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Gastric-type cervical adenocarcinoma (GCA) is an aggressive type of endocervical adenocarcinoma characterized by mucinous morphology, gastric-type mucin, lack of association with human papillomavirus (HPV) and resistance to chemo/radiotherapy. We characterized the landscape of genetic alterations in a large cohort of GCAs, and compared it with that of usual-type HPV-associated endocervical adenocarcinomas (UEAs), pancreatic adenocarcinomas (PAs) and intestinal-type gastric adenocarcinomas (IGAs). GCAs (n = 68) were subjected to massively parallel sequencing targeting 410–468 cancer-related genes. Somatic mutations and copy number alterations (CNAs) were determined using validated bioinformatics methods. Mutational data for UEAs (n = 21), PAs (n = 178), and IGAs (n = 148) from The Cancer Genome Atlas (TCGA) were obtained from cBioPortal. GCAs most frequently harbored somatic mutations in TP53 (41%), CDKN2A (18%), KRAS (18%), and STK11 (10%). Potentially targetable mutations were identified in ERBB3 (10%), ERBB2 (8%), and BRAF (4%). GCAs displayed low levels of CNAs with no recurrent amplifications or homozygous deletions. In contrast to UEAs, GCAs harbored more frequent mutations affecting cell cycle-related genes including TP53 (41% vs 5%, p < 0.01) and CDKN2A (18% vs 0%, p = 0.01), and fewer PIK3CA mutations (7% vs 33%, p = 0.01). TP53 mutations were less prevalent in GCAs compared to PAs (41% vs 56%, p < 0.05) and IGAs (41% vs 57%, p < 0.05). GCAs showed a higher frequency of STK11 mutations than PAs (10% vs 2%, p < 0.05) and IGAs (10% vs 1%, p < 0.05). GCAs harbored more frequent mutations in ERBB2 and ERBB3 (9% vs 1%, and 10% vs 0.5%, both p < 0.01) compared to PAs, and in CDKN2A (18% vs 1%, p < 0.05) and KRAS (18% vs 6%, p < 0.05) compared to IGAs. GCAs harbor recurrent somatic mutations in cell cycle-related genes and in potentially targetable genes, including ERBB2/3. Mutations in genes such as STK11 may be used as supportive evidence to help distinguish GCAs from other adenocarcinomas with similar morphology in metastatic sites.

Background The variable incidence of gallbladder cancer (GBCA) suggests regional pathogenetic differences. This study compares cell cycle-regulatory, angiogenesis-related, and PI3K pathway protein expression in GBCAs from three continents. Methods Immunohistochemical expression of several proteins was assessed, correlated with clinicopathologic variables, and compared among centers from Chile (Fundación Arturo López Pérez [FALP]), Japan (Yokohama City University [YCU]), and the United States (Memorial Sloan-Kettering Cancer Center [MSKCC]). Hierarchical clustering was used to partition the data based on protein-expression and treatment center. Results Tissue from 117 patients (MSKCC = 76; FALP = 22; YCU = 19) was analyzed. Mdm2 overexpression was seen only at MSKCC ( p  < 0.0001). Absence of p21 ( p  = 0.03) and VEGFR2 ( p  = 0.018) were more common and p27 expression was less frequent ( p  = 0.047) in tumors from YCU. Ki-67 labeling index in YCU tumors (median = 10) was two-thirds lower than at other centers. On hierarchical clustering analysis, all YCU patients ( p  = 0.017) and those with early tumors ( p  = 0.017) clustered separately from MSKCC. Median disease-specific survival after curative intent (R0) resection was 27 months and was similar among centers ( p  = 0.9). Median disease-specific survival of patients with early tumors was 28.4 months and was higher at YCU (not reached, p  = 0.06). Conclusions Cell cycle-regulatory protein expression patterns of YCU tumors differed from those treated at FALP and MSKCC. The differential clustering of protein expression and survival in patients with early tumors suggest regional differences in pathogenesis and disease biology.

