that estuarine algae rather than mangrove-derived resources supported the juvenile C. acoupa populations. Around the juvenile size of 40 cm, resource utilization overlapped with those of adults and mean baseline δ13CEAA values increased. This trend was confirmed by comparing otolith core and edges, although with some individuals potentially migrating over longer distances than others. Hence, δ13CEAA patterns and baseline values in otoliths have great potential to reconstruct ontogenetic shifts in resource use and habitats. The insight could aid in predictions on how environmental changes affect fish populations by identifying the controlling factors at the base of the food web.

With the increasing anthropogenic impacts on fish habitats, it has become more important to understand which primary resources sustain fish populations. This resource utilization can differ between fish life stages, and individuals can migrate between habitats in search of resources. Such lifetime information is difficult to obtain due to the large spatial and temporal scales of fish behavior. The otolith organic matrix has the potential to indicate this resource utilization and migration with δ C values of essential amino acids (EAAs), which are a direct indication of the primary producers. In a proof-of-concept study, we selected the Acoupa weakfish, as a model fish species with distinct ontogenetic migration patterns. While it inhabits the Brazilian mangrove estuaries during juvenile stages, it moves to the coastal shelf as an adult. Thus, we expected that lifetime resource utilization and migration would be reflected in δ C patterns and baseline values in otoliths. By analyzing the otolith edges across a size range of 12-119 cm, we found that baseline δ C values increased with size, which indicated an estuarine to coastal shelf distribution. This trend is highly correlated with inorganic δ C values. The δ C patterns showed that estuarine algae rather than mangrove-derived resources supported the juvenile populations. Around the juvenile size of 40 cm, resource utilization overlapped with those of adults and mean baseline δ C values increased. This trend was confirmed by comparing otolith core and edges, although with some individuals potentially migrating over longer distances than others. Hence, δ C patterns and baseline values in otoliths have great potential to reconstruct ontogenetic shifts in resource use and habitats. The insight could aid in predictions on how environmental changes affect fish populations by identifying the controlling factors at the base of the food web.

BackgroundThe ACOSOG Z0011 trial showed that completion axillary lymph node dissection (cALND) can be safely omitted for some patients with T1–2 clinically node-negative breast cancer with one to two involved sentinel lymph nodes (SLNs) treated with breast-conserving therapy (BCT). There is little evidence for the safety of omitting cALND for mastectomy-treated patients. Consequently, cALND is often recommended for sentinel node-positive patients treated with mastectomy. The aim of this study is to determine the proportion of patients who could avoid cALND by choosing BCT instead of mastectomy at a tertiary cancer center.Patients and MethodsAll T1–2 clinically node-negative breast cancer patients treated with BCT or mastectomy between 2012 and 2017 with metastases in the SLN(s) were selected from a prospectively maintained database. Clinical factors and outcomes were evaluated between the two groups. Differences were compared using Wilcoxon rank-sum test, chi-square test or Fisher’s exact test as appropriate. Significance was set at the 0.05 level for all analyses.ResultsA total of 306 patients were included, 199 (65.0%) of whom were treated with BCT and 107 (35.0%) with mastectomy. Patients treated with mastectomy were more often treated with cALND compared with those treated with BCT (71.0% versus 26.6%, p < 0.0001). Overall, 52 of the mastectomy patients (68.4%) could have avoided cALND if they had chosen BCT.ConclusionsPatients treated with mastectomy are more likely to receive cALND than those treated with BCT. Axillary management should be addressed during discussion of primary tumor therapy, and cALND may be avoided when patients choose BCT instead of mastectomy.

Conservation paleobiology seeks to leverage proxy reconstructions of ecological communities and environmental conditions to predict future changes and inform management decisions. Populations of East African megafauna likely changed during the Holocene in response to trends and events in the regional hydroclimate, but reconstructing these populations requires development of new proxies. We examine if fecal steroids are a viable proxy for megafauna populations since they are well preserved in sedimentary archives. We measured eleven fecal steroids in 87 fresh dung samples representing 22 species of megafauna in the Maasai Mara National Reserve (Kenya) and a further seven samples from captive animals. Using this reference library, four distinctive groups are identified, which reflect diet and biochemical modification of these inputs during digestion by the gut microbiome. Carnivore dung is characterized by more than ~ 75% cholesterol and primate dung includes uniquely high proportions of coprostanol. Two groups of herbivore are distinguished by their differing proportions of phytosterols that are consumed by eating plants and 5β-stanols produced during digestion. Under cross validation a random forests statistical model accurately classified 72% of dung samples to the species level using fecal steroids. Variability among individuals and between wild and captive animals suggests that fecal steroids in herbivore dung may reflect diversity and variability in diet, while a lack of variability in carnivore dung indicates that they cannot be identified to the species level in most instances. Our results suggest that fecal steroids may have utility in reconstructing the time-evolving composition megafauna populations in East Africa.

