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63
result(s) for
"Verma, P.S."
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Design, simulation and experimental study of near-field beam forming techniques using conformal waveguide arrays
2010
Microwave hyperthermia is rapidly evolving as a fourth modality in the fight against cancer, along with surgery, radiation and chemotherapy. This form of cancer treatment utilises a narrow microwave beam to heat the tumour volume to a temperature of ∼42°C; however, with minimal energy delivery to neighbouring healthy tissue, which is one of the main challenges in hyperthermia technology. This paper describes another approach to near-field beam forming by using of a conformal waveguide array, operating in the K band (18 GHz-26 GHz). The focusing element gives conformal property to the array and serves two purposes: firstly, to obtain a sharp focus at a prescribed near-field location, and secondly, the added flexibility to move the beam around the tumour. Several simulations and measurements have been performed on linear and planar configurations, which demonstrate the ability of the array to achieve beam widths as small as ∼4 mm, with a maximum beam movement range of ∼15 mm.
Journal Article
Hulless barley as a promising source to improve the nutritional quality of wheat products
by
Verma, Ramesh Pal Singh
,
Kumar, Dinesh
,
Gupta, R. K
in
antioxidant activity
,
Antioxidants
,
Barley
2017
In this study, efforts were made to utilize hulless barley (variety BHS352) to enhance the nutritive value of chapatti and biscuit made from wheat flour. Barley flour was added to wheat flour in different ratios (5 to 30%). Antioxidant activity, total phenolic content and β-glucan content were determined both in flour blends and their products. Changes in physical quality and taste of chapatti and biscuits after blending of hulless barley flour with wheat flour were measured. The chapatti quality score decreased by 15% and biscuit spread factor by 33% after 30% barley flour blending. Significant increase in β-glucan content and antioxidant activity of flour blends and their products was observed at 30% blending level. The phenolic content increased from 63 to 135 µg for biscuits and 237 to 287 ug GAE/g for chapatti with blending of 30% barley flour.
Journal Article
Genome Wide Association Mapping of Seedling and Adult Plant Resistance to Barley Stripe Rust (Puccinia striiformis f. sp. hordei) in India
by
Shekhawat, P.S
,
Bhardwaj, Subhash Chand
,
Al-Abdallat, Ayed
in
adult plant resistance (APR)
,
Barley
,
barley (Hordeum vulgare L.)
2018
Barley stripe rust is caused by Puccinia striiformis f.sp. hordei, (Psh), occurs worldwide, and is a major disease in South Asia. The aim of this work was to identify and estimate effects of loci underlying quantitative resistance to rust at seedling and adult plant stages. HI-AM panel of 261 barley genotypes consisting of released cultivars from North and South America, Europe, Australia, advanced breeding lines, and local landraces from ICARDA barley program were screened at seedling and adult plant stages for resistance to Psh. Seedling resistance was evaluated with the five prevalent Psh races in India. Screening for the adult plant stage resistance was also performed in two different locations by inoculating with a mixture of the five races used for seedling screeing. The panel was genotyped using DaRT-Seq high-throughput genotyping platform. The genome-wide association mapping (GWAM) showed a total of 45 QTL located across the seven barley chromosomes for seedling resistance to the five races and 18 QTL for adult plant stage resistance. Common QTL for different races at seedling stage were found on all chromosomes except on chromosome 1H. Four common QTL associated with seedling and adult plant stage resistance were found on chromosomes 2, 5, and 6H. Moreover, one of the QTL located on the long arm of chromosome 5H showed stable effects across environments for adult plant stage resistance. Several QTL identified in this study were also reported before in bi-parental and association mapping populations studies validating current GWAM. However 15 new QTL were found at adult plant stage on all chromosomes except the 4H, explaining up to 36.79% of the variance. The promising QTL detected at both stages, once validated, can be used for MAS in Psh resistance breeding program globally.
Journal Article
Dibenzothiophene desulfurization in hydrocarbon environment by Staphylococcus sp. resting cells
by
Adhikari, D.K.
,
Verma, P.S.
,
Goindi, H.K.
in
Biological and medical sciences
,
Biology of microorganisms of confirmed or potential industrial interest
,
Biotechnology
2002
Staphylococcus sp. strain S3/C desulfurized dibenzothiophene/n-hexadecane (3 mg ml^sup -1^) in a hydrocarbon aqueous biphasic culture. The resting cells decreased the sulfur content of the hydrocarbon phase by 57% at 2.2 mg l^sup -1^ h^sup -1^ in the absence of any additional carbon and sulfur source.[PUBLICATION ABSTRACT]
Journal Article
A Cell Plate-Specific Callose Synthase and Its Interaction with Phragmoplastin
by
Delauney, Ashton J.