Most cases of pancreatic cancer are not diagnosed until they are no longer curable with surgery. Therefore, it is critical to develop a sensitive, preferably noninvasive, method for detecting the disease at an earlier stage. In order to identify biomarkers for pancreatic cancer, we devised an in vitro positive/negative selection strategy to identify RNA ligands (aptamers) that could detect structural differences between the secretomes of pancreatic cancer and non-cancerous cells. Using this molecular recognition approach, we identified an aptamer (M9-5) that differentially bound conditioned media from cancerous and non-cancerous human pancreatic cell lines. This aptamer further discriminated between the sera of pancreatic cancer patients and healthy volunteers with high sensitivity and specificity. We utilized biochemical purification methods and mass-spectrometric analysis to identify the M9-5 target as cyclophilin B (CypB). This molecular recognition-based strategy simultaneously identified CypB as a serum biomarker and generated a new reagent to recognize it in body fluids. Moreover, this approach should be generalizable to other diseases and complementary to traditional approaches that focus on differences in expression level between samples. Finally, we suggest that the aptamer we identified has the potential to serve as a tool for the early detection of pancreatic cancer.

Objective: This study aimed to describe the predisposing causes for pregnancy among adolescents and theirknowledge on the prevention methods. Method: This is an exploratory, descriptive, and quantitative research carried out at basic health units in the town of Sao Caetano, Pernambuco, Brazil, in September and October 2011. For data collection, one used a semi-structured questionnaire applied to 42 adolescents enrolled for prenatal care. Results: The findings show that most adolescents was around 16 years of age, brown skinned, literate, and Catholic, lived with her partner, and had a low socioeconomic status. One’s own wish (54.8%) stood out as the main cause for pregnancy in the population under study. Conclusion: One found out there’s a need for investing on strategies for providing these female adolescents with guidance, so that contraceptive practices and the responsible exercise of sexuality start being realized as positive and usual behaviors.

Objective: This study aimed to describe the predisposing causes for pregnancy among adolescents and theirknowledge on the prevention methods. Method: This is an exploratory, descriptive, and quantitative research carried out at basic health units in the town of Sao Caetano, Pernambuco, Brazil, in September and October 2011. For data collection, one used a semi-structured questionnaire applied to 42 adolescents enrolled for prenatal care. Results: The findings show that most adolescents was around 16 years of age, brown skinned, literate, and Catholic, lived with her partner, and had a low socioeconomic status. One’s own wish (54.8%) stood out as the main cause for pregnancy in the population under study. Conclusion: One found out there’s a need for investing on strategies for providing these female adolescents with guidance, so that contraceptive practices and the responsible exercise of sexuality start being realized as positive and usual behaviors.

Objective: This study aimed to describe the predisposing causes for pregnancy among adolescents and theirknowledge on the prevention methods. Method: This is an exploratory, descriptive, and quantitative research carried out at basic health units in the town of Sao Caetano, Pernambuco, Brazil, in September and October 2011. For data collection, one used a semi-structured questionnaire applied to 42 adolescents enrolled for prenatal care. Results: The findings show that most adolescents was around 16 years of age, brown skinned, literate, and Catholic, lived with her partner, and had a low socioeconomic status. One’s own wish (54.8%) stood out as the main cause for pregnancy in the population under study. Conclusion: One found out there’s a need for investing on strategies for providing these female adolescents with guidance, so that contraceptive practices and the responsible exercise of sexuality start being realized as positive and usual behaviors.

Objective: This study aimed to describe the predisposing causes for pregnancy among adolescents and theirknowledge on the prevention methods. Method: This is an exploratory, descriptive, and quantitative research carried out at basic health units in the town of Sao Caetano, Pernambuco, Brazil, in September and October 2011. For data collection, one used a semi-structured questionnaire applied to 42 adolescents enrolled for prenatal care. Results: The findings show that most adolescents was around 16 years of age, brown skinned, literate, and Catholic, lived with her partner, and had a low socioeconomic status. One’s own wish (54.8%) stood out as the main cause for pregnancy in the population under study. Conclusion: One found out there’s a need for investing on strategies for providing these female adolescents with guidance, so that contraceptive practices and the responsible exercise of sexuality start being realized as positive and usual behaviors.