Biomonitoring of contaminants (metals, organotins, polyaromatic hydrocarbons (PAHs), PCBs) was undertaken in Milford Haven Waterway (MHW) and a reference site in the Tywi Estuary (St Ishmael/Ferryside) during 2007–2008. Bioindicator species encompassed various uptake routes— Fucus vesiculosus (dissolved contaminants); Littorina littorea (grazer); Mytilus edulis and Cerastoderma edule (suspension feeders); and Hediste (= Nereis ) diversicolor (sediments). Differences in feeding and habitat preference have subtle implications for bioaccumulation trends though, with few exceptions, contaminant burdens in MHW were higher than the Tywi reference site, reflecting inputs. Elevated metal concentrations were observed at some MHW sites, whilst As and Se (molluscs and seaweed) were consistently at the higher end of the UK range. However, for most metals, distributions in MH biota were not exceptional. Several metal-species combinations indicated increases in bioavailability upstream, which may reflect the influence of geogenic/land-based sources—perhaps enhanced by lower salinity. TBT levels in MH mussels were below OSPAR toxicity thresholds and in the Tywi were close to zero. Phenyltins were not accumulated appreciably in M. edulis , whereas some H. diversicolor populations appear subjected to localized (historical) sources. PAHs in H. diversicolor were distributed evenly across most of MHW, although acenaphthene, fluoranthene, pyrene, benzo( a )anthracene and chrysene were highest at one site near the mouth; naphthalenes in H. diversicolor were enriched in the mid-upper Haven (a pattern seen in M. edulis for most PAHs). Whilst PAH (and PCB) concentrations in MH mussels were mostly above reference and OSPAR backgrounds, they are unlikely to exceed ecotoxicological thresholds. Bivalve Condition indices (CI) were highest at the Tywi reference site and at the seaward end of MH, decreasing upstream—giving rise to several significant (negative) relationships between CI and body burdens. Despite the possible influence of salinity gradient as a complicating factor, multivariate analysis indicated that a combination of contaminants could influence the pattern in condition (and the biomarkers metallothionein and TOSC). Integrating bioaccumulation data with biological and biochemical endpoints is seen as a useful way to discriminate environmental quality of moderately contaminated areas such as MHW and to prioritise cause and effect investigations.

Surface sediments from a 160-km stretch of the River Clyde, Scotland, were analysed for persistent organic pollutants to investigate distribution, source and environmental effect. Glasgow's urban tributaries polyaromatic hydrocarbons (PAH) ranged from 2.3 to 4226mgkg–1, total petroleum hydrocarbons (TPH) 72 to 37879mgkg–1 and polychlorinated biphenyls (PCB) 3 to 809μgkg–1, which were more polluted than the upper River Clyde PAH that ranged from 0.1 to 42mgkg–1, TPH 3 to 260mgkg–1 and PCB 2 to 147μgkg–1. Intermediate values of the inner Clyde estuary PAH ranging from 0.6 to 30mgkg–1, and PCB ranging from 5 to 130μgkg–1, were attributed to point sources and sediment transfer from the urban tributaries. Comparison with sediment quality criteria suggested possible adverse effects on aquatic biota. PAH isomeric ratios confirmed a pyrolytic source throughout the Clyde and benzo[a]pyrene/benzo[g,h,i]perylene ratios >0.6 confirmed that upper, urban and estuarine domains all to a lesser or greater extent accumulated PAH from traffic emissions. The degree of chlorination determined from PCB homologues differed in each of the three domains, suggesting variable source or that the process aerobic/anaerobic degradation varied in each of the three domains. The anthropogenic impact of the city of Glasgow can be quantified in that the urban tributary sediment mean values were 60 (PAH), 33 (TPH) and 11 (PCB) times higher than the rural upper Clyde counterpart.

Concentrations of total organic carbon (TOC), total petroleum hydrocarbons, polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) were determined in 84 near-surface soils (5–20cm depth) taken from a 255km2 area of Glasgow in the Clyde Basin, UK, during July 2011. Total petroleum hydrocarbon range was 79–2,505mgkg–1 (mean 388mgkg–1; median 272mgkg–1) of which the aromatic fraction was 13–74 % (mean 44 %, median 43 %) and saturates were 28–87 % (mean 56 %, median 57 %). ∑16 PAH varied from 2–653mgkg–1 (mean 32.4mgkg–1; median 12.5mgkg–1) and ∑31 PAH range was 2.47–852mgkg–1 (mean 45.4mgkg–1; median 19.0mgkg–1). ∑PCBtri-hepta range was 2.2–1052μgkg–1 (mean 32.4μgkg–1; median 12.7μgkg–1) and the ∑PCB7 range was 0.3–344μgkg–1 (mean 9.8μgkg–1; median 2.7μgkg–1). The concentration, distribution and source of the persistent organic pollutants were compared with those found in urban soils from other cities and to human health assessment criteria for chronic exposure to chemicals in soil. Total concentrations encountered were generally similar to other urban areas that had a similar industrial history. Benzo[a]pyrene concentrations were assessed against four different land use scenarios (irrespective of current land use) using generic assessment criteria resulting in six of 84 samples exceeding the residential criteria. Isomeric PAH ratios and relative abundance of perylene suggest multiple and environmentally modified pyrogenic PAH sources, inferred to be representative of diffuse pollution. ∑PCB7 concentrations were exceeded in 10 % of sites using the Dutch target value of 20μgkg–1. PCB congener profiles were environmentally attenuated and generally dominated by penta-, hexa- and hepta-chlorinated congeners.