,
Hong, Zonglie
,
Desh Pal S. Verma
in
Amino Acid Motifs
,
Amino Acid Sequence
,
amino acid sequences
2001
Callose is synthesized on the forming cell plate and several other locations in the plant. We cloned an Arabidopsis cDNA encoding a callose synthase (CalS1) catalytic subunit. The CalS1 gene comprises 42 exons with 41 introns and is transcribed into a 6.0-kb mRNA. The deduced peptide, with an approximate molecular mass of 226 kD, showed sequence homology with the yeast 1,3-β-glucan synthases and is distinct from plant cellulose synthases. CalS1 contains 16 predicted transmembrane helices with the N-terminal region and a large central loop facing the cytoplasm. CalS1 interacts with two cell plate-associated proteins, phragmoplastin and a novel UDP-glucose transferase that copurifies with the CalS complex. That CalS1 is a cell plate-specific enzyme is demonstrated by the observations that the green fluorescent protein-CalS1 fusion protein was localized at the growing cell plate, that expression of CalS1 in transgenic tobacco cells enhanced callose synthesis on the forming cell plate, and that these cell lines exhibited higher levels of CalS activity. These data also suggest that plant CalS may form a complex with UDP-glucose transferase to facilitate the transfer of substrate for callose synthesis.
Journal Article
A Novel UDP-Glucose Transferase Is Part of the Callose Synthase Complex and Interacts with Phragmoplastin at the Forming Cell Plate
by
Desh Pal S. Verma
,
Olson, John M.
,
Hong, Zonglie
in
amino acid sequences
,
animal proteins
,
Antibodies
2001
Using phragmoplastin as a bait, we isolated an Arabidopsis cDNA encoding a novel UDP-glucose transferase (UGT1). This interaction was confirmed by an in vitro protein-protein interaction assay using purified UGT1 and radiolabeled phragmoplastin. Protein gel blot results revealed that UGT1 is associated with the membrane fraction and copurified with the product-entrapped callose synthase complex. These data suggest that UGT1 may act as a subunit of callose synthase that uses UDP-glucose to synthesize callose, a 1,3-β-glucan. UGT1 also interacted with Rop1, a Rho-like protein, and this interaction occurred only in its GTP-bound configuration, suggesting that the plant callose synthase may be regulated by Rop1 through the interaction with UGT1. The green fluorescent protein-UGT1 fusion protein was located on the forming cell plate during cytokinesis. We propose that UGT1 may transfer UDP-glucose from sucrose synthase to the callose synthase and thus help form a substrate channel for the synthesis of callose at the forming cell plate.
Journal Article
Virulence of Moroccan Pyrenophora teres f. teres Revealed by International Differential Barley Genotypes
by
Taibi, K.
,
Aissami, A. El
,
Labhilili, M.
in
Agriculture
,
agroecological zones
,
analysis of variance
2016
Pyrenophora teres f. teres (Ptt), causing net blotch in barley, is an important and frequently isolated leaf pathogen across the globe. The virulence spectrum of Ptt from North Africa including Morocco is poorly understood. Sixteen barley genotypes were challenged, at seedling stage, with 15 Ptt isolates that were collected from different agroecological zones of Morocco. The experiment was conducted in a factorial arrangement of treatments in a randomized complete block design with three replicates. The ANOVA revealed highly significant (P<0.001) effects of genotype (G), isolate (I) and G×I interaction explaining 23.2, 62.5, and 13.9% of the variation, respectively. Therefore, the current study revealed highly diverse virulence pattern of Moroccan isolates. Furthermore, the results indicated that minor virulence of Ptt isolates dominated over virulence interaction. In addition, Taffa (6-rowed) and Aglou (2 rowed), had the highest level of resistance to Ptt, while Coast and Rabat071 were the most susceptible genotypes. Pt2, Pt7, Pt8 and Pt4 were being the most virulent isolates, while Pt10 and Pt11 were the least virulent isolates. The emergence of the new Ptt pathotypes, which were highly virulent to durable resistance in Rabat071 posed a risk of breaking down the currently deployed resistance to net blotch in Morocco. A careful evaluation and selection of Ptt isolates based on minor virulence pattern to barley genotypes is essential for successful barley breeding program for resistance to net blotch in Morocco.
Journal Article
Breeding Barley for Malting Quality Improvement in India
2008
Grain and malt traits important for malting quality were studied on a set of 131 genotypes including two and six row types barley of indigenous and exotic origin grown at two locations for two seasons. Observations on seven grain and seven malt traits were recorded and malting was done with Phoenix® automatic micro-malting system. The correlation studies indicated that the hot water extract (HWE) is correlated with a number of grain (hectolitre weight, plump %, thin %, protein %, TGW and hull %) and malt (friability, homogeneity, wort viscosity, filtration rate and Kolbach Index) traits either positively or negatively. The multiple regression analysis indicated that hectolitre weight, TGW, hull content and malt friability can be used to predict HWE, the ultimately important trait with malting and brewing industry, in early generations of a breeding programme or for initial screening of germplasm accessions.
Journal Article